Abstract:
:hIAPP fibrils are associated with Type-II Diabetes, but the link of hIAPP structure to islet cell death remains elusive. Here we observe that hIAPP fibrils are cytotoxic to cultured pancreatic β-cells, leading us to determine the structure and cytotoxicity of protein segments composing the amyloid spine of hIAPP. Using the cryoEM method MicroED, we discover that one segment, 19-29 S20G, forms pairs of β-sheets mated by a dry interface that share structural features with and are similarly cytotoxic to full-length hIAPP fibrils. In contrast, a second segment, 15-25 WT, forms non-toxic labile β-sheets. These segments possess different structures and cytotoxic effects, however, both can seed full-length hIAPP, and cause hIAPP to take on the cytotoxic and structural features of that segment. These results suggest that protein segment structures represent polymorphs of their parent protein and that segment 19-29 S20G may serve as a model for the toxic spine of hIAPP.
journal_name
Elifejournal_title
eLifeauthors
Krotee P,Rodriguez JA,Sawaya MR,Cascio D,Reyes FE,Shi D,Hattne J,Nannenga BL,Oskarsson ME,Philipp S,Griner S,Jiang L,Glabe CG,Westermark GT,Gonen T,Eisenberg DSdoi
10.7554/eLife.19273subject
Has Abstractpub_date
2017-01-03 00:00:00issn
2050-084Xjournal_volume
6pub_type
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