Building a functional connectome of the Drosophila central complex.

Abstract:

:The central complex is a highly conserved insect brain region composed of morphologically stereotyped neurons that arborize in distinctively shaped substructures. The region is implicated in a wide range of behaviors and several modeling studies have explored its circuit computations. Most studies have relied on assumptions about connectivity between neurons based on their overlap in light microscopy images. Here, we present an extensive functional connectome of Drosophila melanogaster's central complex at cell-type resolution. Using simultaneous optogenetic stimulation, calcium imaging and pharmacology, we tested the connectivity between 70 presynaptic-to-postsynaptic cell-type pairs. We identified numerous inputs to the central complex, but only a small number of output channels. Additionally, the connectivity of this highly recurrent circuit appears to be sparser than anticipated from light microscopy images. Finally, the connectivity matrix highlights the potentially critical role of a class of bottleneck interneurons. All data are provided for interactive exploration on a website.

journal_name

Elife

journal_title

eLife

authors

Franconville R,Beron C,Jayaraman V

doi

10.7554/eLife.37017

subject

Has Abstract

pub_date

2018-08-20 00:00:00

issn

2050-084X

pii

37017

journal_volume

7

pub_type

杂志文章

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