A novel L1CAM isoform with angiogenic activity generated by NOVA2-mediated alternative splicing.

abstract：:Kinesin-1 and cytoplasmic dynein are microtubule (MT) motors that transport intracellular cargoes. It remains unclear how these motors move along MTs densely coated with obstacles of various sizes in the cytoplasm. Here, we tested the ability of single and multiple motors to bypass synthetic obstacles on MTs in vitro....

journal_title：eLife

authors： Ferro LS,Can S,Turner MA,ElShenawy MM,Yildiz A

更新日期：2019-09-09 00:00:00

abstract：:Evolutionary changes in transcription networks are an important source of diversity across species, yet the quantitative consequences of network evolution have rarely been studied. Here we consider the transcriptional 'rewiring' of the three GAL genes that encode the enzymes needed for cells to convert galactose to gl...

journal_title：eLife

authors： Dalal CK,Zuleta IA,Mitchell KF,Andes DR,El-Samad H,Johnson AD

更新日期：2016-09-10 00:00:00

abstract：:Hepatitis B virus (HBV) is an important but difficult to study human pathogen. Most basics of the hepadnaviral life-cycle were unraveled using duck HBV (DHBV) as a model although DHBV has a capsid protein (CP) comprising ~260 rather than ~180 amino acids. Here we present high-resolution structures of several DHBV caps...

journal_title：eLife

authors： Makbul C,Nassal M,Böttcher B

更新日期：2020-08-14 00:00:00

abstract：:Proprioception, the sense of body position, movement, and associated forces, remains poorly understood, despite its critical role in movement. Most studies of area 2, a proprioceptive area of somatosensory cortex, have simply compared neurons' activities to the movement of the hand through space. Using motion tracking...

journal_title：eLife

authors： Chowdhury RH,Glaser JI,Miller LE

更新日期：2020-01-23 00:00:00

abstract：:The Sae2/CtIP protein is required for efficient processing of DNA double-strand breaks that initiate homologous recombination in eukaryotic cells. Sae2/CtIP is also important for survival of single-stranded Top1-induced lesions and CtIP is known to associate directly with transcription-associated complexes in mammalia...

journal_title：eLife

authors： Makharashvili N,Arora S,Yin Y,Fu Q,Wen X,Lee JH,Kao CH,Leung JW,Miller KM,Paull TT

更新日期：2018-12-07 00:00:00

abstract：:Ten countries have reported pfhrp2/pfhrp3 gene deletions since the first observation of pfhrp2-deleted parasites in 2012. In a previous study (Watson et al., 2017), we characterised the drivers selecting for pfhrp2/3 deletions and mapped the regions in Africa with the greatest selection pressure. In February 2018, the...

journal_title：eLife

authors： Watson OJ,Verity R,Ghani AC,Garske T,Cunningham J,Tshefu A,Mwandagalirwa MK,Meshnick SR,Parr JB,Slater HC

更新日期：2019-05-02 00:00:00

abstract：:Transient receptor potential (TRP) ion channels in peripheral sensory neurons are functionally regulated by hydrolysis of the phosphoinositide PI(4,5)P2 and changes in the level of protein kinase mediated phosphorylation following activation of various G protein coupled receptors. We now show that the activity of TRPM...

journal_title：eLife

authors： Quallo T,Alkhatib O,Gentry C,Andersson DA,Bevan S

更新日期：2017-08-15 00:00:00

abstract：:Glucose-6-phosphate dehydrogenase (G6PD) deficiency is believed to confer protection against Plasmodium falciparum malaria, but the precise nature of the protective effecthas proved difficult to define as G6PD deficiency has multiple allelic variants with different effects in males and females, and it has heterogeneou...

journal_title：eLife

authors： Clarke GM,Rockett K,Kivinen K,Hubbart C,Jeffreys AE,Rowlands K,Jallow M,Conway DJ,Bojang KA,Pinder M,Usen S,Sisay-Joof F,Sirugo G,Toure O,Thera MA,Konate S,Sissoko S,Niangaly A,Poudiougou B,Mangano VD,Bougouma EC

更新日期：2017-01-09 00:00:00

abstract：:Variation in the presentation of hereditary immunodeficiencies may be explained by genetic or environmental factors. Patients with mutations in HOIL1 (RBCK1) present with amylopectinosis-associated myopathy with or without hyper-inflammation and immunodeficiency. We report that barrier-raised HOIL-1-deficient mice exh...

journal_title：eLife

authors： MacDuff DA,Reese TA,Kimmey JM,Weiss LA,Song C,Zhang X,Kambal A,Duan E,Carrero JA,Boisson B,Laplantine E,Israel A,Picard C,Colonna M,Edelson BT,Sibley LD,Stallings CL,Casanova JL,Iwai K,Virgin HW

更新日期：2015-01-20 00:00:00

abstract：:In animal-based biomedical research, both the sex and the age of the animals studied affect disease phenotypes by modifying their susceptibility, presentation and response to treatment. The accurate reporting of experimental methods and materials, including the sex and age of animals, is essential so that other resear...

journal_title：eLife

authors： Flórez-Vargas O,Brass A,Karystianis G,Bramhall M,Stevens R,Cruickshank S,Nenadic G

更新日期：2016-03-03 00:00:00

abstract：:Rhythmic events recruit neuronal activity in the basal ganglia and cerebellum, but their roles remain elusive. In monkeys attempting to detect a single omission of isochronous visual stimulus, we found that neurons in the caudate nucleus showed increased activity for each stimulus in sequence, while those in the cereb...

journal_title：eLife

authors： Kameda M,Ohmae S,Tanaka M

更新日期：2019-09-06 00:00:00

abstract：:A strain of budding yeast that contains one large chromosome reveals how the telomere capping complex CST maintains linear but not circular chromosomes. ...

journal_title：eLife

authors： Nugent C,Sugimoto K

更新日期：2020-08-05 00:00:00

abstract：:Striatal dysfunction plays an important role in dystonia, but the striatal cell types that contribute to abnormal movements are poorly defined. We demonstrate that conditional deletion of the DYT1 dystonia protein torsinA in embryonic progenitors of forebrain cholinergic and GABAergic neurons causes dystonic-like twis...

journal_title：eLife

authors： Pappas SS,Darr K,Holley SM,Cepeda C,Mabrouk OS,Wong JM,LeWitt TM,Paudel R,Houlden H,Kennedy RT,Levine MS,Dauer WT

更新日期：2015-06-08 00:00:00

abstract：:Goal-directed behavior involves distributed neuronal circuits in the mammalian brain, including diverse regions of neocortex. However, the cellular basis of long-range cortico-cortical signaling during goal-directed behavior is poorly understood. Here, we recorded membrane potential of excitatory layer 2/3 pyramidal n...

journal_title：eLife

authors： Yamashita T,Petersen CCh

更新日期：2016-06-21 00:00:00

abstract：:The new open-access journal eLife has launched, making its first content available in PubMed Central. In addition to publishing science of the highest quality, the journal aims to improve both the peer-review process and the presentation of new research results. ...

journal_title：eLife

authors： Schekman R,Patterson M,Watt F,Weigel D

更新日期：2012-10-15 00:00:00

abstract：:Cell surface reception of Sonic hedgehog (Shh) must ensure that the graded morphogenic signal is interpreted accordingly in neighboring cells to specify tissue patterns during development. Here, we report endocytic sorting signals for the receptor Patched1 (Ptch1), comprising two 'PPXY' motifs, that direct it to degra...

journal_title：eLife

authors： Yue S,Tang LY,Tang Y,Tang Y,Shen QH,Ding J,Chen Y,Zhang Z,Yu TT,Zhang YE,Cheng SY

更新日期：2014-06-12 00:00:00

abstract：:Baboons, members of the genus Papio, comprise six closely related species distributed throughout sub-Saharan Africa and southwest Arabia. The species exhibit more ecological flexibility and a wider range of social systems than many other primates. This article summarizes our current knowledge of the natural history of...

journal_title：eLife

authors： Fischer J,Higham JP,Alberts SC,Barrett L,Beehner JC,Bergman TJ,Carter AJ,Collins A,Elton S,Fagot J,Ferreira da Silva MJ,Hammerschmidt K,Henzi P,Jolly CJ,Knauf S,Kopp GH,Rogers J,Roos C,Ross C,Seyfarth RM,Silk J,

更新日期：2019-11-12 00:00:00

abstract：:To move the body, the brain must precisely coordinate patterns of activity among diverse populations of motor neurons. Here, we use in vivo calcium imaging, electrophysiology, and behavior to understand how genetically-identified motor neurons control flexion of the fruit fly tibia. We find that leg motor neurons exhi...

journal_title：eLife

authors： Azevedo AW,Dickinson ES,Gurung P,Venkatasubramanian L,Mann RS,Tuthill JC

更新日期：2020-06-03 00:00:00

abstract：:Responses of sensory neurons represent stimulus information, but are also influenced by internal state. For example, when monkeys direct their attention to a visual stimulus, the response gain of specific subsets of neurons in visual cortex changes. Here, we develop a functional model of population activity to investi...

journal_title：eLife

authors： Rabinowitz NC,Goris RL,Cohen M,Simoncelli EP

更新日期：2015-11-02 00:00:00

abstract：:hIAPP fibrils are associated with Type-II Diabetes, but the link of hIAPP structure to islet cell death remains elusive. Here we observe that hIAPP fibrils are cytotoxic to cultured pancreatic β-cells, leading us to determine the structure and cytotoxicity of protein segments composing the amyloid spine of hIAPP. Usin...

journal_title：eLife

authors： Krotee P,Rodriguez JA,Sawaya MR,Cascio D,Reyes FE,Shi D,Hattne J,Nannenga BL,Oskarsson ME,Philipp S,Griner S,Jiang L,Glabe CG,Westermark GT,Gonen T,Eisenberg DS

更新日期：2017-01-03 00:00:00

abstract：:The TP53 tumor-suppressor gene is mutated in >50% of human tumors and Li-Fraumeni patients with germ line inactivation are predisposed to developing cancer. Here, we generated tp53 deleted zebrafish that spontaneously develop malignant peripheral nerve-sheath tumors, angiosarcomas, germ cell tumors, and an aggressive ...

journal_title：eLife

authors： Ignatius MS,Hayes MN,Moore FE,Tang Q,Garcia SP,Blackburn PR,Baxi K,Wang L,Jin A,Ramakrishnan A,Reeder S,Chen Y,Nielsen GP,Chen EY,Hasserjian RP,Tirode F,Ekker SC,Langenau DM

更新日期：2018-09-07 00:00:00

abstract：:Generating recombinant monoclonal antibodies (R-mAbs) from mAb-producing hybridomas offers numerous advantages that increase the effectiveness, reproducibility, and transparent reporting of research. We report here the generation of a novel resource in the form of a library of recombinant R-mAbs validated for neurosci...

journal_title：eLife

authors： Andrews NP,Boeckman JX,Manning CF,Nguyen JT,Bechtold H,Dumitras C,Gong B,Nguyen K,van der List D,Murray KD,Engebrecht J,Trimmer JS

更新日期：2019-01-22 00:00:00

abstract：:It is widely argued that personalized instruction based on individual differences in learning styles or genetic predispositions could improve learning outcomes. However, this proposition has resisted clear demonstration in human studies, where it is difficult to control experience and quantify outcomes. Here, we take ...

journal_title：eLife

authors： Mets DG,Brainard MS

更新日期：2019-09-17 00:00:00

abstract：:Recurrent somatic mutations of H3F3A in aggressive pediatric high-grade gliomas generate K27M or G34R/V mutant histone H3.3. H3.3-G34R/V mutants are common in tumors with mutations in p53 and ATRX, an H3.3-specific chromatin remodeler. To gain insight into the role of H3-G34R, we generated fission yeast that express o...

journal_title：eLife

authors： Yadav RK,Jablonowski CM,Fernandez AG,Lowe BR,Henry RA,Finkelstein D,Barnum KJ,Pidoux AL,Kuo YM,Huang J,O'Connell MJ,Andrews AJ,Onar-Thomas A,Allshire RC,Partridge JF

更新日期：2017-07-18 00:00:00

abstract：:The incubation period for typhoid, polio, measles, leukemia and many other diseases follows a right-skewed, approximately lognormal distribution. Although this pattern was discovered more than sixty years ago, it remains an open question to explain its ubiquity. Here, we propose an explanation based on evolutionary dy...

journal_title：eLife

authors： Ottino-Loffler B,Scott JG,Strogatz SH

更新日期：2017-12-21 00:00:00

abstract：:Synaptic positions underlie precise circuit connectivity. Synaptic positions can be established during embryogenesis and sustained during growth. The mechanisms that sustain synaptic specificity during allometric growth are largely unknown. We performed forward genetic screens in C. elegans for regulators of this proc...

journal_title：eLife

authors： Fan J,Ji T,Wang K,Huang J,Wang M,Manning L,Dong X,Shi Y,Zhang X,Shao Z,Colón-Ramos DA

更新日期：2020-04-07 00:00:00

abstract：:The mesiotemporal lobe (MTL) is implicated in many cognitive processes, is compromised in numerous brain disorders, and exhibits a gradual cytoarchitectural transition from six-layered parahippocampal isocortex to three-layered hippocampal allocortex. Leveraging an ultra-high-resolution histological reconstruction of ...

journal_title：eLife

authors： Paquola C,Benkarim O,DeKraker J,Larivière S,Frässle S,Royer J,Tavakol S,Valk S,Bernasconi A,Bernasconi N,Khan A,Evans AC,Razi A,Smallwood J,Bernhardt BC

更新日期：2020-11-04 00:00:00

abstract：:The basolateral amygdala (BLA) is critical for associating initially neutral cues with appetitive and aversive stimuli and receives dense neuromodulatory acetylcholine (ACh) projections. We measured BLA ACh signaling and activity of neurons expressing CaMKIIα (a marker for glutamatergic principal cells) in mice during...

journal_title：eLife

authors： Crouse RB,Kim K,Batchelor HM,Girardi EM,Kamaletdinova R,Chan J,Rajebhosale P,Pittenger ST,Role LW,Talmage DA,Jing M,Li Y,Gao XB,Mineur YS,Picciotto MR

更新日期：2020-09-18 00:00:00

abstract：:SUMO-modification of nuclear proteins has profound effects on gene expression. However, non-toxic chemical tools that modulate sumoylation in cells are lacking. Here, to identify small molecule sumoylation inhibitors we developed a cell-based screen that focused on the well-sumoylated substrate, human Liver Receptor H...

journal_title：eLife

authors： Suzawa M,Miranda DA,Ramos KA,Ang KK,Faivre EJ,Wilson CG,Caboni L,Arkin MR,Kim YS,Fletterick RJ,Diaz A,Schneekloth JS,Ingraham HA

更新日期：2015-12-11 00:00:00

abstract：:Recent epidemiological and laboratory-based studies suggest that the anti-diabetic drug metformin prevents cancer progression. How metformin diminishes tumor growth is not fully understood. In this study, we report that in human cancer cells, metformin inhibits mitochondrial complex I (NADH dehydrogenase) activity and...

journal_title：eLife

authors： Wheaton WW,Weinberg SE,Hamanaka RB,Soberanes S,Sullivan LB,Anso E,Glasauer A,Dufour E,Mutlu GM,Budigner GS,Chandel NS

更新日期：2014-05-13 00:00:00