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eLife文献大全abstract::Energy metabolism is essential for T cell function. However, how persistent antigenic stimulation affects T cell metabolism is unknown. Here, we report that long-term in vivo antigenic exposure induced a specific deficit in numerous metabolic enzymes. Accordingly, T cells exhibited low basal glycolytic flux and limite...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30938
更新日期:2018-06-18 00:00:00
abstract::Positive-strand RNA viruses, the largest genetic class of viruses, include numerous important pathogens such as Zika virus. These viruses replicate their RNA genomes in novel, membrane-bounded mini-organelles, but the organization of viral proteins and RNAs in these compartments has been largely unknown. We used cryo-...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25940
更新日期:2017-06-27 00:00:00
abstract::Acquisition of pluripotency is driven largely at the transcriptional level by activators OCT4, SOX2, and NANOG that must in turn cooperate with diverse coactivators to execute stem cell-specific gene expression programs. Using a biochemically defined in vitro transcription system that mediates OCT4/SOX2 and coactivato...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03573
更新日期:2014-11-19 00:00:00
abstract::Unrepaired DNA damage during embryonic development can be potentially inherited by a large population of cells. However, the quality control mechanisms that minimize the contribution of damaged cells to developing embryos remain poorly understood. Here, we uncovered an ATR- and CHK1-mediated transcriptional response t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54756
更新日期:2020-03-12 00:00:00
abstract::Although it is clear that trisomy 21 causes Down syndrome, the molecular events acting downstream of the trisomy remain ill defined. Using complementary genomics analyses, we identified the interferon pathway as the major signaling cascade consistently activated by trisomy 21 in human cells. Transcriptome analysis rev...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.16220
更新日期:2016-07-29 00:00:00
abstract::The numerous neurons and glia that form the brain originate from tightly controlled growth and division of neural stem cells, regulated systemically by important known stem cell-extrinsic signals. However, the cell-intrinsic mechanisms that control the distinctive proliferation rates of individual neural stem cells ar...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51529
更新日期:2020-01-14 00:00:00
abstract::Evolution persistently undermines vector control programs through insecticide resistance. Here we propose a novel strategy which instead exploits evolution to generate and sustain new control tools. Effective spatial repellents are needed to keep vectors out of houses. Our approach generates such new repellents by com...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15416
更新日期:2016-10-25 00:00:00
abstract::Cells can avoid the effects of so-called 'nonsense' mutations by several methods, including a newly discovered mechanism driven by microRNA molecules. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.04300
更新日期:2014-09-09 00:00:00
abstract::Periodontal disease is an age-associated disorder clinically defined by periodontal bone loss, inflammation of the specialized tissues that surround and support the tooth, and microbiome dysbiosis. Currently, there is no therapy for reversing periodontal disease, and treatment is generally restricted to preventive mea...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54318
更新日期:2020-04-28 00:00:00
abstract::In eukaryotes, intra-chromosomal recombination generates DNA circles, but little is known about how cells react to them. In yeast, partitioning of such circles to the mother cell at mitosis ensures their loss from the population but promotes replicative ageing. Nevertheless, the mechanisms of partitioning are debated....
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03790
更新日期:2014-11-17 00:00:00
abstract::How different helicase families with a conserved catalytic 'helicase core' evolved to function on varied RNA and DNA substrates by diverse mechanisms remains unclear. In this study, we used Mss116, a yeast DEAD-box protein that utilizes ATP to locally unwind dsRNA, to investigate helicase specificity and mechanism. Ou...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.04630
更新日期:2014-12-12 00:00:00
abstract::Though neurotransmitters are essential elements in neuronal signal transduction, techniques for in vivo analysis are still limited. Here, we describe an organic electrochemical transistor array (OECT-array) technique for monitoring catecholamine neurotransmitters (CA-NTs) in rat brains. The OECT-array is an active sen...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50345
更新日期:2020-02-11 00:00:00
abstract::Compartmentalized oscillations of second messengers affect global cellular signaling. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.63845
更新日期:2020-11-17 00:00:00
abstract::AMPARs control fast synaptic communication between neurons and their function relies on auxiliary subunits, which importantly modulate channel properties. Although it has been suggested that AMPARs can bind to TARPs with variable stoichiometry, little is known about the effect that this stoichiometry exerts on certain...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53946
更新日期:2020-05-26 00:00:00
abstract::Antiviral effectors such as natural killer (NK) cells have impaired functions in chronic hepatitis B (CHB) patients. The molecular mechanism responsible for this dysfunction remains poorly characterised. We show that decreased cytokine production capacity of peripheral NK cells from CHB patients was associated with re...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60095
更新日期:2021-01-28 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease involving cytotoxic conformations of Cu, Zn superoxide dismutase (SOD1). A major challenge in understanding ALS disease pathology has been the identification and atomic-level characterization of these conformers. Here, we use a combination ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.07296
更新日期:2015-06-23 00:00:00
abstract::The TP53 tumor-suppressor gene is mutated in >50% of human tumors and Li-Fraumeni patients with germ line inactivation are predisposed to developing cancer. Here, we generated tp53 deleted zebrafish that spontaneously develop malignant peripheral nerve-sheath tumors, angiosarcomas, germ cell tumors, and an aggressive ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.37202
更新日期:2018-09-07 00:00:00
abstract::With implantation, mouse stromal cells begin to transform into epithelial-like cells surrounding the implantation chamber forming an avascular zone called the primary decidual zone (PDZ). In the mouse, the PDZ forms a transient, size-dependent permeable barrier to protect the embryo from maternal circulating harmful a...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.61762
更新日期:2020-09-29 00:00:00
abstract::Repression of genes by Polycomb requires that PRC2 modifies their chromatin by trimethylating lysine 27 on histone H3 (H3K27me3). At transcriptionally active genes, di- and tri-methylated H3K36 inhibit PRC2. Here, the cryo-EM structure of PRC2 on dinucleosomes reveals how binding of its catalytic subunit EZH2 to nucle...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.61964
更新日期:2020-11-19 00:00:00
abstract::Binary fission has been well studied in rod-shaped bacteria, but the mechanisms underlying cell division in spherical bacteria are poorly understood. Rod-shaped bacteria harbor regulatory proteins that place and remodel the division machinery during cytokinesis. In the spherical human pathogen Staphylococcus aureus, w...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.38856
更新日期:2018-10-02 00:00:00
abstract::An individual may need to take different actions to the same stimulus in different situations to achieve a given goal. The selection of the appropriate action hinges on the previously learned associations between stimuli, actions, and outcomes in the situations. Here, using a go/no-go paradigm and a symmetrical reward...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.43281
更新日期:2019-04-04 00:00:00
abstract::Pioneer factors such as Zelda (Zld) help initiate zygotic transcription in Drosophila early embryos, but whether other factors support this dynamic process is unclear. Odd-paired (Opa), a zinc-finger transcription factor expressed at cellularization, controls the transition of genes from pair-rule to segmental pattern...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.59610
更新日期:2020-07-23 00:00:00
abstract::Cavin-3 regulates metabolism and cell proliferation by coordinating the activities of growth factor signalling cascades. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.01428
更新日期:2013-09-24 00:00:00
abstract::Apical constriction is a widely utilized cell shape change linked to folding, bending and invagination of polarized epithelia. It remains unclear how apical constriction is regulated spatiotemporally during tissue invagination and how this cellular process contributes to tube formation in different developmental conte...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22235
更新日期:2017-03-06 00:00:00
abstract::The COVID-19 pandemic is a harsh reminder of the fact that, whether in a single human host or a wave of infection across continents, viral dynamics is often a story about the numbers. In this article we provide a one-stop, curated graphical source for the key numbers (based mostly on the peer-reviewed literature) abou...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57309
更新日期:2020-04-02 00:00:00
abstract::Voltage-gated potassium (Kv) channels enable potassium efflux and membrane repolarization in excitable tissues. Many Kv channels undergo a progressive loss of ion conductance in the presence of a prolonged voltage stimulus, termed slow inactivation, but the atomic determinants that regulate the kinetics of this proces...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01289
更新日期:2013-12-10 00:00:00
abstract::Rhythmic actions benefit from synchronization with external events. Auditory-paced finger tapping studies indicate the two cerebral hemispheres preferentially control different rhythms. It is unclear whether left-lateralized processing of faster rhythms and right-lateralized processing of slower rhythms bases upon hem...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.48404
更新日期:2019-11-07 00:00:00
abstract::Episodic memories reflect a bound representation of multimodal features that can be reinstated with varying precision. Yet little is known about how brain networks involved in memory, including the hippocampus and posterior-medial (PM) and anterior-temporal (AT) systems, interact to support the quality and content of ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45591
更新日期:2019-03-22 00:00:00
abstract::The time it takes a sound to travel from source to ear differs between the ears and creates an interaural delay. It varies systematically with spatial direction and is generally modeled as a pure time delay, independent of frequency. In acoustical recordings, we found that interaural delay varies with frequency at a f...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.06072
更新日期:2015-04-27 00:00:00
abstract::In vivo, the primary molecular mechanotransductive events mechanically initiating cell differentiation remain unknown. Here we find the molecular stretching of the highly conserved Y654-β-catenin-D665-E-cadherin binding site as mechanically induced by tissue strain. It triggers the increase of accessibility of the Y65...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.33381
更新日期:2018-07-19 00:00:00