Long-term antigen exposure irreversibly modifies metabolic requirements for T cell function.

abstract：:Actin dynamics generate forces to deform the membrane and overcome the cell's high turgor pressure during clathrin-mediated endocytosis (CME) in yeast, but precise molecular details are still unresolved. Our previous models predicted that actin filaments of the endocytic meshwork continually polymerize and disassemble...

journal_title：eLife

authors： Lacy MM,Baddeley D,Berro J

更新日期：2019-12-19 00:00:00

abstract：:Vesicle fusion is mediated by an assembly of SNARE proteins between opposing membranes, but it is unknown whether transmembrane domains (TMDs) of SNARE proteins serve mechanistic functions that go beyond passive anchoring of the force-generating SNAREpin to the fusing membranes. Here, we show that conformational flexi...

journal_title：eLife

authors： Dhara M,Yarzagaray A,Makke M,Schindeldecker B,Schwarz Y,Shaaban A,Sharma S,Böckmann RA,Lindau M,Mohrmann R,Bruns D

更新日期：2016-06-25 00:00:00

abstract：:The collapse of iconic, keystone populations of sockeye (Oncorhynchus nerka) and Chinook (Oncorhynchus tshawytscha) salmon in the Northeast Pacific is of great concern. It is thought that infectious disease may contribute to declines, but little is known about viruses endemic to Pacific salmon. Metatranscriptomic sequ...

journal_title：eLife

authors： Mordecai GJ,Miller KM,Di Cicco E,Schulze AD,Kaukinen KH,Ming TJ,Li S,Tabata A,Teffer A,Patterson DA,Ferguson HW,Suttle CA

更新日期：2019-09-03 00:00:00

abstract：:Our knowledge about the repertoire of ribosomal RNA modifications and the enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 is responsible for depositing m5C at position C2381 on the 26S rRNA in Caenorhabditis elegans. Here, we show that NSUN-1 is writing the second k...

journal_title：eLife

authors： Heissenberger C,Rollins JA,Krammer TL,Nagelreiter F,Stocker I,Wacheul L,Shpylovyi A,Tav K,Snow S,Grillari J,Rogers AN,Lafontaine DLJ,Schosserer M

更新日期：2020-12-08 00:00:00

abstract：:MyD88 is the main adaptor molecule for TLR and IL-1R family members. Here, we demonstrated that T-cell intrinsic MyD88 signaling is required for proliferation, protection from apoptosis and expression of activation/memory genes during infection with the intracellular parasite Trypanosoma cruzi, as evidenced by transcr...

journal_title：eLife

authors： Oliveira AC,Gomes-Neto JF,Barbosa CD,Granato A,Reis BS,Santos BM,Fucs R,Canto FB,Nakaya HI,Nóbrega A,Bellio M

更新日期：2017-09-12 00:00:00

abstract：:Planar cell polarity (PCP) requires the asymmetric sorting of distinct signaling receptors to distal and proximal surfaces of polarized epithelial cells. We have examined the transport of one PCP signaling protein, Vangl2, from the trans Golgi network (TGN) in mammalian cells. Using siRNA knockdown experiments, we fin...

journal_title：eLife

authors： Guo Y,Zanetti G,Schekman R

更新日期：2013-01-08 00:00:00

abstract：:AKT is implicated in neurological disorders. AKT has three isoforms, AKT1/AKT2/AKT3, with brain cell type-specific expression that may differentially influence behavior. Therefore, we examined single Akt isoform, conditional brain-specific Akt1, and double Akt1/3 mutant mice in behaviors relevant to neuropsychiatric d...

journal_title：eLife

authors： Wong H,Levenga J,LaPlante L,Keller B,Cooper-Sansone A,Borski C,Milstead R,Ehringer M,Hoeffer C

更新日期：2020-12-16 00:00:00

abstract：:Sparse, sequential patterns of neural activity have been observed in numerous brain areas during timekeeping and motor sequence tasks. Inspired by such observations, we construct a model of the striatum, an all-inhibitory circuit where sequential activity patterns are prominent, addressing the following key challenges...

journal_title：eLife

authors： Murray JM,Escola GS

更新日期：2017-05-08 00:00:00

abstract：:Toothed whales use sonar to detect, locate, and track prey. They adjust emitted sound intensity, auditory sensitivity and click rate to target range, and terminate prey pursuits with high-repetition-rate, low-intensity buzzes. However, their narrow acoustic field of view (FOV) is considered stable throughout target ap...

journal_title：eLife

authors： Wisniewska DM,Ratcliffe JM,Beedholm K,Christensen CB,Johnson M,Koblitz JC,Wahlberg M,Madsen PT

更新日期：2015-03-20 00:00:00

abstract：:Myelin destruction is followed by resident glia activation and mobilization of endogenous progenitors (OPC) which participate in myelin repair. Here we show that in response to demyelination, mature oligodendrocytes (OLG) bordering the lesion express Ndst1, a key enzyme for heparan sulfates (HS) synthesis. Ndst1+ OLG ...

journal_title：eLife

authors： Macchi M,Magalon K,Zimmer C,Peeva E,El Waly B,Brousse B,Jaekel S,Grobe K,Kiefer F,Williams A,Cayre M,Durbec P

更新日期：2020-06-09 00:00:00

abstract：:The spliceosome is a complex machine composed of small nuclear ribonucleoproteins (snRNPs) and accessory proteins that excises introns from pre-mRNAs. After assembly the spliceosome is activated for catalysis by rearrangement of subunits to form an active site. How this rearrangement is coordinated is not well-underst...

journal_title：eLife

authors： Hoskins AA,Rodgers ML,Friedman LJ,Gelles J,Moore MJ

更新日期：2016-05-31 00:00:00

abstract：:The medial subnucleus of the amygdala (MeA) plays a central role in processing sensory cues required for innate behaviors. However, whether there is a link between developmental programs and the emergence of inborn behaviors remains unknown. Our previous studies revealed that the telencephalic preoptic area (POA) embr...

journal_title：eLife

authors： Lischinsky JE,Sokolowski K,Li P,Esumi S,Kamal Y,Goodrich M,Oboti L,Hammond TR,Krishnamoorthy M,Feldman D,Huntsman M,Liu J,Corbin JG

更新日期：2017-03-13 00:00:00

abstract：:Behavior relies on the ability of sensory systems to infer properties of the environment from incoming stimuli. The accuracy of inference depends on the fidelity with which behaviorally relevant properties of stimuli are encoded in neural responses. High-fidelity encodings can be metabolically costly, but low-fidelity...

journal_title：eLife

authors： Młynarski WF,Hermundstad AM

更新日期：2018-07-10 00:00:00

abstract：:Drug treatment of 3D cancer spheroids more accurately reflects in vivo therapeutic responses compared to adherent culture studies. In EGFR-mutated lung adenocarcinoma, EGFR-TKIs show enhanced efficacy in spheroid cultures. Simultaneous inhibition of multiple parallel RTKs further enhances EGFR-TKI effectiveness. We sh...

journal_title：eLife

authors： Theard PL,Sheffels E,Sealover NE,Linke AJ,Pratico DJ,Kortum RL

更新日期：2020-09-08 00:00:00

abstract：:Individual recognition (IR) is essential for maintaining various social interactions in a group, and face recognition is one of the most specialised cognitive abilities in IR. We used both a mating preference system and an electric shock conditioning experiment to test IR ability in medaka, and found that signals near...

journal_title：eLife

authors： Wang MY,Takeuchi H

更新日期：2017-07-11 00:00:00

abstract：:The COVID-19 pandemic is a harsh reminder of the fact that, whether in a single human host or a wave of infection across continents, viral dynamics is often a story about the numbers. In this article we provide a one-stop, curated graphical source for the key numbers (based mostly on the peer-reviewed literature) abou...

journal_title：eLife

authors： Bar-On YM,Flamholz A,Phillips R,Milo R

更新日期：2020-04-02 00:00:00

abstract：:The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the...

journal_title：eLife

authors： Phelps M,Coss C,Wang H,Cook M,Reproducibility Project: Cancer Biology.,Reproducibility Project Cancer Biology.

更新日期：2016-03-01 00:00:00

abstract：:Despite their importance in sexual differentiation and reproduction, Y chromosome genes are rarely described because they reside in repeat-rich regions that are difficult to study. Here, we show that Guy1, a unique Y chromosome gene of a major urban malaria mosquito Anopheles stephensi, confers 100% female lethality w...

journal_title：eLife

authors： Criscione F,Qi Y,Tu Z

更新日期：2016-09-20 00:00:00

abstract：:How producers of public goods persist in microbial communities is a major question in evolutionary biology. Cooperation is evolutionarily unstable, since cheating strains can reproduce quicker and take over. Spatial structure has been shown to be a robust mechanism for the evolution of cooperation. Here we study how s...

journal_title：eLife

authors： Uppal G,Vural DC

更新日期：2018-05-22 00:00:00

abstract：:Certain neuron types fire spontaneously at high rates, an ability that is crucial for their function in brain circuits. The spontaneously active GABAergic neurons of the substantia nigra pars reticulata (SNr), a major output of the basal ganglia, provide tonic inhibition of downstream brain areas. A depolarizing 'leak...

journal_title：eLife

authors： Lutas A,Lahmann C,Soumillon M,Yellen G

更新日期：2016-05-13 00:00:00

abstract：:Positive-strand RNA viruses, the largest genetic class of viruses, include numerous important pathogens such as Zika virus. These viruses replicate their RNA genomes in novel, membrane-bounded mini-organelles, but the organization of viral proteins and RNAs in these compartments has been largely unknown. We used cryo-...

journal_title：eLife

authors： Ertel KJ,Benefield D,Castaño-Diez D,Pennington JG,Horswill M,den Boon JA,Otegui MS,Ahlquist P

更新日期：2017-06-27 00:00:00

abstract：:The factors that govern assembly of the gut microbiota are insufficiently understood. Here, we test the hypothesis that inter-individual microbiota variation can arise solely from differences in the order and timing by which the gut is colonized early in life. Experiments in which mice were inoculated in sequence eith...

journal_title：eLife

authors： Martínez I,Maldonado-Gomez MX,Gomes-Neto JC,Kittana H,Ding H,Schmaltz R,Joglekar P,Cardona RJ,Marsteller NL,Kembel SW,Benson AK,Peterson DA,Ramer-Tait AE,Walter J

更新日期：2018-09-18 00:00:00

abstract：:The neural correlates of consciousness are typically sought by comparing the overall brain responses to perceived and unperceived stimuli. However, this comparison may be contaminated by non-specific attention, alerting, performance, and reporting confounds. Here, we pursue a novel approach, tracking the neuronal codi...

journal_title：eLife

authors： Salti M,Monto S,Charles L,King JR,Parkkonen L,Dehaene S

更新日期：2015-05-21 00:00:00

abstract：:Myelin, made by oligodendrocytes, is essential for rapid information transfer in the central nervous system. Oligodendrocyte precursors (OPs) receive glutamatergic synaptic input from axons but how this affects their development is unclear. Murine OPs in white matter express AMPA receptor (AMPAR) subunits GluA2, GluA3...

journal_title：eLife

authors： Kougioumtzidou E,Shimizu T,Hamilton NB,Tohyama K,Sprengel R,Monyer H,Attwell D,Richardson WD

更新日期：2017-06-13 00:00:00

abstract：:Cognitive control proactively configures information processing to suit expected task demands. Predictions of forthcoming demand can be driven by explicit external cues or be generated internally, based on past experience (cognitive history). However, it is not known whether and how the brain reconciles these two sour...

journal_title：eLife

authors： Jiang J,Wagner AD,Egner T

更新日期：2018-08-16 00:00:00

abstract：:The voltage-gated K+ channel Kv2.1 serves a major structural role in the soma and proximal dendrites of mammalian brain neurons, tethering the plasma membrane (PM) to endoplasmic reticulum (ER). Although Kv2.1 clustering at neuronal ER-PM junctions (EPJs) is tightly regulated and highly conserved, its function remains...

journal_title：eLife

authors： Vierra NC,Kirmiz M,van der List D,Santana LF,Trimmer JS

更新日期：2019-10-30 00:00:00

abstract：: ...

journal_title：eLife

authors： Zhou FY,Ruiz-Puig C,Owen RP,White MJ,Rittscher J,Lu X

更新日期：2019-07-02 00:00:00

abstract：:The Escherichia coli DNA replication machinery has been used as a road map to uncover design rules that enable DNA duplication with high efficiency and fidelity. Although the enzymatic activities of the replicative DNA Pol III are well understood, its dynamics within the replisome are not. Here, we test the accepted v...

journal_title：eLife

authors： Lewis JS,Spenkelink LM,Jergic S,Wood EA,Monachino E,Horan NP,Duderstadt KE,Cox MM,Robinson A,Dixon NE,van Oijen AM

更新日期：2017-04-22 00:00:00

abstract：:Whether neurons encode information through their spike rates, their activity times or both is an ongoing debate in systems neuroscience. Here, we tested whether humans can discriminate between a pair of temporal odor mixtures (TOMs) composed of the same two components delivered in rapid succession in either one tempor...

journal_title：eLife

authors： Perl O,Nahum N,Belelovsky K,Haddad R

更新日期：2020-02-07 00:00:00

abstract：:Evolutionary changes in transcription networks are an important source of diversity across species, yet the quantitative consequences of network evolution have rarely been studied. Here we consider the transcriptional 'rewiring' of the three GAL genes that encode the enzymes needed for cells to convert galactose to gl...

journal_title：eLife

authors： Dalal CK,Zuleta IA,Mitchell KF,Andes DR,El-Samad H,Johnson AD

更新日期：2016-09-10 00:00:00