A resource for the Drosophila antennal lobe provided by the connectome of glomerulus VA1v.

Abstract:

:Using FIB-SEM we report the entire synaptic connectome of glomerulus VA1v of the right antennal lobe in Drosophila melanogaster. Within the glomerulus we densely reconstructed all neurons, including hitherto elusive local interneurons. The fruitless-positive, sexually dimorphic VA1v included >11,140 presynaptic sites with ~38,050 postsynaptic dendrites. These connected input olfactory receptor neurons (ORNs, 51 ipsilateral, 56 contralateral), output projection neurons (18 PNs), and local interneurons (56 of >150 previously reported LNs). ORNs are predominantly presynaptic and PNs predominantly postsynaptic; newly reported LN circuits are largely an equal mixture and confer extensive synaptic reciprocity, except the newly reported LN2V with input from ORNs and outputs mostly to monoglomerular PNs, however. PNs were more numerous than previously reported from genetic screens, suggesting that the latter failed to reach saturation. We report a matrix of 192 bodies each having >50 connections; these form 88% of the glomerulus' pre/postsynaptic sites.

journal_name

Elife

journal_title

eLife

authors

Horne JA,Langille C,McLin S,Wiederman M,Lu Z,Xu CS,Plaza SM,Scheffer LK,Hess HF,Meinertzhagen IA

doi

10.7554/eLife.37550

subject

Has Abstract

pub_date

2018-11-01 00:00:00

issn

2050-084X

pii

37550

journal_volume

7

pub_type

杂志文章

相关文献

eLife文献大全
  • Endangered wild salmon infected by newly discovered viruses.

    abstract::The collapse of iconic, keystone populations of sockeye (Oncorhynchus nerka) and Chinook (Oncorhynchus tshawytscha) salmon in the Northeast Pacific is of great concern. It is thought that infectious disease may contribute to declines, but little is known about viruses endemic to Pacific salmon. Metatranscriptomic sequ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.47615

    authors: Mordecai GJ,Miller KM,Di Cicco E,Schulze AD,Kaukinen KH,Ming TJ,Li S,Tabata A,Teffer A,Patterson DA,Ferguson HW,Suttle CA

    更新日期:2019-09-03 00:00:00

  • Adult axolotls can regenerate original neuronal diversity in response to brain injury.

    abstract::The axolotl can regenerate multiple organs, including the brain. It remains, however, unclear whether neuronal diversity, intricate tissue architecture, and axonal connectivity can be regenerated; yet, this is critical for recovery of function and a central aim of cell replacement strategies in the mammalian central n...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.13998

    authors: Amamoto R,Huerta VG,Takahashi E,Dai G,Grant AK,Fu Z,Arlotta P

    更新日期:2016-05-09 00:00:00

  • Ionic mechanisms underlying history-dependence of conduction delay in an unmyelinated axon.

    abstract::Axonal conduction velocity can change substantially during ongoing activity, thus modifying spike interval structures and, potentially, temporal coding. We used a biophysical model to unmask mechanisms underlying the history-dependence of conduction. The model replicates activity in the unmyelinated axon of the crusta...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.25382

    authors: Zhang Y,Bucher D,Nadim F

    更新日期:2017-07-10 00:00:00

  • Probe-Seq enables transcriptional profiling of specific cell types from heterogeneous tissue by RNA-based isolation.

    abstract::Recent transcriptional profiling technologies are uncovering previously-undefined cell populations and molecular markers at an unprecedented pace. While single cell RNA (scRNA) sequencing is an attractive approach for unbiased transcriptional profiling of all cell types, a complementary method to isolate and sequence ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.51452

    authors: Amamoto R,Garcia MD,West ER,Choi J,Lapan SW,Lane EA,Perrimon N,Cepko CL

    更新日期:2019-12-09 00:00:00

  • Bioengineered human myobundles mimic clinical responses of skeletal muscle to drugs.

    abstract::Existing in vitro models of human skeletal muscle cannot recapitulate the organization and function of native muscle, limiting their use in physiological and pharmacological studies. Here, we demonstrate engineering of electrically and chemically responsive, contractile human muscle tissues ('myobundles') using primar...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.04885

    authors: Madden L,Juhas M,Kraus WE,Truskey GA,Bursac N

    更新日期:2015-01-09 00:00:00

  • Mitochondrial volume fraction and translation duration impact mitochondrial mRNA localization and protein synthesis.

    abstract::Mitochondria are dynamic organelles that must precisely control their protein composition according to cellular energy demand. Although nuclear-encoded mRNAs can be localized to the mitochondrial surface, the importance of this localization is unclear. As yeast switch to respiratory metabolism, there is an increase in...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.57814

    authors: Tsuboi T,Viana MP,Xu F,Yu J,Chanchani R,Arceo XG,Tutucci E,Choi J,Chen YS,Singer RH,Rafelski SM,Zid BM

    更新日期:2020-08-07 00:00:00

  • Whole brain comparative anatomy using connectivity blueprints.

    abstract::Comparing the brains of related species faces the challenges of establishing homologies whilst accommodating evolutionary specializations. Here we propose a general framework for understanding similarities and differences between the brains of primates. The approach uses white matter blueprints of the whole cortex bas...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.35237

    authors: Mars RB,Sotiropoulos SN,Passingham RE,Sallet J,Verhagen L,Khrapitchev AA,Sibson N,Jbabdi S

    更新日期:2018-05-11 00:00:00

  • A molecular portrait of maternal sepsis from Byzantine Troy.

    abstract::Pregnancy complications are poorly represented in the archeological record, despite their importance in contemporary and ancient societies. While excavating a Byzantine cemetery in Troy, we discovered calcified abscesses among a woman's remains. Scanning electron microscopy of the tissue revealed 'ghost cells', result...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.20983

    authors: Devault AM,Mortimer TD,Kitchen A,Kiesewetter H,Enk JM,Golding GB,Southon J,Kuch M,Duggan AT,Aylward W,Gardner SN,Allen JE,King AM,Wright G,Kuroda M,Kato K,Briggs DE,Fornaciari G,Holmes EC,Poinar HN,Pepperell CS

    更新日期:2017-01-10 00:00:00

  • A receptor and neuron that activate a circuit limiting sucrose consumption.

    abstract::The neural control of sugar consumption is critical for normal metabolism. In contrast to sugar-sensing taste neurons that promote consumption, we identify a taste neuron that limits sucrose consumption in Drosophila. Silencing of the neuron increases sucrose feeding; optogenetic activation decreases it. The feeding i...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.24992

    authors: Joseph RM,Sun JS,Tam E,Carlson JR

    更新日期:2017-03-23 00:00:00

  • Listening in.

    abstract::Zebra finches communicate with each other in ways that are more complex than previously thought. ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.11665

    authors: Jarvis ED

    更新日期:2015-10-21 00:00:00

  • Multiple alleles at a single locus control seed dormancy in Swedish Arabidopsis.

    abstract::Seed dormancy is a complex life history trait that determines the timing of germination and is crucial for local adaptation. Genetic studies of dormancy are challenging, because the trait is highly plastic and strongly influenced by the maternal environment. Using a combination of statistical and experimental approach...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.22502

    authors: Kerdaffrec E,Filiault DL,Korte A,Sasaki E,Nizhynska V,Seren Ü,Nordborg M

    更新日期:2016-12-14 00:00:00

  • Tumor initiating cells induce Cxcr4-mediated infiltration of pro-tumoral macrophages into the brain.

    abstract::It is now clear that microglia and macrophages are present in brain tumors, but whether or how they affect initiation and development of tumors is not known. Exploiting the advantages of the zebrafish (Danio rerio) model, we showed that macrophages and microglia respond immediately upon oncogene activation in the brai...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.31918

    authors: Chia K,Mazzolini J,Mione M,Sieger D

    更新日期:2018-02-21 00:00:00

  • Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes.

    abstract::Extracellular vesicles (EVs) encompass a variety of vesicles secreted into the extracellular space. EVs have been implicated in promoting tumor metastasis, but the molecular composition of tumor-derived EV sub-types and the mechanisms by which molecules are sorted into EVs remain mostly unknown. We report the separati...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.47544

    authors: Temoche-Diaz MM,Shurtleff MJ,Nottingham RM,Yao J,Fadadu RP,Lambowitz AM,Schekman R

    更新日期:2019-08-22 00:00:00

  • Global landscape of phenazine biosynthesis and biodegradation reveals species-specific colonization patterns in agricultural soils and crop microbiomes.

    abstract::Phenazines are natural bacterial antibiotics that can protect crops from disease. However, for most crops it is unknown which producers and specific phenazines are ecologically relevant, and whether phenazine biodegradation can counter their effects. To better understand their ecology, we developed and environmentally...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.59726

    authors: Dar D,Thomashow LS,Weller DM,Newman DK

    更新日期:2020-09-15 00:00:00

  • Correlated magnetic resonance imaging and ultramicroscopy (MR-UM) is a tool kit to assess the dynamics of glioma angiogenesis.

    abstract::Neoangiogenesis is a pivotal therapeutic target in glioblastoma. Tumor monitoring requires imaging methods to assess treatment effects and disease progression. Until now mapping of the tumor vasculature has been difficult. We have developed a combined magnetic resonance and optical toolkit to study neoangiogenesis in ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.11712

    authors: Breckwoldt MO,Bode J,Kurz FT,Hoffmann A,Ochs K,Ott M,Deumelandt K,Krüwel T,Schwarz D,Fischer M,Helluy X,Milford D,Kirschbaum K,Solecki G,Chiblak S,Abdollahi A,Winkler F,Wick W,Platten M,Heiland S,Bendszus M,Tews

    更新日期:2016-02-02 00:00:00

  • Effects of microcompartmentation on flux distribution and metabolic pools in Chlamydomonas reinhardtii chloroplasts.

    abstract::Cells and organelles are not homogeneous but include microcompartments that alter the spatiotemporal characteristics of cellular processes. The effects of microcompartmentation on metabolic pathways are however difficult to study experimentally. The pyrenoid is a microcompartment that is essential for a carbon concent...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.37960

    authors: Küken A,Sommer F,Yaneva-Roder L,Mackinder LC,Höhne M,Geimer S,Jonikas MC,Schroda M,Stitt M,Nikoloski Z,Mettler-Altmann T

    更新日期:2018-10-11 00:00:00

  • Associations between sounds and actions in early auditory cortex of nonhuman primates.

    abstract::An individual may need to take different actions to the same stimulus in different situations to achieve a given goal. The selection of the appropriate action hinges on the previously learned associations between stimuli, actions, and outcomes in the situations. Here, using a go/no-go paradigm and a symmetrical reward...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.43281

    authors: Huang Y,Heil P,Brosch M

    更新日期:2019-04-04 00:00:00

  • Deep learning models predict regulatory variants in pancreatic islets and refine type 2 diabetes association signals.

    abstract::Genome-wide association analyses have uncovered multiple genomic regions associated with T2D, but identification of the causal variants at these remains a challenge. There is growing interest in the potential of deep learning models - which predict epigenome features from DNA sequence - to support inference concerning...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.51503

    authors: Wesolowska-Andersen A,Zhuo Yu G,Nylander V,Abaitua F,Thurner M,Torres JM,Mahajan A,Gloyn AL,McCarthy MI

    更新日期:2020-01-27 00:00:00

  • Slowly folding surface extension in the prototypic avian hepatitis B virus capsid governs stability.

    abstract::Hepatitis B virus (HBV) is an important but difficult to study human pathogen. Most basics of the hepadnaviral life-cycle were unraveled using duck HBV (DHBV) as a model although DHBV has a capsid protein (CP) comprising ~260 rather than ~180 amino acids. Here we present high-resolution structures of several DHBV caps...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.57277

    authors: Makbul C,Nassal M,Böttcher B

    更新日期:2020-08-14 00:00:00

  • Non-rhythmic head-direction cells in the parahippocampal region are not constrained by attractor network dynamics.

    abstract::Computational models postulate that head-direction (HD) cells are part of an attractor network integrating head turns. This network requires inputs from visual landmarks to anchor the HD signal to the external world. We investigated whether information about HD and visual landmarks is integrated in the medial entorhin...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.35949

    authors: Kornienko O,Latuske P,Bassler M,Kohler L,Allen K

    更新日期:2018-09-17 00:00:00

  • Transmission genetics of drug-resistant hepatitis C virus.

    abstract::Antiviral development is plagued by drug resistance and genetic barriers to resistance are needed. For HIV and hepatitis C virus (HCV), combination therapy has proved life-saving. The targets of direct-acting antivirals for HCV infection are NS3/4A protease, NS5A phosphoprotein and NS5B polymerase. Differential visual...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.32579

    authors: van Buuren N,Tellinghuisen TL,Richardson CD,Kirkegaard K

    更新日期:2018-03-28 00:00:00

  • A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain.

    abstract::Generating recombinant monoclonal antibodies (R-mAbs) from mAb-producing hybridomas offers numerous advantages that increase the effectiveness, reproducibility, and transparent reporting of research. We report here the generation of a novel resource in the form of a library of recombinant R-mAbs validated for neurosci...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.43322

    authors: Andrews NP,Boeckman JX,Manning CF,Nguyen JT,Bechtold H,Dumitras C,Gong B,Nguyen K,van der List D,Murray KD,Engebrecht J,Trimmer JS

    更新日期:2019-01-22 00:00:00

  • An ancient Pygo-dependent Wnt enhanceosome integrated by Chip/LDB-SSDP.

    abstract::TCF/LEF factors are ancient context-dependent enhancer-binding proteins that are activated by β-catenin following Wnt signaling. They control embryonic development and adult stem cell compartments, and their dysregulation often causes cancer. β-catenin-dependent transcription relies on the NPF motif of Pygo proteins. ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.09073

    authors: Fiedler M,Graeb M,Mieszczanek J,Rutherford TJ,Johnson CM,Bienz M

    更新日期:2015-08-27 00:00:00

  • Functional role of the type 1 pilus rod structure in mediating host-pathogen interactions.

    abstract::Uropathogenic E. coli (UPEC), which cause urinary tract infections (UTI), utilize type 1 pili, a chaperone usher pathway (CUP) pilus, to cause UTI and colonize the gut. The pilus rod, comprised of repeating FimA subunits, provides a structural scaffold for displaying the tip adhesin, FimH. We solved the 4.2 Å resoluti...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.31662

    authors: Spaulding CN,Schreiber HL 4th,Zheng W,Dodson KW,Hazen JE,Conover MS,Wang F,Svenmarker P,Luna-Rico A,Francetic O,Andersson M,Hultgren S,Egelman EH

    更新日期:2018-01-18 00:00:00

  • Depleting Trim28 in adult mice is well tolerated and reduces levels of α-synuclein and tau.

    abstract::Alzheimer's and Parkinson's disease are late onset neurodegenerative diseases that will require therapy over decades to mitigate the effects of disease-driving proteins such tau and α-synuclein (α-Syn). Previously we found that TRIM28 regulates the levels and toxicity of α-Syn and tau (journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.36768

    authors: Rousseaux MW,Revelli JP,Vázquez-Vélez GE,Kim JY,Craigen E,Gonzales K,Beckinghausen J,Zoghbi HY

    更新日期:2018-06-04 00:00:00

  • Increasing suppression of saccade-related transients along the human visual hierarchy.

    abstract::A key hallmark of visual perceptual awareness is robustness to instabilities arising from unnoticeable eye and eyelid movements. In previous human intracranial (iEEG) work (Golan et al., 2016) we found that excitatory broadband high-frequency activity transients, driven by eye blinks, are suppressed in higher-level bu...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.27819

    authors: Golan T,Davidesco I,Meshulam M,Groppe DM,Mégevand P,Yeagle EM,Goldfinger MS,Harel M,Melloni L,Schroeder CE,Deouell LY,Mehta AD,Malach R

    更新日期:2017-08-29 00:00:00

  • Fixation-pattern similarity analysis reveals adaptive changes in face-viewing strategies following aversive learning.

    abstract::Animals can effortlessly adapt their behavior by generalizing from past aversive experiences, allowing to avoid harm in novel situations. We studied how visual information was sampled by eye-movements during this process called fear generalization, using faces organized along a circular two-dimensional perceptual cont...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.44111

    authors: Kampermann L,Wilming N,Alink A,Büchel C,Onat S

    更新日期:2019-10-22 00:00:00

  • Acetylcholine is released in the basolateral amygdala in response to predictors of reward and enhances the learning of cue-reward contingency.

    abstract::The basolateral amygdala (BLA) is critical for associating initially neutral cues with appetitive and aversive stimuli and receives dense neuromodulatory acetylcholine (ACh) projections. We measured BLA ACh signaling and activity of neurons expressing CaMKIIα (a marker for glutamatergic principal cells) in mice during...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.57335

    authors: Crouse RB,Kim K,Batchelor HM,Girardi EM,Kamaletdinova R,Chan J,Rajebhosale P,Pittenger ST,Role LW,Talmage DA,Jing M,Li Y,Gao XB,Mineur YS,Picciotto MR

    更新日期:2020-09-18 00:00:00

  • Regulation of food intake by mechanosensory ion channels in enteric neurons.

    abstract::Regulation of food intake is fundamental to energy homeostasis in animals. The contribution of non-nutritive and metabolic signals in regulating feeding is unclear. Here we show that enteric neurons play a major role in regulating feeding through specialized mechanosensory ion channels in Drosophila. Modulating activi...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.04402

    authors: Olds WH,Xu T

    更新日期:2014-10-06 00:00:00

  • A transcriptomics resource reveals a transcriptional transition during ordered sarcomere morphogenesis in flight muscle.

    abstract::Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study sarcomerogenesis, we have generated a transcriptomics resource of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, most sarcomeric compon...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.34058

    authors: Spletter ML,Barz C,Yeroslaviz A,Zhang X,Lemke SB,Bonnard A,Brunner E,Cardone G,Basler K,Habermann BH,Schnorrer F

    更新日期:2018-05-30 00:00:00