Drosophila macrophages switch to aerobic glycolysis to mount effective antibacterial defense.

abstract：:The choroid, which provides vascular supply to the outer retina, demonstrates progressive degeneration in aging and age-related macular degeneration (AMD). However mechanisms that maintain or compromise choroidal homeostasis are obscure. We discovered that the ablation of choroidal macrophages via CSF1R blockade was a...

journal_title：eLife

authors： Yang X,Zhao L,Campos MM,Abu-Asab M,Ortolan D,Hotaling N,Bharti K,Wong WT

更新日期：2020-04-01 00:00:00

abstract：:Initiatives to improve research communication can benefit from listening to graduate students, postdocs and newly-independent group leaders. ...

journal_title：eLife

authors： Patterson M,Schekman R

更新日期：2018-03-27 00:00:00

abstract：:Tumor-initiating stem cells (SCs) exhibit distinct patterns of transcription factors and gene expression compared to healthy counterparts. Here, we show that dramatic shifts in large open-chromatin domain (super-enhancer) landscapes underlie these differences and reflect tumor microenvironment. By in vivo super-enhanc...

journal_title：eLife

authors： Yang H,Schramek D,Adam RC,Keyes BE,Wang P,Zheng D,Fuchs E

更新日期：2015-11-21 00:00:00

abstract：:Although individuals of many species inexorably age, a number of observations established that the rate of aging is modulated in response to a variety of mild stresses. Here, we investigated how heat stress promotes longevity in yeast. We show that upon growth at higher temperature, yeast cells relax the retention of ...

journal_title：eLife

authors： Baldi S,Bolognesi A,Meinema AC,Barral Y

更新日期：2017-12-28 00:00:00

abstract：:The new open-access journal eLife has launched, making its first content available in PubMed Central. In addition to publishing science of the highest quality, the journal aims to improve both the peer-review process and the presentation of new research results. ...

journal_title：eLife

authors： Schekman R,Patterson M,Watt F,Weigel D

更新日期：2012-10-15 00:00:00

abstract：:Complexin regulates spontaneous and activates Ca(2+)-triggered neurotransmitter release, yet the molecular mechanisms are still unclear. Here we performed single molecule fluorescence resonance energy transfer experiments and uncovered two conformations of complexin-1 bound to the ternary SNARE complex. In the cis con...

journal_title：eLife

authors： Choi UB,Zhao M,Zhang Y,Lai Y,Brunger AT

更新日期：2016-06-02 00:00:00

abstract：:Auditory processing depends upon inhibitory signaling by interneurons, even at its earliest stages in the ventral cochlear nucleus (VCN). Remarkably, to date only a single subtype of inhibitory neuron has been documented in the VCN, a projection neuron termed the D-stellate cell. With the use of a transgenic mouse lin...

journal_title：eLife

authors： Ngodup T,Romero GE,Trussell LO

更新日期：2020-11-03 00:00:00

abstract：:Evolution persistently undermines vector control programs through insecticide resistance. Here we propose a novel strategy which instead exploits evolution to generate and sustain new control tools. Effective spatial repellents are needed to keep vectors out of houses. Our approach generates such new repellents by com...

journal_title：eLife

authors： Lynch PA,Boots M

更新日期：2016-10-25 00:00:00

abstract：:hIAPP fibrils are associated with Type-II Diabetes, but the link of hIAPP structure to islet cell death remains elusive. Here we observe that hIAPP fibrils are cytotoxic to cultured pancreatic β-cells, leading us to determine the structure and cytotoxicity of protein segments composing the amyloid spine of hIAPP. Usin...

journal_title：eLife

authors： Krotee P,Rodriguez JA,Sawaya MR,Cascio D,Reyes FE,Shi D,Hattne J,Nannenga BL,Oskarsson ME,Philipp S,Griner S,Jiang L,Glabe CG,Westermark GT,Gonen T,Eisenberg DS

更新日期：2017-01-03 00:00:00

abstract：:The axolotl can regenerate multiple organs, including the brain. It remains, however, unclear whether neuronal diversity, intricate tissue architecture, and axonal connectivity can be regenerated; yet, this is critical for recovery of function and a central aim of cell replacement strategies in the mammalian central n...

journal_title：eLife

authors： Amamoto R,Huerta VG,Takahashi E,Dai G,Grant AK,Fu Z,Arlotta P

更新日期：2016-05-09 00:00:00

abstract：:Patterns of spatial positioning of individuals within microbial communities are often critical to community function. However, understanding patterning in natural communities is hampered by the multitude of cell-cell and cell-environment interactions as well as environmental variability. Here, through simulations and ...

journal_title：eLife

authors： Momeni B,Brileya KA,Fields MW,Shou W

更新日期：2013-01-22 00:00:00

abstract：:Numerous brain regions have been shown to have neural correlates of gradually accumulating evidence for decision-making, but the causal roles of these regions in decisions driven by accumulation of evidence have yet to be determined. Here, in rats performing an auditory evidence accumulation task, we inactivated the f...

journal_title：eLife

authors： Erlich JC,Brunton BW,Duan CA,Hanks TD,Brody CD

更新日期：2015-04-14 00:00:00

abstract：:Differentiation of cellular lineages is facilitated by asymmetric segregation of fate determinants between dividing cells. In budding yeast, various aging factors segregate to the aging (mother)-lineage, with poorly understood consequences. In this study, we show that yeast mother cells form a protein aggregate during...

journal_title：eLife

authors： Saarikangas J,Barral Y

更新日期：2015-11-06 00:00:00

abstract：:During embryogenesis, chromatin accessibility profiles control lineage-specific gene expression by modulating transcription, thus impacting multipotent progenitor states and subsequent fate choices. Subsets of cardiac and pharyngeal/head muscles share a common origin in the cardiopharyngeal mesoderm, but the chromatin...

journal_title：eLife

authors： Racioppi C,Wiechecki KA,Christiaen L

更新日期：2019-11-20 00:00:00

abstract：:C-type inactivation of potassium channels fine-tunes the electrical signaling in excitable cells through an internal timing mechanism that is mediated by a hydrogen bond network in the channels' selectively filter. Previously, we used nonsense suppression to highlight the role of the conserved Trp434-Asp447 indole hyd...

journal_title：eLife

authors： Lueck JD,Mackey AL,Infield DT,Galpin JD,Li J,Roux B,Ahern CA

更新日期：2016-10-06 00:00:00

abstract：:Cells use multiple feedback controls to regulate metabolism in response to nutrient and signaling inputs. However, feedback creates the potential for unstable network responses. We examined how concentrations of key metabolites and signaling pathways interact to maintain homeostasis in proliferating human cells, using...

journal_title：eLife

authors： Hung YP,Teragawa C,Kosaisawe N,Gillies TE,Pargett M,Minguet M,Distor K,Rocha-Gregg BL,Coloff JL,Keibler MA,Stephanopoulos G,Yellen G,Brugge JS,Albeck JG

更新日期：2017-12-14 00:00:00

abstract：:A pair of neurons is required for nematodes to be able to navigate using the Earth's magnetic field. ...

journal_title：eLife

authors： Rankin CH,Lin CH

更新日期：2015-08-05 00:00:00

abstract：:Many pathogens possess the capacity for sex through outcrossing, despite being able to reproduce also asexually and/or via selfing. Given that sex is assumed to come at a cost, these mixed reproductive strategies typical of pathogens have remained puzzling. While the ecological and evolutionary benefits of outcrossing...

journal_title：eLife

authors： Laine AL,Barrès B,Numminen E,Siren JP

更新日期：2019-06-18 00:00:00

abstract：:IQ scores are correlated with the morphology and activity of certain neurons in the human temporal cortex. ...

journal_title：eLife

authors： Miller EN,Sherwood CC

更新日期：2019-02-05 00:00:00

abstract：:Why does a first pregnancy after age 35 increase the risk of breast cancer, and what can be done to combat this? ...

journal_title：eLife

authors： Chakravarthi BV,Varambally S

更新日期：2013-12-31 00:00:00

abstract：:Perturbation of neuronal activity is key to understanding the brain's functional properties, however, intervention studies typically perturb neurons in a nonspecific manner. Recent optogenetics techniques have enabled patterned perturbations, in which specific patterns of activity can be invoked in identified target n...

journal_title：eLife

authors： Sadeh S,Clopath C

更新日期：2020-02-19 00:00:00

abstract：:Brain connectivity maps display a delicate balance between individual variation and stereotypy, suggesting the existence of dedicated mechanisms that simultaneously permit and limit individual variation. We show that during the development of the Drosophila central nervous system, mutual inhibition among groups of nei...

journal_title：eLife

authors： Langen M,Koch M,Yan J,De Geest N,Erfurth ML,Pfeiffer BD,Schmucker D,Moreau Y,Hassan BA

更新日期：2013-03-05 00:00:00

abstract：:Clustering of receptors associated with immunoreceptor tyrosine-based activation motifs (ITAMs) initiates the macrophage antimicrobial response. ITAM receptors engage Src-family tyrosine kinases (SFKs) to initiate phagocytosis and macrophage activation. Macrophages also encounter nonpathogenic molecules that cluster r...

journal_title：eLife

authors： Freedman TS,Tan YX,Skrzypczynska KM,Manz BN,Sjaastad FV,Goodridge HS,Lowell CA,Weiss A

更新日期：2015-10-30 00:00:00

abstract：:Evolutionary changes in transcription networks are an important source of diversity across species, yet the quantitative consequences of network evolution have rarely been studied. Here we consider the transcriptional 'rewiring' of the three GAL genes that encode the enzymes needed for cells to convert galactose to gl...

journal_title：eLife

authors： Dalal CK,Zuleta IA,Mitchell KF,Andes DR,El-Samad H,Johnson AD

更新日期：2016-09-10 00:00:00

abstract：:Probabilistic models of adaptive immune repertoire sequence distributions can be used to infer the expansion of immune cells in response to stimulus, differentiate genetic from environmental factors that determine repertoire sharing, and evaluate the suitability of various target immune sequences for stimulation via v...

journal_title：eLife

authors： Davidsen K,Olson BJ,DeWitt WS 3rd,Feng J,Harkins E,Bradley P,Matsen FA 4th

更新日期：2019-09-05 00:00:00

abstract：:Recent epidemiological and laboratory-based studies suggest that the anti-diabetic drug metformin prevents cancer progression. How metformin diminishes tumor growth is not fully understood. In this study, we report that in human cancer cells, metformin inhibits mitochondrial complex I (NADH dehydrogenase) activity and...

journal_title：eLife

authors： Wheaton WW,Weinberg SE,Hamanaka RB,Soberanes S,Sullivan LB,Anso E,Glasauer A,Dufour E,Mutlu GM,Budigner GS,Chandel NS

更新日期：2014-05-13 00:00:00

abstract：:Oncogenic human papillomaviruses (HPV) are small DNA viruses that infect keratinocytes. After HPV binding to cell surface receptors, a cascade of molecular interactions mediates the infectious cellular internalization of virus particles. Aside from the virus itself, important molecular players involved in virus entry ...

journal_title：eLife

authors： Mikuličić S,Finke J,Boukhallouk F,Wüstenhagen E,Sons D,Homsi Y,Reiss K,Lang T,Florin L

更新日期：2019-05-20 00:00:00

abstract：:Sleep oscillations in the neocortex and hippocampus are critical for the integration of new memories into stable generalized representations in neocortex. However, the role of the thalamus in this process is poorly understood. To determine the thalamic contribution to non-REM oscillations (sharp-wave ripples, SWRs; sl...

journal_title：eLife

authors： Varela C,Wilson MA

更新日期：2020-06-11 00:00:00

abstract：:Temporal patterning of neural progenitors leads to the sequential production of diverse neurons. To understand how extrinsic cues influence intrinsic temporal programs, we studied Drosophila mushroom body progenitors (neuroblasts) that sequentially produce only three neuronal types: γ, then α'β', followed by αβ. Oppos...

journal_title：eLife

authors： Rossi AM,Desplan C

更新日期：2020-07-06 00:00:00

abstract：:While traditional microbiological freshwater tests focus on the detection of specific bacterial indicator species, including pathogens, direct tracing of all aquatic DNA through metagenomics poses a profound alternative. Yet, in situ metagenomic water surveys face substantial challenges in cost and logistics. Here, we...

journal_title：eLife

authors： Urban L,Holzer A,Baronas JJ,Hall MB,Braeuninger-Weimer P,Scherm MJ,Kunz DJ,Perera SN,Martin-Herranz DE,Tipper ET,Salter SJ,Stammnitz MR

更新日期：2021-01-19 00:00:00