The dimerization equilibrium of a ClC Cl(-)/H(+) antiporter in lipid bilayers.

abstract：:Animals seek out relevant information by moving through a dynamic world, but sensory systems are usually studied under highly constrained and passive conditions that may not probe important dimensions of the neural code. Here, we explored neural coding in the barrel cortex of head-fixed mice that tracked walls with th...

journal_title：eLife

authors： Sofroniew NJ,Vlasov YA,Hires SA,Freeman J,Svoboda K

更新日期：2015-12-23 00:00:00

abstract：:Actin filaments assemble inside the nucleus in response to multiple cellular perturbations, including heat shock, protein misfolding, integrin engagement, and serum stimulation. We find that DNA damage also generates nuclear actin filaments-detectable by phalloidin and live-cell actin probes-with three characteristic ...

journal_title：eLife

authors： Belin BJ,Lee T,Mullins RD

更新日期：2015-08-19 00:00:00

abstract：:XMAP215/Stu2/Alp14 proteins accelerate microtubule plus-end polymerization by recruiting tubulins via arrays of tumor overexpressed gene (TOG) domains, yet their mechanism remains unknown. Here, we describe the biochemical and structural basis for TOG arrays in recruiting and polymerizing tubulins. Alp14 binds four tu...

journal_title：eLife

authors： Nithianantham S,Cook BD,Beans M,Guo F,Chang F,Al-Bassam J

更新日期：2018-11-13 00:00:00

abstract：:Human disease agents exist within complex environments that have underappreciated effects on transmission, especially for parasites with multi-host life cycles. We examined the impact of multiple host and parasite species on transmission of the human parasite Schistosoma mansoni in Kenya. We show S. mansoni is impacte...

journal_title：eLife

authors： Laidemitt MR,Anderson LC,Wearing HJ,Mutuku MW,Mkoji GM,Loker ES

更新日期：2019-12-17 00:00:00

abstract：:Generating recombinant monoclonal antibodies (R-mAbs) from mAb-producing hybridomas offers numerous advantages that increase the effectiveness, reproducibility, and transparent reporting of research. We report here the generation of a novel resource in the form of a library of recombinant R-mAbs validated for neurosci...

journal_title：eLife

authors： Andrews NP,Boeckman JX,Manning CF,Nguyen JT,Bechtold H,Dumitras C,Gong B,Nguyen K,van der List D,Murray KD,Engebrecht J,Trimmer JS

更新日期：2019-01-22 00:00:00

abstract：:AMPARs control fast synaptic communication between neurons and their function relies on auxiliary subunits, which importantly modulate channel properties. Although it has been suggested that AMPARs can bind to TARPs with variable stoichiometry, little is known about the effect that this stoichiometry exerts on certain...

journal_title：eLife

authors： Miguez-Cabello F,Sánchez-Fernández N,Yefimenko N,Gasull X,Gratacòs-Batlle E,Soto D

更新日期：2020-05-26 00:00:00

abstract：:Selective packaging of HIV-1 genomic RNA (gRNA) requires the presence of a cis-acting RNA element called the 'packaging signal' (Ψ). However, the mechanism by which Ψ promotes selective packaging of the gRNA is not well understood. We used fluorescence correlation spectroscopy and quenching data to monitor the binding...

journal_title：eLife

authors： Comas-Garcia M,Datta SA,Baker L,Varma R,Gudla PR,Rein A

更新日期：2017-07-20 00:00:00

abstract：:Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Here, we describe a set of resources to streamline reporter gene knock-ins in zebrafish and demonstrate the broader utility of the method in mammalian cells. Our approach uses short homology of 24-...

journal_title：eLife

authors： Wierson WA,Welker JM,Almeida MP,Mann CM,Webster DA,Torrie ME,Weiss TJ,Kambakam S,Vollbrecht MK,Lan M,McKeighan KC,Levey J,Ming Z,Wehmeier A,Mikelson CS,Haltom JA,Kwan KM,Chien CB,Balciunas D,Ekker SC,Clark KJ,We

更新日期：2020-05-15 00:00:00

abstract：:Cell lineages provide the framework for understanding how cell fates are decided during development. Describing cell lineages in most organisms is challenging; even a fruit fly larva has ~50,000 cells and a small mammal has >1 billion cells. Recently, the idea of applying CRISPR to induce mutations during development,...

journal_title：eLife

authors： Salvador-Martínez I,Grillo M,Averof M,Telford MJ

更新日期：2019-01-28 00:00:00

abstract：:CRISPR-Cas systems provide sequence-specific immunity against phages and mobile genetic elements using CRISPR-associated nucleases guided by short CRISPR RNAs (crRNAs). Type III systems exhibit a robust immune response that can lead to the extinction of a phage population, a feat coordinated by a multi-subunit effecto...

journal_title：eLife

authors： Chou-Zheng L,Hatoum-Aslan A

更新日期：2019-04-03 00:00:00

abstract：:The structure of fitness landscapes is critical for understanding adaptive protein evolution. Previous empirical studies on fitness landscapes were confined to either the neighborhood around the wild type sequence, involving mostly single and double mutants, or a combinatorially complete subgraph involving only two am...

journal_title：eLife

authors： Wu NC,Dai L,Olson CA,Lloyd-Smith JO,Sun R

更新日期：2016-07-08 00:00:00

abstract：:The germ line provides the cellular link between generations of multicellular organisms, its cells entering the meiotic cell cycle only once each generation. However, the mechanisms governing this initiation of meiosis remain poorly understood. Here, we examined cells undergoing meiotic initiation in mice, and we foun...

journal_title：eLife

authors： Kojima ML,de Rooij DG,Page DC

更新日期：2019-02-27 00:00:00

abstract：:Human SEMAPHORIN 5A (SEMA5A) is an autism susceptibility gene; however, its function in brain development is unknown. In this study, we show that mouse Sema5A negatively regulates synaptogenesis in early, developmentally born, hippocampal dentate granule cells (GCs). Sema5A is strongly expressed by GCs and regulates d...

journal_title：eLife

authors： Duan Y,Wang SH,Song J,Mironova Y,Ming GL,Kolodkin AL,Giger RJ

更新日期：2014-10-14 00:00:00

abstract：:Axons span extreme distances and are subject to significant stretch deformations during limb movements or sudden head movements, especially during impacts. Yet, axon biomechanics, and its relation to the ultrastructure that allows axons to withstand mechanical stress, is poorly understood. Using a custom developed for...

journal_title：eLife

authors： Dubey S,Bhembre N,Bodas S,Veer S,Ghose A,Callan-Jones A,Pullarkat P

更新日期：2020-04-08 00:00:00

abstract：:Negative regulators of adult neurogenesis are of particular interest as targets to enhance neuronal repair, but few have yet been identified. Planarians can regenerate their entire CNS using pluripotent adult stem cells, and this process is robustly regulated to ensure that new neurons are produced in proper abundance...

journal_title：eLife

authors： Karge A,Bonar NA,Wood S,Petersen CP

更新日期：2020-01-20 00:00:00

abstract：:TFIID-a complex of TATA-binding protein (TBP) and TBP-associated factors (TAFs)-is a central component of the Pol II promoter recognition apparatus. Recent studies have revealed significant downregulation of TFIID subunits in terminally differentiated myocytes, hepatocytes and adipocytes. Here, we report that TBP prot...

journal_title：eLife

authors： Li L,Martinez SS,Hu W,Liu Z,Tjian R

更新日期：2015-09-22 00:00:00

abstract：:A mathematical model shows how the shape of early multicellular organisms may have helped cells evolve specialized roles. ...

journal_title：eLife

authors： Staps M,Tarnita C

更新日期：2020-11-03 00:00:00

abstract：:The receptor protein Dectin-1 recognizes structures found on cancerous cells, and then triggers an anti-tumor immune response. ...

journal_title：eLife

authors： Okabe Y,Medzhitov R

更新日期：2014-09-24 00:00:00

abstract：:Bacterial flagella mediate host-microbe interactions through tissue tropism during colonization, as well as by activating immune responses. The flagellar shaft of some bacteria, including several human pathogens, is encased in a membranous sheath of unknown function. While it has been hypothesized that the sheath may ...

journal_title：eLife

authors： Brennan CA,Hunt JR,Kremer N,Krasity BC,Apicella MA,McFall-Ngai MJ,Ruby EG

更新日期：2014-03-04 00:00:00

abstract：:Cytoplasmic dyneins are motor proteins in the AAA+ superfamily that transport cellular cargos toward microtubule minus-ends. Recently, ciliobrevins were reported as selective cell-permeable inhibitors of cytoplasmic dyneins. As is often true for first-in-class inhibitors, the use of ciliobrevins has in part been limit...

journal_title：eLife

authors： Steinman JB,Santarossa CC,Miller RM,Yu LS,Serpinskaya AS,Furukawa H,Morimoto S,Tanaka Y,Nishitani M,Asano M,Zalyte R,Ondrus AE,Johnson AG,Ye F,Nachury MV,Fukase Y,Aso K,Foley MA,Gelfand VI,Chen JK,Carter AP,Kapo

更新日期：2017-05-19 00:00:00

abstract：:Comparing the brains of related species faces the challenges of establishing homologies whilst accommodating evolutionary specializations. Here we propose a general framework for understanding similarities and differences between the brains of primates. The approach uses white matter blueprints of the whole cortex bas...

journal_title：eLife

authors： Mars RB,Sotiropoulos SN,Passingham RE,Sallet J,Verhagen L,Khrapitchev AA,Sibson N,Jbabdi S

更新日期：2018-05-11 00:00:00

abstract：:The piriform cortex (PCx) receives direct input from the olfactory bulb (OB) and is the brain's main station for odor recognition and memory. The transformation of the odor code from OB to PCx is profound: mitral and tufted cells in olfactory glomeruli respond to individual odorant molecules, whereas pyramidal neurons...

journal_title：eLife

authors： Kumar A,Schiff O,Barkai E,Mel BW,Poleg-Polsky A,Schiller J

更新日期：2018-12-21 00:00:00

abstract：:In eukaryotes, intra-chromosomal recombination generates DNA circles, but little is known about how cells react to them. In yeast, partitioning of such circles to the mother cell at mitosis ensures their loss from the population but promotes replicative ageing. Nevertheless, the mechanisms of partitioning are debated....

journal_title：eLife

authors： Denoth-Lippuner A,Krzyzanowski MK,Stober C,Barral Y

更新日期：2014-11-17 00:00:00

abstract：:To efficiently navigate through the environment and avoid potential threats, an animal must quickly detect the motion of approaching objects. Current models of primate vision place the origins of this complex computation in the visual cortex. Here, we report that detection of approaching motion begins in the retina. S...

journal_title：eLife

authors： Appleby TR,Manookin MB

更新日期：2020-02-24 00:00:00

abstract：:Aedes aegypti transmit pathogenic arboviruses while the mosquito itself tolerates the infection. We examine a piRNA-based immunity that relies on the acquisition of viral derived cDNA (vDNA) and how this pathway discriminates between self and non-self. The piRNAs derived from these vDNAs are essential for virus contro...

journal_title：eLife

authors： Tassetto M,Kunitomi M,Whitfield ZJ,Dolan PT,Sánchez-Vargas I,Garcia-Knight M,Ribiero I,Chen T,Olson KE,Andino R

更新日期：2019-10-17 00:00:00

abstract：:Increasing evidence highlights the important roles of microRNAs in mediating p53's tumor suppression functions. Here, we report miR-139-5p as another new p53 microRNA target. p53 induced the transcription of miR-139-5p, which in turn suppressed the protein levels of phosphodiesterase 4D (PDE4D), an oncogenic protein i...

journal_title：eLife

authors： Cao B,Wang K,Liao JM,Zhou X,Liao P,Zeng SX,He M,Chen L,He Y,Li W,Lu H

更新日期：2016-07-07 00:00:00

abstract：:Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study sarcomerogenesis, we have generated a transcriptomics resource of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, most sarcomeric compon...

journal_title：eLife

authors： Spletter ML,Barz C,Yeroslaviz A,Zhang X,Lemke SB,Bonnard A,Brunner E,Cardone G,Basler K,Habermann BH,Schnorrer F

更新日期：2018-05-30 00:00:00

abstract：:Our knowledge about the repertoire of ribosomal RNA modifications and the enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 is responsible for depositing m5C at position C2381 on the 26S rRNA in Caenorhabditis elegans. Here, we show that NSUN-1 is writing the second k...

journal_title：eLife

authors： Heissenberger C,Rollins JA,Krammer TL,Nagelreiter F,Stocker I,Wacheul L,Shpylovyi A,Tav K,Snow S,Grillari J,Rogers AN,Lafontaine DLJ,Schosserer M

更新日期：2020-12-08 00:00:00

abstract：:Most funding agencies rely on peer review to evaluate grant applications and proposals, but research into the use of this process by funding agencies has been limited. Here we explore if two changes to the organization of peer review for proposals submitted to various funding actions by the European Union has an influ...

journal_title：eLife

authors： Pina DG,Buljan I,Hren D,Marušić A

更新日期：2021-01-13 00:00:00

abstract：:It is now clear that microglia and macrophages are present in brain tumors, but whether or how they affect initiation and development of tumors is not known. Exploiting the advantages of the zebrafish (Danio rerio) model, we showed that macrophages and microglia respond immediately upon oncogene activation in the brai...

journal_title：eLife

authors： Chia K,Mazzolini J,Mione M,Sieger D

更新日期：2018-02-21 00:00:00