Cell surface chondroitin sulphate proteoglycan 4 (CSPG4) binds to the basement membrane heparan sulphate proteoglycan, perlecan, and is involved in cell adhesion.

abstract：:ATP-dependent enzymes were investigated as to the stringency of their ATP requirement. For all the enzymes examined except firefly luciferase (including hexokinase, polynucleotide kinase, T4 DNA ligase, and T4 RNA ligase) ATP could be replaced with dATP, contradicting previous data. Considering the replaceable nucleot...

journal_title：Journal of biochemistry

authors： Kinoshita Y,Nishigaki K

更新日期：1997-07-01 00:00:00

abstract：:Dynamic functionality of mitochondria is maintained by continual fusion and fission events. A mitochondrial outer membrane protein Fzo1 plays a pivotal role upon mitochondrial fusion by homo-oligomerization to tether fusing mitochondria. Fzo1 is tightly regulated by ubiquitylations and the ubiquitin-responsible AAA pr...

journal_title：Journal of biochemistry

authors： Nahar S,Chowdhury A,Ogura T,Esaki M

更新日期：2020-03-01 00:00:00

abstract：:The nicotinamide adenine dinucleotide (NADH)/nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and the xanthine oxidase (XOD) systems generate reactive oxygen species (ROS). In the present study, to characterize the difference between the two systems, the kinetics of ROS generated by both the NADH oxidase an...

journal_title：Journal of biochemistry

authors： Sato E,Mokudai T,Niwano Y,Kohno M

更新日期：2011-08-01 00:00:00

abstract：:A novel esterase was found in Pseudomonas fluorescens cells and purified to homogeneity as determined by polyacrylamide gel electrophoresis. The esterase was extracted from the cells by freeze-thawing and hypotonic treatment. Purification was achieved by ammonium sulfate precipitation, followed by successive chromatog...

journal_title：Journal of biochemistry

authors： Nakagawa A,Tsujita T,Okuda H

更新日期：1984-04-01 00:00:00

abstract：:The dopamine (DA)-translocating mechanism of synaptic vesicles isolated from rat brain has been studied in the presence of an artificially imposed delta pH on the vesicle membranes (acidic inside with respect to the external medium) without the aid of ATP-Mg2+. Under the experimental conditions, [3H]DA uptake by the s...

journal_title：Journal of biochemistry

authors： Tsudzuki T

更新日期：1984-06-01 00:00:00

abstract：:Monoclonal antibodies were raised against a synthetic peptide (43 amino acid residues) that corresponds to the complete profragment of human prorenin. Seven monoclonal antibodies were chosen for further characterization. Two antibodies, 2-X-C1 and 4-X-E1, reacted with the middle region and C-terminus of the profragmen...

journal_title：Journal of biochemistry

authors： Ishizuka Y,Iizumi S,Akiyama F,Hori H,Poorman RA,Lobl TJ,Murakami K

更新日期：1989-09-01 00:00:00

abstract：:We have cloned a DNA from a human pancreatic cDNA library using a cloned rat pancreatic elastase 1 cDNA as a probe, and determined its nucleotide sequence. This cDNA contains a coding region of 810 nucleotides which encodes a 270-amino-acid protein. The deduced amino acid sequence shows less than 60% homologies with r...

journal_title：Journal of biochemistry

authors： Shirasu Y,Takemura K,Yoshida H,Sato Y,Iijima H,Shimada Y,Mikayama T,Ozawa T,Ikeda N,Ishida A

更新日期：1988-08-01 00:00:00

abstract：:The 565 amino-acid PRIB protein with a Zn(II)2Cys6 zinc-cluster DNA-binding motif is the expression product of the priB gene, which is most actively transcribed in an early stage of fruiting-body formation by the basidiomycete, Lentinus edodes. PRIB produced in Escherichia coli using the bacteriophage T7 expression sy...

journal_title：Journal of biochemistry

authors： Miyazaki Y,Tsunoka O,Shishido K

更新日期：1997-12-01 00:00:00

abstract：:The complete amino acid sequence of acylphosphatase from rabbit skeletal muscle has been elucidated by automatic Edman degradation of peptides obtained from staphylococcal protease and trypsin digestions. The enzyme consisted of a single polypeptide chain of 98 amino acid residues, lacking only histidine. Its amino (N...

journal_title：Journal of biochemistry

authors： Kizaki T,Takasawa T,Mizuno Y,Shiokawa H

更新日期：1985-04-01 00:00:00

abstract：:The effect of extracellular type-II phospholipase A2 (PLA2) on prostaglandin (PG) synthesis has been studied using rat liver-derived BRL-3A cells. The addition of type-II PLA2 to the medium of BRL-3A cells resulted in a marked decrease in the enzymatic activity in the medium. An immunochemical study involving an anti-...

journal_title：Journal of biochemistry

authors： Suga H,Murakami M,Kudo I,Inoue K

更新日期：1995-11-01 00:00:00

abstract：:Aspartate aminotransferase (AspAT), responsible for a minor part of the total AspAT enzymic activity in alkalophilic Bacillus circulans, was purified, its N-terminal amino acid sequence was determined, and its gene was cloned as two separate fragments. DNA sequencing showed an open reading frame of 432 amino acids (M(...

journal_title：Journal of biochemistry

authors： Battchikova N,Koivulehto M,Denesyuk A,Ptitsyn L,Boretsky Y,Hellman J,Korpela T

更新日期：1996-08-01 00:00:00

abstract：:Rat serum carboxylesterase [carboxylic ester hydrolase, EC 3.1.1.1] was purified by ammonium sulfate precipitation, chromatography on DEAE-cellulose, QAE Sephadex A-50 and brushite, and gel filtration on Sephadex G-200. This purified enzyme was shown to be a single protein band on slab gel electrophoresis and its fina...

journal_title：Journal of biochemistry

authors： Hashinotsume M,Higashino K,Hada T,Yamamura Y

更新日期：1978-12-01 00:00:00

abstract：:The rates of synthesis and degradation of arginosuccinate synthetase in rat liver under various dietary conditions were determined. The relative rate of the enzyme synthesis in the livers of rats fed on 70% casein diet was 4.0 times greater than that for rats fed on 5% casein diet. The rate constants of degradation (K...

journal_title：Journal of biochemistry

authors： Tsuda M,Shikata Y,Katsunuma T

更新日期：1979-03-01 00:00:00

abstract：:We previously reported that sera from various kinds of animals contain a protein(s) capable of inhibiting the growth of the non-malignant epithelial cell line derived from Buffalo rat liver (BRL). In the present study, a similar epithelial cell-specific growth inhibitor (EGI) was purified to homogeneity from an acid-e...

journal_title：Journal of biochemistry

authors： Huang W,Kimura T,Mashima K,Miyazaki K,Masaki H,Yamashita J,Horio T

更新日期：1986-09-01 00:00:00

abstract：:We have developed a novel method for the preparation of 'recombinant proteoliposomes'. Membrane proteins were expressed on budded virus (BV) envelopes using baculovirus gene expression systems, and proteoliposomes were prepared by fusion of these viruses with liposomes. First, plasmid DNA containing the gene for the t...

journal_title：Journal of biochemistry

authors： Fukushima H,Mizutani M,Imamura K,Morino K,Kobayashi J,Okumura K,Tsumoto K,Yoshimura T

更新日期：2008-12-01 00:00:00

abstract：:The activity and stability of mushroom tyrosinase were studied in ionic liquid (IL)-containing aqueous systems. The effect of three ILs ([BMIm][PF(6)], [BMIm][BF(4)]), and [BMIm][MeSO(4)], where [BMIm] = 1-butyl-3-methylimidazolium) and their inorganic salts (KMeSO(4), KPF(6), and NaBF(4)) on the enzyme performance wa...

journal_title：Journal of biochemistry

authors： Yang Z,Yue YJ,Huang WC,Zhuang XM,Chen ZT,Xing M

更新日期：2009-03-01 00:00:00

abstract：:The gene encoding a MutM from Desulfovibrio vulgaris (Miyazaki F) was cloned and expressed in Escherichia coli. A 5.9-kb DNA fragment, isolated from D. vulgaris (Miyazaki F) by XhoI and PvuII, contained a MutM gene and other open reading frames. The nucleotide sequence of the MutM gene indicated that the protein was c...

journal_title：Journal of biochemistry

authors： Sanada H,Nakanishi T,Inoue H,Kitamura M

更新日期：2009-04-01 00:00:00

abstract：:Ion-exchange chromatography (IEC) is the most frequently used chromatographic technique for the separation of proteins and peptides. In this article, the effects of urea on IEC separation of kiwifruit actinidin, egg white and urinary proteins were examined. The purity and relative amount of each protein in different c...

journal_title：Journal of biochemistry

authors： Khademi F,Mostafaie A

更新日期：2010-05-01 00:00:00

abstract：:Large amounts of extra proteins, X (in the envelope fraction) and X' (in the cytoplasmic fraction) were detected by SDS-polyacrylamide gel electrophoresis when DNA of Escherichia coli was damaged. These two proteins had the same apparent molecular weight (appproximately 40,000) and were produced under identical condit...

journal_title：Journal of biochemistry

authors： Yoda K,Nagai K,Tamura G

更新日期：1977-05-01 00:00:00

abstract：:The subcellular distribution of calmodulin and particulate calmodulin-binding activity was studied in a eukaryotic protozoan, Tetrahymena pyriformis NT-1. The particulate calmodulin-binding activity was found to be localized principally in microsomes and to some extent in cilia and surface membranes called pellicles. ...

journal_title：Journal of biochemistry

authors： Nagao S,Banno Y,Nozawa Y,Sobue K,Yamazaki R,Kakiuchi S

更新日期：1981-09-01 00:00:00

abstract：:Several kinds of hydrophilic proteins were examined to determine their interaction with artificial liposomes. Mitochondrial aspartate aminotransferase (m-GOT) [EC 2.6.1.1], as well as cytochrome c, was found to interact strongly with negatively charged liposomes. In each case, an appreciable amount of the protein boun...

journal_title：Journal of biochemistry

authors： Furuya E,Yoshida Y,Tagawa K

更新日期：1979-05-01 00:00:00

abstract：:The activity of biological molecules is often affected by their phosphorylation state. Regulatory phosphorylation operates as a binary switch and is usually controlled by counteracting kinases and phosphatases. However, phosphatidylinositol (PtdIns) has three phosphorylation sites on its inositol ring. The phosphoryla...

journal_title：Journal of biochemistry

authors： Miyazawa K

更新日期：2013-01-01 00:00:00

abstract：:In regenerating rat liver, nuclear protein histone H2A was shown to be phosphorylated on its amino-terminal serine residue [Sung et al. (1971) J. Biol. Chem. 246, 1358-1364], but the protein kinase which phosphorylates this residue has not been identified. To evaluate the possibility that protein kinase C can phosphor...

journal_title：Journal of biochemistry

authors： Takeuchi F,Hashimoto E,Yamamura H

更新日期：1992-06-01 00:00:00

abstract：:Pyruvate decarboxylase [2-oxo acid carboxy-lyase, EC 4.1.1.1] was isolated from sweet potato roots and was partially purified from healthy and diseased tissues. There was no appreciable difference in properties between the enzymes from healthy and diseased tissues. The molecular weight of the enzyme was found to be 24...

journal_title：Journal of biochemistry

authors： Oba K,Uritani I

更新日期：1975-06-01 00:00:00

abstract：:The activity of thermolysin is greatly enhanced in the presence of high concentrations of neutral salts [Holmquist, B. and Vallee, B.L. (1976) Biochemistry 15, 101-107; Inouye, K. (1992) J. Biochem. 112, 335-340]. NaBr and NaCl are the most effective for the activation. An absorption difference spectrum with a peak ar...

journal_title：Journal of biochemistry

authors： Inouye K,Kuzuya K,Tonomura B

更新日期：1994-09-01 00:00:00

abstract：:We developed a method to quantify galectin-7 extracted from adult mouse tissues by Western blot analysis. More than 0.5 ng of galectin-7 per mg of tissue was detectable by this method. The amounts of galectin-7 in tissues were determined as follows: skin, 62 +/- 3 ng/mg; esophagus, 23 +/- 8 ng/mg; stomach, 18 +/- 6 ng...

journal_title：Journal of biochemistry

authors： Sato M,Nishi N,Shoji H,Kumagai M,Imaizumi T,Hata Y,Hirashima M,Suzuki S,Nakamura T

更新日期：2002-02-01 00:00:00

abstract：:Four glycolipids (Fr. I, II, III, and IV) were isolated from whale brain and three (Fr. I, II, and III) of them were demonstrated to be galactosyl ceramide combined with fatty acid by ester linkage. Fr. IV was shown to be a monoalkyl monoacyl galactosyl glycerol. For determining the substituted position of the acyl gr...

journal_title：Journal of biochemistry

authors： Yasugi E,Saito E,Kasama T,Kojima H,Yamakawa T

更新日期：1982-04-01 00:00:00

abstract：:Phase transition was detected by a fluorescence polarization technique in the membrane of Thermus thermophilus HB8: it was found to be a function of cell growth temperature when the cell growth temperature was varied between 50-80 degrees C. A systematic relation between the phase transition temperature and the growth...

journal_title：Journal of biochemistry

authors： Wakayama N,Oshima T

更新日期：1978-06-01 00:00:00

abstract：:Neutral ceramidase (NCDase) is considered to be a critical enzyme for controlling the turnover of ceramide, an important bioactive lipid, which determines cell's fate. All-trans retinoic acid (ATRA) has been reported to induce neuronal differentiation and cell-cycle arrest [Lopez-Carballo, Moreno, Masia, Perez, and Ba...

journal_title：Journal of biochemistry

authors： Tanaka K,Tamiya-Koizumi K,Hagiwara K,Ito H,Takagi A,Kojima T,Suzuki M,Iwaki S,Fujii S,Nakamura M,Banno Y,Kannagi R,Tsurumi T,Kyogashima M,Murate T

更新日期：2012-06-01 00:00:00

abstract：:A post-proline cleaving enzyme [post-proline endopeptidase: EC 3.4.21.26] was purified from lamb brain by a series of column chromatographies on DEAE-Sephadex, hydroxyapatite and Sephadex G-150. The purified enzyme appeared homogeneous on disc gel and sodium dodecyl sulfate (SDS) gel electrophoreses. The enzyme was mo...

journal_title：Journal of biochemistry

authors： Yoshimoto T,Simmons WH,Kita T,Tsuru D

更新日期：1981-08-01 00:00:00