Is it possible to reconstruct an accurate cell lineage using CRISPR recorders?

abstract：:Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher olfactory centre, the lateral horn (LH), is implicated in innate behaviour. However, our structural and functional understanding of the LH is scant, in large part due to a lack of sparse neurogenetic t...

journal_title：eLife

authors： Dolan MJ,Frechter S,Bates AS,Dan C,Huoviala P,Roberts RJ,Schlegel P,Dhawan S,Tabano R,Dionne H,Christoforou C,Close K,Sutcliffe B,Giuliani B,Li F,Costa M,Ihrke G,Meissner GW,Bock DD,Aso Y,Rubin GM,Jefferis GS

更新日期：2019-05-21 00:00:00

abstract：:Heterochromatin formed by the SUV39 histone methyltransferases represses transcription from repetitive DNA sequences and ensures genomic stability. How SUV39 enzymes localize to their target genomic loci remains unclear. Here, we demonstrate that chromatin-associated RNA contributes to the stable association of SUV39H...

journal_title：eLife

authors： Johnson WL,Yewdell WT,Bell JC,McNulty SM,Duda Z,O'Neill RJ,Sullivan BA,Straight AF

更新日期：2017-08-01 00:00:00

abstract：:Signals delivered by costimulatory molecules are implicated in driving T cell expansion. The requirements for these signals, however, vary from dispensable to essential in different infections. We examined the underlying mechanisms of this differential T cell costimulation dependence and found that the viral context d...

journal_title：eLife

authors： Welten SP,Redeker A,Franken KL,Oduro JD,Ossendorp F,Čičin-Šain L,Melief CJ,Aichele P,Arens R

更新日期：2015-08-11 00:00:00

abstract：:The new open-access journal eLife has launched, making its first content available in PubMed Central. In addition to publishing science of the highest quality, the journal aims to improve both the peer-review process and the presentation of new research results. ...

journal_title：eLife

authors： Schekman R,Patterson M,Watt F,Weigel D

更新日期：2012-10-15 00:00:00

abstract：:Gene copy number alterations, tumor cell stemness, and the development of platinum chemotherapy resistance contribute to high-grade serous ovarian cancer (HGSOC) recurrence. Stem phenotypes involving Wnt-β-catenin, aldehyde dehydrogenase activities, intrinsic platinum resistance, and tumorsphere formation are here ass...

journal_title：eLife

authors： Diaz Osterman CJ,Ozmadenci D,Kleinschmidt EG,Taylor KN,Barrie AM,Jiang S,Bean LM,Sulzmaier FJ,Jean C,Tancioni I,Anderson K,Uryu S,Cordasco EA,Li J,Chen XL,Fu G,Ojalill M,Rappu P,Heino J,Mark AM,Xu G,Fisch KM,K

更新日期：2019-09-03 00:00:00

abstract：:Myelin, made by oligodendrocytes, is essential for rapid information transfer in the central nervous system. Oligodendrocyte precursors (OPs) receive glutamatergic synaptic input from axons but how this affects their development is unclear. Murine OPs in white matter express AMPA receptor (AMPAR) subunits GluA2, GluA3...

journal_title：eLife

authors： Kougioumtzidou E,Shimizu T,Hamilton NB,Tohyama K,Sprengel R,Monyer H,Attwell D,Richardson WD

更新日期：2017-06-13 00:00:00

abstract：:Development of specialized cells and structures in the heart is regulated by spatially -restricted molecular pathways. Disruptions in these pathways can cause severe congenital cardiac malformations or functional defects. To better understand these pathways and how they regulate cardiac development we used tomo-seq, c...

journal_title：eLife

authors： Burkhard SB,Bakkers J

更新日期：2018-02-05 00:00:00

abstract：:Yes. ...

journal_title：eLife

authors： Goldstein RE

更新日期：2018-07-23 00:00:00

abstract：:The Cdc45/Mcm2-7/GINS (CMG) helicase separates DNA strands during replication in eukaryotes. How the CMG is assembled and engages DNA substrates remains unclear. Using electron microscopy, we have determined the structure of the CMG in the presence of ATPγS and a DNA duplex bearing a 3' single-stranded tail. The struc...

journal_title：eLife

authors： Costa A,Renault L,Swuec P,Petojevic T,Pesavento JJ,Ilves I,MacLellan-Gibson K,Fleck RA,Botchan MR,Berger JM

更新日期：2014-08-12 00:00:00

abstract：:The permeability barrier of nuclear pore complexes (NPCs) controls bulk nucleocytoplasmic exchange. It consists of nucleoporin domains rich in phenylalanine-glycine motifs (FG domains). As a bottom-up nanoscale model for the permeability barrier, we have used planar films produced with three different end-grafted FG d...

journal_title：eLife

authors： Zahn R,Osmanović D,Ehret S,Araya Callis C,Frey S,Stewart M,You C,Görlich D,Hoogenboom BW,Richter RP

更新日期：2016-04-08 00:00:00

abstract：:The CRISPR-Cas9 bacterial surveillance system has become a versatile tool for genome editing and gene regulation in eukaryotic cells, yet how CRISPR-Cas9 contends with the barriers presented by eukaryotic chromatin is poorly understood. Here we investigate how the smallest unit of chromatin, a nucleosome, constrains t...

journal_title：eLife

authors： Isaac RS,Jiang F,Doudna JA,Lim WA,Narlikar GJ,Almeida R

更新日期：2016-04-28 00:00:00

abstract：:High-altitude environments require that animals meet the metabolic O2 demands for locomotion and thermogenesis in O2-thin air, but the degree to which convergent metabolic changes have arisen across independent high-altitude lineages or the speed at which such changes arise is unclear. We examined seven high-altitude ...

journal_title：eLife

authors： Dawson NJ,Alza L,Nandal G,Scott GR,McCracken KG

更新日期：2020-07-30 00:00:00

abstract：:Synonymous codon usage (SCU) varies widely among human genes. In particular, genes involved in different functional categories display a distinct codon usage, which was interpreted as evidence that SCU is adaptively constrained to optimize translation efficiency in distinct cellular states. We demonstrate here that SC...

journal_title：eLife

authors： Pouyet F,Mouchiroud D,Duret L,Sémon M

更新日期：2017-08-15 00:00:00

abstract：:Glucocorticoid receptor (GR) is crucial for signaling mediated by stress-induced high levels of glucocorticoids. The lateral nucleus of the amygdala (LA) is a key structure underlying auditory-cued fear conditioning. Here, we demonstrate that genetic disruption of GR in the LA (LAGRKO) resulted in an auditory-cued fea...

journal_title：eLife

authors： Inoue R,Abdou K,Hayashi-Tanaka A,Muramatsu SI,Mino K,Inokuchi K,Mori H

更新日期：2018-06-26 00:00:00

abstract：:One of the major unanswered questions in evolutionary biology is when and how the transition between diderm (two membranes) and monoderm (one membrane) cell envelopes occurred in Bacteria. The Negativicutes and the Halanaerobiales belong to the classically monoderm Firmicutes, but possess outer membranes with lipopoly...

journal_title：eLife

authors： Antunes LC,Poppleton D,Klingl A,Criscuolo A,Dupuy B,Brochier-Armanet C,Beloin C,Gribaldo S

更新日期：2016-08-31 00:00:00

abstract：:Innate lymphoid cells (ILCs) contribute to host defence and tissue repair but can induce immunopathology. Recent work has revealed tissue-specific roles for ILCs; however, the question of how a small population has large effects on immune homeostasis remains unclear. We identify two mechanisms that ILC3s utilise to ex...

journal_title：eLife

authors： Pearson C,Thornton EE,McKenzie B,Schaupp AL,Huskens N,Griseri T,West N,Tung S,Seddon BP,Uhlig HH,Powrie F

更新日期：2016-01-18 00:00:00

abstract：:Recent epidemiological and laboratory-based studies suggest that the anti-diabetic drug metformin prevents cancer progression. How metformin diminishes tumor growth is not fully understood. In this study, we report that in human cancer cells, metformin inhibits mitochondrial complex I (NADH dehydrogenase) activity and...

journal_title：eLife

authors： Wheaton WW,Weinberg SE,Hamanaka RB,Soberanes S,Sullivan LB,Anso E,Glasauer A,Dufour E,Mutlu GM,Budigner GS,Chandel NS

更新日期：2014-05-13 00:00:00

abstract：:Multiple lines of evidence suggest that functionally intact cerebello-hippocampal interactions are required for appropriate spatial processing. However, how the cerebellum anatomically and physiologically engages with the hippocampus to sustain such communication remains unknown. Using rabies virus as a retrograde tra...

journal_title：eLife

authors： Watson TC,Obiang P,Torres-Herraez A,Watilliaux A,Coulon P,Rochefort C,Rondi-Reig L

更新日期：2019-06-17 00:00:00

abstract：:Plasma Membrane is the primary structure for adjusting to ever changing conditions. PM sub-compartmentalization in domains is thought to orchestrate signaling. Yet, mechanisms governing membrane organization are mostly uncharacterized. The plant-specific REMORINs are proteins regulating hormonal crosstalk and host inv...

journal_title：eLife

authors： Gronnier J,Crowet JM,Habenstein B,Nasir MN,Bayle V,Hosy E,Platre MP,Gouguet P,Raffaele S,Martinez D,Grelard A,Loquet A,Simon-Plas F,Gerbeau-Pissot P,Der C,Bayer EM,Jaillais Y,Deleu M,Germain V,Lins L,Mongrand S

更新日期：2017-07-31 00:00:00

abstract：:Dyneins are motor proteins responsible for transport in the cytoplasm and the beating of axonemes in cilia and flagella. They bind and release microtubules via a compact microtubule-binding domain (MTBD) at the end of a coiled-coil stalk. We address how cytoplasmic and axonemal dynein MTBDs bind microtubules at near a...

journal_title：eLife

authors： Lacey SE,He S,Scheres SH,Carter AP

更新日期：2019-07-02 00:00:00

abstract：:Plasmodium falciparum causes malaria in humans with over 450,000 deaths annually. The asexual blood stage involves invasion of erythrocytes by merozoites, in which they grow and divide to release daughter merozoites, which in turn invade new erythrocytes perpetuating the cycle responsible for malaria. A key step in me...

journal_title：eLife

authors： Chen L,Xu Y,Wong W,Thompson JK,Healer J,Goddard-Borger ED,Lawrence MC,Cowman AF

更新日期：2017-02-14 00:00:00

abstract：:Variation in the presentation of hereditary immunodeficiencies may be explained by genetic or environmental factors. Patients with mutations in HOIL1 (RBCK1) present with amylopectinosis-associated myopathy with or without hyper-inflammation and immunodeficiency. We report that barrier-raised HOIL-1-deficient mice exh...

journal_title：eLife

authors： MacDuff DA,Reese TA,Kimmey JM,Weiss LA,Song C,Zhang X,Kambal A,Duan E,Carrero JA,Boisson B,Laplantine E,Israel A,Picard C,Colonna M,Edelson BT,Sibley LD,Stallings CL,Casanova JL,Iwai K,Virgin HW

更新日期：2015-01-20 00:00:00

abstract：:The prefrontal cortex (PFC)'s functions are thought to include working memory, as its activity can reflect information that must be temporarily maintained to realize the current goal. We designed a flexible spatial working memory task that required rats to navigate - after distractions and a delay - to multiple possib...

journal_title：eLife

authors： Böhm C,Lee AK

更新日期：2020-12-24 00:00:00

abstract：:Mitogen-activated protein kinases (MAPK) such as p38 and the c-Jun N-terminal kinases (JNKs) are activated during the cellular response to stress signals. Their activity is regulated by the MAPK-phosphatase 1 (DUSP1), a key component of the anti-inflammatory response. Stress kinases are well-described elements of the ...

journal_title：eLife

authors： Celaya AM,Sánchez-Pérez I,Bermúdez-Muñoz JM,Rodríguez-de la Rosa L,Pintado-Berninches L,Perona R,Murillo-Cuesta S,Varela-Nieto I

更新日期：2019-04-02 00:00:00

abstract：:Improving the peer review process, overcoming the limitations of print journals and providing open access to the very best work in the life and biomedical sciences are three highlights of our first year. ...

journal_title：eLife

authors： Schekman R,Watt FM,Weigel D

更新日期：2013-10-15 00:00:00

abstract：:In animal-based biomedical research, both the sex and the age of the animals studied affect disease phenotypes by modifying their susceptibility, presentation and response to treatment. The accurate reporting of experimental methods and materials, including the sex and age of animals, is essential so that other resear...

journal_title：eLife

authors： Flórez-Vargas O,Brass A,Karystianis G,Bramhall M,Stevens R,Cruickshank S,Nenadic G

更新日期：2016-03-03 00:00:00

abstract：:Pattern completion, or the ability to retrieve stable neural activity patterns from noisy or partial cues, is a fundamental feature of memory. Theoretical studies indicate that recurrently connected auto-associative or discrete attractor networks can perform this process. Although pattern completion and attractor dyna...

journal_title：eLife

authors： Bolding KA,Nagappan S,Han BX,Wang F,Franks KM

更新日期：2020-07-14 00:00:00

abstract：:The link between the combined action of neuromodulators in the brain and global brain states remains a mystery. In this study, using biophysically realistic models of the thalamocortical network, we identified the critical intrinsic and synaptic mechanisms, associated with the putative action of acetylcholine (ACh), G...

journal_title：eLife

authors： Krishnan GP,Chauvette S,Shamie I,Soltani S,Timofeev I,Cash SS,Halgren E,Bazhenov M

更新日期：2016-11-16 00:00:00

abstract：:Previous studies had shown that the integration of genome wide expression profiles, in metabolic tissues, with genetic and phenotypic variance, provided valuable insight into the underlying molecular mechanisms. We used RNA-Seq to characterize hypothalamic transcriptome in 99 inbred strains of mice from the Hybrid Mou...

journal_title：eLife

authors： Hasin-Brumshtein Y,Khan AH,Hormozdiari F,Pan C,Parks BW,Petyuk VA,Piehowski PD,Brümmer A,Pellegrini M,Xiao X,Eskin E,Smith RD,Lusis AJ,Smith DJ

更新日期：2016-09-13 00:00:00

abstract：:Activation of systemic acquired resistance in plants is associated with transcriptome reprogramming induced by the unstable coactivator NPR1. Immune-induced ubiquitination and proteasomal degradation of NPR1 are thought to facilitate continuous delivery of active NPR1 to target promoters, thereby maximising gene expre...

journal_title：eLife

authors： Skelly MJ,Furniss JJ,Grey H,Wong KW,Spoel SH

更新日期：2019-10-07 00:00:00