Yeast rises to the occasion.

abstract：:Tissue mechanics is important for development; however, the spatio-temporal dynamics of in vivo tissue stiffness is still poorly understood. We here developed tiv-AFM, combining time-lapse in vivo atomic force microscopy with upright fluorescence imaging of embryonic tissue, to show that during development local tissu...

journal_title：eLife

authors： Thompson AJ,Pillai EK,Dimov IB,Foster SK,Holt CE,Franze K

更新日期：2019-01-15 00:00:00

abstract：:Kinesin super family protein 2A (KIF2A), an ATP-dependent microtubule (MT) destabilizer, regulates cell migration, axon elongation, and pruning in the developing nervous system. KIF2A mutations have recently been identified in patients with malformed cortical development. However, postnatal KIF2A is continuously expre...

journal_title：eLife

authors： Homma N,Zhou R,Naseer MI,Chaudhary AG,Al-Qahtani MH,Hirokawa N

更新日期：2018-01-09 00:00:00

abstract：:Ribonucleotide reductases (RNRs) convert ribonucleotides into deoxyribonucleotides, a reaction essential for DNA replication and repair. Human RNR requires two subunits for activity, the α subunit contains the active site, and the β subunit houses the radical cofactor. Here, we present a 3.3-Å resolution structure by ...

journal_title：eLife

authors： Brignole EJ,Tsai KL,Chittuluru J,Li H,Aye Y,Penczek PA,Stubbe J,Drennan CL,Asturias F

更新日期：2018-02-20 00:00:00

abstract：:The medial subnucleus of the amygdala (MeA) plays a central role in processing sensory cues required for innate behaviors. However, whether there is a link between developmental programs and the emergence of inborn behaviors remains unknown. Our previous studies revealed that the telencephalic preoptic area (POA) embr...

journal_title：eLife

authors： Lischinsky JE,Sokolowski K,Li P,Esumi S,Kamal Y,Goodrich M,Oboti L,Hammond TR,Krishnamoorthy M,Feldman D,Huntsman M,Liu J,Corbin JG

更新日期：2017-03-13 00:00:00

abstract：:New ages for flowstone, sediments and fossil bones from the Dinaledi Chamber are presented. We combined optically stimulated luminescence dating of sediments with U-Th and palaeomagnetic analyses of flowstones to establish that all sediments containing Homo naledi fossils can be allocated to a single stratigraphic ent...

journal_title：eLife

authors： Dirks PH,Roberts EM,Hilbert-Wolf H,Kramers JD,Hawks J,Dosseto A,Duval M,Elliott M,Evans M,Grün R,Hellstrom J,Herries AI,Joannes-Boyau R,Makhubela TV,Placzek CJ,Robbins J,Spandler C,Wiersma J,Woodhead J,Berger LR

更新日期：2017-05-09 00:00:00

abstract：:Microtubules control different aspects of cell polarization. In cells with a radial microtubule system, a pivotal role in setting up asymmetry is attributed to the relative positioning of the centrosome and the nucleus. Here, we show that centrosome loss had no effect on the ability of endothelial cells to polarize an...

journal_title：eLife

authors： Martin M,Veloso A,Wu J,Katrukha EA,Akhmanova A

更新日期：2018-03-16 00:00:00

abstract：:Spore killers in fungi are selfish genetic elements that distort Mendelian segregation in their favor. It remains unclear how many species harbor them and how diverse their mechanisms are. Here, we discover two spore killers from a natural isolate of the fission yeast Schizosaccharomyces pombe. Both killers belong to ...

journal_title：eLife

authors： Hu W,Jiang ZD,Suo F,Zheng JX,He WZ,Du LL

更新日期：2017-06-20 00:00:00

abstract：:Toxoplasma gondii contains a limited subset of actin binding proteins. Here we show that the putative actin regulator cyclase-associated protein (CAP) is present in two different isoforms and its deletion leads to significant defects in some but not all actin dependent processes. We observe defects in cell-cell commun...

journal_title：eLife

authors： Hunt A,Russell MRG,Wagener J,Kent R,Carmeille R,Peddie CJ,Collinson L,Heaslip A,Ward GE,Treeck M

更新日期：2019-10-02 00:00:00

abstract：:Recent findings indicate a high level of specialization at the level of microcircuits and cell populations within brain structures with regards to the control of fear and anxiety. The hippocampus, however, has been treated as a unitary structure in anxiety and fear research despite mounting evidence that different hip...

journal_title：eLife

authors： Engin E,Smith KS,Gao Y,Nagy D,Foster RA,Tsvetkov E,Keist R,Crestani F,Fritschy JM,Bolshakov VY,Hajos M,Heldt SA,Rudolph U

更新日期：2016-03-14 00:00:00

abstract：:Retromer, including Vps35, Vps26, and Vps29, is a protein complex responsible for recycling proteins within the endolysosomal pathway. Although implicated in both Parkinson's and Alzheimer's disease, our understanding of retromer function in the adult brain remains limited, in part because Vps35 and Vps26 are essentia...

journal_title：eLife

authors： Ye H,Ojelade SA,Li-Kroeger D,Zuo Z,Wang L,Li Y,Gu JY,Tepass U,Rodal AA,Bellen HJ,Shulman JM

更新日期：2020-04-14 00:00:00

abstract：:Heterochromatin exerts a heritable form of eukaryotic gene repression and contributes to chromosome segregation fidelity and genome stability. However, to date there has been no quantitative evaluation of the stability of heterochromatic gene repression. We designed a genetic strategy to capture transient losses of ge...

journal_title：eLife

authors： Dodson AE,Rine J

更新日期：2015-01-12 00:00:00

abstract：:The production of action sequences is a fundamental aspect of motor skills. To examine whether primary motor cortex (M1) is involved in maintenance of sequential movements, we trained two monkeys (Cebus apella) to perform two sequential reaching tasks. In one task, sequential movements were instructed by visual cues, ...

journal_title：eLife

authors： Ohbayashi M

更新日期：2020-02-10 00:00:00

abstract：:Iron storage proteins are essential for cellular iron homeostasis and redox balance. Ferritin proteins are the major storage units for bioavailable forms of iron. Some organisms lack ferritins, and it is not known how they store iron. Encapsulins, a class of protein-based organelles, have recently been implicated in m...

journal_title：eLife

authors： Giessen TW,Orlando BJ,Verdegaal AA,Chambers MG,Gardener J,Bell DC,Birrane G,Liao M,Silver PA

更新日期：2019-07-08 00:00:00

abstract：:Here, we describe the case of a COVID-19 patient who developed recurring ventilator-associated pneumonia caused by Pseudomonas aeruginosa that acquired increasing levels of antimicrobial resistance (AMR) in response to treatment. Metagenomic analysis revealed the AMR genotype, while immunological analysis revealed mas...

journal_title：eLife

authors： Gregorova M,Morse D,Brignoli T,Steventon J,Hamilton F,Albur M,Arnold D,Thomas M,Halliday A,Baum H,Rice C,Avison MB,Davidson AD,Santopaolo M,Oliver E,Goenka A,Finn A,Wooldridge L,Amulic B,Boyton RJ,Altmann DM,But

更新日期：2020-12-17 00:00:00

abstract：:The mammalian auditory sensory epithelium has one of the most stereotyped cellular patterns known in vertebrates. Mechano-sensory hair cells are arranged in precise rows, with one row of inner and three rows of outer hair cells spanning the length of the spiral-shaped sensory epithelium. Aiding such precise cellular p...

journal_title：eLife

authors： Prajapati-DiNubila M,Benito-Gonzalez A,Golden EJ,Zhang S,Doetzlhofer A

更新日期：2019-06-12 00:00:00

abstract：:MicroRNAs (miRNAs) exert a broad influence over gene expression by directing effector activities that impinge on translation and stability of mRNAs. We recently discovered that the cap-binding protein 4EHP is a key component of the mammalian miRNA-Induced Silencing Complex (miRISC), which mediates gene silencing. Howe...

journal_title：eLife

authors： Jafarnejad SM,Chapat C,Matta-Camacho E,Gelbart IA,Hesketh GG,Arguello M,Garzia A,Kim SH,Attig J,Shapiro M,Morita M,Khoutorsky A,Alain T,Gkogkas CG,Stern-Ginossar N,Tuschl T,Gingras AC,Duchaine TF,Sonenberg N

更新日期：2018-02-07 00:00:00

abstract：:We present in vivo single-cell FRET measurements in the Escherichia coli chemotaxis system that reveal pervasive signaling variability, both across cells in isogenic populations and within individual cells over time. We quantify cell-to-cell variability of adaptation, ligand response, as well as steady-state output le...

journal_title：eLife

authors： Keegstra JM,Kamino K,Anquez F,Lazova MD,Emonet T,Shimizu TS

更新日期：2017-12-12 00:00:00

abstract：:Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of Saccharomyces cerevisiae 90S determined from three cryo-electron microscopy single particle...

journal_title：eLife

authors： Sun Q,Zhu X,Qi J,An W,Lan P,Tan D,Chen R,Wang B,Zheng S,Zhang C,Chen X,Zhang W,Chen J,Dong MQ,Ye K

更新日期：2017-02-28 00:00:00

abstract：:Patterns of spatial positioning of individuals within microbial communities are often critical to community function. However, understanding patterning in natural communities is hampered by the multitude of cell-cell and cell-environment interactions as well as environmental variability. Here, through simulations and ...

journal_title：eLife

authors： Momeni B,Brileya KA,Fields MW,Shou W

更新日期：2013-01-22 00:00:00

abstract：:Kinesin-1 and cytoplasmic dynein are microtubule (MT) motors that transport intracellular cargoes. It remains unclear how these motors move along MTs densely coated with obstacles of various sizes in the cytoplasm. Here, we tested the ability of single and multiple motors to bypass synthetic obstacles on MTs in vitro....

journal_title：eLife

authors： Ferro LS,Can S,Turner MA,ElShenawy MM,Yildiz A

更新日期：2019-09-09 00:00:00

abstract：:As part of the Reproducibility Project: Cancer Biology, we published a Registered Report (Blum et al., 2015), that described how we intended to replicate selected experiments from the paper 'Transcriptional amplification in tumor cells with elevated c-Myc' (Lin et al., 2012). Here we report the results. We found overe...

journal_title：eLife

authors： Lewis LM,Edwards MC,Meyers ZR,Talbot CC Jr,Hao H,Blum D,Reproducibility Project: Cancer Biology.

更新日期：2018-01-09 00:00:00

abstract：:The yeast Dsc E3 ligase complex has long been recognized as a Golgi-specific protein ubquitination system. It shares a striking sequence similarity to the Hrd1 complex that plays critical roles in the ER-associated degradation pathway. Using biochemical purification and mass spectrometry, we identified two novel Dsc s...

journal_title：eLife

authors： Yang X,Arines FM,Zhang W,Li M

更新日期：2018-01-22 00:00:00

abstract：:Aging is an intricate phenomenon associated with the gradual loss of physiological functions, and both nutrient sensing and proteostasis control lifespan. Although multiple approaches have facilitated the identification of candidate genes that govern longevity, the molecular mechanisms that link aging pathways are sti...

journal_title：eLife

authors： Chen YC,Jiang PH,Chen HM,Chen CH,Wang YT,Chen YJ,Yu CJ,Teng SC

更新日期：2018-12-05 00:00:00

abstract：:Brain connectivity maps display a delicate balance between individual variation and stereotypy, suggesting the existence of dedicated mechanisms that simultaneously permit and limit individual variation. We show that during the development of the Drosophila central nervous system, mutual inhibition among groups of nei...

journal_title：eLife

authors： Langen M,Koch M,Yan J,De Geest N,Erfurth ML,Pfeiffer BD,Schmucker D,Moreau Y,Hassan BA

更新日期：2013-03-05 00:00:00

abstract：:The precise pattern of motor neuron (MN) activation is essential for the execution of motor actions; however, the molecular mechanisms that give rise to specific patterns of MN activity are largely unknown. Phrenic MNs integrate multiple inputs to mediate inspiratory activity during breathing and are constrained to fi...

journal_title：eLife

authors： Vagnozzi AN,Garg K,Dewitz C,Moore MT,Cregg JM,Jeannotte L,Zampieri N,Landmesser LT,Philippidou P

更新日期：2020-01-16 00:00:00

abstract：:The progenitor cells of the developing liver can differentiate toward both hepatocyte and biliary cell fates. In addition to the established roles of TGFβ and Notch signaling in this fate specification process, there is increasing evidence that liver progenitors are sensitive to mechanical cues. Here, we utilized micr...

journal_title：eLife

authors： Kaylan KB,Berg IC,Biehl MJ,Brougham-Cook A,Jain I,Jamil SM,Sargeant LH,Cornell NJ,Raetzman LT,Underhill GH

更新日期：2018-12-27 00:00:00

abstract：:Bone strength is determined by its dense cortical shell, generated by unknown mechanisms. Here we use the Dmp1Cre:Socs3f/f mouse, with delayed cortical bone consolidation, to characterise cortical maturation and identify control signals. We show that cortical maturation requires a reduction in cortical porosity, and a...

journal_title：eLife

authors： Walker EC,Truong K,McGregor NE,Poulton IJ,Isojima T,Gooi JH,Martin TJ,Sims NA

更新日期：2020-05-27 00:00:00

abstract：:Hsp90 is a homodimeric ATP-dependent molecular chaperone that remodels its substrate 'client' proteins, facilitating their folding and activating them for biological function. Despite decades of research, the mechanism connecting ATP hydrolysis and chaperone function remains elusive. Particularly puzzling has been the...

journal_title：eLife

authors： Elnatan D,Betegon M,Liu Y,Ramelot T,Kennedy MA,Agard DA

更新日期：2017-07-25 00:00:00

abstract：:Group B Streptococcus (GBS) is the leading cause of invasive bacterial neonatal infections. Late-onset diseases (LOD) occur between 7 and 89 days of life and are largely due to the CC17 GBS hypervirulent clone. We studied the impact of estradiol (E2) and progesterone (P4), which impregnate the fetus during pregnancy, ...

journal_title：eLife

authors： Hays C,Touak G,Bouaboud A,Fouet A,Guignot J,Poyart C,Tazi A

更新日期：2019-11-11 00:00:00

abstract：:The Frank-Starling relation is a fundamental auto-regulatory property of the heart that ensures the volume of blood ejected in each heartbeat is matched to the extent of venous filling. At the cellular level, heart muscle cells generate higher force when stretched, but despite intense efforts the underlying molecular ...

journal_title：eLife

authors： Zhang X,Kampourakis T,Yan Z,Sevrieva I,Irving M,Sun YB

更新日期：2017-02-23 00:00:00