Abstract:
:In this report, we describe a laser-latex combination system that enables membrane-impermeable molecules to penetrate cell membranes. Laser light (Q-switched Nd:YAG laser, 532.5 nm) was used to irradiate a mixture of commercial latex particles (blue dyed, 1 micro m in diameter) and mouse fibrosarcoma (Meth-A) cells. After irradiation, membrane permeability was evaluated by flow cytometric assaying using propidium iodide (PI) and fluorescein diacetate (FDA). The proportion of permeabilized-resealed cells was affected by changes in the light intensity (approximately 780 mW/cm(2)), the irradiation time (approximately 240 s), and/or the particle concentration (approximately 10(9) particles/ml). The permeability persisted up to 20 min after light irradiation. Near the sites of individual particles, the permeability of the cell membrane is modified, probably due to localized temperature changes. These results suggest that this laser-induced permeabilization strategy constitutes a new means of delivering exogenous materials into living cells.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Umebayashi Y,Miyamoto Y,Wakita M,Kobayashi A,Nishisaka Tdoi
10.1093/jb/mvg132keywords:
subject
Has Abstractpub_date
2003-08-01 00:00:00pages
219-24issue
2eissn
0021-924Xissn
1756-2651journal_volume
134pub_type
杂志文章abstract::Here we report the enzymatic and ligand-binding properties of a major secretory protein in the choroid plexus of cane toad, Bufo marinus, whose protein is homologous with lipocalin-type prostaglandin (PG) D synthase (L-PGDS) and is recombinantly expressed in Xenopus A6 cells and Escherichia coli. The toad protein boun...
journal_title:Journal of biochemistry
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133365
更新日期:1981-05-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a121985
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pub_type: 杂志文章,评审
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更新日期:2018-03-01 00:00:00
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更新日期:1999-10-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134301
更新日期:1983-06-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133259
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133798
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133713
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
abstract::We studied the roles of cofilin, an actin-binding phosphoprotein, in superoxide production of neutrophil-like HL-60 cells triggered by opsonized zymosan (OZ). OZ caused dephosphorylation of cofilin as well as a transient increase of F-actin. Both reactions were complete within 30 s. Okadaic acid (OA) magnified the OZ-...
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132254
更新日期:1978-12-01 00:00:00
abstract::Eukaryotic typical 2-Cys type peroxiredoxin (Prx) is inactivated by hyperoxidation of the peroxidatic cysteine to a sulphinic acid in a catalytic cycle-dependent manner. This inactivation process has been well documented for cytosolic isoforms of Prx. However, such a hyperoxidative inactivation has not fully been inve...
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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journal_title:Journal of biochemistry
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pub_type: 杂志文章
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abstract::To clarify the relationship among ryanodine receptor isoforms from skeletal muscles of various vertebrates (alpha- and beta-isoforms with smaller and larger mobilities on SDS-polyacrylamide gel electrophoresis, respectively), we examined the cross-reactivities of those receptors from 13 animal species by Western blot ...
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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abstract::Murine interleukin-2 (MIL-2) cDNA deleted of 11 repeats of a CAG sequence, and that encoded from 33Met to 149Gln were inserted into an expression vector carrying an Escherichia coli tryptophan promoter and were expressed in E. coli, respectively. Recombinant MIL-2 deleted of 11 glutamine repeats (MIL-2(-Gln] supported...
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