Dissection of specific binding of HIV-1 Gag to the 'packaging signal' in viral RNA.

Abstract:

:Selective packaging of HIV-1 genomic RNA (gRNA) requires the presence of a cis-acting RNA element called the 'packaging signal' (Ψ). However, the mechanism by which Ψ promotes selective packaging of the gRNA is not well understood. We used fluorescence correlation spectroscopy and quenching data to monitor the binding of recombinant HIV-1 Gag protein to Cy5-tagged 190-base RNAs. At physiological ionic strength, Gag binds with very similar, nanomolar affinities to both Ψ-containing and control RNAs. We challenged these interactions by adding excess competing tRNA; introducing mutations in Gag; or raising the ionic strength. These modifications all revealed high specificity for Ψ. This specificity is evidently obscured in physiological salt by non-specific, predominantly electrostatic interactions. This nonspecific activity was attenuated by mutations in the MA, CA, and NC domains, including CA mutations disrupting Gag-Gag interaction. We propose that gRNA is selectively packaged because binding to Ψ nucleates virion assembly with particular efficiency.

journal_name

Elife

journal_title

eLife

authors

Comas-Garcia M,Datta SA,Baker L,Varma R,Gudla PR,Rein A

doi

10.7554/eLife.27055

subject

Has Abstract

pub_date

2017-07-20 00:00:00

issn

2050-084X

journal_volume

6

pub_type

杂志文章

相关文献

eLife文献大全
  • Diminished responses to bodily threat and blunted interoception in suicide attempters.

    abstract::Psychological theories of suicide suggest that certain traits may reduce aversion to physical threat and increase the probability of transitioning from suicidal ideation to action. Here, we investigated whether blunted sensitivity to bodily signals is associated with suicidal action by comparing individuals with a his...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.51593

    authors: DeVille DC,Kuplicki R,Stewart JL,Tulsa 1000 Investigators.,Paulus MP,Khalsa SS

    更新日期:2020-04-07 00:00:00

  • RNA-dependent stabilization of SUV39H1 at constitutive heterochromatin.

    abstract::Heterochromatin formed by the SUV39 histone methyltransferases represses transcription from repetitive DNA sequences and ensures genomic stability. How SUV39 enzymes localize to their target genomic loci remains unclear. Here, we demonstrate that chromatin-associated RNA contributes to the stable association of SUV39H...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.25299

    authors: Johnson WL,Yewdell WT,Bell JC,McNulty SM,Duda Z,O'Neill RJ,Sullivan BA,Straight AF

    更新日期:2017-08-01 00:00:00

  • The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment.

    abstract::The palmitoyl acyltransferase (PAT) ZDHHC14 is highly expressed in the hippocampus and is the only PAT predicted to bind Type-I PDZ domain-containing proteins. However, ZDHHC14's neuronal roles are unknown. Here, we identify the PDZ domain-containing Membrane-associated Guanylate Kinase (MaGUK) PSD93 as a direct ZDHHC...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.56058

    authors: Sanders SS,Hernandez LM,Soh H,Karnam S,Walikonis RS,Tzingounis AV,Thomas GM

    更新日期:2020-11-13 00:00:00

  • Many si/shRNAs can kill cancer cells by targeting multiple survival genes through an off-target mechanism.

    abstract::Over 80% of multiple-tested siRNAs and shRNAs targeting CD95 or CD95 ligand (CD95L) induce a form of cell death characterized by simultaneous activation of multiple cell death pathways preferentially killing transformed and cancer stem cells. We now show these si/shRNAs kill cancer cells through canonical RNAi by targ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.29702

    authors: Putzbach W,Gao QQ,Patel M,van Dongen S,Haluck-Kangas A,Sarshad AA,Bartom ET,Kim KA,Scholtens DM,Hafner M,Zhao JC,Murmann AE,Peter ME

    更新日期:2017-10-24 00:00:00

  • Exchange of water for sterol underlies sterol egress from a StARkin domain.

    abstract::Previously we identified Lam/GramD1 proteins, a family of endoplasmic reticulum membrane proteins with sterol-binding StARkin domains that are implicated in intracellular sterol homeostasis. Here, we show how these proteins exchange sterol molecules with membranes. An aperture at one end of the StARkin domain enables ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.53444

    authors: Khelashvili G,Chauhan N,Pandey K,Eliezer D,Menon AK

    更新日期:2019-12-04 00:00:00

  • Nuclear genetic regulation of the human mitochondrial transcriptome.

    abstract::Mitochondria play important roles in cellular processes and disease, yet little is known about how the transcriptional regime of the mitochondrial genome varies across individuals and tissues. By analyzing >11,000 RNA-sequencing libraries across 36 tissue/cell types, we find considerable variation in mitochondrial-enc...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.41927

    authors: Ali AT,Boehme L,Carbajosa G,Seitan VC,Small KS,Hodgkinson A

    更新日期:2019-02-18 00:00:00

  • Regulation of the Drosophila ID protein Extra macrochaetae by proneural dimerization partners.

    abstract::Proneural bHLH proteins are transcriptional regulators of neural fate specification. Extra macrochaetae (Emc) forms inactive heterodimers with both proneural bHLH proteins and their bHLH partners (represented in Drosophila by Daughterless). It is generally thought that varying levels of Emc define a prepattern that de...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.33967

    authors: Li K,Baker NE

    更新日期:2018-04-24 00:00:00

  • Cryo-EM structure of a late pre-40S ribosomal subunit from Saccharomyces cerevisiae.

    abstract::Mechanistic understanding of eukaryotic ribosome formation requires a detailed structural knowledge of the numerous assembly intermediates, generated along a complex pathway. Here, we present the structure of a late pre-40S particle at 3.6 Å resolution, revealing in molecular detail how assembly factors regulate the t...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.30189

    authors: Heuer A,Thomson E,Schmidt C,Berninghausen O,Becker T,Hurt E,Beckmann R

    更新日期:2017-11-20 00:00:00

  • A conserved function for pericentromeric satellite DNA.

    abstract::A universal and unquestioned characteristic of eukaryotic cells is that the genome is divided into multiple chromosomes and encapsulated in a single nucleus. However, the underlying mechanism to ensure such a configuration is unknown. Here, we provide evidence that pericentromeric satellite DNA, which is often regarde...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.34122

    authors: Jagannathan M,Cummings R,Yamashita YM

    更新日期:2018-03-26 00:00:00

  • Molecular basis for dyneinopathies reveals insight into dynein regulation and dysfunction.

    abstract::Cytoplasmic dynein plays critical roles within the developing and mature nervous systems, including effecting nuclear migration, and retrograde transport of various cargos. Unsurprisingly, mutations in dynein are causative of various developmental neuropathies and motor neuron diseases. These 'dyneinopathies' define a...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.47246

    authors: Marzo MG,Griswold JM,Ruff KM,Buchmeier RE,Fees CP,Markus SM

    更新日期:2019-07-31 00:00:00

  • Recalibrating timing behavior via expected covariance between temporal cues.

    abstract::Individuals must predict future events to proactively guide their behavior. Predicting when events will occur is a critical component of these expectations. Temporal expectations are often generated based on individual cue-duration relationships. However, the durations associated with different environmental cues will...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.38790

    authors: De Corte BJ,Della Valle RR,Matell MS

    更新日期:2018-11-02 00:00:00

  • SynEM, automated synapse detection for connectomics.

    abstract::Nerve tissue contains a high density of chemical synapses, about 1 per µm3 in the mammalian cerebral cortex. Thus, even for small blocks of nerve tissue, dense connectomic mapping requires the identification of millions to billions of synapses. While the focus of connectomic data analysis has been on neurite reconstru...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.26414

    authors: Staffler B,Berning M,Boergens KM,Gour A,Smagt PV,Helmstaedter M

    更新日期:2017-07-14 00:00:00

  • Impact of seasonal variations in Plasmodium falciparum malaria transmission on the surveillance of pfhrp2 gene deletions.

    abstract::Ten countries have reported pfhrp2/pfhrp3 gene deletions since the first observation of pfhrp2-deleted parasites in 2012. In a previous study (Watson et al., 2017), we characterised the drivers selecting for pfhrp2/3 deletions and mapped the regions in Africa with the greatest selection pressure. In February 2018, the...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.40339

    authors: Watson OJ,Verity R,Ghani AC,Garske T,Cunningham J,Tshefu A,Mwandagalirwa MK,Meshnick SR,Parr JB,Slater HC

    更新日期:2019-05-02 00:00:00

  • CRISPR/Cas9 and active genetics-based trans-species replacement of the endogenous Drosophila kni-L2 CRM reveals unexpected complexity.

    abstract::The knirps (kni) locus encodes transcription factors required for induction of the L2 wing vein in Drosophila. Here, we employ diverse CRISPR/Cas9 genome editing tools to generate a series of targeted lesions within the endogenous cis-regulatory module (CRM) required for kni expression in the L2 vein primordium. Pheno...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.30281

    authors: Xu XS,Gantz VM,Siomava N,Bier E

    更新日期:2017-12-23 00:00:00

  • A survey-based analysis of the academic job market.

    abstract::Many postdoctoral researchers apply for faculty positions knowing relatively little about the hiring process or what is needed to secure a job offer. To address this lack of knowledge about the hiring process we conducted a survey of applicants for faculty positions: the survey ran between May 2018 and May 2019, and r...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.54097

    authors: Fernandes JD,Sarabipour S,Smith CT,Niemi NM,Jadavji NM,Kozik AJ,Holehouse AS,Pejaver V,Symmons O,Bisson Filho AW,Haage A

    更新日期:2020-06-12 00:00:00

  • Alleviation of neuronal energy deficiency by mTOR inhibition as a treatment for mitochondria-related neurodegeneration.

    abstract::mTOR inhibition is beneficial in neurodegenerative disease models and its effects are often attributable to the modulation of autophagy and anti-apoptosis. Here, we report a neglected but important bioenergetic effect of mTOR inhibition in neurons. mTOR inhibition by rapamycin significantly preserves neuronal ATP leve...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.13378

    authors: Zheng X,Boyer L,Jin M,Kim Y,Fan W,Bardy C,Berggren T,Evans RM,Gage FH,Hunter T

    更新日期:2016-03-23 00:00:00

  • The Caenorhabditis elegans microtubule minus-end binding homolog PTRN-1 stabilizes synapses and neurites.

    abstract::Microtubule dynamics facilitate neurite growth and establish morphology, but the role of minus-end binding proteins in these processes is largely unexplored. CAMSAP homologs associate with microtubule minus-ends, and are important for the stability of epithelial cell adhesions. In this study, we report morphological d...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.01637

    authors: Marcette JD,Chen JJ,Nonet ML

    更新日期:2014-02-25 00:00:00

  • Sex-specific effects of cooperative breeding and colonial nesting on prosociality in corvids.

    abstract::The investigation of prosocial behavior is of particular interest from an evolutionary perspective. Comparisons of prosociality across non-human animal species have, however, so far largely focused on primates, and their interpretation is hampered by the diversity of paradigms and procedures used. Here, we present the...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.58139

    authors: Horn L,Bugnyar T,Griesser M,Hengl M,Izawa EI,Oortwijn T,Rössler C,Scheer C,Schiestl M,Suyama M,Taylor AH,Vanhooland LC,von Bayern AM,Zürcher Y,Massen JJ

    更新日期:2020-10-20 00:00:00

  • Rescue of cognitive function following fractionated brain irradiation in a novel preclinical glioma model.

    abstract::More than half of long-term brain tumor survivors develop irreversible cognitive decline that severely affect their quality of life. However, there is no pre-clinical model that allows long-term assessment of cognition, and there is no treatment which ameliorates cognitive deficits in patients. Here, we report a novel...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.38865

    authors: Feng X,Liu S,Chen D,Rosi S,Gupta N

    更新日期:2018-11-13 00:00:00

  • Phylogenetic divergence of cell biological features.

    abstract::Most cellular features have a range of states, but understanding the mechanisms responsible for interspecific divergence is a challenge for evolutionary cell biology. Models are developed for the distribution of mean phenotypes likely to evolve under the joint forces of mutation and genetic drift in the face of consta...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.34820

    authors: Lynch M

    更新日期:2018-06-21 00:00:00

  • An essential cell-autonomous role for hepcidin in cardiac iron homeostasis.

    abstract::Hepcidin is the master regulator of systemic iron homeostasis. Derived primarily from the liver, it inhibits the iron exporter ferroportin in the gut and spleen, the sites of iron absorption and recycling respectively. Recently, we demonstrated that ferroportin is also found in cardiomyocytes, and that its cardiac-spe...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.19804

    authors: Lakhal-Littleton S,Wolna M,Chung YJ,Christian HC,Heather LC,Brescia M,Ball V,Diaz R,Santos A,Biggs D,Clarke K,Davies B,Robbins PA

    更新日期:2016-11-29 00:00:00

  • Epimutations are associated with CHROMOMETHYLASE 3-induced de novo DNA methylation.

    abstract::In many plant species, a subset of transcribed genes are characterized by strictly CG-context DNA methylation, referred to as gene body methylation (gbM). The mechanisms that establish gbM are unclear, yet flowering plant species naturally without gbM lack the DNA methyltransferase, CMT3, which maintains CHG (H = A, C...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.47891

    authors: Wendte JM,Zhang Y,Ji L,Shi X,Hazarika RR,Shahryary Y,Johannes F,Schmitz RJ

    更新日期:2019-07-29 00:00:00

  • Doa10 is a membrane protein retrotranslocase in ER-associated protein degradation.

    abstract::In endoplasmic reticulum-associated protein degradation (ERAD), membrane proteins are ubiquitinated, extracted from the membrane, and degraded by the proteasome. The cytosolic ATPase Cdc48 drives extraction by pulling on polyubiquitinated substrates. How hydrophobic transmembrane (TM) segments are moved from the phosp...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.56945

    authors: Schmidt CC,Vasic V,Stein A

    更新日期:2020-06-26 00:00:00

  • Dynamic control of hippocampal spatial coding resolution by local visual cues.

    abstract::The ability to flexibly navigate an environment relies on a hippocampal-dependent cognitive map. External space can be internally mapped at different spatial resolutions. However, whether hippocampal spatial coding resolution can rapidly adapt to local features of an environment remains unclear. To explore this possib...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.44487

    authors: Bourboulou R,Marti G,Michon FX,El Feghaly E,Nouguier M,Robbe D,Koenig J,Epsztein J

    更新日期:2019-03-01 00:00:00

  • NMDA spikes mediate amplification of inputs in the rat piriform cortex.

    abstract::The piriform cortex (PCx) receives direct input from the olfactory bulb (OB) and is the brain's main station for odor recognition and memory. The transformation of the odor code from OB to PCx is profound: mitral and tufted cells in olfactory glomeruli respond to individual odorant molecules, whereas pyramidal neurons...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.38446

    authors: Kumar A,Schiff O,Barkai E,Mel BW,Poleg-Polsky A,Schiller J

    更新日期:2018-12-21 00:00:00

  • Effectiveness of traveller screening for emerging pathogens is shaped by epidemiology and natural history of infection.

    abstract::During outbreaks of high-consequence pathogens, airport screening programs have been deployed to curtail geographic spread of infection. The effectiveness of screening depends on several factors, including pathogen natural history and epidemiology, human behavior, and characteristics of the source epidemic. We develop...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.05564

    authors: Gostic KM,Kucharski AJ,Lloyd-Smith JO

    更新日期:2015-02-19 00:00:00

  • Cortical bone maturation in mice requires SOCS3 suppression of gp130/STAT3 signalling in osteocytes.

    abstract::Bone strength is determined by its dense cortical shell, generated by unknown mechanisms. Here we use the Dmp1Cre:Socs3f/f mouse, with delayed cortical bone consolidation, to characterise cortical maturation and identify control signals. We show that cortical maturation requires a reduction in cortical porosity, and a...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.56666

    authors: Walker EC,Truong K,McGregor NE,Poulton IJ,Isojima T,Gooi JH,Martin TJ,Sims NA

    更新日期:2020-05-27 00:00:00

  • Shigella entry unveils a calcium/calpain-dependent mechanism for inhibiting sumoylation.

    abstract::Disruption of the sumoylation/desumoylation equilibrium is associated with several disease states such as cancer and infections, however the mechanisms regulating the global SUMO balance remain poorly defined. Here, we show that infection by Shigella flexneri, the causative agent of human bacillary dysentery, switches...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.27444

    authors: Lapaquette P,Fritah S,Lhocine N,Andrieux A,Nigro G,Mounier J,Sansonetti P,Dejean A

    更新日期:2017-12-12 00:00:00

  • Architecture of the chromatin remodeler RSC and insights into its nucleosome engagement.

    abstract::Eukaryotic DNA is packaged into nucleosome arrays, which are repositioned by chromatin remodeling complexes to control DNA accessibility. The Saccharomyces cerevisiae RSC (Remodeling the Structure of Chromatin) complex, a member of the SWI/SNF chromatin remodeler family, plays critical roles in genome maintenance, tra...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.54449

    authors: Patel AB,Moore CM,Greber BJ,Luo J,Zukin SA,Ranish J,Nogales E

    更新日期:2019-12-30 00:00:00

  • Lipid polarity gradient formed by ω-hydroxy lipids in tear film prevents dry eye disease.

    abstract::Meibum lipids form a lipid layer on the outermost side of the tear film and function to prevent water evaporation and reduce surface tension. (O-Acyl)-ω-hydroxy fatty acids (OAHFAs), a subclass of these lipids, are thought to be involved in connecting the lipid and aqueous layers in tears, although their actual functi...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.53582

    authors: Miyamoto M,Sassa T,Sawai M,Kihara A

    更新日期:2020-04-07 00:00:00