Abstract:
:An 'interactome' screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification of many interactions in a single sample. Using the BPIA, we 'deorphanized' four more members of the Beat-Side network. We confirmed interactions using surface plasmon resonance. The expression patterns of beat and side genes suggest that Beats are neuronal receptors for Sides expressed on peripheral tissues. side-VI is expressed in muscle fibers targeted by the ISNb nerve, as well as at growth cone choice points and synaptic targets for the ISN and TN nerves. beat-V genes, encoding Side-VI receptors, are expressed in ISNb and ISN motor neurons.
journal_name
Elifejournal_title
eLifeauthors
Li H,Watson A,Olechwier A,Anaya M,Sorooshyari SK,Harnett DP,Lee HP,Vielmetter J,Fares MA,Garcia KC,Özkan E,Labrador JP,Zinn Kdoi
10.7554/eLife.28111subject
Has Abstractpub_date
2017-08-15 00:00:00issn
2050-084Xpii
28111journal_volume
6pub_type
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