abstract：:Silent information regulator type-1 (SIRT1) is crucial during the development of acute respiratory distress syndrome (ARDS). We aimed to explore whether SIRT1 activation could protect against ARDS. SIRT1 was activated by its agonist SRT1720. ARDS was induced by intraperitoneal injection of 5 mg/kg lipopolysaccharide (...

journal_title：Journal of biochemistry

authors： Zhan Y,Yang C,Zhang Q,Yao L

更新日期：2021-01-22 00:00:00

abstract：:We investigated the expression and functions of circular RNA (circRNA) circNINL and miR-921 in breast cancer (BC) in this study. We found that the expression of circNINL increased while the expression of miR-921 decreased in BC tissues and cell lines, and their anomalous expressions were associated with malignant feat...

journal_title：Journal of biochemistry

authors： Xu C,Yu H,Yin X,Zhang J,Liu C,Qi H,Liu P

更新日期：2021-01-21 00:00:00

abstract：:Tardigrades, a phylum of meiofaunal organisms, exhibit extraordinary tolerance to various environmental conditions, including extreme temperatures (-272 to 151 °C) and exposure to ionizing radiation. Proteins from anhydrobiotic tardigrades with homology to known proteins from other organisms are new potential targets ...

journal_title：Journal of biochemistry

authors： Kobayashi K,Kim J,Fukuda Y,Kozawa T,Inoue T

更新日期：2021-01-21 00:00:00

abstract：:Stem cells divide and undergo self-renewal depending on the signals received from the stem cell niche. This phenomenon is indispensable to maintain tissues and organs in individuals. However, not all the molecular factors and mechanisms of self-renewal are known. In our previous study, we reported that glycosylphospha...

journal_title：Journal of biochemistry

authors： Rikitake M,Matsuda A,Murata D,Dejima K,Nomura KH,Abbott KL,Mitani S,Nomura K

更新日期：2020-12-26 00:00:00

abstract：:Long non-coding RNA is an endogenous non-coding RNA that has currently been proved to be an important player in cancer cell biology. In the present study, we investigated the biological role of PHACTR2-AS1 in tongue squamous cell carcinoma (TSCC). PHACTR2-AS1 was preferentially localized in the cytoplasm, and was nota...

journal_title：Journal of biochemistry

authors： Yuan F,Miao Z,Chen W,Wu F,Wei C,Yong J,Xiao C

更新日期：2020-12-26 00:00:00

abstract：:Extracellular vesicles (EVs) are small particles that are naturally released from various types of cells. EVs contain a wide variety of cellular components, such as proteins, nucleic acids, lipids and metabolites, which facilitate intercellular communication in diverse biological processes. In the tumor microenvironme...

journal_title：Journal of biochemistry

authors： Yamauchi T,Moroishi T

更新日期：2020-11-23 00:00:00

abstract：:In the nucleus of eukaryotic cells, chromatin is tethered to the nuclear envelope (NE), wherein inner nuclear membrane proteins (INMPs) play major roles. However, in Xenopus blastula, chromatin tethering to the NE depends on nuclear filamentous actin that develops in a blastula-specific manner. To investigate whether ...

journal_title：Journal of biochemistry

authors： Oda H,Kato S,Ohsumi K,Iwabuchi M

更新日期：2020-11-10 00:00:00

abstract：:Deleting the gene for small RNA GcvB in Esherichia coli was found to increase the sensitivity to several aminoglycoside antibiotics, such as neomycin, streptomycin, kanamycin, kasugamycin and spectinomycin, at low concentrations. GcvB, conserved in gram-negative enteric bacteria, is known to negatively control the exp...

journal_title：Journal of biochemistry

authors： Muto A,Goto S,Kurita D,Ushida C,Himeno H

更新日期：2020-11-10 00:00:00

abstract：:Gastric cancer (GC) is one of the most common cancers in gastrointestinal malignant tumours. Long non-coding RNAs were widely reported to play a significant role in the regulation of occurrence or development of tumours. Bioinformatics analysis and a wide range of experiments were conducted to explore the expression s...

journal_title：Journal of biochemistry

authors： Xin H,Yan Z,Cao J

更新日期：2020-11-01 00:00:00

abstract：:In eukaryotes, ribonuclease H1 (RNase H1) is involved in the processing and removal of RNA/DNA hybrids in both nuclear and mitochondrial DNA. The enzyme comprises a C-terminal catalytic domain and an N-terminal hybrid-binding domain (HBD), separated by a linker of variable length, 115 amino acids in Drosophila melanog...

journal_title：Journal of biochemistry

authors： González de Cózar JM,Carretero-Junquera M,Ciesielski GL,Miettinen SM,Varjosalo M,Kaguni LS,Dufour E,Jacobs HT

更新日期：2020-11-01 00:00:00

abstract：:ZFP36L1 is an RNA-binding protein responsible for mRNA decay in the cytoplasm. ZFP36L1 has also been suggested as a nuclear-cytoplasmic shuttling protein because it contains a potential nuclear localization signal and a nuclear export signal. However, it remains unclear how the nuclear localization of ZFP36L1 is contr...

journal_title：Journal of biochemistry

authors： Matsuura Y,Noguchi A,Sakai S,Yokota N,Kawahara H

更新日期：2020-11-01 00:00:00

abstract：:RING finger protein 8 (RNF8) is an E3 ligase that is pivotal for DNA repair. However, the role of RNF8 in ulcerative colitis (UC) remains unclear. The aim of this study is to investigate the effect and the mechanism of RNF8 on UC model induced by trinitrobenzene sulfonic acid (TNBS) in mice. Lentiviruses overexpressin...

journal_title：Journal of biochemistry

authors： Zhu Y,Shi Y,Ke X,Xuan L,Ma Z

更新日期：2020-11-01 00:00:00

abstract：:Glycoengineering of therapeutic proteins has been applied to improve the clinical efficacy of several therapeutics. Here, we examined the effect of glycosylation on the properties of the Fab of the therapeutic antibody, adalimumab. An N-glycosylation site was introduced at position 178 of the H-chain constant region o...

journal_title：Journal of biochemistry

authors： Nakamura H,Kiyoshi M,Anraku M,Hashii N,Oda-Ueda N,Ueda T,Ohkuri T

更新日期：2020-10-27 00:00:00

abstract：:Urate oxidases (UOs) catalyze the cofactor-independent oxidation of uric acid, and an extensive water network in the active site has been suggested to play an essential role in the catalysis. For our present analysis of the structure and function of the water network, the crystal qualities of Bacillus sp. TB-90 urate ...

journal_title：Journal of biochemistry

authors： Hibi T,Itoh T

更新日期：2020-10-01 00:00:00

abstract：:The crystal structure of an adenylate kinase from an extremophilic archaeon Aeropyrum pernix was determined in complex with full ligands, ATP-Mg2+ and AMP, at a resolution of 2.0 Å. The protein forms a trimer as found for other adenylate kinases from archaea. Interestingly, the reacting three atoms, two phosphorus and...

journal_title：Journal of biochemistry

authors： Shibanuma Y,Nemoto N,Yamamoto N,Sampei GI,Kawai G

更新日期：2020-09-01 00:00:00

abstract：:Actin-microtubule crosstalk is implicated in the formation of cellular protrusions, but the mechanism remains unclear. In this study, we examined the regulation of cell protrusion involving a ubiquitously expressed microtubule-associated protein (MAP) 4, and its superfamily proteins, neuronal MAP2 and tau. Fluorescenc...

journal_title：Journal of biochemistry

authors： Doki C,Nishida K,Saito S,Shiga M,Ogara H,Kuramoto A,Kuragano M,Nozumi M,Igarashi M,Nakagawa H,Kotani S,Tokuraku K

更新日期：2020-09-01 00:00:00

abstract：:Aberrant DNA methylation is a common form of epigenetic alterations and it has been proved to be closely related to many cancers, while its role in epidermal growth factor receptor (EGFR)-mutated non-small cell lung cancer (NSCLC) is not clear. This study focuses on the role of DNA methyltransferase 1 (DNMT1) in EGFR-...

journal_title：Journal of biochemistry

authors： Wu XY,Chen HC,Li WW,Yan JD,Lv RY

更新日期：2020-08-01 00:00:00

abstract：:Antizyme (AZ) interacts with ornithine decarboxylase, which catalyzes the first step of polyamine biosynthesis and recruits it to the proteasome for degradation. Synthesizing the functional AZ protein requires transition of the reading frame at the termination codon. This programmed +1 ribosomal frameshifting is induc...

journal_title：Journal of biochemistry

authors： Oguro A,Shigeta T,Machida K,Suzuki T,Iwamoto T,Matsufuji S,Imataka H

更新日期：2020-08-01 00:00:00

abstract：:The readthrough of premature termination codon (PTC) by ribosome sometimes produces full-length proteins. We previously reported a readthrough of PTC of glycosyltransferase gene B4GALNT1 with hereditary spastic paraplegia (HSP). Here we featured the readthrough of B4GALNT1 of two mutants, M4 and M2 with PTC by immunob...

journal_title：Journal of biochemistry

authors： Yesmin F,Bhuiyan RH,Ohmi Y,Ohkawa Y,Tajima O,Okajima T,Furukawa K,Furukawa K

更新日期：2020-08-01 00:00:00

abstract：:We have recently developed an in vitro yeast reconstituted translation system, which is capable of synthesizing long polypeptides. Utilizing the system, we examined the role of eIF5A and its hypusine modification in translating polyproline sequence within long open reading frames. We found that polyproline motif inser...

journal_title：Journal of biochemistry

authors： Abe T,Nagai R,Shimazaki S,Kondo S,Nishimura S,Sakaguchi Y,Suzuki T,Imataka H,Tomita K,Takeuchi-Tomita N

更新日期：2020-05-01 00:00:00

abstract：:Dynamic functionality of mitochondria is maintained by continual fusion and fission events. A mitochondrial outer membrane protein Fzo1 plays a pivotal role upon mitochondrial fusion by homo-oligomerization to tether fusing mitochondria. Fzo1 is tightly regulated by ubiquitylations and the ubiquitin-responsible AAA pr...

journal_title：Journal of biochemistry

authors： Nahar S,Chowdhury A,Ogura T,Esaki M

更新日期：2020-03-01 00:00:00

abstract：:The mitochondrion is an essential organelle for a wide range of cellular processes, including energy production, metabolism, signal transduction and cell death. To execute these functions, mitochondria regulate their size, number, morphology and distribution in cells via mitochondrial division and fusion. In addition,...

journal_title：Journal of biochemistry

authors： Murata D,Arai K,Iijima M,Sesaki H

更新日期：2020-03-01 00:00:00

abstract：:Mitochondria play many critical roles in cells, not only by supplying energy, but also by supplying metabolites, buffering Ca2+ levels and regulating apoptosis. During oocyte maturation and subsequent embryo development, mitochondria change their morphology by membrane fusion and fission, and coordinately undergo mult...

journal_title：Journal of biochemistry

authors： Udagawa O,Ishihara N

更新日期：2020-03-01 00:00:00

abstract：:This study aims to study the effects of intra-nuclear lncRNA MEG3 on the progression of prostate cancer and the underlying mechanisms. Expressions of relative molecules were detected by Quantitative real time PCR (qRT-PCR) and western blot. Chromatin immunoprecipitation and RNA immunoprecipitation (RIP) assays were us...

journal_title：Journal of biochemistry

authors： Zhou Y,Yang H,Xia W,Cui L,Xu R,Lu H,Xue D,Tian Z,Ding T,Cao Y,Shi Q,He X

更新日期：2020-03-01 00:00:00

abstract：:Protein kinases are essential mediators of cellular signal transduction and are often dysregulated in disease. Among these, protein tyrosine kinases (PTKs) have received specific interest due to their common roles in various diseases including cancer, and emerging observations indicating that PTK signalling pathways a...

journal_title：Journal of biochemistry

authors： Dustin CM,Heppner DE,Lin MJ,van der Vliet A

更新日期：2020-02-01 00:00:00

abstract：:MicroRNAs (miRNAs) serve as key regulators in human disorders. Previous research reported that miR-211-5p is down-regulated in osteoarthritis (OA) and that Fibulin-4 inhibits chondrocyte differentiation. However, the role of miR-211-5p in the development of OA has not been clarified, and its downstream target has not ...

journal_title：Journal of biochemistry

authors： Liu H,Luo J

更新日期：2019-12-01 00:00:00

abstract：:MscL is a mechanosensitive channel that undergoes a global conformational change upon application of membrane stretching. To elucidate how the structural stability and flexibility occur, we isolated temperature-sensitive (Ts) mutants of Escherichia coli MscL that allowed cell growth at 32°C but not at 42°C. Two Ts mut...

journal_title：Journal of biochemistry

authors： Owada N,Yoshida M,Morita K,Yoshimura K

更新日期：2019-09-01 00:00:00

abstract：:The ferric uptake regulator A (FurA) plays an essential role in responding to oxidative stress in mycobacteria. The genome of Mycobacterium smegmatis harbours three FurA orthologs; however, the potential cross-talk and contribution to drug resistance of different furA operon remain underdetermined. In this study, we c...

journal_title：Journal of biochemistry

authors： Gao CH,Wei WP,Tao HL,Cai LK,Jia WZ,Hu L,Yang M

更新日期：2019-09-01 00:00:00

abstract：:The E2F transcription factors (TFs), which control the progression of the cell cycle in response to DNA-damage and various stresses, are known to interact with a tumor suppressor, Retinoblastoma 1 (RB1). We previously showed that the response of the human RB1 promoter to a 12-O-tetradecanoylphorbol-13-acetate (TPA) in...

journal_title：Journal of biochemistry

authors： Hamada H,Goto Y,Arakawa J,Murayama E,Ogawa Y,Konno M,Oyama T,Asai M,Sato A,Tanuma SI,Uchiumi F

更新日期：2019-06-14 00:00:00

abstract：:Neuronal migration is a crucial event in neuronal development for the construction of brain architecture and neuronal networks. Newborn neurons proliferate in the germinal zone and start migration toward their final destination. Migrating neurons adopt different routes, cell shapes and migratory modes depending on ext...

journal_title：Journal of biochemistry

authors： Horigane SI,Ozawa Y,Yamada H,Takemoto-Kimura S

更新日期：2019-05-01 00:00:00

abstract：:This study presents the structural, kinetic and thermodynamic characterizations of previously unknown submicellar concentrations of SDS-induced molten globule (MGSDS) state of a highly negatively charged base-denatured ferricytochrome c (UB-state) at pH ∼12.8 (±0.2). The far-UV CD, near-UV CD, ANS-fluorescence data of...

journal_title：Journal of biochemistry

authors： Jain R,Sharma D,Kumar R,Kumar R

更新日期：2019-02-01 00:00:00

abstract：:A newly isolated binding protein of peroxisomal targeting signal type 2 (PTS2) receptor Pex7, termed P7BP2, is transported into peroxisomes by binding to the longer isoform of Pex5p, Pex5pL, via Pex7p. The binding to Pex7p and peroxisomal localization of P7BP2 depends on the cleavable PTS2 in the N-terminal region, su...

journal_title：Journal of biochemistry

authors： Niwa H,Miyauchi-Nanri Y,Okumoto K,Mukai S,Noi K,Ogura T,Fujiki Y

更新日期：2018-12-01 00:00:00

abstract：:A small nuclear protein, C1D, has roles in various cellular processes, transcription regulation, genome stability surveillance, DNA repair and RNA processing, all of which are required to maintain the host life cycles. In the previous report, C1D directly interacts with XPB, a component of the nucleotide excision repa...

journal_title：Journal of biochemistry

authors： Tomita T,Ieguchi K,Takita M,Tsukahara F,Yamada M,Egly JM,Maru Y

更新日期：2018-12-01 00:00:00

abstract：:Epithelial-mesenchymal transition (EMT) is a morphogenetic process of cells that adopt an epithelial organization in their developmental ontogeny or homeostatic maintenance. Abnormalities in EMT regulation result in many malignant tumours in the human body. Tumours associated with the haematopoietic system, however, a...

journal_title：Journal of biochemistry

authors： Hamidi S,Sheng G

更新日期：2018-10-01 00:00:00

abstract：:Mitotic kinesin Eg5 plays an important physiological role in cell division. Several small-molecule inhibitors of Eg5 are the focus of cancer therapies. Azobenzene is a photochromic compound exhibiting cis-trans isomerization upon ultraviolet (UV) and visible (VIS) light irradiation. Photochromic compounds of azobenzen...

journal_title：Journal of biochemistry

authors： Sadakane K,Alrazi IMD,Maruta S

更新日期：2018-10-01 00:00:00

abstract：:Streptococcus pyogenes nuclease A (SpnA) and streptococcal 5' nucleosidase A (S5nA) are two recently described virulence factors from the human pathogen S. pyogenes. In vitro studies have shown that SpnA is a nuclease that cleaves ssDNA and dsDNA, including the DNA backbone of neutrophil extracellular traps. S5nA was ...

journal_title：Journal of biochemistry

authors： Soh KY,Loh JMS,Proft T

更新日期：2018-08-01 00:00:00

abstract：:Heat shock protein 70 (Hsp70) chaperone systems consist of Hsp70, Hsp40 and a nucleotide-exchange factor and function to help unfolded proteins achieve their native conformations. Typical Hsp40s assume a homodimeric structure and have both chaperone and cochaperone activity. The dimeric structure is critical for chape...

journal_title：Journal of biochemistry

authors： Uchida T,Kanemori M

更新日期：2018-08-01 00:00:00

abstract：:Chondroitin sulphate proteoglycan 4 (CSPG4) is a cell surface proteoglycan highly expressed by tumour, perivascular and oligodendrocyte cells and known to be involved cell adhesion and migration. This study showed that CSPG4 was present as a proteoglycan on the cell surface of two melanoma cell lines, MM200 and Me1007...

journal_title：Journal of biochemistry

authors： Tang F,Lord MS,Stallcup WB,Whitelock JM

更新日期：2018-05-01 00:00:00

abstract：:Skeletal muscle is composed of multinuclear cells called myofibers. Muscular dystrophy (a genetic muscle disorder) induces instability in the cell membrane of myofibers and eventually causes myofibre damage. Non-genetic muscle disorders, including sarcopenia, diabetes, bedridden immobility and cancer cachexia, lead to...

journal_title：Journal of biochemistry

authors： Fukada SI

更新日期：2018-05-01 00:00:00

abstract：:Alginate, which is an anionic polysaccharide, is widely distributed in the cell wall of brown algae. Alginate and the products of its degradation (oligosaccharides) are used in stabilizers, thickeners and gelling agents, especially in the food industry. The degradation of alginate generally involves a combination of s...

journal_title：Journal of biochemistry

authors： Yagi H,Fujise A,Itabashi N,Ohshiro T

更新日期：2018-04-01 00:00:00