Conserved noncoding transcription and core promoter regulatory code in early Drosophila development.

abstract：:Plasticity can put evolution on repeat if development causes species to generate similar morphologies in similar environments. Anolis lizards offer the opportunity to put this role of developmental plasticity to the test. Following colonization of the four Greater Antillean islands, Anolis lizards independently and re...

journal_title：eLife

authors： Feiner N,Jackson IS,Munch KL,Radersma R,Uller T

更新日期：2020-08-13 00:00:00

abstract：:Experience-dependent modifications of synaptic connections are thought to change patterns of network activities and stimulus tuning with learning. However, only a few studies explored how synaptic plasticity shapes the response dynamics of cortical circuits. Here, we investigated the mechanism underlying sharpening of...

journal_title：eLife

authors： Lim S

更新日期：2019-08-08 00:00:00

abstract：:Peroxiredoxins are H2O2 scavenging enzymes that also carry out H2O2 signaling and chaperone functions. In yeast, the major cytosolic peroxiredoxin, Tsa1 is required for both promoting resistance to H2O2 and extending lifespan upon caloric restriction. We show here that Tsa1 effects both these functions not by scavengi...

journal_title：eLife

authors： Roger F,Picazo C,Reiter W,Libiad M,Asami C,Hanzén S,Gao C,Lagniel G,Welkenhuysen N,Labarre J,Nyström T,Grøtli M,Hartl M,Toledano MB,Molin M

更新日期：2020-07-14 00:00:00

abstract：:Whether somatic mutations contribute functional diversity to brain cells is a long-standing question. Single-neuron genomics enables direct measurement of somatic mutation rates in human brain and promises to answer this question. A recent study (Upton et al., 2015) reported high rates of somatic LINE-1 element (L1) r...

journal_title：eLife

authors： Evrony GD,Lee E,Park PJ,Walsh CA

更新日期：2016-02-22 00:00:00

abstract：:Imaging sperm as they travel through the female reproductive tract has revealed new details about fertilization at the molecular level. ...

journal_title：eLife

authors： Komondor KM,Carlson AE

更新日期：2020-12-02 00:00:00

abstract：:Dopamine and striatal dysfunctions play a key role in the pathophysiology of Parkinson's disease (PD) and Dystonia, but our understanding of the changes in the discharge rate and pattern of striatal projection neurons (SPNs) remains limited. Here, we recorded and examined multi-unit signals from the striatum of PD and...

journal_title：eLife

authors： Valsky D,Heiman Grosberg S,Israel Z,Boraud T,Bergman H,Deffains M

更新日期：2020-08-19 00:00:00

abstract：:Hepcidin is the master regulator of systemic iron homeostasis. Derived primarily from the liver, it inhibits the iron exporter ferroportin in the gut and spleen, the sites of iron absorption and recycling respectively. Recently, we demonstrated that ferroportin is also found in cardiomyocytes, and that its cardiac-spe...

journal_title：eLife

authors： Lakhal-Littleton S,Wolna M,Chung YJ,Christian HC,Heather LC,Brescia M,Ball V,Diaz R,Santos A,Biggs D,Clarke K,Davies B,Robbins PA

更新日期：2016-11-29 00:00:00

abstract：:We collated contact tracing data from COVID-19 clusters in Singapore and Tianjin, China and estimated the extent of pre-symptomatic transmission by estimating incubation periods and serial intervals. The mean incubation periods accounting for intermediate cases were 4.91 days (95%CI 4.35, 5.69) and 7.54 (95%CI 6.76, 8...

journal_title：eLife

authors： Tindale LC,Stockdale JE,Coombe M,Garlock ES,Lau WYV,Saraswat M,Zhang L,Chen D,Wallinga J,Colijn C

更新日期：2020-06-22 00:00:00

abstract：:Recently, a small-molecule communication mechanism was discovered in a range of Bacillus-infecting bacteriophages, which these temperate phages use to inform their lysis-lysogeny decision. We present a mathematical model of the ecological and evolutionary dynamics of such viral communication, and show that a communica...

journal_title：eLife

authors： Doekes HM,Mulder GA,Hermsen R

更新日期：2021-01-18 00:00:00

abstract：:Experiments on mitochondrial DNA in worms highlight that cheating does not always pay off. ...

journal_title：eLife

authors： Camus MF

更新日期：2020-09-22 00:00:00

abstract：:The CRISPR-Cas9 bacterial surveillance system has become a versatile tool for genome editing and gene regulation in eukaryotic cells, yet how CRISPR-Cas9 contends with the barriers presented by eukaryotic chromatin is poorly understood. Here we investigate how the smallest unit of chromatin, a nucleosome, constrains t...

journal_title：eLife

authors： Isaac RS,Jiang F,Doudna JA,Lim WA,Narlikar GJ,Almeida R

更新日期：2016-04-28 00:00:00

abstract：:Understanding how virus capsids assemble around their nucleic acid (NA) genomes could promote efforts to block viral propagation or to reengineer capsids for gene therapy applications. We develop a coarse-grained model of capsid proteins and NAs with which we investigate assembly dynamics and thermodynamics. In contra...

journal_title：eLife

authors： Perlmutter JD,Qiao C,Hagan MF

更新日期：2013-06-14 00:00:00

abstract：: ...

journal_title：eLife

authors： MacKay CE,Leo MD,Fernández-Peña C,Hasan R,Yin W,Mata-Daboin A,Bulley S,Gammons J,Mancarella S,Jaggar JH

更新日期：2020-06-30 00:00:00

abstract：:Replication-dependent (RD) core histone mRNA produced during S-phase is the only known metazoan protein-coding mRNA presenting a 3' stem-loop instead of the otherwise universal polyA tail. A metallo β-lactamase (MBL) fold enzyme, cleavage and polyadenylation specificity factor 73 (CPSF73), is proposed to be the sole e...

journal_title：eLife

authors： Pettinati I,Grzechnik P,Ribeiro de Almeida C,Brem J,McDonough MA,Dhir S,Proudfoot NJ,Schofield CJ

更新日期：2018-12-03 00:00:00

abstract：:Antiviral effectors such as natural killer (NK) cells have impaired functions in chronic hepatitis B (CHB) patients. The molecular mechanism responsible for this dysfunction remains poorly characterised. We show that decreased cytokine production capacity of peripheral NK cells from CHB patients was associated with re...

journal_title：eLife

authors： Marotel M,Villard M,Drouillard A,Tout I,Besson L,Allatif O,Pujol M,Rocca Y,Ainouze M,Roblot G,Viel S,Gomez M,Loustaud V,Alain S,Durantel D,Walzer T,Hasan U,Marçais A

更新日期：2021-01-28 00:00:00

abstract：:Understanding genome to phenotype linkages has been greatly enabled by genomic sequencing. However, most genome analysis is typically confined to the nuclear genome. We conducted a metabolomic QTL analysis on a reciprocal RIL population structured to examine how variation in the organelle genomes affects phenotypic va...

journal_title：eLife

authors： Joseph B,Corwin JA,Li B,Atwell S,Kliebenstein DJ

更新日期：2013-10-08 00:00:00

abstract：:Using knowledge of the structure of the world to infer value is at the heart of model-based reasoning and relies on a circuit that includes the orbitofrontal cortex (OFC). Some accounts link this to the representation of biological significance or value by neurons in OFC, while other models focus on the representation...

journal_title：eLife

authors： Sadacca BF,Wied HM,Lopatina N,Saini GK,Nemirovsky D,Schoenbaum G

更新日期：2018-03-07 00:00:00

abstract：:A universal and unquestioned characteristic of eukaryotic cells is that the genome is divided into multiple chromosomes and encapsulated in a single nucleus. However, the underlying mechanism to ensure such a configuration is unknown. Here, we provide evidence that pericentromeric satellite DNA, which is often regarde...

journal_title：eLife

authors： Jagannathan M,Cummings R,Yamashita YM

更新日期：2018-03-26 00:00:00

abstract：:Many primary sensory cilia exhibit unique architectures that are critical for transduction of specific sensory stimuli. Although basic ciliogenic mechanisms are well described, how complex ciliary structures are generated remains unclear. Seminal work performed several decades ago provided an initial but incomplete de...

journal_title：eLife

authors： Doroquez DB,Berciu C,Anderson JR,Sengupta P,Nicastro D

更新日期：2014-03-25 00:00:00

abstract：:Cells deficient in the pro-death Bcl-2 family members Bax and Bak are known to be resistant to apoptotic cell death, and previous we have shown that these two effectors are also needed for mitochondrial-dependent cellular necrosis (Karch et al., 2013). Here we show that mouse embryonic fibroblasts deficient in Bax/Bak...

journal_title：eLife

authors： Karch J,Schips TG,Maliken BD,Brody MJ,Sargent MA,Kanisicak O,Molkentin JD

更新日期：2017-11-17 00:00:00

abstract：:Obesity produces a chronic inflammatory state involving the NFκB pathway, resulting in persistent elevation of the noncanonical IκB kinases IKKε and TBK1. In this study, we report that these kinases attenuate β-adrenergic signaling in white adipose tissue. Treatment of 3T3-L1 adipocytes with specific inhibitors of the...

journal_title：eLife

authors： Mowers J,Uhm M,Reilly SM,Simon J,Leto D,Chiang SH,Chang L,Saltiel AR

更新日期：2013-12-24 00:00:00

abstract：:The knirps (kni) locus encodes transcription factors required for induction of the L2 wing vein in Drosophila. Here, we employ diverse CRISPR/Cas9 genome editing tools to generate a series of targeted lesions within the endogenous cis-regulatory module (CRM) required for kni expression in the L2 vein primordium. Pheno...

journal_title：eLife

authors： Xu XS,Gantz VM,Siomava N,Bier E

更新日期：2017-12-23 00:00:00

abstract：:Computational models postulate that head-direction (HD) cells are part of an attractor network integrating head turns. This network requires inputs from visual landmarks to anchor the HD signal to the external world. We investigated whether information about HD and visual landmarks is integrated in the medial entorhin...

journal_title：eLife

authors： Kornienko O,Latuske P,Bassler M,Kohler L,Allen K

更新日期：2018-09-17 00:00:00

abstract：:Sensory navigation results from coordinated transitions between distinct behavioral programs. During chemotaxis in the Drosophila melanogaster larva, the detection of positive odor gradients extends runs while negative gradients promote stops and turns. This algorithm represents a foundation for the control of sensory...

journal_title：eLife

authors： Tastekin I,Khandelwal A,Tadres D,Fessner ND,Truman JW,Zlatic M,Cardona A,Louis M

更新日期：2018-11-22 00:00:00

abstract：:AMPARs control fast synaptic communication between neurons and their function relies on auxiliary subunits, which importantly modulate channel properties. Although it has been suggested that AMPARs can bind to TARPs with variable stoichiometry, little is known about the effect that this stoichiometry exerts on certain...

journal_title：eLife

authors： Miguez-Cabello F,Sánchez-Fernández N,Yefimenko N,Gasull X,Gratacòs-Batlle E,Soto D

更新日期：2020-05-26 00:00:00

abstract：:Our knowledge about the repertoire of ribosomal RNA modifications and the enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 is responsible for depositing m5C at position C2381 on the 26S rRNA in Caenorhabditis elegans. Here, we show that NSUN-1 is writing the second k...

journal_title：eLife

authors： Heissenberger C,Rollins JA,Krammer TL,Nagelreiter F,Stocker I,Wacheul L,Shpylovyi A,Tav K,Snow S,Grillari J,Rogers AN,Lafontaine DLJ,Schosserer M

更新日期：2020-12-08 00:00:00

abstract：:Oligomeric assemblies of intraflagellar transport (IFT) particles build cilia through sequential recruitment and transport of ciliary cargo proteins within cilia. Here we present the 1.8 Å resolution crystal structure of the Chlamydomonas IFT-B protein IFT80, which reveals the architecture of two N-terminal β-propelle...

journal_title：eLife

authors： Taschner M,Lorentzen A,Mourão A,Collins T,Freke GM,Moulding D,Basquin J,Jenkins D,Lorentzen E

更新日期：2018-04-16 00:00:00

abstract：:With implantation, mouse stromal cells begin to transform into epithelial-like cells surrounding the implantation chamber forming an avascular zone called the primary decidual zone (PDZ). In the mouse, the PDZ forms a transient, size-dependent permeable barrier to protect the embryo from maternal circulating harmful a...

journal_title：eLife

authors： Li Y,Dewar A,Kim YS,Dey SK,Sun X

更新日期：2020-09-29 00:00:00

abstract：:Ageing is characterised by cellular senescence, leading to imbalanced tissue maintenance, cell death and compromised organ function. This is first observed in the thymus, the primary lymphoid organ that generates and selects T cells. However, the molecular and cellular mechanisms underpinning these ageing processes re...

journal_title：eLife

authors： Baran-Gale J,Morgan MD,Maio S,Dhalla F,Calvo-Asensio I,Deadman ME,Handel AE,Maynard A,Chen S,Green F,Sit RV,Neff NF,Darmanis S,Tan W,May AP,Marioni JC,Ponting CP,Holländer GA

更新日期：2020-08-25 00:00:00

abstract：:Ageing is a progressive decline of intrinsic physiological functions. We examined the impact of ageing on the ultrastructure and function of mitochondria in mouse and fruit flies (Drosophila melanogaster) by electron cryo-tomography and respirometry. We discovered distinct age-related changes in both model organisms. ...

journal_title：eLife

authors： Brandt T,Mourier A,Tain LS,Partridge L,Larsson NG,Kühlbrandt W

更新日期：2017-07-12 00:00:00