Complexin induces a conformational change at the membrane-proximal C-terminal end of the SNARE complex.

abstract：:New ages for flowstone, sediments and fossil bones from the Dinaledi Chamber are presented. We combined optically stimulated luminescence dating of sediments with U-Th and palaeomagnetic analyses of flowstones to establish that all sediments containing Homo naledi fossils can be allocated to a single stratigraphic ent...

journal_title：eLife

authors： Dirks PH,Roberts EM,Hilbert-Wolf H,Kramers JD,Hawks J,Dosseto A,Duval M,Elliott M,Evans M,Grün R,Hellstrom J,Herries AI,Joannes-Boyau R,Makhubela TV,Placzek CJ,Robbins J,Spandler C,Wiersma J,Woodhead J,Berger LR

更新日期：2017-05-09 00:00:00

abstract：:Cognitive functions such as working memory (WM) are emergent properties of large-scale network interactions. Synchronisation of oscillatory activity might contribute to WM by enabling the coordination of long-range processes. However, causal evidence for the way oscillatory activity shapes network dynamics and behavio...

journal_title：eLife

authors： Violante IR,Li LM,Carmichael DW,Lorenz R,Leech R,Hampshire A,Rothwell JC,Sharp DJ

更新日期：2017-03-14 00:00:00

abstract：:A spring-loaded mechanism can explain the activation process for a protein that has a crucial role in maintaining the genomic integrity of immature eggs cells. ...

journal_title：eLife

authors： Amelio I,Melino G

更新日期：2016-05-23 00:00:00

abstract：:Vesicle fusion is mediated by an assembly of SNARE proteins between opposing membranes, but it is unknown whether transmembrane domains (TMDs) of SNARE proteins serve mechanistic functions that go beyond passive anchoring of the force-generating SNAREpin to the fusing membranes. Here, we show that conformational flexi...

journal_title：eLife

authors： Dhara M,Yarzagaray A,Makke M,Schindeldecker B,Schwarz Y,Shaaban A,Sharma S,Böckmann RA,Lindau M,Mohrmann R,Bruns D

更新日期：2016-06-25 00:00:00

abstract：:Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher olfactory centre, the lateral horn (LH), is implicated in innate behaviour. However, our structural and functional understanding of the LH is scant, in large part due to a lack of sparse neurogenetic t...

journal_title：eLife

authors： Dolan MJ,Frechter S,Bates AS,Dan C,Huoviala P,Roberts RJ,Schlegel P,Dhawan S,Tabano R,Dionne H,Christoforou C,Close K,Sutcliffe B,Giuliani B,Li F,Costa M,Ihrke G,Meissner GW,Bock DD,Aso Y,Rubin GM,Jefferis GS

更新日期：2019-05-21 00:00:00

abstract：:Multi-modal MRI techniques have identified biomarkers that could help to predict whether someone will develop epilepsy. ...

journal_title：eLife

authors： Gröhn O,Sierra A

更新日期：2017-07-26 00:00:00

abstract：:Cancer prevention is predicted to result in more positive therapeutic outcomes than post-diagnostic interventions, and so may be a viable option for future personalized medicine. ...

journal_title：eLife

authors： Komarova NL

更新日期：2015-08-05 00:00:00

abstract：:Although the elongating ribosome is an efficient helicase, certain mRNA stem-loop structures are known to impede ribosome movement along mRNA and stimulate programmed ribosome frameshifting via mechanisms that are not well understood. Using biochemical and single-molecule Förster resonance energy transfer (smFRET) exp...

journal_title：eLife

authors： Bao C,Loerch S,Ling C,Korostelev AA,Grigorieff N,Ermolenko DN

更新日期：2020-05-19 00:00:00

abstract：:In animal-based biomedical research, both the sex and the age of the animals studied affect disease phenotypes by modifying their susceptibility, presentation and response to treatment. The accurate reporting of experimental methods and materials, including the sex and age of animals, is essential so that other resear...

journal_title：eLife

authors： Flórez-Vargas O,Brass A,Karystianis G,Bramhall M,Stevens R,Cruickshank S,Nenadic G

更新日期：2016-03-03 00:00:00

abstract：:Using FIB-SEM we report the entire synaptic connectome of glomerulus VA1v of the right antennal lobe in Drosophila melanogaster. Within the glomerulus we densely reconstructed all neurons, including hitherto elusive local interneurons. The fruitless-positive, sexually dimorphic VA1v included >11,140 presynaptic sites ...

journal_title：eLife

authors： Horne JA,Langille C,McLin S,Wiederman M,Lu Z,Xu CS,Plaza SM,Scheffer LK,Hess HF,Meinertzhagen IA

更新日期：2018-11-01 00:00:00

abstract：:Oligomeric assemblies of intraflagellar transport (IFT) particles build cilia through sequential recruitment and transport of ciliary cargo proteins within cilia. Here we present the 1.8 Å resolution crystal structure of the Chlamydomonas IFT-B protein IFT80, which reveals the architecture of two N-terminal β-propelle...

journal_title：eLife

authors： Taschner M,Lorentzen A,Mourão A,Collins T,Freke GM,Moulding D,Basquin J,Jenkins D,Lorentzen E

更新日期：2018-04-16 00:00:00

abstract：:Centrioles are microtubule-based organelles crucial for cell division, sensing and motility. In Caenorhabditis elegans, the onset of centriole formation requires notably the proteins SAS-5 and SAS-6, which have functional equivalents across eukaryotic evolution. Whereas the molecular architecture of SAS-6 and its role...

journal_title：eLife

authors： Rogala KB,Dynes NJ,Hatzopoulos GN,Yan J,Pong SK,Robinson CV,Deane CM,Gönczy P,Vakonakis I

更新日期：2015-05-29 00:00:00

abstract：:Accurate chromosome segregation relies on bioriented amphitelic attachments of chromosomes to microtubules of the mitotic spindle, in which sister chromatids are connected to opposite spindle poles. BUB-1 is a protein of the Spindle Assembly Checkpoint (SAC) that coordinates chromosome attachment with anaphase onset. ...

journal_title：eLife

authors： Edwards F,Maton G,Gareil N,Canman JC,Dumont J

更新日期：2018-12-14 00:00:00

abstract：:Single-molecule experiments reveal the dynamics of transcription through a nucleosome with single-base-pair accuracy. ...

journal_title：eLife

authors： de Jong BE,van Noort J

更新日期：2019-09-13 00:00:00

abstract：:HIV-1 infection cannot be cured because the virus persists as integrated proviral DNA in long-lived cells despite years of suppressive antiretroviral therapy (ART). In a previous paper (Zanini et al, 2015) we documented HIV-1 evolution in 10 untreated patients. Here we characterize establishment, turnover, and evoluti...

journal_title：eLife

authors： Brodin J,Zanini F,Thebo L,Lanz C,Bratt G,Neher RA,Albert J

更新日期：2016-11-15 00:00:00

abstract：:We present in vivo single-cell FRET measurements in the Escherichia coli chemotaxis system that reveal pervasive signaling variability, both across cells in isogenic populations and within individual cells over time. We quantify cell-to-cell variability of adaptation, ligand response, as well as steady-state output le...

journal_title：eLife

authors： Keegstra JM,Kamino K,Anquez F,Lazova MD,Emonet T,Shimizu TS

更新日期：2017-12-12 00:00:00

abstract：:The non-canonical Wnt/Ca2+ signaling pathway plays important roles in embryonic development, tissue formation and diseases. However, it is unclear how the Wnt ligand-stimulated, G protein-coupled receptor Frizzled activates phospholipases for calcium release. Here, we report that the zebrafish/human phosphatidylinosit...

journal_title：eLife

authors： Gong B,Shen W,Xiao W,Meng Y,Meng A,Jia S

更新日期：2017-05-02 00:00:00

abstract：:The IFNλ family of interferons controls the spread of viruses in the upper respiratory tract and transmission between mice. ...

journal_title：eLife

authors： Wack A

更新日期：2018-05-24 00:00:00

abstract：:Multiple studies have reported a male bias in incidence and/or prevalence of malaria infection in males compared to females. To test the hypothesis that sex-based differences in host-parasite interactions affect the epidemiology of malaria, we intensively followed Plasmodium falciparum infections in a cohort in a mala...

journal_title：eLife

authors： Briggs J,Teyssier N,Nankabirwa JI,Rek J,Jagannathan P,Arinaitwe E,Bousema T,Drakeley C,Murray M,Crawford E,Hathaway N,Staedke SG,Smith D,Rosenthal PJ,Kamya M,Dorsey G,Rodriguez-Barraquer I,Greenhouse B

更新日期：2020-10-27 00:00:00

abstract：:The speed at which a cell fate decision in nematode worms evolves is due to the number of genes that control the decision, rather than to a high mutation rate. ...

journal_title：eLife

authors： Yang B,Rifkin SA

更新日期：2020-10-14 00:00:00

abstract：:Contrary to dogma, evolutionarily young and dynamic genes can encode essential functions. We find that evolutionarily dynamic ZAD-ZNF genes, which encode the most abundant class of insect transcription factors, are more likely to encode essential functions in Drosophila melanogaster than ancient, conserved ZAD-ZNF gen...

journal_title：eLife

authors： Kasinathan B,Colmenares SU 3rd,McConnell H,Young JM,Karpen GH,Malik HS

更新日期：2020-11-10 00:00:00

abstract：:Repression of genes by Polycomb requires that PRC2 modifies their chromatin by trimethylating lysine 27 on histone H3 (H3K27me3). At transcriptionally active genes, di- and tri-methylated H3K36 inhibit PRC2. Here, the cryo-EM structure of PRC2 on dinucleosomes reveals how binding of its catalytic subunit EZH2 to nucle...

journal_title：eLife

authors： Finogenova K,Bonnet J,Poepsel S,Schäfer IB,Finkl K,Schmid K,Litz C,Strauss M,Benda C,Müller J

更新日期：2020-11-19 00:00:00

abstract：:Rhabdomyosarcoma (RMS) is a pediatric malignacy of muscle with myogenic regulatory transcription factors MYOD and MYF5 being expressed in this disease. Consensus in the field has been that expression of these factors likely reflects the target cell of transformation rather than being required for continued tumor growt...

journal_title：eLife

authors： Tenente IM,Hayes MN,Ignatius MS,McCarthy K,Yohe M,Sindiri S,Gryder B,Oliveira ML,Ramakrishnan A,Tang Q,Chen EY,Petur Nielsen G,Khan J,Langenau DM

更新日期：2017-01-12 00:00:00

abstract：:Engineered molecules based on human cytokines have potential uses in research and medicine. ...

journal_title：eLife

authors： Villarino A,O'Shea JJ

更新日期：2017-05-12 00:00:00

abstract：:The integrity of the nuclear membranes coupled to the selective barrier of nuclear pore complexes (NPCs) are essential for the segregation of nucleoplasm and cytoplasm. Mechanical membrane disruption or perturbation to NPC assembly triggers an ESCRT-dependent surveillance system that seals nuclear pores: how these por...

journal_title：eLife

authors： Thaller DJ,Allegretti M,Borah S,Ronchi P,Beck M,Lusk CP

更新日期：2019-04-03 00:00:00

abstract：:We previously showed in Drosophila melanogaster embryos that low-affinity Ultrabithorax (Ubx)-responsive shavenbaby (svb) enhancers drive expression using localized transcriptional environments and that active svb enhancers on different chromosomes tended to colocalize (Tsai et al., 2017). Here, we test the hypothesis...

journal_title：eLife

authors： Tsai A,Alves MR,Crocker J

更新日期：2019-07-11 00:00:00

abstract：:Larval recruitment, the transition of pelagic larvae into reef-associated juveniles, is a critical step for the resilience of marine fish populations but its molecular control is unknown. Here, we investigate whether thyroid-hormones (TH) and their receptors (TR) coordinate the larval recruitment of the coral-reef-fis...

journal_title：eLife

authors： Holzer G,Besson M,Lambert A,François L,Barth P,Gillet B,Hughes S,Piganeau G,Leulier F,Viriot L,Lecchini D,Laudet V

更新日期：2017-10-30 00:00:00

abstract：:Telomeres are nucleoprotein complexes at the ends of chromosomes and are indispensable for the protection and lengthening of terminal DNA. Despite the evolutionarily conserved roles of telomeres, the telomeric double-strand DNA (dsDNA)-binding proteins have evolved rapidly. Here, we identified double-strand telomeric ...

journal_title：eLife

authors： Yamamoto I,Zhang K,Zhang J,Vorontsov E,Shibuya H

更新日期：2021-01-21 00:00:00

abstract：:Canonical transient receptor potential channels (TRPC) are involved in receptor-operated and/or store-operated Ca2+ signaling. Inhibition of TRPCs by small molecules was shown to be promising in treating renal diseases. In cells, the channels are regulated by calmodulin (CaM). Molecular details of both CaM and drug bi...

journal_title：eLife

authors： Vinayagam D,Quentin D,Yu-Strzelczyk J,Sitsel O,Merino F,Stabrin M,Hofnagel O,Yu M,Ledeboer MW,Nagel G,Malojcic G,Raunser S

更新日期：2020-11-25 00:00:00

abstract：:Bacterial cells are encased in a peptidoglycan (PG) exoskeleton that protects them from osmotic lysis and specifies their distinct shapes. Cell wall hydrolases are required to enlarge this covalently closed macromolecule during growth, but how these autolytic enzymes are regulated remains poorly understood. Bacillus s...

journal_title：eLife

authors： Dobihal GS,Brunet YR,Flores-Kim J,Rudner DZ

更新日期：2019-12-06 00:00:00