Single-molecule visualization of fast polymerase turnover in the bacterial replisome.

abstract：:Macrophage-mediated phagocytosis and cytokine production represent the front lines of resistance to bacterial invaders. A key feature of this pro-inflammatory response in mammals is the complex remodeling of cellular metabolism towards aerobic glycolysis. Although the function of bactericidal macrophages is highly con...

journal_title：eLife

authors： Krejčová G,Danielová A,Nedbalová P,Kazek M,Strych L,Chawla G,Tennessen JM,Lieskovská J,Jindra M,Doležal T,Bajgar A

更新日期：2019-10-14 00:00:00

abstract：:Direct reprogramming of fibroblasts to neurons induces widespread cellular and transcriptional reconfiguration. Here, we characterized global epigenomic changes during the direct reprogramming of mouse fibroblasts to neurons using whole-genome base-resolution DNA methylation (mC) sequencing. We found that the pioneer ...

journal_title：eLife

authors： Luo C,Lee QY,Wapinski O,Castanon R,Nery JR,Mall M,Kareta MS,Cullen SM,Goodell MA,Chang HY,Wernig M,Ecker JR

更新日期：2019-01-15 00:00:00

abstract：:Calcium is a critical second messenger in neurons that contributes to learning and memory, but how the coordination of action potentials of neuronal ensembles with the hippocampal local field potential (LFP) is reflected in dynamic calcium activity remains unclear. Here, we recorded hippocampal calcium activity with e...

journal_title：eLife

authors： Zhou H,Neville KR,Goldstein N,Kabu S,Kausar N,Ye R,Nguyen TT,Gelwan N,Hyman BT,Gomperts SN

更新日期：2019-03-07 00:00:00

abstract：:A heat exchange mechanism in the head of kissing bugs helps to prevent stress and regulate their temperature while they feed on warm blood. ...

journal_title：eLife

authors： Benoit JB,Denlinger DL

更新日期：2017-11-21 00:00:00

abstract：:We generated induced excitatory neurons (iNeurons, iNs) from chimpanzee, bonobo, and human stem cells by expressing the transcription factor neurogenin-2 (NGN2). Single-cell RNA sequencing showed that genes involved in dendrite and synapse development are expressed earlier during iNs maturation in the chimpanzee and b...

journal_title：eLife

authors： Schörnig M,Ju X,Fast L,Ebert S,Weigert A,Kanton S,Schaffer T,Nadif Kasri N,Treutlein B,Peter BM,Hevers W,Taverna E

更新日期：2021-01-20 00:00:00

abstract：:Mammalian lifespan differs by >100 fold, but the mechanisms associated with such longevity differences are not understood. Here, we conducted a study on primary skin fibroblasts isolated from 16 species of mammals and maintained under identical cell culture conditions. We developed a pipeline for obtaining species-spe...

journal_title：eLife

authors： Ma S,Upneja A,Galecki A,Tsai YM,Burant CF,Raskind S,Zhang Q,Zhang ZD,Seluanov A,Gorbunova V,Clish CB,Miller RA,Gladyshev VN

更新日期：2016-11-22 00:00:00

abstract：:Cerebral cortical architecture at birth encodes regionally differential dendritic arborization and synaptic formation. It underlies behavioral emergence of 2-year-olds. Brain changes in 0-2 years are most dynamic across the lifespan. Effective prediction of future behavior with brain microstructure at birth will revea...

journal_title：eLife

authors： Ouyang M,Peng Q,Jeon T,Heyne R,Chalak L,Huang H

更新日期：2020-12-22 00:00:00

abstract：:We wish to identify determinants of endothelial lineage. Murine embryonic stem cells (mESC) were fused with human endothelial cells in stable, non-dividing, heterokaryons. Using RNA-seq, it is possible to discriminate between human and mouse transcripts in these chimeric heterokaryons. We observed a temporal pattern o...

journal_title：eLife

authors： Wong WT,Matrone G,Tian X,Tomoiaga SA,Au KF,Meng S,Yamazoe S,Sieveking D,Chen K,Burns DM,Chen JK,Blau HM,Cooke JP

更新日期：2017-03-21 00:00:00

abstract：:Phenazines are natural bacterial antibiotics that can protect crops from disease. However, for most crops it is unknown which producers and specific phenazines are ecologically relevant, and whether phenazine biodegradation can counter their effects. To better understand their ecology, we developed and environmentally...

journal_title：eLife

authors： Dar D,Thomashow LS,Weller DM,Newman DK

更新日期：2020-09-15 00:00:00

abstract：:A mathematical model shows how the shape of early multicellular organisms may have helped cells evolve specialized roles. ...

journal_title：eLife

authors： Staps M,Tarnita C

更新日期：2020-11-03 00:00:00

abstract：:Although individuals of many species inexorably age, a number of observations established that the rate of aging is modulated in response to a variety of mild stresses. Here, we investigated how heat stress promotes longevity in yeast. We show that upon growth at higher temperature, yeast cells relax the retention of ...

journal_title：eLife

authors： Baldi S,Bolognesi A,Meinema AC,Barral Y

更新日期：2017-12-28 00:00:00

abstract：:Neurons form bona fide synapses with oligodendrocyte precursor cells (OPCs), but the circuit context of these neuron to OPC synapses remains incompletely understood. Using monosynaptically-restricted rabies virus tracing of OPC afferents, we identified extensive afferent synaptic inputs to OPCs residing in secondary m...

journal_title：eLife

authors： Mount CW,Yalçın B,Cunliffe-Koehler K,Sundaresh S,Monje M

更新日期：2019-10-18 00:00:00

abstract：:When a female fly mates it produces a hormone that increases the size of its midgut and enhances fat metabolism in order to provide the energy needed for reproduction. ...

journal_title：eLife

authors： Flatt T

更新日期：2015-07-28 00:00:00

abstract：:CpG islands are gene regulatory elements associated with the majority of mammalian promoters, yet how they regulate gene expression remains poorly understood. Here, we identify FBXL19 as a CpG island-binding protein in mouse embryonic stem (ES) cells and show that it associates with the CDK-Mediator complex. We discov...

journal_title：eLife

authors： Dimitrova E,Kondo T,Feldmann A,Nakayama M,Koseki Y,Konietzny R,Kessler BM,Koseki H,Klose RJ

更新日期：2018-05-29 00:00:00

abstract：:Establishing a link between the nonsense-mediated decay pathway and a gene associated with programmed cell death could explain why this pathway is essential in most, but not all, eukaryotes. ...

journal_title：eLife

authors： Saveanu C,Jacquier A

更新日期：2016-04-27 00:00:00

abstract：:Exhaustion of stem cells is a hallmark of aging. In the Drosophila testis, dedifferentiated germline stem cells (GSCs) derived from spermatogonia increase during lifespan, leading to the model that dedifferentiation counteracts the decline of GSCs in aged males. To test this, we blocked dedifferentiation by mis-expres...

journal_title：eLife

authors： Herrera SC,Bach EA

更新日期：2018-07-09 00:00:00

abstract：:Apical constriction is a widely utilized cell shape change linked to folding, bending and invagination of polarized epithelia. It remains unclear how apical constriction is regulated spatiotemporally during tissue invagination and how this cellular process contributes to tube formation in different developmental conte...

journal_title：eLife

authors： Chung S,Kim S,Andrew DJ

更新日期：2017-03-06 00:00:00

abstract：:Brain metabolism can profoundly influence neuronal excitability. Mice with genetic deletion or alteration of Bad (BCL-2 agonist of cell death) exhibit altered brain-cell fuel metabolism, accompanied by resistance to acutely induced epileptic seizures; this seizure protection is mediated by ATP-sensitive potassium (KAT...

journal_title：eLife

authors： Martínez-François JR,Fernández-Agüera MC,Nathwani N,Lahmann C,Burnham VL,Danial NN,Yellen G

更新日期：2018-01-25 00:00:00

abstract：:TMEM16F is activated by elevated intracellular Ca2+, and functions as a small-conductance ion channel and as a phospholipid scramblase. In contrast to its paralogs, the TMEM16A/B calcium-activated chloride channels, mouse TMEM16F has been reported as a cation-, anion-, or non-selective ion channel, without a definite ...

journal_title：eLife

authors： Ye W,Han TW,He M,Jan YN,Jan LY

更新日期：2019-07-18 00:00:00

abstract：:Dopamine (D2) receptors provide autoinhibitory feedback onto dopamine neurons through well-known interactions with voltage-gated calcium channels and G protein-coupled inwardly-rectifying potassium (GIRK) channels. Here, we reveal a third major effector involved in D2R modulation of dopaminergic neurons - the sodium l...

journal_title：eLife

authors： Philippart F,Khaliq ZM

更新日期：2018-12-17 00:00:00

abstract：:Synaptic adhesion molecules regulate synapse development and function. However, whether and how presynaptic adhesion molecules regulate postsynaptic NMDAR function remains largely unclear. Presynaptic LAR family receptor tyrosine phosphatases (LAR-RPTPs) regulate synapse development through mechanisms that include tra...

journal_title：eLife

authors： Kim K,Shin W,Kang M,Lee S,Kim D,Kang R,Jung Y,Cho Y,Yang E,Kim H,Bae YC,Kim E

更新日期：2020-03-06 00:00:00

abstract：:Cell-cell communication in multicellular organisms depends on the dynamic and reversible phosphorylation of protein tyrosine residues. The receptor-linked protein tyrosine phosphatases (RPTPs) receive cues from the extracellular environment and are well placed to influence cell signaling. However, the direct events do...

journal_title：eLife

authors： Fearnley GW,Young KA,Edgar JR,Antrobus R,Hay IM,Liang WC,Martinez-Martin N,Lin W,Deane JE,Sharpe HJ

更新日期：2019-03-29 00:00:00

abstract：:Glucocorticoid receptors (GR) have diverse functions relevant to maintenance of homeostasis and adaptation to environmental challenges. Understanding the importance of tissue-specific GR function in physiology and behavior has been hampered by near-ubiquitous localization in brain and body. Here we use CRISPR/Cas9 gen...

journal_title：eLife

authors： Scheimann JR,Moloney RD,Mahbod P,Morano RL,Fitzgerald M,Hoskins O,Packard BA,Cotella EM,Hu YC,Herman JP

更新日期：2019-07-22 00:00:00

abstract：:Oncogenic human papillomaviruses (HPV) are small DNA viruses that infect keratinocytes. After HPV binding to cell surface receptors, a cascade of molecular interactions mediates the infectious cellular internalization of virus particles. Aside from the virus itself, important molecular players involved in virus entry ...

journal_title：eLife

authors： Mikuličić S,Finke J,Boukhallouk F,Wüstenhagen E,Sons D,Homsi Y,Reiss K,Lang T,Florin L

更新日期：2019-05-20 00:00:00

abstract：:Cytoplasmic dyneins are motor proteins in the AAA+ superfamily that transport cellular cargos toward microtubule minus-ends. Recently, ciliobrevins were reported as selective cell-permeable inhibitors of cytoplasmic dyneins. As is often true for first-in-class inhibitors, the use of ciliobrevins has in part been limit...

journal_title：eLife

authors： Steinman JB,Santarossa CC,Miller RM,Yu LS,Serpinskaya AS,Furukawa H,Morimoto S,Tanaka Y,Nishitani M,Asano M,Zalyte R,Ondrus AE,Johnson AG,Ye F,Nachury MV,Fukase Y,Aso K,Foley MA,Gelfand VI,Chen JK,Carter AP,Kapo

更新日期：2017-05-19 00:00:00

abstract：:Circadian rhythms are biological oscillations that schedule daily changes in physiology. Outside the laboratory, circadian clocks do not generally free-run but are driven by daily cues whose timing varies with the seasons. The principles that determine how circadian clocks align to these external cycles are not well u...

journal_title：eLife

authors： Leypunskiy E,Lin J,Yoo H,Lee U,Dinner AR,Rust MJ

更新日期：2017-07-07 00:00:00

abstract：:Previous studies had shown that the integration of genome wide expression profiles, in metabolic tissues, with genetic and phenotypic variance, provided valuable insight into the underlying molecular mechanisms. We used RNA-Seq to characterize hypothalamic transcriptome in 99 inbred strains of mice from the Hybrid Mou...

journal_title：eLife

authors： Hasin-Brumshtein Y,Khan AH,Hormozdiari F,Pan C,Parks BW,Petyuk VA,Piehowski PD,Brümmer A,Pellegrini M,Xiao X,Eskin E,Smith RD,Lusis AJ,Smith DJ

更新日期：2016-09-13 00:00:00

abstract：:Centering and decentering of cellular components is essential for internal organization of cells and their ability to perform basic cellular functions such as division and motility. How cells achieve proper localization of their organelles is still not well-understood, especially in large cells such as oocytes. Here, ...

journal_title：eLife

authors： Ierushalmi N,Malik-Garbi M,Manhart A,Abu Shah E,Goode BL,Mogilner A,Keren K

更新日期：2020-04-21 00:00:00

abstract：:Transcription at individual genes in single cells is often pulsatile and stochastic. A key question emerges regarding how this behaviour contributes to tissue phenotype, but it has been a challenge to quantitatively analyse this in living cells over time, as opposed to studying snap-shots of gene expression state. We ...

journal_title：eLife

authors： Featherstone K,Hey K,Momiji H,McNamara AV,Patist AL,Woodburn J,Spiller DG,Christian HC,McNeilly AS,Mullins JJ,Finkenstädt BF,Rand DA,White MR,Davis JR

更新日期：2016-02-01 00:00:00

abstract：:Candida albicans hyphae can reach enormous lengths, precluding their internalization by phagocytes. Nevertheless, macrophages engulf a portion of the hypha, generating incompletely sealed tubular phagosomes. These frustrated phagosomes are stabilized by a thick cuff of F-actin that polymerizes in response to non-canon...

journal_title：eLife

authors： Maxson ME,Naj X,O'Meara TR,Plumb JD,Cowen LE,Grinstein S

更新日期：2018-03-19 00:00:00