Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio).

abstract：:Follicle rupture, the final step in ovulation, utilizes conserved molecular mechanisms including matrix metalloproteinases (Mmps), steroid signaling, and adrenergic signaling. It is still unknown how follicles become competent for follicle rupture/ovulation. Here, we identify a zinc-finger transcription factor Hindsig...

journal_title：eLife

authors： Deady LD,Li W,Sun J

更新日期：2017-12-19 00:00:00

abstract：:The Cdc45/Mcm2-7/GINS (CMG) helicase separates DNA strands during replication in eukaryotes. How the CMG is assembled and engages DNA substrates remains unclear. Using electron microscopy, we have determined the structure of the CMG in the presence of ATPγS and a DNA duplex bearing a 3' single-stranded tail. The struc...

journal_title：eLife

authors： Costa A,Renault L,Swuec P,Petojevic T,Pesavento JJ,Ilves I,MacLellan-Gibson K,Fleck RA,Botchan MR,Berger JM

更新日期：2014-08-12 00:00:00

abstract：:RNA-protein (RNP) granules have been proposed to assemble by forming solid RNA/protein aggregates or through phase separation into a liquid RNA/protein phase. Which model describes RNP granules in living cells is still unclear. In this study, we analyze P bodies in budding yeast and find that they have liquid-like pro...

journal_title：eLife

authors： Kroschwald S,Maharana S,Mateju D,Malinovska L,Nüske E,Poser I,Richter D,Alberti S

更新日期：2015-08-04 00:00:00

abstract：:Optogenetic approaches are leading to a better understanding of the forces that determine the plane of cell division. ...

journal_title：eLife

authors： Serra-Marques A,Dumont S

更新日期：2018-07-09 00:00:00

abstract：:To move the body, the brain must precisely coordinate patterns of activity among diverse populations of motor neurons. Here, we use in vivo calcium imaging, electrophysiology, and behavior to understand how genetically-identified motor neurons control flexion of the fruit fly tibia. We find that leg motor neurons exhi...

journal_title：eLife

authors： Azevedo AW,Dickinson ES,Gurung P,Venkatasubramanian L,Mann RS,Tuthill JC

更新日期：2020-06-03 00:00:00

abstract：:Most neurogenesis in the mammalian brain is completed embryonically, but in certain areas the production of neurons continues throughout postnatal life. The functional properties of mature postnatally generated neurons often match those of their embryonically produced counterparts. However, we show here that in the ol...

journal_title：eLife

authors： Galliano E,Franzoni E,Breton M,Chand AN,Byrne DJ,Murthy VN,Grubb MS

更新日期：2018-04-20 00:00:00

abstract：:Engineered molecules based on human cytokines have potential uses in research and medicine. ...

journal_title：eLife

authors： Villarino A,O'Shea JJ

更新日期：2017-05-12 00:00:00

abstract：:Myosins play essential roles in the development and function of auditory organs and multiple myosin genes are associated with hereditary forms of deafness. Using a forward genetic screen in Drosophila, we identified an E3 ligase, Ubr3, as an essential gene for auditory organ development. Ubr3 negatively regulates the ...

journal_title：eLife

authors： Li T,Giagtzoglou N,Eberl DF,Jaiswal SN,Cai T,Godt D,Groves AK,Bellen HJ

更新日期：2016-06-22 00:00:00

abstract：:The collapse of iconic, keystone populations of sockeye (Oncorhynchus nerka) and Chinook (Oncorhynchus tshawytscha) salmon in the Northeast Pacific is of great concern. It is thought that infectious disease may contribute to declines, but little is known about viruses endemic to Pacific salmon. Metatranscriptomic sequ...

journal_title：eLife

authors： Mordecai GJ,Miller KM,Di Cicco E,Schulze AD,Kaukinen KH,Ming TJ,Li S,Tabata A,Teffer A,Patterson DA,Ferguson HW,Suttle CA

更新日期：2019-09-03 00:00:00

abstract：:B-cell receptors form ordered clusters to recruit kinases and exclude phosphatases. ...

journal_title：eLife

authors： Bálint Š,Dustin ML

更新日期：2017-03-30 00:00:00

abstract：:Animals seek out relevant information by moving through a dynamic world, but sensory systems are usually studied under highly constrained and passive conditions that may not probe important dimensions of the neural code. Here, we explored neural coding in the barrel cortex of head-fixed mice that tracked walls with th...

journal_title：eLife

authors： Sofroniew NJ,Vlasov YA,Hires SA,Freeman J,Svoboda K

更新日期：2015-12-23 00:00:00

abstract：:Tumors are composed of many different cell types including cancer cells, fibroblasts, and immune cells. Dissecting functional metabolic differences between cell types within a mixed population can be challenging due to the rapid turnover of metabolites relative to the time needed to isolate cells. To overcome this cha...

journal_title：eLife

authors： Lau AN,Li Z,Danai LV,Westermark AM,Darnell AM,Ferreira R,Gocheva V,Sivanand S,Lien EC,Sapp KM,Mayers JR,Biffi G,Chin CR,Davidson SM,Tuveson DA,Jacks T,Matheson NJ,Yilmaz O,Vander Heiden MG

更新日期：2020-07-10 00:00:00

abstract：:Neurotransmitter release requires formation of trans-SNARE complexes between the synaptic vesicle and plasma membranes, which likely underlies synaptic vesicle priming to a release-ready state. It is unknown whether Munc18-1, Munc13-1, complexin-1 and synaptotagmin-1 are important for priming because they mediate tran...

journal_title：eLife

authors： Prinslow EA,Stepien KP,Pan YZ,Xu J,Rizo J

更新日期：2019-01-18 00:00:00

abstract：:TFIID-a complex of TATA-binding protein (TBP) and TBP-associated factors (TAFs)-is a central component of the Pol II promoter recognition apparatus. Recent studies have revealed significant downregulation of TFIID subunits in terminally differentiated myocytes, hepatocytes and adipocytes. Here, we report that TBP prot...

journal_title：eLife

authors： Li L,Martinez SS,Hu W,Liu Z,Tjian R

更新日期：2015-09-22 00:00:00

abstract：:The segregation of the trophectoderm (TE) from the inner cell mass (ICM) in the mouse blastocyst is determined by position-dependent Hippo signaling. However, the window of responsiveness to Hippo signaling, the exact timing of lineage commitment and the overall relationship between cell commitment and global gene exp...

journal_title：eLife

authors： Posfai E,Petropoulos S,de Barros FRO,Schell JP,Jurisica I,Sandberg R,Lanner F,Rossant J

更新日期：2017-02-22 00:00:00

abstract：:Homologous recombination is widespread and catalyzes evolution. Nonetheless, its existence in animal mitochondrial DNA is questioned. We designed selections for recombination between co-resident mitochondrial genomes in various heteroplasmic Drosophila lines. In four experimental settings, recombinant genomes became t...

journal_title：eLife

authors： Ma H,O'Farrell PH

更新日期：2015-08-03 00:00:00

abstract：:An 'interactome' screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interacto...

journal_title：eLife

authors： Li H,Watson A,Olechwier A,Anaya M,Sorooshyari SK,Harnett DP,Lee HP,Vielmetter J,Fares MA,Garcia KC,Özkan E,Labrador JP,Zinn K

更新日期：2017-08-15 00:00:00

abstract：:Transmissible cancers, in which cancer cells themselves act as an infectious agent, have been identified in Tasmanian devils, dogs, and four bivalves. We investigated a disseminated neoplasia affecting geographically distant populations of two species of mussels (Mytilus chilensis in South America and M. edulis in Eur...

journal_title：eLife

authors： Yonemitsu MA,Giersch RM,Polo-Prieto M,Hammel M,Simon A,Cremonte F,Avilés FT,Merino-Véliz N,Burioli EA,Muttray AF,Sherry J,Reinisch C,Baldwin SA,Goff SP,Houssin M,Arriagada G,Vázquez N,Bierne N,Metzger MJ

更新日期：2019-11-05 00:00:00

abstract：:Responses of sensory neurons represent stimulus information, but are also influenced by internal state. For example, when monkeys direct their attention to a visual stimulus, the response gain of specific subsets of neurons in visual cortex changes. Here, we develop a functional model of population activity to investi...

journal_title：eLife

authors： Rabinowitz NC,Goris RL,Cohen M,Simoncelli EP

更新日期：2015-11-02 00:00:00

abstract：:Deinococcus radiodurans (DR) survives in the presence of hundreds of double-stranded DNA (dsDNA) breaks by efficiently repairing such breaks. RecO, a protein that is essential for the extreme radioresistance of DR, is one of the major recombination mediator proteins in the RecA-loading process in the RecFOR pathway. H...

journal_title：eLife

authors： Hwang J,Kim JY,Kim C,Park S,Joo S,Kim SK,Lee NK

更新日期：2020-04-16 00:00:00

abstract：:Hepatitis B virus (HBV) is an important but difficult to study human pathogen. Most basics of the hepadnaviral life-cycle were unraveled using duck HBV (DHBV) as a model although DHBV has a capsid protein (CP) comprising ~260 rather than ~180 amino acids. Here we present high-resolution structures of several DHBV caps...

journal_title：eLife

authors： Makbul C,Nassal M,Böttcher B

更新日期：2020-08-14 00:00:00

abstract：:Whether neurons encode information through their spike rates, their activity times or both is an ongoing debate in systems neuroscience. Here, we tested whether humans can discriminate between a pair of temporal odor mixtures (TOMs) composed of the same two components delivered in rapid succession in either one tempor...

journal_title：eLife

authors： Perl O,Nahum N,Belelovsky K,Haddad R

更新日期：2020-02-07 00:00:00

abstract：:In Drosophila larvae, Class IV sensory neurons respond to noxious thermal stimuli and provoke heat avoidance behavior. Previously, we showed that the activated neurons displayed characteristic fluctuations of firing rates, which consisted of repetitive high-frequency spike trains and subsequent pause periods, and we p...

journal_title：eLife

authors： Onodera K,Baba S,Murakami A,Uemura T,Usui T

更新日期：2017-10-16 00:00:00

abstract：:In the retina, synaptic transmission between photoreceptors and downstream ON-bipolar neurons (ON-BCs) is mediated by a GPCR pathway, which plays an essential role in vision. However, the mechanisms that control signal transmission at this synapse and its relevance to behavior remain poorly understood. In this study w...

journal_title：eLife

authors： Sarria I,Pahlberg J,Cao Y,Kolesnikov AV,Kefalov VJ,Sampath AP,Martemyanov KA

更新日期：2015-04-16 00:00:00

abstract：:Proteasome inhibition elicits an evolutionarily conserved response wherein proteasome subunit mRNAs are upregulated, resulting in recovery (i.e., 'bounce-back') of proteasome activity. We previously demonstrated that the transcription factor Nrf1/NFE2L1 mediates this homeostatic response in mammalian cells. We show he...

journal_title：eLife

authors： Radhakrishnan SK,den Besten W,Deshaies RJ

更新日期：2014-01-01 00:00:00

abstract：:Protein concentration gradients pattern developing organisms and single cells. In Schizosaccharomyces pombe rod-shaped cells, Pom1 kinase forms gradients with maxima at cell poles. Pom1 controls the timing of mitotic entry by inhibiting Cdr2, which forms stable membrane-associated nodes at mid-cell. Pom1 gradients rel...

journal_title：eLife

authors： Gerganova V,Floderer C,Archetti A,Michon L,Carlini L,Reichler T,Manley S,Martin SG

更新日期：2019-05-03 00:00:00

abstract：:In many daily tasks, we make multiple decisions before reaching a goal. In order to learn such sequences of decisions, a mechanism to link earlier actions to later reward is necessary. Reinforcement learning (RL) theory suggests two classes of algorithms solving this credit assignment problem: In classic temporal-diff...

journal_title：eLife

authors： Lehmann MP,Xu HA,Liakoni V,Herzog MH,Gerstner W,Preuschoff K

更新日期：2019-11-11 00:00:00

abstract：:Previous research showed that spontaneous neuronal activity presents sloppiness: the collective behavior is strongly determined by a small number of parameter combinations, defined as 'stiff' dimensions, while it is insensitive to many others ('sloppy' dimensions). Here, we analyzed neural population activity from the...

journal_title：eLife

authors： Ponce-Alvarez A,Mochol G,Hermoso-Mendizabal A,de la Rocha J,Deco G

更新日期：2020-03-17 00:00:00

abstract：:Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Here, we describe a set of resources to streamline reporter gene knock-ins in zebrafish and demonstrate the broader utility of the method in mammalian cells. Our approach uses short homology of 24-...

journal_title：eLife

authors： Wierson WA,Welker JM,Almeida MP,Mann CM,Webster DA,Torrie ME,Weiss TJ,Kambakam S,Vollbrecht MK,Lan M,McKeighan KC,Levey J,Ming Z,Wehmeier A,Mikelson CS,Haltom JA,Kwan KM,Chien CB,Balciunas D,Ekker SC,Clark KJ,We

更新日期：2020-05-15 00:00:00

abstract：:Cognitive control proactively configures information processing to suit expected task demands. Predictions of forthcoming demand can be driven by explicit external cues or be generated internally, based on past experience (cognitive history). However, it is not known whether and how the brain reconciles these two sour...

journal_title：eLife

authors： Jiang J,Wagner AD,Egner T

更新日期：2018-08-16 00:00:00