相关文献
eLife文献大全abstract::The characteristics of goal-directed actions tend to resemble those of previously executed actions, but it is unclear whether such effects depend strictly on action history, or also reflect context-dependent processes related to predictive motor planning. Here we manipulated the time available to initiate movements af...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.26713
更新日期:2017-10-23 00:00:00
abstract::Actin has well established functions in cellular morphogenesis. However, it is not well understood how the various actin assemblies in a cell are kept in a dynamic equilibrium, in particular when cells have to respond to acute signals. Here, we characterize a rapid and transient actin reset in response to increased in...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19850
更新日期:2016-12-06 00:00:00
abstract::WW domain tandem-containing proteins such as KIBRA, YAP, and MAGI play critical roles in cell growth and polarity via binding to and positioning target proteins in specific subcellular regions. An immense disparity exists between promiscuity of WW domain-mediated target bindings and specific roles of WW domain protein...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49439
更新日期:2019-09-05 00:00:00
abstract::Heterogeneity broadly exists in various cell types both during development and at homeostasis. Investigating heterogeneity is crucial for comprehensively understanding the complexity of ontogeny, dynamics, and function of specific cell types. Traditional bulk-labeling techniques are incompetent to dissect heterogeneit...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52024
更新日期:2020-01-06 00:00:00
abstract::Although pain is a common symptom of various diseases and disorders, its contribution to disease pathogenesis is not well understood. Here we show using murine experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis (MS), that pain induces EAE relapse. Mechanistic analysis showed that pain indu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.08733
更新日期:2015-08-11 00:00:00
abstract::Goal-directed behavior involves distributed neuronal circuits in the mammalian brain, including diverse regions of neocortex. However, the cellular basis of long-range cortico-cortical signaling during goal-directed behavior is poorly understood. Here, we recorded membrane potential of excitatory layer 2/3 pyramidal n...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15798
更新日期:2016-06-21 00:00:00
abstract::Ribosomes are universally important in biology and their production is dysregulated by developmental disorders, cancer, and virus infection. Although presumed required for protein synthesis, how ribosome biogenesis impacts virus reproduction and cell-intrinsic immune responses remains untested. Surprisingly, we find t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49551
更新日期:2019-12-16 00:00:00
abstract::Mitochondrial division, essential for survival in mammals, is enhanced by an inter-organellar process involving ER tubules encircling and constricting mitochondria. The force for constriction is thought to involve actin polymerization by the ER-anchored isoform of the formin protein inverted formin 2 (INF2). Unknown i...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.08828
更新日期:2015-08-25 00:00:00
abstract::We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown. Here we show E. faecium has unique peptidoglycan comp...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45343
更新日期:2019-04-10 00:00:00
abstract::SUMO1-conjugation of proteins at neuronal synapses is considered to be a major post-translational regulatory process in nerve cell and synapse function, but the published evidence for SUMO1-conjugation at synapses is contradictory. We employed multiple genetic mouse models for stringently controlled biochemical and im...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.26338
更新日期:2017-06-09 00:00:00
abstract::Cytoplasmic dynein plays critical roles within the developing and mature nervous systems, including effecting nuclear migration, and retrograde transport of various cargos. Unsurprisingly, mutations in dynein are causative of various developmental neuropathies and motor neuron diseases. These 'dyneinopathies' define a...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47246
更新日期:2019-07-31 00:00:00
abstract::Cyanobacteria were responsible for the oxygenation of the ancient atmosphere; however, the evolution of this phylum is enigmatic, as relatives have not been characterized. Here we use whole genome reconstruction of human fecal and subsurface aquifer metagenomic samples to obtain complete genomes for members of a new c...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01102
更新日期:2013-10-01 00:00:00
abstract::Although activation/deactivation of specific brain regions has been shown to be predictive of successful memory encoding, the relationship between time-varying large-scale brain networks and fluctuations of memory encoding performance remains unclear. Here, we investigated time-varying functional connectivity patterns...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.32696
更新日期:2018-06-18 00:00:00
abstract::Down syndrome (DS) results in various degrees of cognitive deficits. In DS mouse models, recovery of behavioral and neurophysiological deficits using GABAAR antagonists led to hypothesize an excessive activity of inhibitory circuits in this condition. Nonetheless, whether over-inhibition is present in DS and whether t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58731
更新日期:2020-08-12 00:00:00
abstract::Macrophages are highly plastic cells with critical roles in immunity, cancer, and tissue homeostasis, but how these distinct cellular fates are triggered by environmental cues is poorly understood. To uncover how primary murine macrophages respond to bacterial pathogens, we globally assessed changes in post-translatio...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51461
更新日期:2020-01-17 00:00:00
abstract::Manipulating gene function cell type-specifically is a common experimental goal in Drosophila research and has been central to studies of neural development, circuit computation, and behavior. However, current cell type-specific gene disruption techniques in flies often reduce gene activity incompletely or rely on cel...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22279
更新日期:2017-02-17 00:00:00
abstract::B-cell receptors form ordered clusters to recruit kinases and exclude phosphatases. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.25375
更新日期:2017-03-30 00:00:00
abstract::Pregnancy complications are poorly represented in the archeological record, despite their importance in contemporary and ancient societies. While excavating a Byzantine cemetery in Troy, we discovered calcified abscesses among a woman's remains. Scanning electron microscopy of the tissue revealed 'ghost cells', result...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20983
更新日期:2017-01-10 00:00:00
abstract::The vertebral column or spine assembles around the notochord rod which contains a core made of large vacuolated cells. Each vacuolated cell possesses a single fluid-filled vacuole, and loss or fragmentation of these vacuoles in zebrafish leads to spine kinking. Here, we identified a mutation in the kinase gene dstyk tha...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51221
更新日期:2020-01-29 00:00:00
abstract::Bacillus subtilis can measure the activity of the enzymes that remodel the cell wall to ensure that the levels of activity are 'just right'. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54244
更新日期:2020-01-24 00:00:00
abstract::The phylogenetic placement of the morphologically simple placozoans is crucial to understanding the evolution of complex animal traits. Here, we examine the influence of adding new genomes from placozoans to a large dataset designed to study the deepest splits in the animal phylogeny. Using site-heterogeneous substitu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.36278
更新日期:2018-10-30 00:00:00
abstract::Accurate protein localization is crucial to generate and maintain organization in all cells. Achieving accuracy is challenging, as the molecular signals that dictate a protein's cellular destination are often promiscuous. A salient example is the targeting of an essential class of tail-anchored (TA) proteins, whose so...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21301
更新日期:2016-12-07 00:00:00
abstract::Cell lineages provide the framework for understanding how cell fates are decided during development. Describing cell lineages in most organisms is challenging; even a fruit fly larva has ~50,000 cells and a small mammal has >1 billion cells. Recently, the idea of applying CRISPR to induce mutations during development,...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.40292
更新日期:2019-01-28 00:00:00
abstract::Plasmodium sporozoites, the mosquito-transmitted forms of the malaria parasite, first infect the liver for an initial round of replication before the emergence of pathogenic blood stages. Sporozoites represent attractive targets for antimalarial preventive strategies, yet the mechanisms of parasite entry into hepatocy...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25903
更新日期:2017-05-16 00:00:00
abstract::Selective packaging of HIV-1 genomic RNA (gRNA) requires the presence of a cis-acting RNA element called the 'packaging signal' (Ψ). However, the mechanism by which Ψ promotes selective packaging of the gRNA is not well understood. We used fluorescence correlation spectroscopy and quenching data to monitor the binding...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.27055
更新日期:2017-07-20 00:00:00
abstract::Motor fatigability emerges when demanding tasks are executed over an extended period of time. Here, we used repetitive low-force movements that cause a gradual reduction in movement speed (or 'motor slowing') to study the central component of fatigability in healthy adults. We show that motor slowing is associated wit...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46750
更新日期:2019-09-16 00:00:00
abstract::Spatial and temporal cues are required to specify neuronal diversity, but how these cues are integrated in neural progenitors remains unknown. Drosophila progenitors (neuroblasts) are a good model: they are individually identifiable with relevant spatial and temporal transcription factors known. Here we test whether s...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44036
更新日期:2019-01-29 00:00:00
abstract::Advanced microscopy and labeling techniques reveal that bacteria localize mRNAs within their cells in a similar way to eukaryotes. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.16598
更新日期:2016-05-20 00:00:00
abstract::Intergroup conflict contributes to human discrimination and violence, but persists because individuals make costly contributions to their group's fighting capacity. Yet, how group members effectively coordinate their contributions during intergroup conflict remains poorly understood. Here, we examine the role of oxyto...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.40698
更新日期:2019-01-25 00:00:00
abstract::Long noncoding RNAs (lncRNAs) have been shown to act as important cell biological regulators including cell fate decisions but are often ignored in human genetics. Combining differential lncRNA expression during neuronal lineage induction with copy number variation morbidity maps of a cohort of children with autism sp...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.41770
更新日期:2019-01-10 00:00:00