Cyclooxygenase-2 inhibition reduces stress-induced affective pathology.

abstract：:Understanding the molecular mechanism of glaucoma and development of neuroprotectants is significantly hindered by the lack of a reliable animal model that accurately recapitulates human glaucoma. Here, we sought to develop a mouse model for the secondary glaucoma that is often observed in humans after silicone oil (S...

journal_title：eLife

authors： Zhang J,Li L,Huang H,Fang F,Webber HC,Zhuang P,Liu L,Dalal R,Tang PH,Mahajan VB,Sun Y,Li S,Zhang M,Goldberg JL,Hu Y

更新日期：2019-05-15 00:00:00

abstract：:Responses of sensory neurons are often modeled using a weighted combination of rectified linear subunits. Since these subunits often cannot be measured directly, a flexible method is needed to infer their properties from the responses of downstream neurons. We present a method for maximum likelihood estimation of subu...

journal_title：eLife

authors： Shah NP,Brackbill N,Rhoades C,Kling A,Goetz G,Litke AM,Sher A,Simoncelli EP,Chichilnisky EJ

更新日期：2020-03-09 00:00:00

abstract：:Lissencephaly ('smooth brain') is a severe brain disease associated with numerous symptoms, including cognitive impairment, and shortened lifespan. The main causative gene of this disease - lissencephaly-1 (LIS1) - has been a focus of intense scrutiny since its first identification almost 30 years ago. LIS1 is a criti...

journal_title：eLife

authors： Markus SM,Marzo MG,McKenney RJ

更新日期：2020-07-21 00:00:00

abstract：:Direct reprogramming of fibroblasts to neurons induces widespread cellular and transcriptional reconfiguration. Here, we characterized global epigenomic changes during the direct reprogramming of mouse fibroblasts to neurons using whole-genome base-resolution DNA methylation (mC) sequencing. We found that the pioneer ...

journal_title：eLife

authors： Luo C,Lee QY,Wapinski O,Castanon R,Nery JR,Mall M,Kareta MS,Cullen SM,Goodell MA,Chang HY,Wernig M,Ecker JR

更新日期：2019-01-15 00:00:00

abstract：:Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease involving cytotoxic conformations of Cu, Zn superoxide dismutase (SOD1). A major challenge in understanding ALS disease pathology has been the identification and atomic-level characterization of these conformers. Here, we use a combination ...

journal_title：eLife

authors： Sekhar A,Rumfeldt JA,Broom HR,Doyle CM,Bouvignies G,Meiering EM,Kay LE

更新日期：2015-06-23 00:00:00

abstract：: ...

journal_title：eLife

authors： MacKay CE,Leo MD,Fernández-Peña C,Hasan R,Yin W,Mata-Daboin A,Bulley S,Gammons J,Mancarella S,Jaggar JH

更新日期：2020-06-30 00:00:00

abstract：:R-loops are features of chromatin consisting of a strand of DNA hybridized to RNA, as well as the expelled complementary DNA strand. R-loops are enriched at promoters where they have recently been shown to have important roles in modifying gene expression. However, the location of promoter-associated R-loops and the g...

journal_title：eLife

authors： Dumelie JG,Jaffrey SR

更新日期：2017-10-26 00:00:00

abstract：:Ribonucleotide reductases (RNRs) convert ribonucleotides into deoxyribonucleotides, a reaction essential for DNA replication and repair. Human RNR requires two subunits for activity, the α subunit contains the active site, and the β subunit houses the radical cofactor. Here, we present a 3.3-Å resolution structure by ...

journal_title：eLife

authors： Brignole EJ,Tsai KL,Chittuluru J,Li H,Aye Y,Penczek PA,Stubbe J,Drennan CL,Asturias F

更新日期：2018-02-20 00:00:00

abstract：:Massed synchronised neuronal firing is detrimental to information processing. When networks of task-irrelevant neurons fire in unison, they mask the signal generated by task-critical neurons. On a macroscopic level, such synchronisation can contribute to alpha/beta (8-30 Hz) oscillations. Reducing the amplitude of the...

journal_title：eLife

authors： Griffiths BJ,Mayhew SD,Mullinger KJ,Jorge J,Charest I,Wimber M,Hanslmayr S

更新日期：2019-11-29 00:00:00

abstract：:Presynaptic homeostatic plasticity stabilizes information transfer at synaptic connections in organisms ranging from insect to human. By analogy with principles of engineering and control theory, the molecular implementation of PHP is thought to require postsynaptic signaling modules that encode homeostatic sensors, a...

journal_title：eLife

authors： Hauswirth AG,Ford KJ,Wang T,Fetter RD,Tong A,Davis GW

更新日期：2018-01-05 00:00:00

abstract：:Gene transcription can be activated by decreasing the duration of RNA polymerase II pausing in the promoter-proximal region, but how this is achieved remains unclear. Here we use a 'multi-omics' approach to demonstrate that the duration of polymerase pausing generally limits the productive frequency of transcription i...

journal_title：eLife

authors： Gressel S,Schwalb B,Decker TM,Qin W,Leonhardt H,Eick D,Cramer P

更新日期：2017-10-10 00:00:00

abstract：:Sleep oscillations in the neocortex and hippocampus are critical for the integration of new memories into stable generalized representations in neocortex. However, the role of the thalamus in this process is poorly understood. To determine the thalamic contribution to non-REM oscillations (sharp-wave ripples, SWRs; sl...

journal_title：eLife

authors： Varela C,Wilson MA

更新日期：2020-06-11 00:00:00

abstract：:The fitness effects of synonymous mutations, nucleotide changes that do not alter the encoded amino acid, have often been assumed to be neutral, but a growing body of evidence suggests otherwise. We used site-directed mutagenesis coupled with direct measures of competitive fitness to estimate the distribution of fitne...

journal_title：eLife

authors： Lebeuf-Taylor E,McCloskey N,Bailey SF,Hinz A,Kassen R

更新日期：2019-07-19 00:00:00

abstract：:Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher olfactory centre, the lateral horn (LH), is implicated in innate behaviour. However, our structural and functional understanding of the LH is scant, in large part due to a lack of sparse neurogenetic t...

journal_title：eLife

authors： Dolan MJ,Frechter S,Bates AS,Dan C,Huoviala P,Roberts RJ,Schlegel P,Dhawan S,Tabano R,Dionne H,Christoforou C,Close K,Sutcliffe B,Giuliani B,Li F,Costa M,Ihrke G,Meissner GW,Bock DD,Aso Y,Rubin GM,Jefferis GS

更新日期：2019-05-21 00:00:00

abstract：:Purkinje neurons are central to cerebellar function and show membrane bistability when recorded in vitro or in vivo under anesthesia. The existence of bistability in vivo in awake animals is disputed. Here, by recording intracellularly from Purkinje neurons in unanesthetized larval zebrafish (Danio rerio), we unequivo...

journal_title：eLife

authors： Sengupta M,Thirumalai V

更新日期：2015-09-29 00:00:00

abstract：:A small molecule called Sm4 can disrupt interactions involving a transcription factor called Sox18, while having little impact on other members of the SoxF family. ...

journal_title：eLife

authors： Kim I,Koh GY

更新日期：2017-01-31 00:00:00

abstract：:Strong kinetochore-microtubule attachments are essential for faithful segregation of sister chromatids during mitosis. The Dam1 and Ndc80 complexes are the main microtubule binding components of the Saccharomyces cerevisiae kinetochore. Cooperation between these two complexes enhances kinetochore-microtubule coupling ...

journal_title：eLife

authors： Kim JO,Zelter A,Umbreit NT,Bollozos A,Riffle M,Johnson R,MacCoss MJ,Asbury CL,Davis TN

更新日期：2017-02-13 00:00:00

abstract：:Episodic memories reflect a bound representation of multimodal features that can be reinstated with varying precision. Yet little is known about how brain networks involved in memory, including the hippocampus and posterior-medial (PM) and anterior-temporal (AT) systems, interact to support the quality and content of ...

journal_title：eLife

authors： Cooper RA,Ritchey M

更新日期：2019-03-22 00:00:00

abstract：:Mechanical force is a determinant of Notch signalling but the mechanism of force detection and its coupling to Notch are unclear. We propose a role for Piezo1 channels, which are mechanically-activated non-selective cation channels. In cultured microvascular endothelial cells, Piezo1 channel activation by either shear...

journal_title：eLife

authors： Caolo V,Debant M,Endesh N,Futers TS,Lichtenstein L,Bartoli F,Parsonage G,Jones EA,Beech DJ

更新日期：2020-06-02 00:00:00

abstract：:The central complex is a highly conserved insect brain region composed of morphologically stereotyped neurons that arborize in distinctively shaped substructures. The region is implicated in a wide range of behaviors and several modeling studies have explored its circuit computations. Most studies have relied on assum...

journal_title：eLife

authors： Franconville R,Beron C,Jayaraman V

更新日期：2018-08-20 00:00:00

abstract：:Cell growth and/or proliferation may require the reprogramming of metabolic pathways, whereby a switch from oxidative to glycolytic metabolism diverts glycolytic intermediates towards anabolic pathways. Herein, we identify a novel role for TRIM32 in the maintenance of glycolytic flux mediated by biochemical interactio...

journal_title：eLife

authors： Bawa S,Brooks DS,Neville KE,Tipping M,Sagar MA,Kollhoff JA,Chawla G,Geisbrecht BV,Tennessen JM,Eliceiri KW,Geisbrecht ER

更新日期：2020-03-30 00:00:00

abstract：:To elucidate new functions of sphingosine (Sph), we demonstrate that the spontaneous elevation of intracellular Sph levels via caged Sph leads to a significant and transient calcium release from acidic stores that is independent of sphingosine 1-phosphate, extracellular and ER calcium levels. This photo-induced Sph-dr...

journal_title：eLife

authors： Höglinger D,Haberkant P,Aguilera-Romero A,Riezman H,Porter FD,Platt FM,Galione A,Schultz C

更新日期：2015-11-27 00:00:00

abstract：:Hsp90 is a homodimeric ATP-dependent molecular chaperone that remodels its substrate 'client' proteins, facilitating their folding and activating them for biological function. Despite decades of research, the mechanism connecting ATP hydrolysis and chaperone function remains elusive. Particularly puzzling has been the...

journal_title：eLife

authors： Elnatan D,Betegon M,Liu Y,Ramelot T,Kennedy MA,Agard DA

更新日期：2017-07-25 00:00:00

abstract：:Secretory (S) Immunoglobulin (Ig) A is the predominant mucosal antibody, which binds pathogens and commensal microbes. SIgA is a polymeric antibody, typically containing two copies of IgA that assemble with one joining-chain (JC) to form dimeric (d) IgA that is bound by the polymeric Ig-receptor ectodomain, called sec...

journal_title：eLife

authors： Kumar Bharathkar S,Parker BW,Malyutin AG,Haloi N,Huey-Tubman KE,Tajkhorshid E,Stadtmueller BM

更新日期：2020-10-27 00:00:00

abstract：:The rhythm generating network for breathing must continuously adjust to changing metabolic and behavioral demands. Here, we examined network-based mechanisms in the mouse preBötzinger complex using substance P, a potent excitatory modulator of breathing frequency and stability, as a tool to dissect network properties ...

journal_title：eLife

authors： Baertsch NA,Ramirez JM

更新日期：2019-12-05 00:00:00

abstract：:The >800 human G protein-coupled receptors (GPCRs) are responsible for transducing diverse chemical stimuli to alter cell state- and are the largest class of drug targets. Their myriad structural conformations and various modes of signaling make it challenging to understand their structure and function. Here, we devel...

journal_title：eLife

authors： Jones EM,Lubock NB,Venkatakrishnan AJ,Wang J,Tseng AM,Paggi JM,Latorraca NR,Cancilla D,Satyadi M,Davis JE,Babu MM,Dror RO,Kosuri S

更新日期：2020-10-21 00:00:00

abstract：:Humans employ different strategies when making decisions. Previous research has reported reduced reliance on model-based strategies with aging, but it remains unclear whether this is due to cognitive or motivational factors. Moreover, it is not clear how aging affects the metacontrol of decision making, that is the dy...

journal_title：eLife

authors： Bolenz F,Kool W,Reiter AM,Eppinger B

更新日期：2019-08-09 00:00:00

abstract：:We determined whether the mutations found in ovarian cancers could be identified in the patients' ovarian cyst fluids. Tumor-specific mutations were detectable in the cyst fluids of 19 of 23 (83%) borderline tumors, 10 of 13 (77%) type I cancers, and 18 of 18 (100%) type II cancers. In contrast, no mutations were foun...

journal_title：eLife

authors： Wang Y,Sundfeldt K,Mateoiu C,Shih IeM,Kurman RJ,Schaefer J,Silliman N,Kinde I,Springer S,Foote M,Kristjansdottir B,James N,Kinzler KW,Papadopoulos N,Diaz LA,Vogelstein B

更新日期：2016-07-15 00:00:00

abstract：:Asymmetric disassembly of the synaptonemal complex (SC) is crucial for proper meiotic chromosome segregation. However, the signaling mechanisms that directly regulate this process are poorly understood. Here we show that the mammalian Rho GEF homolog, ECT-2, functions through the conserved RAS/ERK MAP kinase signaling...

journal_title：eLife

authors： Nadarajan S,Mohideen F,Tzur YB,Ferrandiz N,Crawley O,Montoya A,Faull P,Snijders AP,Cutillas PR,Jambhekar A,Blower MD,Martinez-Perez E,Harper JW,Colaiacovo MP

更新日期：2016-02-27 00:00:00

abstract：:The controversy surrounding 'gain-of-function' experiments on high-consequence avian influenza viruses has highlighted the role of ferret transmission experiments in studying the transmission potential of novel influenza strains. However, the mapping between influenza transmission in ferrets and in humans is unsubstan...

journal_title：eLife

authors： Buhnerkempe MG,Gostic K,Park M,Ahsan P,Belser JA,Lloyd-Smith JO

更新日期：2015-09-02 00:00:00