Regulation of AMP deaminase from chicken erythrocytes. A kinetic study of the allosteric interactions.

abstract：:The substrate specificity of microsomal nonspecific carboxyesterase [EC 3.1.1.1] from rabbit and rat livers was studied in vitro by using seven (A)-type and six (B)-type 12,13-epoxytrichothecene mycotoxins. The C-4 acetyl residues of diacetoxyscirpenol, T-2 toxin, monoacetylnivalenol (fusarenon-X), and diacetylnivalen...

journal_title：Journal of biochemistry

authors： Ohta M,Matsumoto H,Ishii K,Ueno Y

更新日期：1978-09-01 00:00:00

abstract：:Escherichia coli spr (suppressor of prc) mutants and nlpI mutants show thermosensitive growth. The thermosensitivity of the spr mutants was suppressed by the nlpI mutations. Expression of the fusion genes encoding hexa-histidine-tagged NlpI (NlpI-His) and purification of the tagged NlpI showed that NlpI-His bound with...

journal_title：Journal of biochemistry

authors： Tadokoro A,Hayashi H,Kishimoto T,Makino Y,Fujisaki S,Nishimura Y

更新日期：2004-02-01 00:00:00

abstract：:We developed a high-throughput Enzyme-linked immunosorbent assay (ELISA) for measuring α2,6-sialylated transferrin (Tf), based on inhibition of anti-Tf antibody binding to α2,6-sialylated Tf by a lectin, Sambucus sieboldiana Agglutinin (SSA). The inhibition was not observed with other glycoforms, such as periodate-tre...

journal_title：Journal of biochemistry

authors： Hoshi K,Kariya Y,Nara K,Ito H,Matsumoto K,Nagae M,Yamaguchi Y,Nakajima M,Miyajima M,Arai H,Kuno A,Narimatsu H,Shirotani K,Hashimoto Y

更新日期：2013-09-01 00:00:00

abstract：:In this paper we speculate on the origin and early evolution of transition element enzymes. Iron, molybdenum, and zinc, the most abundant transition elements in seawater, presumably complexed with compounds accumulated in the primeval sea in the course of chemical evolution forming compounds with subsequently evolved ...

journal_title：Journal of biochemistry

authors： Egami F

更新日期：1975-06-01 00:00:00

abstract：:Cathepsin E, an intracellular aspartic proteinase of the pepsin family, is composed of two homologous domains, each containing the catalytic Asp residue in a consensus DTG motif. Here we examine the significance of residues in the motifs of rat cathepsin E by substitution of Asp98, Asp283, and Thr284 with other residu...

journal_title：Journal of biochemistry

authors： Liu J,Tsukuba T,Okamoto K,Ohishi M,Yamamoto K

更新日期：2002-09-01 00:00:00

abstract：:The virion-associated RNA-dependent RNA polymerase of influenza virus PR8 was activated by treatment with specific non-ionic detergents, and was remarkably stimulated by exogenously added oligonucleotide primers. Among synthetic oligonucleotides of various chain lengths, the dinucleotide ApG and the trinucleotide ApGp...

journal_title：Journal of biochemistry

authors： Kawakami K,Ishihama A,Ohtsuka E,Tanaka T,Takashima H,Ikehara M

更新日期：1981-06-01 00:00:00

abstract：:A gene for a methionine aminopeptidase (MAP; EC 3.4.11.18), which catalyzes the removal of amino-terminal methionine from the growing peptide chain on the ribosome, has been cloned from the hyperthermophilic Archaeon, Pyrococcus furiosus, by a novel method effectively using its cosmid protein library, sequenced and ex...

journal_title：Journal of biochemistry

authors： Tsunasawa S,Izu Y,Miyagi M,Kato I

更新日期：1997-10-01 00:00:00

abstract：:ISG15 is a ubiquitin-like protein that is upregulated on treatment with interferon. ISG15 is considered to be covalently conjugated to cellular proteins through a sequential reaction similar to that of the ubiquitin conjugation system consisting of E1/E2/E3 enzymes: UBE1L and UbcH8 have been reported to function as E1...

journal_title：Journal of biochemistry

authors： Takeuchi T,Iwahara S,Saeki Y,Sasajima H,Yokosawa H

更新日期：2005-12-01 00:00:00

abstract：:Anti-cobrotoxin antibodies can be separated into precipitin and non-precipitin antibodies. The precipitin antibody possesses the same binding affinity to cobrotoxin as non-precipitin antibody, but the neutralizing capability of the latter is superior to that of the former in blocking cobrotoxin binding to nicotinic ac...

journal_title：Journal of biochemistry

authors： Kuo KW,Chang LS,Lin PM,Leaber RJ,Chang CC

更新日期：1994-12-01 00:00:00

abstract：:Methioninase of Pseudomonas putida was purified to homogeneity, as judged by polyacrylamide gel electrophoresis, with a specific activity 270-fold higher than that of the crude extract. 1. The purified enzyme had an S20,w of 8.37, a molecular weight of 160,000, and an isoelectric point of 5.6. 2. A break in the Arrhen...

journal_title：Journal of biochemistry

authors： Ito S,Nakamura T,Eguchi Y

更新日期：1976-06-01 00:00:00

abstract：:Four glycolipids (Fr. I, II, III, and IV) were isolated from whale brain and three (Fr. I, II, and III) of them were demonstrated to be galactosyl ceramide combined with fatty acid by ester linkage. Fr. IV was shown to be a monoalkyl monoacyl galactosyl glycerol. For determining the substituted position of the acyl gr...

journal_title：Journal of biochemistry

authors： Yasugi E,Saito E,Kasama T,Kojima H,Yamakawa T

更新日期：1982-04-01 00:00:00

abstract：:The modification of proteins by SUMO (small ubiquitin-like modifier) regulates various cellular processes. Sumoylation often occurs on a specific lysine residue within the consensus motif psiKxE/D. However, little is known about the specificity and selectivity of SUMO target sites. We describe here a SUMO assay with p...

journal_title：Journal of biochemistry

authors： Schwamborn K,Knipscheer P,van Dijk E,van Dijk WJ,Sixma TK,Meloen RH,Langedijk JP

更新日期：2008-07-01 00:00:00

abstract：:We have recently shown that furin, a mammalian homologue of the yeast precursor-processing endoprotease Kex2, is involved in precursor cleavage at sites marked by the Arg-X-Lys/Arg-Arg motif within the constitutive secretory pathway. In this study, we analyzed molecular and enzymatic properties of furin expressed in C...

journal_title：Journal of biochemistry

authors： Hatsuzawa K,Murakami K,Nakayama K

更新日期：1992-03-01 00:00:00

abstract：:Raman spectroscopy was used to investigate the hydrogen bonding at the C(4)=O moiety of the isoalloxazine nucleus in a series of flavins and flavoproteins. Isotope effects of Raman bands confirmed that the band observed around 1,710 cm(-1) is mainly derived from C(4)=O stretching vibrational mode. A linear correlation...

journal_title：Journal of biochemistry

authors： Hazekawa I,Nishina Y,Sato K,Shichiri M,Miura R,Shiga K

更新日期：1997-06-01 00:00:00

abstract：:Serologically active D-arabino-D-mannas ([alpha]D, +82 degrees approximately 89 degrees; ratio of D-arabinose to D-mannose, 1-2:1) were isolated from the soluble fraction of disintegrated cells of M. tuberculosis, M. smegmatis, and several other Mycobacterium species. These arabinomannans had similar structures, consi...

journal_title：Journal of biochemistry

authors： Misaki A,Azuma I,Yamamura Y

更新日期：1977-12-01 00:00:00

abstract：:Sliding machines composed of F-actin and myosin or microtubules and kinesin or dynein convert the free energy of ATP hydrolysis into sliding movements and mechanical work. Development of optical microscopy with micromanipulation techniques has made possible direct observation of single events of sliding exhibited by s...

journal_title：Journal of biochemistry

authors： Oosawa F

更新日期：1995-11-01 00:00:00

abstract：:A novel glycosphingolipid (SEGLx) has been isolated from the plerocercoids of a tapeworm, Spirometra erinacei. From the results of compositional analysis, methylation analysis, exoglycosidase hydrolysis, acid hydrolysis, fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance (NMR) analysis, its...

journal_title：Journal of biochemistry

authors： Kawakami Y,Nakamura K,Kojima H,Suzuki M,Inagaki F,Suzuki A,Sonoki S,Uchida A,Murata Y,Tamai Y

更新日期：1993-11-01 00:00:00

abstract：:The regulation of neutral cytosolic cholesterol ester hydrolase was studied in isolated rat liver cells. Addition of glucagon to cell suspensions caused a decrease in the enzyme activity which was significant at 1 nM concentration. The cyclic nucleotide analogue bibutyryl cyclic AMP (10 and 100 microM) also inhibited ...

journal_title：Journal of biochemistry

authors： Ruiz MB,Ochoa B,Lacort M

更新日期：1990-03-01 00:00:00

abstract：:The present investigation was undertaken to see to what extent the alpha-amino group of the amino acid, the side chain of the amino acid of aminoacyl-tRNA, and the tRNA structure are involved in determining the affinity of aminoacyl-tRNA for bacterial elongation factor Tu-GTP complex. Various aminoacyl-tRNAs, mis-amin...

journal_title：Journal of biochemistry

authors： Tanada S,Kawakami M,Nishio K,Takemura S

更新日期：1982-01-01 00:00:00

abstract：:A new hetero-bifunctional photo crosslinking reagent, 2-(4-azidoanilyl)-4-(4-azabicyclo-[2,2, 2]hexylammonio)-6-morpholino-1,3,5-triazine chloride, was designed to detect and isolate heparin-binding protein(s) that may act as heparin-receptor(s) on the platelet surface. In a preliminary study using ethanol as a model ...

journal_title：Journal of biochemistry

authors： Suda Y,Mori K,Bird K,Marques D,Ormsby J,Tanaka S,Koshida S,Nakamura M,Kusumoto S,Sobel M

更新日期：1999-06-01 00:00:00

abstract：:Sulfate-reducing bacteria, Desulfovibrio vulgaris, strain Miyazaki, were grown on either sulfate, sulfite, or thiosulfate as the terminal electron acceptor. Better growth was observed on sulfite and less growth on thiosulfate than on sulfate. Enzyme levels of adenylylsulfate (APS) reductase [EC 1.8.99.2], reductant-ac...

journal_title：Journal of biochemistry

authors： Kobayashi K,Morisawa Y,Ishituka T,Ishimoto M

更新日期：1975-11-01 00:00:00

abstract：:The crystal structure of an adenylate kinase from an extremophilic archaeon Aeropyrum pernix was determined in complex with full ligands, ATP-Mg2+ and AMP, at a resolution of 2.0 Å. The protein forms a trimer as found for other adenylate kinases from archaea. Interestingly, the reacting three atoms, two phosphorus and...

journal_title：Journal of biochemistry

authors： Shibanuma Y,Nemoto N,Yamamoto N,Sampei GI,Kawai G

更新日期：2020-09-01 00:00:00

abstract：:Fluorescence changes of 1-anilino-8-naphthalenesulfonic acid and 3,3'-dipropyl-2,2'-thiadicarbocyanine during Ca transport by sarcoplasmic reticulum vesicles were studied. The fluorescence of both probes is enhanced corresponding to the rapid initial Ca uptake. The enhancement could be interpreted in terms of increase...

journal_title：Journal of biochemistry

authors： Ueno T,Sekine T

更新日期：1978-10-01 00:00:00

abstract：:Corynebacterium diphtheriae, a pathogenic Gram-positive bacterium, contains sialic acids on its cell surface, but no genes related to sialic acid decoration or metabolism have been reported in C. diphtheriae. In the present study, we have identified a putative sialidase gene, nanH, from C. diphtheriae KCTC3075 and cha...

journal_title：Journal of biochemistry

authors： Kim S,Oh DB,Kwon O,Kang HA

更新日期：2010-04-01 00:00:00

abstract：:The structure of a ferricytochrome c' extracted from Rhodospirillum rubrum has been determined at 6 A resolution by the X-ray crystallographic method. The crystals, obtained by dialyzing the protein solution against polyethylene glycol 4000, belong to the hexagonal space group P6(1). Two heavy atom derivatives were ob...

journal_title：Journal of biochemistry

authors： Yasui M,Harada S,Kai Y,Kasai N

更新日期：1985-07-01 00:00:00

abstract：:Lipoamide dehydrogenase (LADase) was purified to homogeneity from rat liver mitochondria, and the intracellular distribution and biosynthesis of the LADase were investigated with antibody prepared against the purified enzyme. 1) LADase activity was mostly found in mitochondria; the activity in cytosol was about one-te...

journal_title：Journal of biochemistry

authors： Matuda S,Saheki T

更新日期：1982-02-01 00:00:00

abstract：:Oxidative activity of 9-anthraldehyde (9-AA) to 9-anthracenecarboxylic acid in monkey liver was mainly located in microsomes. The reaction required NADPH as an essential cofactor and was significantly inhibited by SKF 525-A, metyrapone, disulfiram, and CO, potent inhibitors of microsomal aldehyde oxygenase (MALDO). Tw...

journal_title：Journal of biochemistry

authors： Matsunaga T,Iwawaki Y,Watanabe K,Yamamoto I,Kageyama T,Yoshimura H

更新日期：1996-04-01 00:00:00

abstract：:Vasoactive intestinal peptide (VIP), a neuropeptide coupled with adenylate cyclase, was found to induce neurite extension of PC12h cells. Neurites appeared within 1 h after addition of VIP and extended for at least 24 h. The half-maximal concentration for the effect of VIP was 50 nM. In addition to the morphological c...

journal_title：Journal of biochemistry

authors： Okumura N,Miyatake Y,Takao T,Tamaru T,Nagai K,Okada M,Nakagawa H

更新日期：1994-02-01 00:00:00

abstract：:Tissue remodelling and regeneration in various pathophysiological conditions (e.g. the processes of development, pregnancy, inflammation, wound healing, tissue regeneration, tumor growth, etc.) require angiogenesis, a dynamically coordinated response to stimuli from the extracellular microenvironment. During angiogeni...

journal_title：Journal of biochemistry

authors： Sakaue T,Maekawa M,Nakayama H,Higashiyama S

更新日期：2017-10-01 00:00:00

abstract：:Previously, we showed that a polypeptide isolated from fetal bovine cartilage stimulates proteoglycan synthesis, RNA synthesis, and DNA synthesis in cultured rabbit costal chondrocytes. In the present study, the effects of the cartilage-derived factor (CDF) were compared with those of multiplication-stimulating activi...

journal_title：Journal of biochemistry

authors： Kato Y,Nomura Y,Tsuji M,Ohmae H,Nakazawa T,Suzuki F

更新日期：1981-11-01 00:00:00