Family matters.

abstract：:In eukaryotes, intra-chromosomal recombination generates DNA circles, but little is known about how cells react to them. In yeast, partitioning of such circles to the mother cell at mitosis ensures their loss from the population but promotes replicative ageing. Nevertheless, the mechanisms of partitioning are debated....

journal_title：eLife

authors： Denoth-Lippuner A,Krzyzanowski MK,Stober C,Barral Y

更新日期：2014-11-17 00:00:00

abstract：:The enteric nervous system controls a variety of gastrointestinal functions including intestinal motility. The minimal neuronal circuit necessary to direct peristalsis is well-characterized but several intestinal regions display also other motility patterns for which the underlying circuits and connectivity schemes th...

journal_title：eLife

authors： Li Z,Hao MM,Van den Haute C,Baekelandt V,Boesmans W,Vanden Berghe P

更新日期：2019-02-12 00:00:00

abstract：:In bacteria, multicellular behaviors are regulated by cell-cell signaling through the exchange of both diffusible and contact-dependent signals. In a multicellular context, Myxococcus cells can share outer membrane (OM) materials by an unknown mechanism involving the traAB genes and gliding motility. Using live imagin...

journal_title：eLife

authors： Ducret A,Fleuchot B,Bergam P,Mignot T

更新日期：2013-07-23 00:00:00

abstract：:Host-virus arms races are inherently asymmetric; viruses evolve much more rapidly than host genomes. Thus, there is high interest in discovering mechanisms by which host genomes keep pace with rapidly evolving viruses. One family of restriction factors, the APOBEC3 (A3) cytidine deaminases, has undergone positive sele...

journal_title：eLife

authors： Yang L,Emerman M,Malik HS,McLaughlin RN Jnr

更新日期：2020-06-01 00:00:00

abstract：:Antiviral development is plagued by drug resistance and genetic barriers to resistance are needed. For HIV and hepatitis C virus (HCV), combination therapy has proved life-saving. The targets of direct-acting antivirals for HCV infection are NS3/4A protease, NS5A phosphoprotein and NS5B polymerase. Differential visual...

journal_title：eLife

authors： van Buuren N,Tellinghuisen TL,Richardson CD,Kirkegaard K

更新日期：2018-03-28 00:00:00

abstract：:To elucidate new functions of sphingosine (Sph), we demonstrate that the spontaneous elevation of intracellular Sph levels via caged Sph leads to a significant and transient calcium release from acidic stores that is independent of sphingosine 1-phosphate, extracellular and ER calcium levels. This photo-induced Sph-dr...

journal_title：eLife

authors： Höglinger D,Haberkant P,Aguilera-Romero A,Riezman H,Porter FD,Platt FM,Galione A,Schultz C

更新日期：2015-11-27 00:00:00

abstract：:In Arabidopsis thaliana, winter is registered during vernalization through the temperature-dependent repression and epigenetic silencing of floral repressor FLOWERING LOCUS C (FLC). Natural Arabidopsis accessions show considerable variation in vernalization. However, which aspect of the FLC repression mechanism is mos...

journal_title：eLife

authors： Hepworth J,Antoniou-Kourounioti RL,Berggren K,Selga C,Tudor EH,Yates B,Cox D,Collier Harris BR,Irwin JA,Howard M,Säll T,Holm S,Dean C

更新日期：2020-09-09 00:00:00

abstract：:Two recently discovered transcription factors stop cells from dividing when plants face extreme heat and DNA damage. ...

journal_title：eLife

authors： Eekhout T,De Veylder L

更新日期：2019-04-09 00:00:00

abstract：:The Escherichia coli DNA replication machinery has been used as a road map to uncover design rules that enable DNA duplication with high efficiency and fidelity. Although the enzymatic activities of the replicative DNA Pol III are well understood, its dynamics within the replisome are not. Here, we test the accepted v...

journal_title：eLife

authors： Lewis JS,Spenkelink LM,Jergic S,Wood EA,Monachino E,Horan NP,Duderstadt KE,Cox MM,Robinson A,Dixon NE,van Oijen AM

更新日期：2017-04-22 00:00:00

abstract：:The spliceosome is a complex machine composed of small nuclear ribonucleoproteins (snRNPs) and accessory proteins that excises introns from pre-mRNAs. After assembly the spliceosome is activated for catalysis by rearrangement of subunits to form an active site. How this rearrangement is coordinated is not well-underst...

journal_title：eLife

authors： Hoskins AA,Rodgers ML,Friedman LJ,Gelles J,Moore MJ

更新日期：2016-05-31 00:00:00

abstract：:The anterior cingulate cortex (ACC) encodes information supporting mnemonic and cognitive processes. We show here that a rat's position can be decoded with high spatiotemporal resolution from ACC activity. ACC neurons encoded the current state of the animal and task, except for brief excursions that sometimes occurred...

journal_title：eLife

authors： Mashhoori A,Hashemnia S,McNaughton BL,Euston DR,Gruber AJ

更新日期：2018-04-17 00:00:00

abstract：:The rhythm generating network for breathing must continuously adjust to changing metabolic and behavioral demands. Here, we examined network-based mechanisms in the mouse preBötzinger complex using substance P, a potent excitatory modulator of breathing frequency and stability, as a tool to dissect network properties ...

journal_title：eLife

authors： Baertsch NA,Ramirez JM

更新日期：2019-12-05 00:00:00

abstract：:Rhythmic actions benefit from synchronization with external events. Auditory-paced finger tapping studies indicate the two cerebral hemispheres preferentially control different rhythms. It is unclear whether left-lateralized processing of faster rhythms and right-lateralized processing of slower rhythms bases upon hem...

journal_title：eLife

authors： Pflug A,Gompf F,Muthuraman M,Groppa S,Kell CA

更新日期：2019-11-07 00:00:00

abstract：:A protein called P36 holds the key to how different species of malaria parasite invade liver cells. ...

journal_title：eLife

authors： Billker O

更新日期：2017-06-14 00:00:00

abstract：:A temperature-sensitive receptor prevents mosquitoes from being attracted to targets that are hotter than a potential host. ...

journal_title：eLife

authors： Greppi C,Budelli G,Garrity PA

更新日期：2015-12-15 00:00:00

abstract：:Drosophila larvae and adults possess a potent innate immune response, but the response of Drosophila eggs is poor. In contrast to Drosophila, eggs of the beetle Tribolium are protected by a serosa, an extraembryonic epithelium that is present in all insects except higher flies. In this study, we test a possible immune...

journal_title：eLife

authors： Jacobs CG,Spaink HP,van der Zee M

更新日期：2014-12-09 00:00:00

abstract：:Microtubules are nucleated from specific locations at precise times in the cell cycle. However, the factors that constitute these microtubule nucleation pathways and their mode of action still need to be identified. Using purified Xenopus laevis proteins we biochemically reconstitute branching microtubule nucleation, ...

journal_title：eLife

authors： Alfaro-Aco R,Thawani A,Petry S

更新日期：2020-01-14 00:00:00

abstract：:TMEM16F is activated by elevated intracellular Ca2+, and functions as a small-conductance ion channel and as a phospholipid scramblase. In contrast to its paralogs, the TMEM16A/B calcium-activated chloride channels, mouse TMEM16F has been reported as a cation-, anion-, or non-selective ion channel, without a definite ...

journal_title：eLife

authors： Ye W,Han TW,He M,Jan YN,Jan LY

更新日期：2019-07-18 00:00:00

abstract：:Postsynaptic compartments can be specifically modulated during various forms of synaptic plasticity, but it is unclear whether this precision is shared at presynaptic terminals. Presynaptic homeostatic plasticity (PHP) stabilizes neurotransmission at the Drosophila neuromuscular junction, where a retrograde enhancemen...

journal_title：eLife

authors： Li X,Goel P,Chen C,Angajala V,Chen X,Dickman DK

更新日期：2018-04-05 00:00:00

abstract：:In hypoxic stress conditions, glycolysis enzymes assemble into singular cytoplasmic granules called glycolytic (G) bodies. G body formation in yeast correlates with increased glucose consumption and cell survival. However, the physical properties and organizing principles that define G body formation are unclear. We d...

journal_title：eLife

authors： Fuller GG,Han T,Freeberg MA,Moresco JJ,Ghanbari Niaki A,Roach NP,Yates JR 3rd,Myong S,Kim JK

更新日期：2020-04-16 00:00:00

abstract：:Dendritic spines are specialized postsynaptic structures that transduce presynaptic signals, are regulated by neural activity and correlated with learning and memory. Most studies of spine function have focused on the mammalian nervous system. However, spine-like protrusions have been reported in C. elegans (Philbrook...

journal_title：eLife

authors： Cuentas-Condori A,Mulcahy B,He S,Palumbos S,Zhen M,Miller DM 3rd

更新日期：2019-10-04 00:00:00

abstract：:RNA-protein (RNP) granules have been proposed to assemble by forming solid RNA/protein aggregates or through phase separation into a liquid RNA/protein phase. Which model describes RNP granules in living cells is still unclear. In this study, we analyze P bodies in budding yeast and find that they have liquid-like pro...

journal_title：eLife

authors： Kroschwald S,Maharana S,Mateju D,Malinovska L,Nüske E,Poser I,Richter D,Alberti S

更新日期：2015-08-04 00:00:00

abstract：:Protein aggregation appears to originate from partially unfolded conformations that are sampled through stochastic fluctuations of the native protein. It has been a challenge to characterize these fluctuations, under native like conditions. Here, the conformational dynamics of the full-length (23-231) mouse prion prot...

journal_title：eLife

authors： Goluguri RR,Sen S,Udgaonkar J

更新日期：2019-04-26 00:00:00

abstract：:Microtubule dynamics facilitate neurite growth and establish morphology, but the role of minus-end binding proteins in these processes is largely unexplored. CAMSAP homologs associate with microtubule minus-ends, and are important for the stability of epithelial cell adhesions. In this study, we report morphological d...

journal_title：eLife

authors： Marcette JD,Chen JJ,Nonet ML

更新日期：2014-02-25 00:00:00

abstract：:Cell growth and/or proliferation may require the reprogramming of metabolic pathways, whereby a switch from oxidative to glycolytic metabolism diverts glycolytic intermediates towards anabolic pathways. Herein, we identify a novel role for TRIM32 in the maintenance of glycolytic flux mediated by biochemical interactio...

journal_title：eLife

authors： Bawa S,Brooks DS,Neville KE,Tipping M,Sagar MA,Kollhoff JA,Chawla G,Geisbrecht BV,Tennessen JM,Eliceiri KW,Geisbrecht ER

更新日期：2020-03-30 00:00:00

abstract：:Individuals must predict future events to proactively guide their behavior. Predicting when events will occur is a critical component of these expectations. Temporal expectations are often generated based on individual cue-duration relationships. However, the durations associated with different environmental cues will...

journal_title：eLife

authors： De Corte BJ,Della Valle RR,Matell MS

更新日期：2018-11-02 00:00:00

abstract：:Prion diseases are caused by PrPSc, a self-replicating pathologically misfolded protein that exerts toxicity predominantly in the brain. The administration of PrPSc causes a robust, reproducible and specific disease manifestation. Here, we have applied a combination of translating ribosome affinity purification and ri...

journal_title：eLife

authors： Scheckel C,Imeri M,Schwarz P,Aguzzi A

更新日期：2020-09-22 00:00:00

abstract：:Hsp90 is a homodimeric ATP-dependent molecular chaperone that remodels its substrate 'client' proteins, facilitating their folding and activating them for biological function. Despite decades of research, the mechanism connecting ATP hydrolysis and chaperone function remains elusive. Particularly puzzling has been the...

journal_title：eLife

authors： Elnatan D,Betegon M,Liu Y,Ramelot T,Kennedy MA,Agard DA

更新日期：2017-07-25 00:00:00

abstract：:Probabilistic models of adaptive immune repertoire sequence distributions can be used to infer the expansion of immune cells in response to stimulus, differentiate genetic from environmental factors that determine repertoire sharing, and evaluate the suitability of various target immune sequences for stimulation via v...

journal_title：eLife

authors： Davidsen K,Olson BJ,DeWitt WS 3rd,Feng J,Harkins E,Bradley P,Matsen FA 4th

更新日期：2019-09-05 00:00:00

abstract：:In response to cell death signals, an active apoptosome is assembled from Apaf-1 and procaspase-9 (pc-9). Here we report a near atomic structure of the active human apoptosome determined by cryo-electron microscopy. The resulting model gives insights into cytochrome c binding, nucleotide exchange and conformational ch...

journal_title：eLife

authors： Cheng TC,Hong C,Akey IV,Yuan S,Akey CW

更新日期：2016-10-04 00:00:00