C. elegans neurons have functional dendritic spines.

abstract：:B-cell receptors form ordered clusters to recruit kinases and exclude phosphatases. ...

journal_title：eLife

authors： Bálint Š,Dustin ML

更新日期：2017-03-30 00:00:00

abstract：:Although mRNAs are localized in the processes of excitatory neurons, it is still unclear whether interneurons also localize a large population of mRNAs. In addition, the variability in the localized mRNA population within and between cell types is unknown. Here we describe the unbiased transcriptomic characterization ...

journal_title：eLife

authors： Perez JD,Dieck ST,Alvarez-Castelao B,Tushev G,Chan IC,Schuman EM

更新日期：2021-01-06 00:00:00

abstract：:Cell fate decisions are critical for life, yet little is known about how their reliability is achieved when signals are noisy and fluctuating with time. In this study, we show that in budding yeast, the decision of cell cycle commitment (Start) is determined by the time integration of its triggering signal Cln3. We fu...

journal_title：eLife

authors： Liu X,Wang X,Yang X,Liu S,Jiang L,Qu Y,Hu L,Ouyang Q,Tang C

更新日期：2015-01-14 00:00:00

abstract：:In vivo, the primary molecular mechanotransductive events mechanically initiating cell differentiation remain unknown. Here we find the molecular stretching of the highly conserved Y654-β-catenin-D665-E-cadherin binding site as mechanically induced by tissue strain. It triggers the increase of accessibility of the Y65...

journal_title：eLife

authors： Röper JC,Mitrossilis D,Stirnemann G,Waharte F,Brito I,Fernandez-Sanchez ME,Baaden M,Salamero J,Farge E

更新日期：2018-07-19 00:00:00

abstract：:Mechanical force is a determinant of Notch signalling but the mechanism of force detection and its coupling to Notch are unclear. We propose a role for Piezo1 channels, which are mechanically-activated non-selective cation channels. In cultured microvascular endothelial cells, Piezo1 channel activation by either shear...

journal_title：eLife

authors： Caolo V,Debant M,Endesh N,Futers TS,Lichtenstein L,Bartoli F,Parsonage G,Jones EA,Beech DJ

更新日期：2020-06-02 00:00:00

abstract：:Tuberculosis disproportionately affects the Canadian Inuit. To address this, it is imperative we understand transmission dynamics in this population. We investigate whether 'deep' sequencing can provide additional resolution compared to standard sequencing, using a well-characterized outbreak from the Arctic (2011-201...

journal_title：eLife

authors： Lee RS,Proulx JF,McIntosh F,Behr MA,Hanage WP

更新日期：2020-02-04 00:00:00

abstract：:Axons span extreme distances and are subject to significant stretch deformations during limb movements or sudden head movements, especially during impacts. Yet, axon biomechanics, and its relation to the ultrastructure that allows axons to withstand mechanical stress, is poorly understood. Using a custom developed for...

journal_title：eLife

authors： Dubey S,Bhembre N,Bodas S,Veer S,Ghose A,Callan-Jones A,Pullarkat P

更新日期：2020-04-08 00:00:00

abstract：:Numerous brain regions have been shown to have neural correlates of gradually accumulating evidence for decision-making, but the causal roles of these regions in decisions driven by accumulation of evidence have yet to be determined. Here, in rats performing an auditory evidence accumulation task, we inactivated the f...

journal_title：eLife

authors： Erlich JC,Brunton BW,Duan CA,Hanks TD,Brody CD

更新日期：2015-04-14 00:00:00

abstract：:Dietary magnesium (Mg2+) supplementation can enhance memory in young and aged rats. Memory-enhancing capacity was largely ascribed to increases in hippocampal synaptic density and elevated expression of the NR2B subunit of the NMDA-type glutamate receptor. Here we show that Mg2+ feeding also enhances long-term memory ...

journal_title：eLife

authors： Wu Y,Funato Y,Meschi E,Jovanoski KD,Miki H,Waddell S

更新日期：2020-11-26 00:00:00

abstract：:Cortical plasticity is fundamental to motor recovery following cortical perturbation. However, it is still unclear how this plasticity is induced at a functional circuit level. Here, we investigated motor recovery and underlying neural plasticity upon optogenetic suppression of a cortical area for eye movement. Using ...

journal_title：eLife

authors： Sato TR,Itokazu T,Osaki H,Ohtake M,Yamamoto T,Sohya K,Maki T,Sato TK

更新日期：2019-11-05 00:00:00

abstract：:Pancreatic ATP-sensitive K+ channels (KATP) comprise four inward rectifier subunits (Kir6.2), each associated with a sulphonylurea receptor (SUR1). ATP/ADP binding to Kir6.2 shuts KATP. Mg-nucleotide binding to SUR1 stimulates KATP. In the absence of Mg2+, SUR1 increases the apparent affinity for nucleotide inhibition...

journal_title：eLife

authors： Usher SG,Ashcroft FM,Puljung MC

更新日期：2020-01-07 00:00:00

abstract：:Newly born cells either continue to proliferate or exit the cell division cycle. This decision involves delaying expression of Cyclin E that promotes DNA replication. ORC1, the Origin Recognition Complex (ORC) large subunit, is inherited into newly born cells after it binds to condensing chromosomes during the precedi...

journal_title：eLife

authors： Hossain M,Stillman B

更新日期：2016-07-26 00:00:00

abstract：:Interactions between membrane protein interfaces in lipid bilayers play an important role in membrane protein folding but quantification of the strength of these interactions has been challenging. Studying dimerization of ClC-type transporters offers a new approach to the problem, as individual subunits adopt a stable...

journal_title：eLife

authors： Chadda R,Krishnamani V,Mersch K,Wong J,Brimberry M,Chadda A,Kolmakova-Partensky L,Friedman LJ,Gelles J,Robertson JL

更新日期：2016-08-03 00:00:00

abstract：:Researchers have identified two genes-follistatin and activin-as having an important role in the ability of certain flatworms to identify wounds that require the production of new tissue. ...

journal_title：eLife

authors： Sánchez Alvarado A

更新日期：2013-09-10 00:00:00

abstract：:Dengue Virus (DENV), a flavivirus spread by mosquito vectors, can cause vascular leakage and hemorrhaging. However, the processes that underlie increased vascular permeability and pathological plasma leakage during viral hemorrhagic fevers are largely unknown. Mast cells (MCs) are activated in vivo during DENV infecti...

journal_title：eLife

authors： St John AL,Rathore AP,Raghavan B,Ng ML,Abraham SN

更新日期：2013-04-30 00:00:00

abstract：:Perforin-2 (MPEG1) is a pore-forming, antibacterial protein with broad-spectrum activity. Perforin-2 is expressed constitutively in phagocytes and inducibly in parenchymal, tissue-forming cells. In vitro, Perforin-2 prevents the intracellular replication and proliferation of bacterial pathogens in these cells. Perfori...

journal_title：eLife

authors： McCormack RM,de Armas LR,Shiratsuchi M,Fiorentino DG,Olsson ML,Lichtenheld MG,Morales A,Lyapichev K,Gonzalez LE,Strbo N,Sukumar N,Stojadinovic O,Plano GV,Munson GP,Tomic-Canic M,Kirsner RS,Russell DG,Podack ER

更新日期：2015-09-24 00:00:00

abstract：:Understanding genome to phenotype linkages has been greatly enabled by genomic sequencing. However, most genome analysis is typically confined to the nuclear genome. We conducted a metabolomic QTL analysis on a reciprocal RIL population structured to examine how variation in the organelle genomes affects phenotypic va...

journal_title：eLife

authors： Joseph B,Corwin JA,Li B,Atwell S,Kliebenstein DJ

更新日期：2013-10-08 00:00:00

abstract：:Mitochondria play important roles in cellular processes and disease, yet little is known about how the transcriptional regime of the mitochondrial genome varies across individuals and tissues. By analyzing >11,000 RNA-sequencing libraries across 36 tissue/cell types, we find considerable variation in mitochondrial-enc...

journal_title：eLife

authors： Ali AT,Boehme L,Carbajosa G,Seitan VC,Small KS,Hodgkinson A

更新日期：2019-02-18 00:00:00

abstract：:Squamous cell carcinomas occurring at transition zones are highly malignant tumors with poor prognosis. The identity of the cell population and the signaling pathways involved in the progression of transition zone squamous cell carcinoma are poorly understood, hence representing limited options for targeted therapies....

journal_title：eLife

authors： McCauley HA,Chevrier V,Birnbaum D,Guasch G

更新日期：2017-02-21 00:00:00

abstract：:The Maternal Embryonic Leucine Zipper Kinase (MELK) has been identified as a promising therapeutic target in multiple cancer types. MELK over-expression is associated with aggressive disease, and MELK has been implicated in numerous cancer-related processes, including chemotherapy resistance, stem cell renewal, and tu...

journal_title：eLife

authors： Giuliano CJ,Lin A,Smith JC,Palladino AC,Sheltzer JM

更新日期：2018-02-08 00:00:00

abstract：:Microbial fitness screens are a key technique in functional genomics. We present an all-in-one solution, pyphe, for automating and improving data analysis pipelines associated with large-scale fitness screens, including image acquisition and quantification, data normalisation, and statistical analysis. Pyphe is versat...

journal_title：eLife

authors： Kamrad S,Rodríguez-López M,Cotobal C,Correia-Melo C,Ralser M,Bähler J

更新日期：2020-06-16 00:00:00

abstract：:In all living organisms, ribosomes translating membrane proteins are targeted to membrane translocons early in translation, by the ubiquitous signal recognition particle (SRP) system. In eukaryotes, the SRP Alu domain arrests translation elongation of membrane proteins until targeting is complete. Curiously, however, ...

journal_title：eLife

authors： Fluman N,Navon S,Bibi E,Pilpel Y

更新日期：2014-08-18 00:00:00

abstract：:Early mouse development is accompanied by dynamic changes in chromatin modifications, including G9a-mediated histone H3 lysine 9 dimethylation (H3K9me2), which is essential for embryonic development. Here we show that genome-wide accumulation of H3K9me2 is crucial for postimplantation development, and coincides with r...

journal_title：eLife

authors： Zylicz JJ,Dietmann S,Günesdogan U,Hackett JA,Cougot D,Lee C,Surani MA

更新日期：2015-11-09 00:00:00

abstract：:Rapid diagnostic tests (RDTs) have transformed malaria diagnosis. The most prevalent P. falciparum RDTs detect histidine-rich protein 2 (PfHRP2). However, pfhrp2 gene deletions yielding false-negative RDTs, first reported in South America in 2010, have been confirmed in Africa and Asia. We developed a mathematical mod...

journal_title：eLife

authors： Watson OJ,Slater HC,Verity R,Parr JB,Mwandagalirwa MK,Tshefu A,Meshnick SR,Ghani AC

更新日期：2017-08-24 00:00:00

abstract：:Many marine animals, ranging from corals to fishes, synchronise reproduction to lunar cycles. In the annelid Platynereis dumerilii, this timing is orchestrated by an endogenous monthly (circalunar) clock entrained by moonlight. Whereas daily (circadian) clocks cause extensive transcriptomic and proteomic changes, the ...

journal_title：eLife

authors： Schenk S,Bannister SC,Sedlazeck FJ,Anrather D,Minh BQ,Bileck A,Hartl M,von Haeseler A,Gerner C,Raible F,Tessmar-Raible K

更新日期：2019-02-15 00:00:00

abstract：:Knowledge of the host factors required for norovirus replication has been hindered by the challenges associated with culturing human noroviruses. We have combined proteomic analysis of the viral translation and replication complexes with a CRISPR screen, to identify host factors required for norovirus infection. The c...

journal_title：eLife

authors： Hosmillo M,Lu J,McAllaster MR,Eaglesham JB,Wang X,Emmott E,Domingues P,Chaudhry Y,Fitzmaurice TJ,Tung MK,Panas MD,McInerney G,Locker N,Wilen CB,Goodfellow IG

更新日期：2019-08-12 00:00:00

abstract：:More than 1500 fossils from the Rising Star cave system in South Africa have been assigned to a new human species, Homo naledi, which displays a unique combination of primitive and derived traits throughout the skeleton. ...

journal_title：eLife

authors： Stringer C

更新日期：2015-09-10 00:00:00

abstract：:Congenital diaphragmatic hernia (CDH) is a severe birth defect. Wt1-null mouse embryos develop CDH but the mechanisms regulated by WT1 are unknown. We have generated a murine model with conditional deletion of WT1 in the lateral plate mesoderm, using the G2 enhancer of the Gata4 gene as a driver. 80% of G2-Gata4(Cre);...

journal_title：eLife

authors： Carmona R,Cañete A,Cano E,Ariza L,Rojas A,Muñoz-Chápuli R

更新日期：2016-09-19 00:00:00

abstract：:Khoomei is a unique singing style originating from the republic of Tuva in central Asia. Singers produce two pitches simultaneously: a booming low-frequency rumble alongside a hovering high-pitched whistle-like tone. The biomechanics of this biphonation are not well-understood. Here, we use sound analysis, dynamic mag...

journal_title：eLife

authors： Bergevin C,Narayan C,Williams J,Mhatre N,Steeves JK,Bernstein JG,Story B

更新日期：2020-02-17 00:00:00

abstract：:In eukaryotes, intra-chromosomal recombination generates DNA circles, but little is known about how cells react to them. In yeast, partitioning of such circles to the mother cell at mitosis ensures their loss from the population but promotes replicative ageing. Nevertheless, the mechanisms of partitioning are debated....

journal_title：eLife

authors： Denoth-Lippuner A,Krzyzanowski MK,Stober C,Barral Y

更新日期：2014-11-17 00:00:00