Abstract:
:Centrioles are microtubule-based organelles crucial for cell division, sensing and motility. In Caenorhabditis elegans, the onset of centriole formation requires notably the proteins SAS-5 and SAS-6, which have functional equivalents across eukaryotic evolution. Whereas the molecular architecture of SAS-6 and its role in initiating centriole formation are well understood, the mechanisms by which SAS-5 and its relatives function is unclear. Here, we combine biophysical and structural analysis to uncover the architecture of SAS-5 and examine its functional implications in vivo. Our work reveals that two distinct self-associating domains are necessary to form higher-order oligomers of SAS-5: a trimeric coiled coil and a novel globular dimeric Implico domain. Disruption of either domain leads to centriole duplication failure in worm embryos, indicating that large SAS-5 assemblies are necessary for function in vivo.
journal_name
Elifejournal_title
eLifeauthors
Rogala KB,Dynes NJ,Hatzopoulos GN,Yan J,Pong SK,Robinson CV,Deane CM,Gönczy P,Vakonakis Idoi
10.7554/eLife.07410subject
Has Abstractpub_date
2015-05-29 00:00:00pages
e07410issn
2050-084Xjournal_volume
4pub_type
杂志文章相关文献
eLife文献大全abstract::Disruption of the sumoylation/desumoylation equilibrium is associated with several disease states such as cancer and infections, however the mechanisms regulating the global SUMO balance remain poorly defined. Here, we show that infection by Shigella flexneri, the causative agent of human bacillary dysentery, switches...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.27444
更新日期:2017-12-12 00:00:00
abstract::The expansion of bacterial swarms and the spreading of biofilms can be described by a unified biophysical theory that involves both active and passive processes. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47019
更新日期:2019-04-30 00:00:00
abstract::During outbreaks of high-consequence pathogens, airport screening programs have been deployed to curtail geographic spread of infection. The effectiveness of screening depends on several factors, including pathogen natural history and epidemiology, human behavior, and characteristics of the source epidemic. We develop...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.05564
更新日期:2015-02-19 00:00:00
abstract::Positive-strand RNA viruses, the largest genetic class of viruses, include numerous important pathogens such as Zika virus. These viruses replicate their RNA genomes in novel, membrane-bounded mini-organelles, but the organization of viral proteins and RNAs in these compartments has been largely unknown. We used cryo-...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25940
更新日期:2017-06-27 00:00:00
abstract::Heterochromatin exerts a heritable form of eukaryotic gene repression and contributes to chromosome segregation fidelity and genome stability. However, to date there has been no quantitative evaluation of the stability of heterochromatic gene repression. We designed a genetic strategy to capture transient losses of ge...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.05007
更新日期:2015-01-12 00:00:00
abstract::How mammalian tissues maintain their architecture and tissue-specificity is poorly understood. Previously, we documented both the indispensable role of the extracellular matrix (ECM) protein, laminin-111 (LN1), in the formation of normal breast acini, and the phenotypic reversion of cancer cells to acini-like structur...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.26148
更新日期:2018-03-21 00:00:00
abstract::From bacteria to mammals, different phospholipid species are segregated between the inner and outer leaflets of the plasma membrane by ATP-dependent lipid transporters. Disruption of this asymmetry by ATP-independent phospholipid scrambling is important in cellular signaling, but its mechanism remains incompletely und...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.28671
更新日期:2017-09-16 00:00:00
abstract::Secondary metabolites have a great potential as pharmaceuticals, but there are only a few examples where regulation of gene cluster expression has been correlated with ecological and physiological relevance for the producer. Here, signals, mediators, and biological effects of terrein production were studied in the fun...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.07861
更新日期:2015-07-14 00:00:00
abstract::Autophagy has been linked to longevity in many species, but the underlying mechanisms are unclear. Using a GFP-tagged and a new tandem-tagged Atg8/LGG-1 reporter, we quantified autophagic vesicles and performed autophagic flux assays in multiple tissues of wild-type Caenorhabditis elegans and long-lived daf-2/insulin/...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18459
更新日期:2017-07-04 00:00:00
abstract::In most animals, the brain controls the body via a set of descending neurons (DNs) that traverse the neck. DN activity activates, maintains or modulates locomotion and other behaviors. Individual DNs have been well-studied in species from insects to primates, but little is known about overall connectivity patterns acr...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.34272
更新日期:2018-06-26 00:00:00
abstract::The connection between gene loss and the functional adaptation of retained proteins is still poorly understood. We apply phylogenomics and metabolic modeling to detect bacterial species that are evolving by gene loss, with the finding that Actinomycetaceae genomes from human cavities are undergoing sizable reductions,...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22679
更新日期:2017-03-31 00:00:00
abstract::Interaction between disease-microbiome associations and ageing has not been explored in detail. Here, using age/region-matched sub-sets, we analysed the gut microbiome differences across five major diseases in a multi-cohort dataset constituting more than 2500 individuals from 20 to 89 years old. We show that disease-...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50240
更新日期:2020-03-11 00:00:00
abstract::Individuals vary in their responses to stroke and trauma, hampering predictions of outcomes. One reason might be that neural circuits contain hidden variability that becomes relevant only when those individuals are challenged by injury. We found that in the mollusc, Tritonia diomedea, subtle differences between animal...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.02598
更新日期:2014-06-11 00:00:00
abstract::To elucidate new functions of sphingosine (Sph), we demonstrate that the spontaneous elevation of intracellular Sph levels via caged Sph leads to a significant and transient calcium release from acidic stores that is independent of sphingosine 1-phosphate, extracellular and ER calcium levels. This photo-induced Sph-dr...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10616
更新日期:2015-11-27 00:00:00
abstract::Microbes are embedded in complex communities where they engage in a wide array of intra- and inter-specific interactions. The extent to which these interactions drive or impede microbiome diversity is not well understood. Historically, two contrasting hypotheses have been suggested to explain how species interactions ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58999
更新日期:2020-11-20 00:00:00
abstract::In many plant species, a subset of transcribed genes are characterized by strictly CG-context DNA methylation, referred to as gene body methylation (gbM). The mechanisms that establish gbM are unclear, yet flowering plant species naturally without gbM lack the DNA methyltransferase, CMT3, which maintains CHG (H = A, C...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47891
更新日期:2019-07-29 00:00:00
abstract::Ribonucleotide reductases (RNRs) convert ribonucleotides into deoxyribonucleotides, a reaction essential for DNA replication and repair. Human RNR requires two subunits for activity, the α subunit contains the active site, and the β subunit houses the radical cofactor. Here, we present a 3.3-Å resolution structure by ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.31502
更新日期:2018-02-20 00:00:00
abstract::The HIF (hypoxia-inducible factor) transcription factor is the master regulator of the metazoan response to chronic hypoxia. In addition to promoting adaptations to low oxygen, HIF drives cytoprotective mechanisms in response to stresses and modulates neural circuit function. How most HIF targets act in the control of...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.36828
更新日期:2018-07-16 00:00:00
abstract::Hox genes are essential regulators of embryonic development. Their step-wise transcriptional activation follows their genomic topology and the various states of activation are subsequently memorized into domains of progressively overlapping gene products. We have analyzed the 3D chromatin organization of Hox clusters ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.02557
更新日期:2014-04-29 00:00:00
abstract::Iron storage proteins are essential for cellular iron homeostasis and redox balance. Ferritin proteins are the major storage units for bioavailable forms of iron. Some organisms lack ferritins, and it is not known how they store iron. Encapsulins, a class of protein-based organelles, have recently been implicated in m...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46070
更新日期:2019-07-08 00:00:00
abstract::Cells deficient in the pro-death Bcl-2 family members Bax and Bak are known to be resistant to apoptotic cell death, and previous we have shown that these two effectors are also needed for mitochondrial-dependent cellular necrosis (Karch et al., 2013). Here we show that mouse embryonic fibroblasts deficient in Bax/Bak...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30543
更新日期:2017-11-17 00:00:00
abstract::The unfolded protein response (UPR) adjusts the cell's protein folding capacity in the endoplasmic reticulum (ER) according to need. IRE1 is the most conserved UPR sensor in eukaryotic cells. It has remained controversial, however, whether mammalian and yeast IRE1 use a common mechanism for ER stress sensing. Here, we...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30700
更新日期:2017-10-03 00:00:00
abstract::Mood and anxiety disorders are the most prevalent psychiatric conditions and are exacerbated by stress. Recent studies have suggested cyclooxygenase-2 (COX-2) inhibition could represent a novel treatment approach or augmentation strategy for affective disorders including anxiety disorders and major depression. We show...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14137
更新日期:2016-05-10 00:00:00
abstract::Metabolic cycles are a fundamental element of cellular and organismal function. Among the most critical in higher organisms is the Cori Cycle, the systemic cycling between lactate and glucose. Here, skeletal muscle-specific Mitochondrial Pyruvate Carrier (MPC) deletion in mice diverted pyruvate into circulating lactat...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45873
更新日期:2019-07-18 00:00:00
abstract::The knirps (kni) locus encodes transcription factors required for induction of the L2 wing vein in Drosophila. Here, we employ diverse CRISPR/Cas9 genome editing tools to generate a series of targeted lesions within the endogenous cis-regulatory module (CRM) required for kni expression in the L2 vein primordium. Pheno...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30281
更新日期:2017-12-23 00:00:00
abstract::Vesicle fusion is mediated by an assembly of SNARE proteins between opposing membranes, but it is unknown whether transmembrane domains (TMDs) of SNARE proteins serve mechanistic functions that go beyond passive anchoring of the force-generating SNAREpin to the fusing membranes. Here, we show that conformational flexi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17571
更新日期:2016-06-25 00:00:00
abstract::The medial subnucleus of the amygdala (MeA) plays a central role in processing sensory cues required for innate behaviors. However, whether there is a link between developmental programs and the emergence of inborn behaviors remains unknown. Our previous studies revealed that the telencephalic preoptic area (POA) embr...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21012
更新日期:2017-03-13 00:00:00
abstract::Transmissible cancers, in which cancer cells themselves act as an infectious agent, have been identified in Tasmanian devils, dogs, and four bivalves. We investigated a disseminated neoplasia affecting geographically distant populations of two species of mussels (Mytilus chilensis in South America and M. edulis in Eur...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47788
更新日期:2019-11-05 00:00:00
abstract::Aberrant expression of cancer genes and non-canonical RNA species is a hallmark of cancer. However, the mechanisms driving such atypical gene expression programs are incompletely understood. Here, our transcriptional profiling of a cohort of 50 primary clear cell renal cell carcinoma (ccRCC) samples from The Cancer Ge...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.09214
更新日期:2015-11-17 00:00:00
abstract::The canonical action of the p85α regulatory subunit of phosphatidylinositol 3-kinase (PI3K) is to associate with the p110α catalytic subunit to allow stimuli-dependent activation of the PI3K pathway. We elucidate a p110α-independent role of homodimerized p85α in the positive regulation of PTEN stability and activity. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.06866
更新日期:2015-07-29 00:00:00