相关文献
eLife文献大全abstract::We present a reanalysis of the stochastic model of organelle production and show that the equilibrium distributions for the organelle numbers predicted by this model can be readily calculated in three different scenarios. These three distributions can be identified as standard distributions, and the corresponding exac...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10167
更新日期:2015-12-22 00:00:00
abstract::The twin-arginine protein translocation system (Tat) transports folded proteins across the bacterial cytoplasmic membrane and the thylakoid membranes of plant chloroplasts. The Tat transporter is assembled from multiple copies of the membrane proteins TatA, TatB, and TatC. We combine sequence co-evolution analysis, mo...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20718
更新日期:2016-12-03 00:00:00
abstract::A strain of budding yeast that contains one large chromosome reveals how the telomere capping complex CST maintains linear but not circular chromosomes. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60150
更新日期:2020-08-05 00:00:00
abstract::Convenient, efficient and fast whole-brain delivery of transgenes presents a persistent experimental challenge in neuroscience. Recent advances demonstrate whole-brain gene delivery by retro-orbital injection of virus, but slow and sparse expression and the large injection volumes required make this approach cumbersom...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53639
更新日期:2020-05-18 00:00:00
abstract::Trypanosome parasites are hiding in human skin, a discovery that may undermine efforts to eliminate sleeping sickness by 2020. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.21506
更新日期:2016-10-14 00:00:00
abstract::We report the crystal structure of nuclear import receptor Importin-9 bound to its cargo, the histones H2A-H2B. Importin-9 wraps around the core, globular region of H2A-H2B to form an extensive interface. The nature of this interface coupled with quantitative analysis of deletion mutants of H2A-H2B suggests that the N...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.43630
更新日期:2019-03-11 00:00:00
abstract::We determined differential gene expression in response to high glucose in lymphoblastoid cell lines derived from matched individuals with type 1 diabetes with and without retinopathy. Those genes exhibiting the largest difference in glucose response were assessed for association with diabetic retinopathy in a genome-w...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59980
更新日期:2020-11-09 00:00:00
abstract::Bacillus subtilis can measure the activity of the enzymes that remodel the cell wall to ensure that the levels of activity are 'just right'. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54244
更新日期:2020-01-24 00:00:00
abstract::Recently, a small-molecule communication mechanism was discovered in a range of Bacillus-infecting bacteriophages, which these temperate phages use to inform their lysis-lysogeny decision. We present a mathematical model of the ecological and evolutionary dynamics of such viral communication, and show that a communica...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58410
更新日期:2021-01-18 00:00:00
abstract::How Mycobacterium tuberculosis (Mtb) rewires macrophage energy metabolism to facilitate survival is poorly characterized. Here, we used extracellular flux analysis to simultaneously measure the rates of glycolysis and respiration in real time. Mtb infection induced a quiescent energy phenotype in human monocyte-derive...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.39169
更新日期:2018-11-16 00:00:00
abstract::Spore killers in fungi are selfish genetic elements that distort Mendelian segregation in their favor. It remains unclear how many species harbor them and how diverse their mechanisms are. Here, we discover two spore killers from a natural isolate of the fission yeast Schizosaccharomyces pombe. Both killers belong to ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.26057
更新日期:2017-06-20 00:00:00
abstract::In Arabidopsis thaliana, winter is registered during vernalization through the temperature-dependent repression and epigenetic silencing of floral repressor FLOWERING LOCUS C (FLC). Natural Arabidopsis accessions show considerable variation in vernalization. However, which aspect of the FLC repression mechanism is mos...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57671
更新日期:2020-09-09 00:00:00
abstract::The actomyosin ring generates force to ingress the cytokinetic cleavage furrow in animal cells, yet its filament organization and the mechanism of contractility is not well understood. We quantified actin filament order in human cells using fluorescence polarization microscopy and found that cleavage furrow ingression...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30867
更新日期:2017-11-06 00:00:00
abstract::Asymmetric disassembly of the synaptonemal complex (SC) is crucial for proper meiotic chromosome segregation. However, the signaling mechanisms that directly regulate this process are poorly understood. Here we show that the mammalian Rho GEF homolog, ECT-2, functions through the conserved RAS/ERK MAP kinase signaling...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12039
更新日期:2016-02-27 00:00:00
abstract::Rhythmic events recruit neuronal activity in the basal ganglia and cerebellum, but their roles remain elusive. In monkeys attempting to detect a single omission of isochronous visual stimulus, we found that neurons in the caudate nucleus showed increased activity for each stimulus in sequence, while those in the cereb...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.48702
更新日期:2019-09-06 00:00:00
abstract::Plasma cells (PCs) are essential for protection from infection, and at the origin of incurable cancers. Current studies do not circumvent the limitations of removing PCs from their microenvironment and confound formation and maintenance. Also, the investigation of PC population dynamics has mostly relied on nucleotide...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59850
更新日期:2020-11-02 00:00:00
abstract::We tested the proposal that the C-terminal domain (CTD) of the AMPAR subunit GluA1 is required for LTP. We found that a knock-in mouse lacking the CTD of GluA1 expresses normal LTP and spatial memory, assayed by the Morris water maze. Our results support a model in which LTP generates synaptic slots, which capture pas...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58042
更新日期:2020-08-24 00:00:00
abstract::The factors that govern assembly of the gut microbiota are insufficiently understood. Here, we test the hypothesis that inter-individual microbiota variation can arise solely from differences in the order and timing by which the gut is colonized early in life. Experiments in which mice were inoculated in sequence eith...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.36521
更新日期:2018-09-18 00:00:00
abstract::Selective packaging of HIV-1 genomic RNA (gRNA) requires the presence of a cis-acting RNA element called the 'packaging signal' (Ψ). However, the mechanism by which Ψ promotes selective packaging of the gRNA is not well understood. We used fluorescence correlation spectroscopy and quenching data to monitor the binding...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.27055
更新日期:2017-07-20 00:00:00
abstract::Cognitive functions such as working memory (WM) are emergent properties of large-scale network interactions. Synchronisation of oscillatory activity might contribute to WM by enabling the coordination of long-range processes. However, causal evidence for the way oscillatory activity shapes network dynamics and behavio...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22001
更新日期:2017-03-14 00:00:00
abstract::Rising and more variable global temperatures pose a challenge for biodiversity, with reproduction and fertility being especially sensitive to heat. Here, we assessed the potential for thermal adaptation in sperm and egg function using Tribolium flour beetles, a warm-temperate-tropical insect model. Following temperatu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49452
更新日期:2019-10-01 00:00:00
abstract::Recent advances in single-cell transcriptomics techniques have opened the door to the study of gene regulatory networks (GRNs) at the single-cell level. Here, we studied the GRNs controlling the emergence of hematopoietic stem and progenitor cells from mouse embryonic endothelium using a combination of single-cell tra...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.29312
更新日期:2018-03-20 00:00:00
abstract:Background:Malaria elimination strategies require a thorough understanding of parasite transmission from human to mosquito. A clinical model to induce gametocytes to understand their dynamics and evaluate transmission-blocking interventions (TBI) is currently unavailable. Here, we explore the use of the well-establishe...
journal_title:eLife
pub_type: 杂志文章,随机对照试验
doi:10.7554/eLife.31549
更新日期:2018-02-27 00:00:00
abstract::Two distinct mechanisms for primordial germ cell (PGC) specification are observed within Bilatera: early determination by maternal factors or late induction by zygotic cues. Here we investigate the molecular basis for PGC specification in Nematostella, a representative pre-bilaterian animal where PGCs arise as paired ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54573
更新日期:2020-09-24 00:00:00
abstract::In addition to their role in desensitization and internalization of G protein-coupled receptors (GPCRs), β-arrestins are essential scaffolds linking GPCRs to Erk1/2 signaling. However, their role in GPCR-operated Erk1/2 activation differs between GPCRs and the underlying mechanism remains poorly characterized. Here, w...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23777
更新日期:2017-02-07 00:00:00
abstract::Bacterial flagella mediate host-microbe interactions through tissue tropism during colonization, as well as by activating immune responses. The flagellar shaft of some bacteria, including several human pathogens, is encased in a membranous sheath of unknown function. While it has been hypothesized that the sheath may ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01579
更新日期:2014-03-04 00:00:00
abstract::Autoinducers are small signaling molecules that mediate intercellular communication in microbial populations and trigger coordinated gene expression via 'quorum sensing'. Elucidating the mechanisms that control autoinducer production is, thus, pertinent to understanding collective microbial behavior, such as virulence...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25773
更新日期:2017-07-25 00:00:00
abstract::Mitochondria are dynamic organelles that must precisely control their protein composition according to cellular energy demand. Although nuclear-encoded mRNAs can be localized to the mitochondrial surface, the importance of this localization is unclear. As yeast switch to respiratory metabolism, there is an increase in...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57814
更新日期:2020-08-07 00:00:00
abstract::Comparing the brains of related species faces the challenges of establishing homologies whilst accommodating evolutionary specializations. Here we propose a general framework for understanding similarities and differences between the brains of primates. The approach uses white matter blueprints of the whole cortex bas...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.35237
更新日期:2018-05-11 00:00:00
abstract::Seed dormancy is a complex life history trait that determines the timing of germination and is crucial for local adaptation. Genetic studies of dormancy are challenging, because the trait is highly plastic and strongly influenced by the maternal environment. Using a combination of statistical and experimental approach...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22502
更新日期:2016-12-14 00:00:00