Pervasive transcription read-through promotes aberrant expression of oncogenes and RNA chimeras in renal carcinoma.

abstract：:A mathematical model shows how the shape of early multicellular organisms may have helped cells evolve specialized roles. ...

journal_title：eLife

authors： Staps M,Tarnita C

更新日期：2020-11-03 00:00:00

abstract：:Drosophila melanogaster males perform a series of courtship behaviors that, when successful, result in copulation with a female. For over a century, mutations in the yellow gene, named for its effects on pigmentation, have been known to reduce male mating success. Prior work has suggested that yellow influences mating...

journal_title：eLife

authors： Massey JH,Chung D,Siwanowicz I,Stern DL,Wittkopp PJ

更新日期：2019-10-15 00:00:00

abstract：:Malaria infection starts with injection of Plasmodium sporozoites by an Anopheles mosquito into the skin of the mammalian host. How sporozoites locate and enter a blood vessel is a critical, but poorly understood process. In this study, we examine sporozoite motility and their interaction with dermal blood vessels, us...

journal_title：eLife

authors： Hopp CS,Chiou K,Ragheb DR,Salman AM,Khan SM,Liu AJ,Sinnis P

更新日期：2015-08-13 00:00:00

abstract：:Recent work draws attention to community-community encounters ('coalescence') as likely an important factor shaping natural ecosystems. This work builds on MacArthur's classic model of competitive coexistence to investigate such community-level competition in a minimal theoretical setting. It is shown that the ability...

journal_title：eLife

authors： Tikhonov M

更新日期：2016-06-16 00:00:00

abstract：:Cardiac conduction defects decrease life expectancy in myotonic dystrophy type 1 (DM1), a CTG repeat disorder involving misbalance between two RNA binding factors, MBNL1 and CELF1. However, how DM1 condition translates into conduction disorders remains poorly understood. Here we simulated MBNL1 and CELF1 misbalance in...

journal_title：eLife

authors： Auxerre-Plantié E,Nakamori M,Renaud Y,Huguet A,Choquet C,Dondi C,Miquerol L,Takahashi MP,Gourdon G,Junion G,Jagla T,Zmojdzian M,Jagla K

更新日期：2019-12-12 00:00:00

abstract：:The speed at which a cell fate decision in nematode worms evolves is due to the number of genes that control the decision, rather than to a high mutation rate. ...

journal_title：eLife

authors： Yang B,Rifkin SA

更新日期：2020-10-14 00:00:00

abstract：:A spring-loaded mechanism can explain the activation process for a protein that has a crucial role in maintaining the genomic integrity of immature eggs cells. ...

journal_title：eLife

authors： Amelio I,Melino G

更新日期：2016-05-23 00:00:00

abstract：:The permeability barrier of nuclear pore complexes (NPCs) controls bulk nucleocytoplasmic exchange. It consists of nucleoporin domains rich in phenylalanine-glycine motifs (FG domains). As a bottom-up nanoscale model for the permeability barrier, we have used planar films produced with three different end-grafted FG d...

journal_title：eLife

authors： Zahn R,Osmanović D,Ehret S,Araya Callis C,Frey S,Stewart M,You C,Görlich D,Hoogenboom BW,Richter RP

更新日期：2016-04-08 00:00:00

abstract：:Improving the peer review process, overcoming the limitations of print journals and providing open access to the very best work in the life and biomedical sciences are three highlights of our first year. ...

journal_title：eLife

authors： Schekman R,Watt FM,Weigel D

更新日期：2013-10-15 00:00:00

abstract：:Studies of the performance of individuals with dyslexia in perceptual tasks suggest that their implicit inference of sound statistics is impaired. Previously, using two-tone frequency discrimination, we found that the effect of previous trials' frequencies on the judgments of individuals with dyslexia decays faster th...

journal_title：eLife

authors： Jaffe-Dax S,Kimel E,Ahissar M

更新日期：2018-02-28 00:00:00

abstract：:Translation is a core cellular process carried out by a highly conserved macromolecular machine, the ribosome. There has been remarkable evolutionary adaptation of this machine through the addition of eukaryote-specific ribosomal proteins whose individual effects on ribosome function are largely unknown. Here we show ...

journal_title：eLife

authors： Thompson MK,Rojas-Duran MF,Gangaramani P,Gilbert WV

更新日期：2016-04-27 00:00:00

abstract：:Some anaerobic bacteria use insoluble minerals as terminal electron acceptors and discovering the ways in which electrons move through the membrane barrier to the exterior acceptor forms an active field of research with implications for both bacterial physiology and bioenergy. A previous study suggested that Shewanell...

journal_title：eLife

authors： Mevers E,Su L,Pishchany G,Baruch M,Cornejo J,Hobert E,Dimise E,Ajo-Franklin CM,Clardy J

更新日期：2019-06-24 00:00:00

abstract：:In endoplasmic reticulum-associated protein degradation (ERAD), membrane proteins are ubiquitinated, extracted from the membrane, and degraded by the proteasome. The cytosolic ATPase Cdc48 drives extraction by pulling on polyubiquitinated substrates. How hydrophobic transmembrane (TM) segments are moved from the phosp...

journal_title：eLife

authors： Schmidt CC,Vasic V,Stein A

更新日期：2020-06-26 00:00:00

abstract：:Numerous brain regions have been shown to have neural correlates of gradually accumulating evidence for decision-making, but the causal roles of these regions in decisions driven by accumulation of evidence have yet to be determined. Here, in rats performing an auditory evidence accumulation task, we inactivated the f...

journal_title：eLife

authors： Erlich JC,Brunton BW,Duan CA,Hanks TD,Brody CD

更新日期：2015-04-14 00:00:00

abstract：:Although activation/deactivation of specific brain regions has been shown to be predictive of successful memory encoding, the relationship between time-varying large-scale brain networks and fluctuations of memory encoding performance remains unclear. Here, we investigated time-varying functional connectivity patterns...

journal_title：eLife

authors： Keerativittayayut R,Aoki R,Sarabi MT,Jimura K,Nakahara K

更新日期：2018-06-18 00:00:00

abstract：:We analyzed 2898 scientific papers published between 1995 and 2017 in which two or more authors shared the first author position. For papers in which the first and second authors made equal contributions, mixed-gender combinations were most frequent, followed by male-male and then female-female author combinations. Fo...

journal_title：eLife

authors： Broderick NA,Casadevall A

更新日期：2019-01-29 00:00:00

abstract：:Wnt plays important role during development and in various diseases. Because Wnts are lipidated and highly hydrophobic, they can only be purified in the presence of detergents, limiting their use in various in vitro and in vivo assays. We purified N-terminally tagged recombinant Wnt3a secreted from cells and accidenta...

journal_title：eLife

authors： Mihara E,Hirai H,Yamamoto H,Tamura-Kawakami K,Matano M,Kikuchi A,Sato T,Takagi J

更新日期：2016-02-23 00:00:00

abstract：:Exhaustion of stem cells is a hallmark of aging. In the Drosophila testis, dedifferentiated germline stem cells (GSCs) derived from spermatogonia increase during lifespan, leading to the model that dedifferentiation counteracts the decline of GSCs in aged males. To test this, we blocked dedifferentiation by mis-expres...

journal_title：eLife

authors： Herrera SC,Bach EA

更新日期：2018-07-09 00:00:00

abstract：:Neurotransmitter release requires formation of trans-SNARE complexes between the synaptic vesicle and plasma membranes, which likely underlies synaptic vesicle priming to a release-ready state. It is unknown whether Munc18-1, Munc13-1, complexin-1 and synaptotagmin-1 are important for priming because they mediate tran...

journal_title：eLife

authors： Prinslow EA,Stepien KP,Pan YZ,Xu J,Rizo J

更新日期：2019-01-18 00:00:00

abstract：:Iron storage proteins are essential for cellular iron homeostasis and redox balance. Ferritin proteins are the major storage units for bioavailable forms of iron. Some organisms lack ferritins, and it is not known how they store iron. Encapsulins, a class of protein-based organelles, have recently been implicated in m...

journal_title：eLife

authors： Giessen TW,Orlando BJ,Verdegaal AA,Chambers MG,Gardener J,Bell DC,Birrane G,Liao M,Silver PA

更新日期：2019-07-08 00:00:00

abstract：:Tuberculosis disproportionately affects the Canadian Inuit. To address this, it is imperative we understand transmission dynamics in this population. We investigate whether 'deep' sequencing can provide additional resolution compared to standard sequencing, using a well-characterized outbreak from the Arctic (2011-201...

journal_title：eLife

authors： Lee RS,Proulx JF,McIntosh F,Behr MA,Hanage WP

更新日期：2020-02-04 00:00:00

abstract：:Orphans are genes restricted to a single phylogenetic lineage and emerge at high rates. While this predicts an accumulation of genes, the gene number has remained remarkably constant through evolution. This paradox has not yet been resolved. Because orphan genes have been mainly analyzed over long evolutionary time sc...

journal_title：eLife

authors： Palmieri N,Kosiol C,Schlötterer C

更新日期：2014-02-19 00:00:00

abstract：:Protein aggregation appears to originate from partially unfolded conformations that are sampled through stochastic fluctuations of the native protein. It has been a challenge to characterize these fluctuations, under native like conditions. Here, the conformational dynamics of the full-length (23-231) mouse prion prot...

journal_title：eLife

authors： Goluguri RR,Sen S,Udgaonkar J

更新日期：2019-04-26 00:00:00

abstract：:One of the major unanswered questions in evolutionary biology is when and how the transition between diderm (two membranes) and monoderm (one membrane) cell envelopes occurred in Bacteria. The Negativicutes and the Halanaerobiales belong to the classically monoderm Firmicutes, but possess outer membranes with lipopoly...

journal_title：eLife

authors： Antunes LC,Poppleton D,Klingl A,Criscuolo A,Dupuy B,Brochier-Armanet C,Beloin C,Gribaldo S

更新日期：2016-08-31 00:00:00

abstract：:Hawaiian isolates of the nematode species Caenorhabditis elegans have long been known to harbor genetic diversity greater than the rest of the worldwide population, but this observation was supported by only a small number of wild strains. To better characterize the niche and genetic diversity of Hawaiian C. elegans a...

journal_title：eLife

authors： Crombie TA,Zdraljevic S,Cook DE,Tanny RE,Brady SC,Wang Y,Evans KS,Hahnel S,Lee D,Rodriguez BC,Zhang G,van der Zwagg J,Kiontke K,Andersen EC

更新日期：2019-12-03 00:00:00

abstract：:Cell growth and/or proliferation may require the reprogramming of metabolic pathways, whereby a switch from oxidative to glycolytic metabolism diverts glycolytic intermediates towards anabolic pathways. Herein, we identify a novel role for TRIM32 in the maintenance of glycolytic flux mediated by biochemical interactio...

journal_title：eLife

authors： Bawa S,Brooks DS,Neville KE,Tipping M,Sagar MA,Kollhoff JA,Chawla G,Geisbrecht BV,Tennessen JM,Eliceiri KW,Geisbrecht ER

更新日期：2020-03-30 00:00:00

abstract：:Coat protein complex II (COPII) mediates formation of the membrane vesicles that export newly synthesised proteins from the endoplasmic reticulum. The inner COPII proteins bind to cargo and membrane, linking them to the outer COPII components that form a cage around the vesicle. Regulated flexibility in coat architect...

journal_title：eLife

authors： Zanetti G,Prinz S,Daum S,Meister A,Schekman R,Bacia K,Briggs JA

更新日期：2013-09-17 00:00:00

abstract：:Genome-wide association studies identified the BIN1 locus as a leading modulator of genetic risk in Alzheimer's disease (AD). One limitation in understanding BIN1's contribution to AD is its unknown function in the brain. AD-associated BIN1 variants are generally noncoding and likely change expression. Here, we determ...

journal_title：eLife

authors： Voskobiynyk Y,Roth JR,Cochran JN,Rush T,Carullo NV,Mesina JS,Waqas M,Vollmer RM,Day JJ,McMahon LL,Roberson ED

更新日期：2020-07-13 00:00:00

abstract：:Variation in the presence or absence of transposable elements (TEs) is a major source of genetic variation between individuals. Here, we identified 23,095 TE presence/absence variants between 216 Arabidopsis accessions. Most TE variants were rare, and we find these rare variants associated with local extremes of gene ...

journal_title：eLife

authors： Stuart T,Eichten SR,Cahn J,Karpievitch YV,Borevitz JO,Lister R

更新日期：2016-12-02 00:00:00

abstract：:TMEM16A is a ligand-gated anion channel that is activated by intracellular Ca2+. This channel comprises two independent pores and closely apposed Ca2+ binding sites that are contained within each subunit of a homodimeric protein. Previously we characterized the influence of positively charged pore-lining residues on a...

journal_title：eLife

authors： Lam AK,Dutzler R

更新日期：2018-10-12 00:00:00