Abstract：
:The rates of synthesis and degradation of arginosuccinate synthetase in rat liver under various dietary conditions were determined. The relative rate of the enzyme synthesis in the livers of rats fed on 70% casein diet was 4.0 times greater than that for rats fed on 5% casein diet. The rate constants of degradation (Kd of argininosuccinate synthetase were estimated to be 0.15 and 0.16 day-1 under 70% and 5% casein feeding, respectively. When the dietary conditions were changed acutely from 70% to 5% casein diet or vice versa, the rates of the enzyme synthesis decreased or increased, respectively, and the rates of enzyme degradation were also affected. The change from 5% to 70% casein diet caused a transient decrease in the rate of degradation. After the enzyme activity had achieved a new steady-state level, the enzyme degradation proceeded at the normal steady rate. On the other hand, the change from 70% to 5% casein diet caused a transient increase in the rate of degradation. Thus, the only factor regulating the amount of enzyme in rat liver is the rate of enzyme synthesis under the steady-state conditions. However, the rates of both enzyme synthesis and degradation are involved in the regulation of the amount of enzyme during dietary transition.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Tsuda M,Shikata Y,Katsunuma Tsubject
Has Abstractpub_date
1979-03-01 00:00:00pages
699-704issue
3eissn
0021-924Xissn
1756-2651journal_volume
85pub_type
杂志文章abstract：:Many membrane proteins such as ion channels are oligomers, but the determinants of the degree of oligomerization are not fully understood. Mechanosensitive channel with large conductance (MscL), which is ubiquitous in bacteria, is a homopentamer with two transmembrane helices and a cytoplasmic helix in each subunit. T...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvv019
更新日期：2015-08-01 00:00:00
abstract：:The substrate specificity of microbial transglutaminase (MTG) from Streptomyces mobaraensis (formerly categorized Streptoverticillium) was studied using a Streptomyces proteinaceous protease inhibitor, STI2, as a model amine-donor substrate. Chemical modification and mutational analysis to address the substrate requir...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a022769
更新日期：2000-09-01 00:00:00
abstract：:The complete amino acid sequences of the Ulex europeus anti-H(O) lectins I and II were determined by using a protein sequencer. After digestion with endoproteinases Lys-C and Asp-N of the lectins reduced with 2-mercaptoethanol and modified with iodoacetamide, the resulting peptides were purified by reversed-phase high...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a123435
更新日期：1991-04-01 00:00:00
abstract：:Peroxiredoxin from Pyrococcus horikoshii (PhPrx) is a decameric protein formed by ring-type assembly of five dimers. To engineer the quaternary structure of PhPrx, we created a mutant PhPrx (PhPrx6m) by introducing six point mutations designed to dissociate PhPrx into dimers. Although PhPrx6m was a dimer in solution, ...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvx045
更新日期：2017-12-01 00:00:00
abstract：:Maitotoxion, a putative Ca2+ channel agonist, was isolated from cultures of the marine dinoflagellate Gambierdiscus toxicus as a colorless amorphous solid. The toxin reacted positively to Dragendorff's reagent but not to ninhydrin reagent. The mouse lethality of maitotoxin determined by intraperitoneal injection was 0...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a122438
更新日期：1988-08-01 00:00:00
abstract：:Three radioactive glycopeptides were isolated from human peripheral lymphocytes stimulated with Wistaria floribunda mitogen in the presence of D-[14C]glucosamine hydrochloride by mild trypsin digestion followed by gel filtration and preparative high-voltage paper electrophoresis. The carbohydrate compositions of these...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
abstract：:In order to characterize the mechanism for bilirubin transport in the liver, the uptake of bilirubin diglucuronide (BDG) into purified sinusoidal plasma membrane vesicles was investigated. BDG uptake was saturable, and was inhibited by sulfobromophthalein and unconjugated bilirubin, but was not affected by sodium taur...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a123120
更新日期：1990-05-01 00:00:00
abstract：:Pork insulin was subjected to mercaptosuccinylation and then coupled to beta-D-galactosidase [EC 3.2.1.23] from Escherichia coli using N,N'-o-phenylenedimaleimide. The competitive binding of the conjugate and insulin to anti-insulin antibody was tested. Results showed that formation of an insulin-beta-D-galactosidase ...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：
更新日期：1975-07-01 00:00:00
abstract：:The phi X174-DNA membrane complex was isolated from Escherichia coli infected with phi X174 am3 by isopycnic sucrose gradient centrifugation followed by zone electrophoresis. The phi X174 DNA-membrane complex banded at two positions, intermediate density membrane fraction and cytoplasmic membrane fraction, having bouy...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a132999
更新日期：1980-08-01 00:00:00
abstract：:The dissociation of porcine erythrocyte catalase [EC 1.11.1.6] into subunits on denaturation with alkali, GuHCl and urea was investigated by following the changes in hydrodynamic properties, absorption and CD spectra in the Soret region and inactivation of the enzyme. It was found that dissociation proceeded in an "al...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134252
更新日期：1983-04-01 00:00:00
abstract：:Phospholipase A2 of rat liver mitochondria was purified approximately 1,400-fold by extraction with KCl, and chromatographies on a Sephadex G-75 column and a diacyl-glycerophosphocholine-Sepharose affinity column. The purified enzyme was very labile when incubated either at 37 degrees C or 0 degrees C, and lost its ac...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134219
更新日期：1983-02-01 00:00:00
abstract：:The effects of sphingoid bases, sphingosine and dihydrosphingosine, which are protein kinase C (PKC) inhibitors, on NADPH oxidase were examined in a cell-free system. The bases inhibited cell-free activation of NADPH oxidase by arachidonic acid at lower concentration than N-acetylsphingosine. Thus, positive charge in ...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a021468
更新日期：1996-10-01 00:00:00
abstract：:Myosin subfragment-1.pyrophosphate (S1.PPi) complex and S1.ADP complex were observed with 31P NMR at various temperatures between 0 and 25 degrees C. The signal of S1.PPi complex showed a small temperature dependence, indicating that a single conformation exists for the complex. It also showed that the electron densit...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a123996
更新日期：1993-01-01 00:00:00
abstract：:Acridine orange was found to accumulate in pure lysosomal particles (tritosomes) in vitro, and the quenching of its fluorescence correlated well with the delta pH (inside acid) across the lysosomal membrane. Use of this dye showed that Mg-ATP caused lysosomal acidification. This acidification was sensitive to N,N'-dic...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134053
更新日期：1982-10-01 00:00:00
abstract：:Homogeneous indanol dehydrogenase from monkey liver catalyzed the reversible conversion of 3 alpha- or 20 alpha-hydroxy groups of several bile acids and 5 beta-pregnanes to the corresponding 3- or 20-ketosteroids. The kcat values for the steroids determined at pH 7.4 were low, but the kcat/Km values for the 3-ketoster...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a122949
更新日期：1989-11-01 00:00:00
abstract：:It was previously found that rabbit serum contains a growth-inhibitory substance for a tumorigenic rat liver cell line RSV-BRL. In the present study, the growth inhibitor was purified from normal rabbit serum to show a homogeneous protein band with a molecular weight (Mr) of 56 k on SDS-polyacrylamide gel electrophore...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a123597
更新日期：1991-09-01 00:00:00
abstract：:Despite the usefulness of the patch-clamp technique, its application to ion pumps and transporters in biomembranes is limited. We developed a novel method for determining the activity of a proton-pumping pyrophosphatase (H(+)-PPase) made of a single protein. We heterologously highly expressed the enzyme in Saccharomyc...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvg184
更新日期：2003-10-01 00:00:00
abstract：:In this report, we describe a laser-latex combination system that enables membrane-impermeable molecules to penetrate cell membranes. Laser light (Q-switched Nd:YAG laser, 532.5 nm) was used to irradiate a mixture of commercial latex particles (blue dyed, 1 micro m in diameter) and mouse fibrosarcoma (Meth-A) cells. A...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvg132
更新日期：2003-08-01 00:00:00
abstract：:A form of UDP-glucuronyltransferase has been purified from liver microsomes of 3-methylcholanthrene-treated rats by a simple and rapid method involving chromatography on DEAE-Toyopearl and UDP-hexanolamine Sepharose columns. The purified preparation gave a single protein band (Mr 54,000) on sodium dodecyl sulfate-poly...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a122505
更新日期：1988-10-01 00:00:00
abstract：:Studies on human cell hybrids of a cervical carcinoma cell line, HeLa, and normal fibroblasts have indicated that the tumorigenicity of these cells is under the control of a putative tumor suppressor on chromosome 11, although the nature of this suppressor remains unknown. We examined the expression of caveolin-1, a p...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a022123
更新日期：1998-08-01 00:00:00
abstract：:Four distinctive DNA polymerases have been isolated from mature oocytes of the starfish, Asterina pectinifera, and characterized. The N-ethylmaleimide-resistant DNA polymerase activity was separated from the other three DNA polymerases by hydroxyapatite column chromatography. The enzyme, whose approximate sedimentatio...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/93.3.687
更新日期：1983-03-01 00:00:00
abstract：:Increased fructose concentration in erythrocytes of diabetic patients subject haemoglobin (Hb) to be glycated by fructose. Haemoglobin glycation results in early and advanced glycation end products which are known as HbA(1c) and Hb-AGE, respectively. In diabetics the Hb-AGE content raises and the mean parameter multip...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvm096
更新日期：2007-06-01 00:00:00
abstract：:The Ca2+-ATPase of sarcoplasmic reticulum from rabbit skeletal muscle was incorporated into vesicles made from dimyristoylphosphatidylcholine or dipalmitoylphosphatidylcholine. The Ca2+-ATPase activity of these reconstituted membranes became appreciable above 20 degrees C and 30 degrees C, respectively, in accord with...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134528
更新日期：1983-12-01 00:00:00
abstract：:The minimal structural unit of cytochrome c oxidase purified from Thiobacillus novellus was composed of one molecule each of two subunits with molecular masses of 32 and 23 kDa, respectively, and the unit had one molecule of heme a and one atom of copper. In the presence of n-octyl-beta-D-thioglucoside, the oxidase ex...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a123717
更新日期：1992-01-01 00:00:00
abstract：:Aggregatibacter actinomycetemcomitans is an oral pathogen for aggressive periodontitis, and encodes a triheme c-containing membrane-bound enzyme, quinol peroxidase (QPO) that catalyzes peroxidase activity using quinol in the respiratory chain. In the previous work, we have characterized recombinant QPO purified from t...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvw099
更新日期：2017-06-01 00:00:00
abstract：:We studied the effect of Ca2+ on the reactivities of two SH groups of DTNB light chain (Cys 128 and Cys 157) using a fluorogenic thiol reagent. It was found that a Ca2+-induced change in reactivity occurred only with Cys 128 when the light chain was in an isolated state, whereas it occurred with both Cys 128 and Cys 1...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a132727
更新日期：1980-01-01 00:00:00
abstract：:Cytochromes b(561) are a family of transmembrane proteins found in most eukaryotic cells and contain two haem b prosthetic groups per molecule being coordinated with four His residues from four different transmembrane alpha-helices. Although cytochromes b(561) residing in the chromaffin vesicles has long been known to...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvm181
更新日期：2007-11-01 00:00:00
abstract：:The ascorbate oxidase obtained from a microorganism, Acremonium sp. HI-25 (molecular weight, 80 kDa; monomeric protein), was studied with respect to atomic absorption, EPR, absorption spectra, circular dichroism (CD) spectra, and steady-state kinetics. The enzyme was found to be a multicopper protein, containing four ...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a124420
更新日期：1994-05-01 00:00:00
abstract：:Haematopoiesis and blood vessel formation are closely associated, with several molecules employed by both systems. Recently, vasohibin-1 (VASH1), an endothelium-derived negative feedback regulator of angiogenesis, has been isolated and characterized. VASH1 is induced by VEGF or bFGF in endothelial cells (ECs) and inhi...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvp021
更新日期：2009-05-01 00:00:00
abstract：:Escherichia coli spr (suppressor of prc) mutants and nlpI mutants show thermosensitive growth. The thermosensitivity of the spr mutants was suppressed by the nlpI mutations. Expression of the fusion genes encoding hexa-histidine-tagged NlpI (NlpI-His) and purification of the tagged NlpI showed that NlpI-His bound with...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvh022
更新日期：2004-02-01 00:00:00
