Role of SAGA in the asymmetric segregation of DNA circles during yeast ageing.

Abstract:

:In eukaryotes, intra-chromosomal recombination generates DNA circles, but little is known about how cells react to them. In yeast, partitioning of such circles to the mother cell at mitosis ensures their loss from the population but promotes replicative ageing. Nevertheless, the mechanisms of partitioning are debated. In this study, we show that the SAGA complex mediates the interaction of non-chromosomal DNA circles with nuclear pore complexes (NPCs) and thereby promotes their confinement in the mother cell. Reciprocally, this causes retention and accumulation of NPCs, which affects the organization of ageing nuclei. Thus, SAGA prevents the spreading of DNA circles by linking them to NPCs, but unavoidably causes accumulation of circles and NPCs in the mother cell, and thereby promotes ageing. Together, our data provide a unifying model for the asymmetric segregation of DNA circles and how age affects nuclear organization.

journal_name

Elife

journal_title

eLife

authors

Denoth-Lippuner A,Krzyzanowski MK,Stober C,Barral Y

doi

10.7554/eLife.03790

subject

Has Abstract

pub_date

2014-11-17 00:00:00

issn

2050-084X

journal_volume

3

pub_type

杂志文章

相关文献

eLife文献大全
  • Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration.

    abstract::Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and rea...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.10230

    authors: Rodrigo Albors A,Tazaki A,Rost F,Nowoshilow S,Chara O,Tanaka EM

    更新日期:2015-11-14 00:00:00

  • Biosynthesis of histone messenger RNA employs a specific 3' end endonuclease.

    abstract::Replication-dependent (RD) core histone mRNA produced during S-phase is the only known metazoan protein-coding mRNA presenting a 3' stem-loop instead of the otherwise universal polyA tail. A metallo β-lactamase (MBL) fold enzyme, cleavage and polyadenylation specificity factor 73 (CPSF73), is proposed to be the sole e...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.39865

    authors: Pettinati I,Grzechnik P,Ribeiro de Almeida C,Brem J,McDonough MA,Dhir S,Proudfoot NJ,Schofield CJ

    更新日期:2018-12-03 00:00:00

  • On the trail of a tropical disease.

    abstract::Learning more about the cells that enable parasitic worms called schistosomes to reproduce inside snails could lead to new treatments that prevent these parasites from being transmitted to humans. ...

    journal_title:eLife

    pub_type: 评论,杂志文章

    doi:10.7554/eLife.01115

    authors: Sánchez Alvarado A

    更新日期:2013-07-30 00:00:00

  • Nuclear genetic regulation of the human mitochondrial transcriptome.

    abstract::Mitochondria play important roles in cellular processes and disease, yet little is known about how the transcriptional regime of the mitochondrial genome varies across individuals and tissues. By analyzing >11,000 RNA-sequencing libraries across 36 tissue/cell types, we find considerable variation in mitochondrial-enc...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.41927

    authors: Ali AT,Boehme L,Carbajosa G,Seitan VC,Small KS,Hodgkinson A

    更新日期:2019-02-18 00:00:00

  • TAPBPR alters MHC class I peptide presentation by functioning as a peptide exchange catalyst.

    abstract::Our understanding of the antigen presentation pathway has recently been enhanced with the identification that the tapasin-related protein TAPBPR is a second major histocompatibility complex (MHC) class I-specific chaperone. We sought to determine whether, like tapasin, TAPBPR can also influence MHC class I peptide sel...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.09617

    authors: Hermann C,van Hateren A,Trautwein N,Neerincx A,Duriez PJ,Stevanović S,Trowsdale J,Deane JE,Elliott T,Boyle LH

    更新日期:2015-10-06 00:00:00

  • Nuclear receptor LRH-1/NR5A2 is required and targetable for liver endoplasmic reticulum stress resolution.

    abstract::Chronic endoplasmic reticulum (ER) stress results in toxicity that contributes to multiple human disorders. We report a stress resolution pathway initiated by the nuclear receptor LRH-1 that is independent of known unfolded protein response (UPR) pathways. Like mice lacking primary UPR components, hepatic Lrh-1-null m...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.01694

    authors: Mamrosh JL,Lee JM,Wagner M,Stambrook PJ,Whitby RJ,Sifers RN,Wu SP,Tsai MJ,Demayo FJ,Moore DD

    更新日期:2014-04-15 00:00:00

  • Drosophila TRIM32 cooperates with glycolytic enzymes to promote cell growth.

    abstract::Cell growth and/or proliferation may require the reprogramming of metabolic pathways, whereby a switch from oxidative to glycolytic metabolism diverts glycolytic intermediates towards anabolic pathways. Herein, we identify a novel role for TRIM32 in the maintenance of glycolytic flux mediated by biochemical interactio...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.52358

    authors: Bawa S,Brooks DS,Neville KE,Tipping M,Sagar MA,Kollhoff JA,Chawla G,Geisbrecht BV,Tennessen JM,Eliceiri KW,Geisbrecht ER

    更新日期:2020-03-30 00:00:00

  • Transient inhibition and long-term facilitation of locomotion by phasic optogenetic activation of serotonin neurons.

    abstract::Serotonin (5-HT) is associated with mood and motivation but the function of endogenous 5-HT remains controversial. Here, we studied the impact of phasic optogenetic activation of 5-HT neurons in mice over time scales from seconds to weeks. We found that activating dorsal raphe nucleus (DRN) 5-HT neurons induced a stro...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.20975

    authors: Correia PA,Lottem E,Banerjee D,Machado AS,Carey MR,Mainen ZF

    更新日期:2017-02-14 00:00:00

  • Engineering induction of singular neural rosette emergence within hPSC-derived tissues.

    abstract::Human pluripotent stem cell (hPSC)-derived neural organoids display unprecedented emergent properties. Yet in contrast to the singular neuroepithelial tube from which the entire central nervous system (CNS) develops in vivo, current organoid protocols yield tissues with multiple neuroepithelial units, a.k.a. neural ro...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.37549

    authors: Knight GT,Lundin BF,Iyer N,Ashton LM,Sethares WA,Willett RM,Ashton RS

    更新日期:2018-10-29 00:00:00

  • Inhibition of protein synthesis in M1 of monkeys disrupts performance of sequential movements guided by memory.

    abstract::The production of action sequences is a fundamental aspect of motor skills. To examine whether primary motor cortex (M1) is involved in maintenance of sequential movements, we trained two monkeys (Cebus apella) to perform two sequential reaching tasks. In one task, sequential movements were instructed by visual cues, ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.53038

    authors: Ohbayashi M

    更新日期:2020-02-10 00:00:00

  • Assembling the Tat protein translocase.

    abstract::The twin-arginine protein translocation system (Tat) transports folded proteins across the bacterial cytoplasmic membrane and the thylakoid membranes of plant chloroplasts. The Tat transporter is assembled from multiple copies of the membrane proteins TatA, TatB, and TatC. We combine sequence co-evolution analysis, mo...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.20718

    authors: Alcock F,Stansfeld PJ,Basit H,Habersetzer J,Baker MA,Palmer T,Wallace MI,Berks BC

    更新日期:2016-12-03 00:00:00

  • A composite double-/single-stranded RNA-binding region in protein Prp3 supports tri-snRNP stability and splicing.

    abstract::Prp3 is an essential U4/U6 di-snRNP-associated protein whose functions and molecular mechanisms in pre-mRNA splicing are presently poorly understood. We show by structural and biochemical analyses that Prp3 contains a bipartite U4/U6 di-snRNA-binding region comprising an expanded ferredoxin-like fold, which recognizes...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.07320

    authors: Liu S,Mozaffari-Jovin S,Wollenhaupt J,Santos KF,Theuser M,Dunin-Horkawicz S,Fabrizio P,Bujnicki JM,Lührmann R,Wahl MC

    更新日期:2015-07-10 00:00:00

  • Pat1 promotes processing body assembly by enhancing the phase separation of the DEAD-box ATPase Dhh1 and RNA.

    abstract::Processing bodies (PBs) are cytoplasmic mRNP granules that assemble via liquid-liquid phase separation and are implicated in the decay or storage of mRNAs. How PB assembly is regulated in cells remains unclear. Previously, we identified the ATPase activity of the DEAD-box protein Dhh1 as a key regulator of PB dynamics...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.41415

    authors: Sachdev R,Hondele M,Linsenmeier M,Vallotton P,Mugler CF,Arosio P,Weis K

    更新日期:2019-01-16 00:00:00

  • Single-cell RNA-sequencing reveals distinct patterns of cell state heterogeneity in mouse models of breast cancer.

    abstract::Breast cancer stem cells (BCSCs) contribute to intra-tumoral heterogeneity and therapeutic resistance. However, the binary concept of universal BCSCs co-existing with bulk tumor cells is over-simplified. Through single-cell RNA-sequencing, we found that Neu, PyMT and BRCA1-null mammary tumors each corresponded to a sp...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.58810

    authors: Yeo SK,Zhu X,Okamoto T,Hao M,Wang C,Lu P,Lu LJ,Guan JL

    更新日期:2020-08-25 00:00:00

  • Distinct contributions of the thin and thick filaments to length-dependent activation in heart muscle.

    abstract::The Frank-Starling relation is a fundamental auto-regulatory property of the heart that ensures the volume of blood ejected in each heartbeat is matched to the extent of venous filling. At the cellular level, heart muscle cells generate higher force when stretched, but despite intense efforts the underlying molecular ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.24081

    authors: Zhang X,Kampourakis T,Yan Z,Sevrieva I,Irving M,Sun YB

    更新日期:2017-02-23 00:00:00

  • C. elegans neurons have functional dendritic spines.

    abstract::Dendritic spines are specialized postsynaptic structures that transduce presynaptic signals, are regulated by neural activity and correlated with learning and memory. Most studies of spine function have focused on the mammalian nervous system. However, spine-like protrusions have been reported in C. elegans (Philbrook...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.47918

    authors: Cuentas-Condori A,Mulcahy B,He S,Palumbos S,Zhen M,Miller DM 3rd

    更新日期:2019-10-04 00:00:00

  • Cryo-electron tomography reveals novel features of a viral RNA replication compartment.

    abstract::Positive-strand RNA viruses, the largest genetic class of viruses, include numerous important pathogens such as Zika virus. These viruses replicate their RNA genomes in novel, membrane-bounded mini-organelles, but the organization of viral proteins and RNAs in these compartments has been largely unknown. We used cryo-...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.25940

    authors: Ertel KJ,Benefield D,Castaño-Diez D,Pennington JG,Horswill M,den Boon JA,Otegui MS,Ahlquist P

    更新日期:2017-06-27 00:00:00

  • Quantitative properties of a feedback circuit predict frequency-dependent pattern separation.

    abstract::Feedback inhibitory motifs are thought to be important for pattern separation across species. How feedback circuits may implement pattern separation of biologically plausible, temporally structured input in mammals is, however, poorly understood. We have quantitatively determined key properties of netfeedback inhibiti...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.53148

    authors: Braganza O,Mueller-Komorowska D,Kelly T,Beck H

    更新日期:2020-02-20 00:00:00

  • Molecular and anatomical organization of the dorsal raphe nucleus.

    abstract::The dorsal raphe nucleus (DRN) is an important source of neuromodulators and has been implicated in a wide variety of behavioral and neurological disorders. The DRN is subdivided into distinct anatomical subregions comprised of multiple cell types, and its complex cellular organization has impeded efforts to investiga...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.46464

    authors: Huang KW,Ochandarena NE,Philson AC,Hyun M,Birnbaum JE,Cicconet M,Sabatini BL

    更新日期:2019-08-14 00:00:00

  • SARS-CoV-2 (COVID-19) by the numbers.

    abstract::The COVID-19 pandemic is a harsh reminder of the fact that, whether in a single human host or a wave of infection across continents, viral dynamics is often a story about the numbers. In this article we provide a one-stop, curated graphical source for the key numbers (based mostly on the peer-reviewed literature) abou...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.57309

    authors: Bar-On YM,Flamholz A,Phillips R,Milo R

    更新日期:2020-04-02 00:00:00

  • An ancient Pygo-dependent Wnt enhanceosome integrated by Chip/LDB-SSDP.

    abstract::TCF/LEF factors are ancient context-dependent enhancer-binding proteins that are activated by β-catenin following Wnt signaling. They control embryonic development and adult stem cell compartments, and their dysregulation often causes cancer. β-catenin-dependent transcription relies on the NPF motif of Pygo proteins. ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.09073

    authors: Fiedler M,Graeb M,Mieszczanek J,Rutherford TJ,Johnson CM,Bienz M

    更新日期:2015-08-27 00:00:00

  • HIV-1 integrase tetramers are the antiviral target of pyridine-based allosteric integrase inhibitors.

    abstract::Allosteric HIV-1 integrase (IN) inhibitors (ALLINIs) are a promising new class of antiretroviral agents that disrupt proper viral maturation by inducing hyper-multimerization of IN. Here we show that lead pyridine-based ALLINI KF116 exhibits striking selectivity for IN tetramers versus lower order protein oligomers. I...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.46344

    authors: Koneru PC,Francis AC,Deng N,Rebensburg SV,Hoyte AC,Lindenberger J,Adu-Ampratwum D,Larue RC,Wempe MF,Engelman AN,Lyumkis D,Fuchs JR,Levy RM,Melikyan GB,Kvaratskhelia M

    更新日期:2019-05-23 00:00:00

  • Structure-based characterization of novel TRPV5 inhibitors.

    abstract::Transient receptor potential vanilloid 5 (TRPV5) is a highly calcium selective ion channel that acts as the rate-limiting step of calcium reabsorption in the kidney. The lack of potent, specific modulators of TRPV5 has limited the ability to probe the contribution of TRPV5 in disease phenotypes such as hypercalcemia a...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.49572

    authors: Hughes TE,Del Rosario JS,Kapoor A,Yazici AT,Yudin Y,Fluck EC 3rd,Filizola M,Rohacs T,Moiseenkova-Bell VY

    更新日期:2019-10-25 00:00:00

  • An essential cell-autonomous role for hepcidin in cardiac iron homeostasis.

    abstract::Hepcidin is the master regulator of systemic iron homeostasis. Derived primarily from the liver, it inhibits the iron exporter ferroportin in the gut and spleen, the sites of iron absorption and recycling respectively. Recently, we demonstrated that ferroportin is also found in cardiomyocytes, and that its cardiac-spe...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.19804

    authors: Lakhal-Littleton S,Wolna M,Chung YJ,Christian HC,Heather LC,Brescia M,Ball V,Diaz R,Santos A,Biggs D,Clarke K,Davies B,Robbins PA

    更新日期:2016-11-29 00:00:00

  • Generalization of learned responses in the mormyrid electrosensory lobe.

    abstract::Appropriate generalization of learned responses to new situations is vital for adaptive behavior. We provide a circuit-level account of generalization in the electrosensory lobe (ELL) of weakly electric mormyrid fish. Much is already known in this system about a form of learning in which motor corollary discharge sign...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.44032

    authors: Dempsey C,Abbott LF,Sawtell NB

    更新日期:2019-03-14 00:00:00

  • Deficit of mitogen-activated protein kinase phosphatase 1 (DUSP1) accelerates progressive hearing loss.

    abstract::Mitogen-activated protein kinases (MAPK) such as p38 and the c-Jun N-terminal kinases (JNKs) are activated during the cellular response to stress signals. Their activity is regulated by the MAPK-phosphatase 1 (DUSP1), a key component of the anti-inflammatory response. Stress kinases are well-described elements of the ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.39159

    authors: Celaya AM,Sánchez-Pérez I,Bermúdez-Muñoz JM,Rodríguez-de la Rosa L,Pintado-Berninches L,Perona R,Murillo-Cuesta S,Varela-Nieto I

    更新日期:2019-04-02 00:00:00

  • Pervasive duplication of tumor suppressors in Afrotherians during the evolution of large bodies and reduced cancer risk.

    abstract::The risk of developing cancer is correlated with body size and lifespan within species. Between species, however, there is no correlation between cancer and either body size or lifespan, indicating that large, long-lived species have evolved enhanced cancer protection mechanisms. Elephants and their relatives (Probosc...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.65041

    authors: Vazquez JM,Lynch VJ

    更新日期:2021-01-29 00:00:00

  • Is it possible to reconstruct an accurate cell lineage using CRISPR recorders?

    abstract::Cell lineages provide the framework for understanding how cell fates are decided during development. Describing cell lineages in most organisms is challenging; even a fruit fly larva has ~50,000 cells and a small mammal has >1 billion cells. Recently, the idea of applying CRISPR to induce mutations during development,...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.40292

    authors: Salvador-Martínez I,Grillo M,Averof M,Telford MJ

    更新日期:2019-01-28 00:00:00

  • Arabidopsis plants perform arithmetic division to prevent starvation at night.

    abstract::Photosynthetic starch reserves that accumulate in Arabidopsis leaves during the day decrease approximately linearly with time at night to support metabolism and growth. We find that the rate of decrease is adjusted to accommodate variation in the time of onset of darkness and starch content, such that reserves last al...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.00669

    authors: Scialdone A,Mugford ST,Feike D,Skeffington A,Borrill P,Graf A,Smith AM,Howard M

    更新日期:2013-06-25 00:00:00

  • Metabolic network rewiring of propionate flux compensates vitamin B12 deficiency in C. elegans.

    abstract::Metabolic network rewiring is the rerouting of metabolism through the use of alternate enzymes to adjust pathway flux and accomplish specific anabolic or catabolic objectives. Here, we report the first characterization of two parallel pathways for the breakdown of the short chain fatty acid propionate in Caenorhabditi...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.17670

    authors: Watson E,Olin-Sandoval V,Hoy MJ,Li CH,Louisse T,Yao V,Mori A,Holdorf AD,Troyanskaya OG,Ralser M,Walhout AJ

    更新日期:2016-07-06 00:00:00