Abstract:
:Migration of skeletal muscle precursor cells is a key step during limb muscle development and depends on the activity of PAX3 and MET. Here, we demonstrate that BRAF serves a crucial function in formation of limb skeletal muscles during mouse embryogenesis downstream of MET and acts as a potent inducer of myoblast cell migration. We found that a fraction of BRAF accumulates in the nucleus after activation and endosomal transport to a perinuclear position. Mass spectrometry based screening for potential interaction partners revealed that BRAF interacts and phosphorylates PAX3. Mutation of BRAF dependent phosphorylation sites in PAX3 impaired the ability of PAX3 to promote migration of C2C12 myoblasts indicating that BRAF directly activates PAX3. Since PAX3 stimulates transcription of the Met gene we propose that MET signaling via BRAF fuels a positive feedback loop, which maintains high levels of PAX3 and MET activity required for limb muscle precursor cell migration.
journal_name
Elifejournal_title
eLifeauthors
Shin J,Watanabe S,Hoelper S,Krüger M,Kostin S,Pöling J,Kubin T,Braun Tdoi
10.7554/eLife.18351subject
Has Abstractpub_date
2016-12-01 00:00:00issn
2050-084Xjournal_volume
5pub_type
杂志文章相关文献
eLife文献大全abstract::We studied the role of the synaptic ribbon for sound encoding at the synapses between inner hair cells (IHCs) and spiral ganglion neurons (SGNs) in mice lacking RIBEYE (RBEKO/KO). Electron and immunofluorescence microscopy revealed a lack of synaptic ribbons and an assembly of several small active zones (AZs) at each ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.29275
更新日期:2018-01-12 00:00:00
abstract::Mechanical force and Wnt signaling activate β-catenin-mediated transcription to promote proliferation and tissue expansion. However, it is unknown whether mechanical force and Wnt signaling act independently or synergize to activate β-catenin signaling and cell division. We show that mechanical strain induced Src-depe...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19799
更新日期:2016-10-26 00:00:00
abstract::The ellipsoid body (EB) in the Drosophila brain is a central complex (CX) substructure that harbors circumferentially laminated ring (R) neuron axons and mediates multifaceted sensory integration and motor coordination functions. However, what regulates R axon lamination and how lamination affects R neuron function re...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25328
更新日期:2017-06-20 00:00:00
abstract::A protein called P36 holds the key to how different species of malaria parasite invade liver cells. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.28600
更新日期:2017-06-14 00:00:00
abstract::V-ATPase is an energy converting enzyme, coupling ATP hydrolysis/synthesis in the hydrophilic V1 domain, with proton flow through the Vo membrane domain, via rotation of the central rotor complex relative to the surrounding stator apparatus. Upon dissociation from the V1 domain, the Vo domain of the eukaryotic V-ATPas...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56862
更新日期:2020-07-08 00:00:00
abstract::Periodontal disease is an age-associated disorder clinically defined by periodontal bone loss, inflammation of the specialized tissues that surround and support the tooth, and microbiome dysbiosis. Currently, there is no therapy for reversing periodontal disease, and treatment is generally restricted to preventive mea...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54318
更新日期:2020-04-28 00:00:00
abstract::Humans and other animals routinely identify and attend to sensory stimuli so as to rapidly acquire rewards or avoid aversive experiences. Emotional arousal, a process mediated by the amygdala, can enhance attention to stimuli in a non-spatial manner. However, amygdala neural activity was recently shown to encode spati...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.04478
更新日期:2014-10-30 00:00:00
abstract::Cytomegaloviruses (CMVs) are ubiquitous pathogens known to employ numerous immunoevasive strategies that significantly impair the ability of the immune system to eliminate the infected cells. Here, we report that the single mouse CMV (MCMV) protein, m154, downregulates multiple surface molecules involved in the activa...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50803
更新日期:2020-01-13 00:00:00
abstract::Clinical trials are currently underway to assess the efficacy of forniceal deep brain stimulation (DBS) for improvement of memory in Alzheimer's patients, and forniceal DBS has been shown to improve learning and memory in a mouse model of Rett syndrome (RTT), an intellectual disability disorder caused by loss-of-funct...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.34031
更新日期:2018-03-23 00:00:00
abstract::In 2015, as part of the Reproducibility Project: Cancer Biology, we published a Registered Report (Kandela et al., 2015) that described how we intended to replicate selected experiments from the paper "BET bromodomain inhibition as a therapeutic strategy to target c-Myc" (Delmore et al., 2011). Here we report the resu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21253
更新日期:2017-01-19 00:00:00
abstract::Systemic oxygen restriction (SOR) is prevalent in numerous clinical conditions, including chronic obstructive pulmonary disease (COPD), and is associated with increased susceptibility to viral infections. However, the influence of SOR on T cell immunity remains uncharacterized. Here we show the detrimental effect of h...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56612
更新日期:2020-11-23 00:00:00
abstract::Seed dormancy is a complex life history trait that determines the timing of germination and is crucial for local adaptation. Genetic studies of dormancy are challenging, because the trait is highly plastic and strongly influenced by the maternal environment. Using a combination of statistical and experimental approach...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22502
更新日期:2016-12-14 00:00:00
abstract::To restore vision for the blind, several prosthetic approaches have been explored that convey raw images to the brain. So far, these schemes all suffer from a lack of bandwidth. An alternate approach would restore vision at the cognitive level, bypassing the need to convey sensory data. A wearable computer captures vi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.37841
更新日期:2018-11-27 00:00:00
abstract::Membrane transporters of the RND superfamily confer multidrug resistance to pathogenic bacteria, and are essential for cholesterol metabolism and embryonic development in humans. We use high-resolution X-ray crystallography and computational methods to delineate the mechanism of the homotrimeric RND-type proton/drug a...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03145
更新日期:2014-09-19 00:00:00
abstract::To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Bötzinger complex (preBötC) that putatively comprise the core respiratory rhythm genera...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03427
更新日期:2014-07-15 00:00:00
abstract::The NuRD complex subunit CHD4 is essential for fusion-positive rhabdomyosarcoma (FP-RMS) survival, but the mechanisms underlying this dependency are not understood. Here, a NuRD-specific CRISPR screen demonstrates that FP-RMS is particularly sensitive to CHD4 amongst the NuRD members. Mechanistically, NuRD complex con...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54993
更新日期:2020-08-03 00:00:00
abstract::Adhesion molecules hold cells together but also couple cell membranes to a contractile actomyosin network, which limits the expansion of cell contacts. Despite their fundamental role in tissue morphogenesis and tissue homeostasis, how adhesion molecules control cell shapes and cell patterns in tissues remains unclear....
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22796
更新日期:2017-05-24 00:00:00
abstract::TFIID-a complex of TATA-binding protein (TBP) and TBP-associated factors (TAFs)-is a central component of the Pol II promoter recognition apparatus. Recent studies have revealed significant downregulation of TFIID subunits in terminally differentiated myocytes, hepatocytes and adipocytes. Here, we report that TBP prot...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.08536
更新日期:2015-09-22 00:00:00
abstract::When a T cell and an antigen-presenting cell form an immunological synapse, rapid dynein-driven translocation of the centrosome toward the contact site leads to reorganization of microtubules and associated organelles. Currently, little is known about how the regulation of microtubule dynamics contributes to this proc...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.62876
更新日期:2020-12-21 00:00:00
abstract::Viral replicase recruitment and long-range RNA interactions are essential for RNA virus replication, yet the mechanism of their interplay remains elusive. Flaviviruses include numerous important human pathogens, e.g., dengue virus (DENV) and Zika virus (ZIKV). Here, we revealed a highly conserved, conformation-tunable...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17636
更新日期:2016-10-01 00:00:00
abstract::How circuits assemble starting from stem cells is a fundamental question in developmental neurobiology. We test the hypothesis that, in neuronal stem cells, temporal transcription factors predictably control neuronal terminal features and circuit assembly. Using the Drosophila motor system, we manipulate expression of...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46089
更新日期:2019-09-10 00:00:00
abstract::Dorsal/ventral (DV) patterning of the sea urchin embryo relies on a ventrally-localized organizer expressing Nodal, a pivotal regulator of the DV gene regulatory network. However, the inceptive mechanisms imposing the symmetry-breaking are incompletely understood. In Paracentrotus lividus, the Hbox12 homeodomain-conta...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.04664
更新日期:2014-12-02 00:00:00
abstract::Sensory deprivation during the post-natal 'critical period' leads to structural reorganization of the developing visual cortex. In adulthood, the visual cortex retains some flexibility and adapts to sensory deprivation. Here we show that short-term (2 hr) monocular deprivation in adult humans boosts the BOLD response ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.40014
更新日期:2018-11-26 00:00:00
abstract::Extracellular vesicles (EVs) encompass a variety of vesicles secreted into the extracellular space. EVs have been implicated in promoting tumor metastasis, but the molecular composition of tumor-derived EV sub-types and the mechanisms by which molecules are sorted into EVs remain mostly unknown. We report the separati...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47544
更新日期:2019-08-22 00:00:00
abstract::Positive-strand RNA viruses, the largest genetic class of viruses, include numerous important pathogens such as Zika virus. These viruses replicate their RNA genomes in novel, membrane-bounded mini-organelles, but the organization of viral proteins and RNAs in these compartments has been largely unknown. We used cryo-...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25940
更新日期:2017-06-27 00:00:00
abstract::Dendritic spines are specialized postsynaptic structures that transduce presynaptic signals, are regulated by neural activity and correlated with learning and memory. Most studies of spine function have focused on the mammalian nervous system. However, spine-like protrusions have been reported in C. elegans (Philbrook...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47918
更新日期:2019-10-04 00:00:00
abstract::The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.07420
更新日期:2015-07-31 00:00:00
abstract::WW domain tandem-containing proteins such as KIBRA, YAP, and MAGI play critical roles in cell growth and polarity via binding to and positioning target proteins in specific subcellular regions. An immense disparity exists between promiscuity of WW domain-mediated target bindings and specific roles of WW domain protein...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49439
更新日期:2019-09-05 00:00:00
abstract::Previous studies tracking AMPA receptor (AMPAR) diffusion at synapses observed a large mobile extrasynaptic AMPAR pool. Using super-resolution microscopy, we examined how fluorophore size and photostability affected AMPAR trafficking outside of, and within, post-synaptic densities (PSDs) from rats. Organic fluorescent...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.27744
更新日期:2017-07-27 00:00:00
abstract::Sleep affects brain activity globally, but many cortical sleep waves are spatially confined. Local rhythms serve cortical area-specific sleep needs and functions; however, mechanisms controlling locality are unclear. We identify the thalamic reticular nucleus (TRN) as a source for local, sensory-cortex-specific non-ra...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.39111
更新日期:2018-12-25 00:00:00