A robust activity marking system for exploring active neuronal ensembles.

abstract：:Previously, we showed that serum and monocytes from patients with CF exhibit an enhanced NLRP3-inflammasome signature with increased IL-18, IL-1β, caspase-1 activity and ASC speck release (Scambler et al. eLife 2019). Here we show that CFTR modulators down regulate this exaggerated proinflammatory response following L...

journal_title：eLife

authors： Jarosz-Griffiths HH,Scambler T,Wong CH,Lara-Reyna S,Holbrook J,Martinon F,Savic S,Whitaker P,Etherington C,Spoletini G,Clifton I,Mehta A,McDermott MF,Peckham D

更新日期：2020-03-02 00:00:00

abstract：:Purkinje neurons are central to cerebellar function and show membrane bistability when recorded in vitro or in vivo under anesthesia. The existence of bistability in vivo in awake animals is disputed. Here, by recording intracellularly from Purkinje neurons in unanesthetized larval zebrafish (Danio rerio), we unequivo...

journal_title：eLife

authors： Sengupta M,Thirumalai V

更新日期：2015-09-29 00:00:00

abstract：:Nuclear speckles (NS) are among the most prominent biomolecular condensates. Despite their prevalence, research on the function of NS is virtually restricted to colocalization analyses, since an organizing core, without which NS cannot form, remains unidentified. The monoclonal antibody SC35, raised against a spliceos...

journal_title：eLife

authors： Ilik İA,Malszycki M,Lübke AK,Schade C,Meierhofer D,Aktaş T

更新日期：2020-10-23 00:00:00

abstract：:Visual perception and imagery rely on similar representations in the visual cortex. During perception, visual activity is characterized by distinct processing stages, but the temporal dynamics underlying imagery remain unclear. Here, we investigated the dynamics of visual imagery in human participants using magnetoenc...

journal_title：eLife

authors： Dijkstra N,Mostert P,Lange FP,Bosch S,van Gerven MA

更新日期：2018-05-29 00:00:00

abstract：:T follicular helper cells (Tfh) are critical for the longevity and quality of antibody-mediated protection against infection. Yet few signaling pathways have been identified to be unique solely to Tfh development. ROQUIN is a post-transcriptional repressor of T cells, acting through its ROQ domain to destabilize mRNA ...

journal_title：eLife

authors： Ramiscal RR,Parish IA,Lee-Young RS,Babon JJ,Blagih J,Pratama A,Martin J,Hawley N,Cappello JY,Nieto PF,Ellyard JI,Kershaw NJ,Sweet RA,Goodnow CC,Jones RG,Febbraio MA,Vinuesa CG,Athanasopoulos V

更新日期：2015-10-23 00:00:00

abstract：:Over the past century, soybean oil (SBO) consumption in the United States increased dramatically. The main SBO fatty acid, linoleic acid (18:2), inhibits in vitro the growth of lactobacilli, beneficial members of the small intestinal microbiota. Human-associated lactobacilli have declined in prevalence in Western micr...

journal_title：eLife

authors： Di Rienzi SC,Jacobson J,Kennedy EA,Bell ME,Shi Q,Waters JL,Lawrence P,Brenna JT,Britton RA,Walter J,Ley RE

更新日期：2018-03-27 00:00:00

abstract：:Bacterial flagella mediate host-microbe interactions through tissue tropism during colonization, as well as by activating immune responses. The flagellar shaft of some bacteria, including several human pathogens, is encased in a membranous sheath of unknown function. While it has been hypothesized that the sheath may ...

journal_title：eLife

authors： Brennan CA,Hunt JR,Kremer N,Krasity BC,Apicella MA,McFall-Ngai MJ,Ruby EG

更新日期：2014-03-04 00:00:00

abstract：:Two different cell signals often affect transcription of the same gene. In such cases, it is natural to ask how the combined transcriptional response compares to the individual responses. The most commonly used mechanistic models predict additive or multiplicative combined responses, but a systematic genome-wide evalu...

journal_title：eLife

authors： Sanford EM,Emert BL,Coté A,Raj A

更新日期：2020-12-07 00:00:00

abstract：:Human-based modelling and simulations are becoming ubiquitous in biomedical science due to their ability to augment experimental and clinical investigations. Cardiac electrophysiology is one of the most advanced areas, with cardiac modelling and simulation being considered for virtual testing of pharmacological therap...

journal_title：eLife

authors： Tomek J,Bueno-Orovio A,Passini E,Zhou X,Minchole A,Britton O,Bartolucci C,Severi S,Shrier A,Virag L,Varro A,Rodriguez B

更新日期：2019-12-24 00:00:00

abstract：:The canonical action of the p85α regulatory subunit of phosphatidylinositol 3-kinase (PI3K) is to associate with the p110α catalytic subunit to allow stimuli-dependent activation of the PI3K pathway. We elucidate a p110α-independent role of homodimerized p85α in the positive regulation of PTEN stability and activity. ...

journal_title：eLife

authors： Cheung LW,Walkiewicz KW,Besong TM,Guo H,Hawke DH,Arold ST,Mills GB

更新日期：2015-07-29 00:00:00

abstract：:Signaling molecules activate distinct patterns of gene expression to coordinate embryogenesis, but how spatiotemporal expression diversity is generated is an open question. In zebrafish, a BMP signaling gradient patterns the dorsal-ventral axis. We systematically identified target genes responding to BMP and found tha...

journal_title：eLife

authors： Rogers KW,ElGamacy M,Jordan BM,Müller P

更新日期：2020-11-11 00:00:00

abstract：:Neural computations underlying cognitive functions require calibration of the strength of excitatory and inhibitory synaptic connections and are associated with modulation of gamma frequency oscillations in network activity. However, principles relating gamma oscillations, synaptic strength and circuit computations ar...

journal_title：eLife

authors： Solanka L,van Rossum MC,Nolan MF

更新日期：2015-07-06 00:00:00

abstract：:WW domain tandem-containing proteins such as KIBRA, YAP, and MAGI play critical roles in cell growth and polarity via binding to and positioning target proteins in specific subcellular regions. An immense disparity exists between promiscuity of WW domain-mediated target bindings and specific roles of WW domain protein...

journal_title：eLife

authors： Lin Z,Yang Z,Xie R,Ji Z,Guan K,Zhang M

更新日期：2019-09-05 00:00:00

abstract：:Burkholderia thailandensis uses acyl-homoserine lactone-mediated quorum sensing systems to regulate hundreds of genes. Here we show that cell-cell contact-dependent type VI secretion (T6S) toxin-immunity systems are among those activated by quorum sensing in B. thailandensis. We also demonstrate that T6S is required t...

journal_title：eLife

authors： Majerczyk C,Schneider E,Greenberg EP

更新日期：2016-05-16 00:00:00

abstract：:Genomic sequencing has implicated large numbers of genes and de novo mutations as potential disease risk factors. A high throughput in vivo model system is needed to validate gene associations with pathology. We developed a Drosophila-based functional system to screen candidate disease genes identified from Congenital...

journal_title：eLife

authors： Zhu JY,Fu Y,Nettleton M,Richman A,Han Z

更新日期：2017-01-20 00:00:00

abstract：:Down syndrome (DS) results in various degrees of cognitive deficits. In DS mouse models, recovery of behavioral and neurophysiological deficits using GABAAR antagonists led to hypothesize an excessive activity of inhibitory circuits in this condition. Nonetheless, whether over-inhibition is present in DS and whether t...

journal_title：eLife

authors： Zorrilla de San Martin J,Donato C,Peixoto J,Aguirre A,Choudhary V,De Stasi AM,Lourenço J,Potier MC,Bacci A

更新日期：2020-08-12 00:00:00

abstract：:Aberrant expression of cancer genes and non-canonical RNA species is a hallmark of cancer. However, the mechanisms driving such atypical gene expression programs are incompletely understood. Here, our transcriptional profiling of a cohort of 50 primary clear cell renal cell carcinoma (ccRCC) samples from The Cancer Ge...

journal_title：eLife

authors： Grosso AR,Leite AP,Carvalho S,Matos MR,Martins FB,Vítor AC,Desterro JM,Carmo-Fonseca M,de Almeida SF

更新日期：2015-11-17 00:00:00

abstract：:Recent lineage tracing analyses have significantly improved our understanding of immune system development and highlighted the importance of the different hematopoietic waves. The current challenge is to understand whether these waves interact and whether this affects the function of the immune system. Here we report ...

journal_title：eLife

authors： Bazzi W,Cattenoz PB,Delaporte C,Dasari V,Sakr R,Yuasa Y,Giangrande A

更新日期：2018-07-11 00:00:00

abstract：:Synaptic positions underlie precise circuit connectivity. Synaptic positions can be established during embryogenesis and sustained during growth. The mechanisms that sustain synaptic specificity during allometric growth are largely unknown. We performed forward genetic screens in C. elegans for regulators of this proc...

journal_title：eLife

authors： Fan J,Ji T,Wang K,Huang J,Wang M,Manning L,Dong X,Shi Y,Zhang X,Shao Z,Colón-Ramos DA

更新日期：2020-04-07 00:00:00

abstract：:Meiotic crossovers result from homology-directed repair of DNA double-strand breaks (DSBs). Unlike yeast and plants, where DSBs are generated near gene promoters, in many vertebrates DSBs are enriched at hotspots determined by the DNA binding activity of the rapidly evolving zinc finger array of PRDM9 (PR domain zinc ...

journal_title：eLife

authors： Mahgoub M,Paiano J,Bruno M,Wu W,Pathuri S,Zhang X,Ralls S,Cheng X,Nussenzweig A,Macfarlan TS

更新日期：2020-04-30 00:00:00

abstract：:Studies in different animal model systems have revealed the impact of odors on immune cells; however, any understanding on why and how odors control cellular immunity remained unclear. We find that Drosophila employ an olfactory-immune cross-talk to tune a specific cell type, the lamellocytes, from hematopoietic-proge...

journal_title：eLife

authors： Madhwal S,Shin M,Kapoor A,Goyal M,Joshi MK,Ur Rehman PM,Gor K,Shim J,Mukherjee T

更新日期：2020-12-29 00:00:00

abstract：:Indirect evidence suggests that blastopore closure during gastrulation of anamniotes, including amphibians such as Xenopus laevis, depends on circumblastoporal convergence forces generated by the marginal zone (MZ), but direct evidence is lacking. We show that explanted MZs generate tensile convergence forces up to 1....

journal_title：eLife

authors： Shook DR,Kasprowicz EM,Davidson LA,Keller R

更新日期：2018-03-13 00:00:00

abstract：:A heat exchange mechanism in the head of kissing bugs helps to prevent stress and regulate their temperature while they feed on warm blood. ...

journal_title：eLife

authors： Benoit JB,Denlinger DL

更新日期：2017-11-21 00:00:00

abstract：:During outbreaks of high-consequence pathogens, airport screening programs have been deployed to curtail geographic spread of infection. The effectiveness of screening depends on several factors, including pathogen natural history and epidemiology, human behavior, and characteristics of the source epidemic. We develop...

journal_title：eLife

authors： Gostic KM,Kucharski AJ,Lloyd-Smith JO

更新日期：2015-02-19 00:00:00

abstract：Background:Malaria elimination strategies require a thorough understanding of parasite transmission from human to mosquito. A clinical model to induce gametocytes to understand their dynamics and evaluate transmission-blocking interventions (TBI) is currently unavailable. Here, we explore the use of the well-establishe...

journal_title：eLife

authors： Reuling IJ,van de Schans LA,Coffeng LE,Lanke K,Meerstein-Kessel L,Graumans W,van Gemert GJ,Teelen K,Siebelink-Stoter R,van de Vegte-Bolmer M,de Mast Q,van der Ven AJ,Ivinson K,Hermsen CC,de Vlas S,Bradley J,Collins KA,O

更新日期：2018-02-27 00:00:00

abstract：:Making inferences about the computations performed by neuronal circuits from synapse-level connectivity maps is an emerging opportunity in neuroscience. The mushroom body (MB) is well positioned for developing and testing such an approach due to its conserved neuronal architecture, recently completed dense connectome,...

journal_title：eLife

authors： Li F,Lindsey JW,Marin EC,Otto N,Dreher M,Dempsey G,Stark I,Bates AS,Pleijzier MW,Schlegel P,Nern A,Takemura SY,Eckstein N,Yang T,Francis A,Braun A,Parekh R,Costa M,Scheffer LK,Aso Y,Jefferis GS,Abbott LF,Litwi

更新日期：2020-12-14 00:00:00

abstract：:Genetic programming and neural activity drive synaptic remodeling in developing neural circuits, but the molecular components that link these pathways are poorly understood. Here we show that the C. elegans Degenerin/Epithelial Sodium Channel (DEG/ENaC) protein, UNC-8, is transcriptionally controlled to function as a ...

journal_title：eLife

authors： Miller-Fleming TW,Petersen SC,Manning L,Matthewman C,Gornet M,Beers A,Hori S,Mitani S,Bianchi L,Richmond J,Miller DM

更新日期：2016-07-12 00:00:00

abstract：:The transcription factor ASCIZ (ATMIN, ZNF822) has an unusually high number of recognition motifs for the product of its main target gene, the hub protein LC8 (DYNLL1). Using a combination of biophysical methods, structural analysis by NMR and electron microscopy, and cellular transcription assays, we developed a mode...

journal_title：eLife

authors： Clark S,Myers JB,King A,Fiala R,Novacek J,Pearce G,Heierhorst J,Reichow SL,Barbar EJ

更新日期：2018-05-01 00:00:00

abstract：:Determining the etiologic basis of the mutations that are responsible for cancer is one of the fundamental challenges in modern cancer research. Different mutational processes induce different types of DNA mutations, providing 'mutational signatures' that have led to key insights into cancer etiology. The most widely ...

journal_title：eLife

authors： Afsari B,Kuo A,Zhang Y,Li L,Lahouel K,Danilova L,Favorov A,Rosenquist TA,Grollman AP,Kinzler KW,Cope L,Vogelstein B,Tomasetti C

更新日期：2021-01-25 00:00:00

abstract：:IQ scores are correlated with the morphology and activity of certain neurons in the human temporal cortex. ...

journal_title：eLife

authors： Miller EN,Sherwood CC

更新日期：2019-02-05 00:00:00