An extended retinotopic map of mouse cortex.

abstract：:CpG islands are gene regulatory elements associated with the majority of mammalian promoters, yet how they regulate gene expression remains poorly understood. Here, we identify FBXL19 as a CpG island-binding protein in mouse embryonic stem (ES) cells and show that it associates with the CDK-Mediator complex. We discov...

journal_title：eLife

authors： Dimitrova E,Kondo T,Feldmann A,Nakayama M,Koseki Y,Konietzny R,Kessler BM,Koseki H,Klose RJ

更新日期：2018-05-29 00:00:00

abstract：:A protein called P36 holds the key to how different species of malaria parasite invade liver cells. ...

journal_title：eLife

authors： Billker O

更新日期：2017-06-14 00:00:00

abstract：:Macrophages are highly plastic cells with critical roles in immunity, cancer, and tissue homeostasis, but how these distinct cellular fates are triggered by environmental cues is poorly understood. To uncover how primary murine macrophages respond to bacterial pathogens, we globally assessed changes in post-translatio...

journal_title：eLife

authors： Budzik JM,Swaney DL,Jimenez-Morales D,Johnson JR,Garelis NE,Repasy T,Roberts AW,Popov LM,Parry TJ,Pratt D,Ideker T,Krogan NJ,Cox JS

更新日期：2020-01-17 00:00:00

abstract：:Development of specialized cells and structures in the heart is regulated by spatially -restricted molecular pathways. Disruptions in these pathways can cause severe congenital cardiac malformations or functional defects. To better understand these pathways and how they regulate cardiac development we used tomo-seq, c...

journal_title：eLife

authors： Burkhard SB,Bakkers J

更新日期：2018-02-05 00:00:00

abstract：:We generated induced excitatory neurons (iNeurons, iNs) from chimpanzee, bonobo, and human stem cells by expressing the transcription factor neurogenin-2 (NGN2). Single-cell RNA sequencing showed that genes involved in dendrite and synapse development are expressed earlier during iNs maturation in the chimpanzee and b...

journal_title：eLife

authors： Schörnig M,Ju X,Fast L,Ebert S,Weigert A,Kanton S,Schaffer T,Nadif Kasri N,Treutlein B,Peter BM,Hevers W,Taverna E

更新日期：2021-01-20 00:00:00

abstract：:The controversy surrounding 'gain-of-function' experiments on high-consequence avian influenza viruses has highlighted the role of ferret transmission experiments in studying the transmission potential of novel influenza strains. However, the mapping between influenza transmission in ferrets and in humans is unsubstan...

journal_title：eLife

authors： Buhnerkempe MG,Gostic K,Park M,Ahsan P,Belser JA,Lloyd-Smith JO

更新日期：2015-09-02 00:00:00

abstract：:Modern neuroscience research often requires the coordination of multiple processes such as stimulus generation, real-time experimental control, as well as behavioral and neural measurements. The technical demands required to simultaneously manage these processes with high temporal fidelity is a barrier that limits the...

journal_title：eLife

authors： Kim B,Kenchappa SC,Sunkara A,Chang TY,Thompson L,Doudlah R,Rosenberg A

更新日期：2019-02-07 00:00:00

abstract：:In addition to their role in desensitization and internalization of G protein-coupled receptors (GPCRs), β-arrestins are essential scaffolds linking GPCRs to Erk1/2 signaling. However, their role in GPCR-operated Erk1/2 activation differs between GPCRs and the underlying mechanism remains poorly characterized. Here, w...

journal_title：eLife

authors： Cassier E,Gallay N,Bourquard T,Claeysen S,Bockaert J,Crépieux P,Poupon A,Reiter E,Marin P,Vandermoere F

更新日期：2017-02-07 00:00:00

abstract：:Intergroup conflict contributes to human discrimination and violence, but persists because individuals make costly contributions to their group's fighting capacity. Yet, how group members effectively coordinate their contributions during intergroup conflict remains poorly understood. Here, we examine the role of oxyto...

journal_title：eLife

authors： Zhang H,Gross J,De Dreu C,Ma Y

更新日期：2019-01-25 00:00:00

abstract：:The roles of long non-coding RNAs (lncRNAs) in regulating cancer and stem cells are being increasingly appreciated. Its diverse mechanisms provide the regulatory network with a bigger repertoire to increase complexity. Here we report a novel LncRNA, Lnc34a, that is enriched in colon cancer stem cells (CCSCs) and initi...

journal_title：eLife

authors： Wang L,Bu P,Ai Y,Srinivasan T,Chen HJ,Xiang K,Lipkin SM,Shen X

更新日期：2016-04-14 00:00:00

abstract：:In endoplasmic reticulum-associated protein degradation (ERAD), membrane proteins are ubiquitinated, extracted from the membrane, and degraded by the proteasome. The cytosolic ATPase Cdc48 drives extraction by pulling on polyubiquitinated substrates. How hydrophobic transmembrane (TM) segments are moved from the phosp...

journal_title：eLife

authors： Schmidt CC,Vasic V,Stein A

更新日期：2020-06-26 00:00:00

abstract：:Autophagy has been linked to longevity in many species, but the underlying mechanisms are unclear. Using a GFP-tagged and a new tandem-tagged Atg8/LGG-1 reporter, we quantified autophagic vesicles and performed autophagic flux assays in multiple tissues of wild-type Caenorhabditis elegans and long-lived daf-2/insulin/...

journal_title：eLife

authors： Chang JT,Kumsta C,Hellman AB,Adams LM,Hansen M

更新日期：2017-07-04 00:00:00

abstract：:Learning more about the cells that enable parasitic worms called schistosomes to reproduce inside snails could lead to new treatments that prevent these parasites from being transmitted to humans. ...

journal_title：eLife

authors： Sánchez Alvarado A

更新日期：2013-07-30 00:00:00

abstract：:When a female fly mates it produces a hormone that increases the size of its midgut and enhances fat metabolism in order to provide the energy needed for reproduction. ...

journal_title：eLife

authors： Flatt T

更新日期：2015-07-28 00:00:00

abstract：:Many postdoctoral researchers apply for faculty positions knowing relatively little about the hiring process or what is needed to secure a job offer. To address this lack of knowledge about the hiring process we conducted a survey of applicants for faculty positions: the survey ran between May 2018 and May 2019, and r...

journal_title：eLife

authors： Fernandes JD,Sarabipour S,Smith CT,Niemi NM,Jadavji NM,Kozik AJ,Holehouse AS,Pejaver V,Symmons O,Bisson Filho AW,Haage A

更新日期：2020-06-12 00:00:00

abstract：:Microbes are embedded in complex communities where they engage in a wide array of intra- and inter-specific interactions. The extent to which these interactions drive or impede microbiome diversity is not well understood. Historically, two contrasting hypotheses have been suggested to explain how species interactions ...

journal_title：eLife

authors： Madi N,Vos M,Murall CL,Legendre P,Shapiro BJ

更新日期：2020-11-20 00:00:00

abstract：:The design of synthetic optogenetic tools that allow precise spatiotemporal control of biological processes previously inaccessible to optogenetic control has developed rapidly over the last years. Rational design of such tools requires detailed knowledge of allosteric light signaling in natural photoreceptors. To und...

journal_title：eLife

authors： Heintz U,Schlichting I

更新日期：2016-01-12 00:00:00

abstract：:Rapid diagnostic tests (RDTs) have transformed malaria diagnosis. The most prevalent P. falciparum RDTs detect histidine-rich protein 2 (PfHRP2). However, pfhrp2 gene deletions yielding false-negative RDTs, first reported in South America in 2010, have been confirmed in Africa and Asia. We developed a mathematical mod...

journal_title：eLife

authors： Watson OJ,Slater HC,Verity R,Parr JB,Mwandagalirwa MK,Tshefu A,Meshnick SR,Ghani AC

更新日期：2017-08-24 00:00:00

abstract：:The calcium release-activated calcium channel Orai regulates Ca2+ entry into non-excitable cells and is required for proper immune function. While the channel typically opens following Ca2+ release from the endoplasmic reticulum, certain pathologic mutations render the channel constitutively open. Previously, using on...

journal_title：eLife

authors： Hou X,Outhwaite IR,Pedi L,Long SB

更新日期：2020-11-30 00:00:00

abstract：:Being exposed to cannabinoids in the womb has different consequences for male and female rats. ...

journal_title：eLife

authors： Musardo S,Bellone C

更新日期：2018-09-28 00:00:00

abstract：:Probabilistic models of adaptive immune repertoire sequence distributions can be used to infer the expansion of immune cells in response to stimulus, differentiate genetic from environmental factors that determine repertoire sharing, and evaluate the suitability of various target immune sequences for stimulation via v...

journal_title：eLife

authors： Davidsen K,Olson BJ,DeWitt WS 3rd,Feng J,Harkins E,Bradley P,Matsen FA 4th

更新日期：2019-09-05 00:00:00

abstract：:Cerebral cortical architecture at birth encodes regionally differential dendritic arborization and synaptic formation. It underlies behavioral emergence of 2-year-olds. Brain changes in 0-2 years are most dynamic across the lifespan. Effective prediction of future behavior with brain microstructure at birth will revea...

journal_title：eLife

authors： Ouyang M,Peng Q,Jeon T,Heyne R,Chalak L,Huang H

更新日期：2020-12-22 00:00:00

abstract：:Sleep affects brain activity globally, but many cortical sleep waves are spatially confined. Local rhythms serve cortical area-specific sleep needs and functions; however, mechanisms controlling locality are unclear. We identify the thalamic reticular nucleus (TRN) as a source for local, sensory-cortex-specific non-ra...

journal_title：eLife

authors： Fernandez LM,Vantomme G,Osorio-Forero A,Cardis R,Béard E,Lüthi A

更新日期：2018-12-25 00:00:00

abstract：:A spring-loaded mechanism can explain the activation process for a protein that has a crucial role in maintaining the genomic integrity of immature eggs cells. ...

journal_title：eLife

authors： Amelio I,Melino G

更新日期：2016-05-23 00:00:00

abstract：:Daily rhythms of food anticipatory activity (FAA) are regulated independently of the suprachiasmatic nucleus, which mediates entrainment of rhythms to light, but the neural circuits that establish FAA remain elusive. In this study, we show that mice lacking the dopamine D1 receptor (D1R KO mice) manifest greatly reduc...

journal_title：eLife

authors： Gallardo CM,Darvas M,Oviatt M,Chang CH,Michalik M,Huddy TF,Meyer EE,Shuster SA,Aguayo A,Hill EM,Kiani K,Ikpeazu J,Martinez JS,Purpura M,Smit AN,Patton DF,Mistlberger RE,Palmiter RD,Steele AD

更新日期：2014-09-12 00:00:00

abstract：:Understanding the molecular mechanism of glaucoma and development of neuroprotectants is significantly hindered by the lack of a reliable animal model that accurately recapitulates human glaucoma. Here, we sought to develop a mouse model for the secondary glaucoma that is often observed in humans after silicone oil (S...

journal_title：eLife

authors： Zhang J,Li L,Huang H,Fang F,Webber HC,Zhuang P,Liu L,Dalal R,Tang PH,Mahajan VB,Sun Y,Li S,Zhang M,Goldberg JL,Hu Y

更新日期：2019-05-15 00:00:00

abstract：:We determined whether the mutations found in ovarian cancers could be identified in the patients' ovarian cyst fluids. Tumor-specific mutations were detectable in the cyst fluids of 19 of 23 (83%) borderline tumors, 10 of 13 (77%) type I cancers, and 18 of 18 (100%) type II cancers. In contrast, no mutations were foun...

journal_title：eLife

authors： Wang Y,Sundfeldt K,Mateoiu C,Shih IeM,Kurman RJ,Schaefer J,Silliman N,Kinde I,Springer S,Foote M,Kristjansdottir B,James N,Kinzler KW,Papadopoulos N,Diaz LA,Vogelstein B

更新日期：2016-07-15 00:00:00

abstract：:The mechanisms underlying axon regeneration in mature neurons are relevant to the understanding of normal nervous system maintenance and for developing therapeutic strategies for injury. Here, we report novel pathways in axon regeneration, identified by extending our previous function-based screen using the C. elegans...

journal_title：eLife

authors： Kim KW,Tang NH,Piggott CA,Andrusiak MG,Park S,Zhu M,Kurup N,Cherra SJ 3rd,Wu Z,Chisholm AD,Jin Y

更新日期：2018-11-21 00:00:00

abstract：:Compartmentalized oscillations of second messengers affect global cellular signaling. ...

journal_title：eLife

authors： Kuhn SM,Nadler A

更新日期：2020-11-17 00:00:00

abstract：:Bacteria of the genus Shigella cause shigellosis, a severe gastrointestinal disease that is a major cause of diarrhea-associated mortality in humans. Mice are highly resistant to Shigella and the lack of a tractable physiological model of shigellosis has impeded our understanding of this important human disease. Here,...

journal_title：eLife

authors： Mitchell PS,Roncaioli JL,Turcotte EA,Goers L,Chavez RA,Lee AY,Lesser CF,Rauch I,Vance RE

更新日期：2020-10-19 00:00:00