Purification and characterization of methioninase from Pseudomonas putida.

abstract：:Genomic clones of UDP-glucuronosyltransferase family 1 (UGT1) were isolated from wild-type Wistar rats. The UGT1 locus spans > 120 kb and forms a gene complex. In this locus nine unique first exons encoding NH2-terminal portions of each isoform were located at intervals of approximately 10 kb and followed by only one ...

journal_title：Journal of biochemistry

authors： Emi Y,Ikushiro S,Iyanagi T

更新日期：1995-02-01 00:00:00

abstract：:Several proteins in the mammalian endoplasmic reticulum are substrates for protein kinases. Many unidentified phosphoproteins from this compartment are described in the literature, and this prompted us to try to identify at least the more dominant ones. When solubilized bovine and murine microsomes were phosphorylated...

journal_title：Journal of biochemistry

authors： Janson IM,Ek B,Ek P

更新日期：1997-01-01 00:00:00

abstract：:The receptor-binding assay for cAMP was improved by using polyethylenimine-treated glass filters. A polyethylenimine-treated glass filter has high protein binding capacity. This high capacity allows an increase in the amount of protein per assay tube and the use of a crude preparation, such as a beef heart extract, as...

journal_title：Journal of biochemistry

authors： Takeda T,Kuno T,Shuntoh H,Tanaka C

更新日期：1989-02-01 00:00:00

abstract：:1-O-Alk-1'-enyl-2-O-acetyl-glycerophosphocholine (vinyl form of PAF) was found with PAF in perfused rat and guinea pig hearts. The main molecular species of the vinyl form of PAF, after separation by reverse phase HPLC, were identified as 1-O-hexadec-, -octadec-, and -octadecen-1'-enyl-2-O-acetyl-GPCs (16:0, 18:0, and...

journal_title：Journal of biochemistry

authors： Nakayama R,Saito K

更新日期：1989-04-01 00:00:00

abstract：:We previously reported that DNAs directly applicable to restriction analyses and transformation of Escherichia coli can be extracted from fungi and yeasts by use of anhydrous hydrogen fluoride (HF) under a mild condition, 5 min at 0 degrees C [Oshiro, S., Katsura, N., Kitada, K., and Gunge, N. (1987) FEBS Lett. 220, 3...

journal_title：Journal of biochemistry

authors： Oshiro S,Katsura N

更新日期：1995-03-01 00:00:00

abstract：:We have reported that Erbin expression was down-regulated in the Jurkat leukaemia T lymphocytes treated with the recombinant soluble tumour necrosis factor-related apoptosis-inducing ligand (rsTRAIL). Herein, we studied the expression and the regulation of Erbin and its binding partner, ErbB2, in the MCF-7 breast canc...

journal_title：Journal of biochemistry

authors： Liu N,Zhang J,Zhang J,Liu S,Liu Y,Zheng D

更新日期：2008-06-01 00:00:00

abstract：:OsGEN-L has a 5'-flap endonuclease activity and plays an essential role in rice microspore development. The Class 4 RAD2/XPG family nucleases, including OsGEN-L, were recently found to have resolving activity for the Holliday junction (HJ), the intermediate of DNA strand recombination. In this study, we performed a de...

journal_title：Journal of biochemistry

authors： Yang Y,Ishino S,Yamagami T,Kumamaru T,Satoh H,Ishino Y

更新日期：2012-03-01 00:00:00

abstract：:A glutathione S-transferase isozyme which is absent in normal rat liver has been isolated from the hereditary hyperbilirubinuria rat liver cytosol. The enzyme was purified to apparent homogeneity by GSH-affinity chromatography and HPLC on CM-Sepharose CL-6B. It is a heterodimer of two non-identical subunits, i.e., sub...

journal_title：Journal of biochemistry

authors： Igarashi T,Tsuchiya T,Satoh T

更新日期：1991-01-01 00:00:00

abstract：:It was shown that a dipeptide, alanylhistidine, can act as a catalyst for the peptidyl transfer reaction in the absence of ribosomes between the amino acid moieties of phenylalanyl, lysyl, prolyl, and glycyl tRNAs depending on their model templates, poly U, poly A, poly C, and poly G, respectively. A template effect w...

journal_title：Journal of biochemistry

authors： Shimizu M

更新日期：1996-05-01 00:00:00

abstract：:The position in the acyl phosphate linkage of the phosphorylated intermediate of (Na+, K+)-ATPase that is cleaved by N-methylhydroxylamine was compared with that of the model compound acetylphosphate. The products of the cleavage of the phosphoenzyme by methylhydroxylamine were the active enzyme and a N-P compound, no...

journal_title：Journal of biochemistry

authors： Ushimaru M,Shinohara Y,Fukushima Y

更新日期：1997-09-01 00:00:00

abstract：:Here we report the enzymatic and ligand-binding properties of a major secretory protein in the choroid plexus of cane toad, Bufo marinus, whose protein is homologous with lipocalin-type prostaglandin (PG) D synthase (L-PGDS) and is recombinantly expressed in Xenopus A6 cells and Escherichia coli. The toad protein boun...

journal_title：Journal of biochemistry

authors： Irikura D,Inui T,Beuckmann CT,Aritake K,Schreiber G,Miyano M,Inoue T,Urade Y

更新日期：2007-02-01 00:00:00

abstract：:Microvesicles (MVs) serve as vectors of nucleic-acid dissemination and are important mediators of intercellular communication. However, the functionality of packaged nucleic acids on recipient cells following transfer of MV cargo has not been clearly elucidated. This limitation is attributed to a lack of methodology a...

journal_title：Journal of biochemistry

authors： Lu JF,Pokharel D,Padula MP,Bebawy M

更新日期：2016-11-01 00:00:00

abstract：:When meso-tetrakis(3-N-methylpyridiniumyl)porphyrin (m-TMPyP) formed a complex with poly[d(A-T)(2)], an intense bisignate excitonic CD in the Soret absorption region was observed. The excitonic CD of the m-TMPyP-poly[d(A-T)(2)] complex is unique in that no other combination of the related porphyrin, namely, meso-tetra...

journal_title：Journal of biochemistry

authors： Lee YA,Lee S,Lee HM,Lee CS,Kim SK

更新日期：2003-03-01 00:00:00

abstract：:We previously reported that caldesmon (CaD), together with tropomyosin (TM), effectively protects actin filaments from gelsolin, an actin-severing protein. To elucidate the structure/function relationship of CaD, we dissected the functional domain of CaD required for the protection. The basic C-terminal half of rat no...

journal_title：Journal of biochemistry

authors： Takiguchi K,Matsumura F

更新日期：2005-12-01 00:00:00

abstract：:In this report, we describe a laser-latex combination system that enables membrane-impermeable molecules to penetrate cell membranes. Laser light (Q-switched Nd:YAG laser, 532.5 nm) was used to irradiate a mixture of commercial latex particles (blue dyed, 1 micro m in diameter) and mouse fibrosarcoma (Meth-A) cells. A...

journal_title：Journal of biochemistry

authors： Umebayashi Y,Miyamoto Y,Wakita M,Kobayashi A,Nishisaka T

更新日期：2003-08-01 00:00:00

abstract：:Paranitrophenylphosphate (pNPP) induced fluorescence changes in fluorescence isothiocyanate (FITC)-labeled Na+,K(+)-ATPase preparations. The extents of changes were similar to those induced by acetylphosphate (AcP) accompanying accumulation of a K(+)-sensitive phosphoenzyme (E2P) and an ouabain bound phosphoenzyme in ...

journal_title：Journal of biochemistry

authors： Yamazaki A,Kaya S,Tsuda T,Araki Y,Hayashi Y,Taniguchi K

更新日期：1994-12-01 00:00:00

abstract：:Tetrahymena F-actin-binding protein, which induces bundling of Tetrahymena F-actin, was localized to a division furrow during cytokinesis. We report here the cloning and characterization of the gene and cDNA of a Tetrahymena F-actin-binding protein. The cDNA encodes a protein comprising 579 deduced amino acids with a ...

journal_title：Journal of biochemistry

authors： Watanabe A,Yonemura I,Gonda K,Numata O

更新日期：2000-01-01 00:00:00

abstract：:The complete amino acid sequence of ribonuclease N1 (RNase N1), a guanine-specific ribonuclease from a fungus, Neurospora crassa, was determined by conventional protein sequencing, using peptide fragments obtained by tryptic digestion of cyanogen bromide-treated RNase N1 and by Staphylococcus aureus V8 protease digest...

journal_title：Journal of biochemistry

authors： Takahashi K

更新日期：1988-09-01 00:00:00

abstract：:The human malaria parasite (Plasmodium falciparum) possesses a plastid-derived organelle called the apicoplast, which is believed to employ metabolisms crucial for the parasite's survival. We cloned and studied the biochemical properties of plant-type ferredoxin (Fd) and Fd-NADP+ reductase (FNR), a redox system that p...

journal_title：Journal of biochemistry

authors： Kimata-Ariga Y,Kurisu G,Kusunoki M,Aoki S,Sato D,Kobayashi T,Kita K,Horii T,Hase T

更新日期：2007-03-01 00:00:00

abstract：:Skeletal muscle is composed of multinuclear cells called myofibers. Muscular dystrophy (a genetic muscle disorder) induces instability in the cell membrane of myofibers and eventually causes myofibre damage. Non-genetic muscle disorders, including sarcopenia, diabetes, bedridden immobility and cancer cachexia, lead to...

journal_title：Journal of biochemistry

authors： Fukada SI

更新日期：2018-05-01 00:00:00

abstract：:The roles of adult worker honeybees change with age; young workers nurse brood by secreting bee milk (royal jelly), and older workers forage for nectar and pollen and process nectar into honey. The electrophoretic profile of worker hypopharyngeal-gland proteins changes with age and/or role. Immunoblotting analysis usi...

journal_title：Journal of biochemistry

authors： Kubo T,Sasaki M,Nakamura J,Sasagawa H,Ohashi K,Takeuchi H,Natori S

更新日期：1996-02-01 00:00:00

abstract：:Amino acid sequence analysis was done on a human lambda Bence Jones protein NIG-64 with the major objective of determining the sequence of the variable region. Nineteen tryptic peptides covering 216 residues were isolated from the completely reduced and aminoethylated protein, and 17 of these were completely sequenced...

journal_title：Journal of biochemistry

authors： Kametani F,Takayasu T,Suzuki S,Shinoda T,Okuyama T,Shimizu A

更新日期：1983-02-01 00:00:00

abstract：:Fibronectin contains at least two distinct oligopeptide sequences serving as signals for the interaction with cell surface adhesion receptors termed integrins. One of these sequences, Arg-Gly-Asp-Ser (RGDS) tetrapeptide, was shown to be transferred to a truncated form of Staphylococcal IgG-binding protein (hereafter r...

journal_title：Journal of biochemistry

authors： Maeda T,Oyama R,Titani K,Sekiguchi K

更新日期：1993-01-01 00:00:00

abstract：:The alkalophile NADH dehydrogenase (NADH: 2,6-dichlorophenolindophenol oxidoreductase) [EC 1.6.99.3] consists of two identical subunits of 65 kDa, and each subunit contains the catalytic and liposome-binding regions. On treatment with trypsin, the polypeptide exhibiting the liposome-binding property in one of the subu...

journal_title：Journal of biochemistry

authors： Xu XM,Kanaya S,Koyama N,Sekiguchi T,Nosoh Y,Ohashi S,Tsuda K

更新日期：1989-04-01 00:00:00

abstract：:We previously isolated a Ca2+-binding protein from a ciliate, Tetrahymena, and designated it as TCBP (Tetrahymena Ca2+-binding protein). The present paper reports that TCBP, which has two high affinity Ca2+-binding sites (Kd=4.6 X 10(-6) M), could activate porcine brain cyclic nucleotide phosphodiesterase at a concent...

journal_title：Journal of biochemistry

authors： Suzuki Y,Nagao S,Abe K,Hirabayashi T,Watanabe Y

更新日期：1981-01-01 00:00:00

abstract：:The aminopeptidase P gene in Escherichia coli HB101 was cloned into the plasmid pBR322. Introduction of the hybrid plasmid, pAPP01, into the E. coli DH1 resulted in an 8-fold increase of aminopeptidase P activity as compared with that of the host. The enzyme was purified by series of chromatographies on DEAE-Sephadex,...

journal_title：Journal of biochemistry

authors： Yoshimoto T,Murayama N,Honda T,Tone H,Tsuru D

更新日期：1988-07-01 00:00:00

abstract：:We examined the inhibitory effect of three pseudo-aminosugars (validamine, valienamine, and valiolamine), which were isolated from the broth of Streptomyces hygroscopicus, on the oligosaccharide-processing glucosidases I and II involved in glycoprotein biosynthesis in rat liver. Both glucosidases I and II were inhibit...

journal_title：Journal of biochemistry

authors： Takeuchi M,Kamata K,Yoshida M,Kameda Y,Matsui K

更新日期：1990-07-01 00:00:00

abstract：:Gp91(phox) is a key component of the phagocyte NADPH oxidase. Mutations of its promoter found in patients with chronic granulomatous disease cause deficient binding of PU.1 and HAF-1. Because the two factors bind to the same site (Pu box) of the promoter, we attempted to clarify their relative in vivo contributions to...

journal_title：Journal of biochemistry

authors： Islam MR,Fan C,Fujii Y,Hao LJ,Suzuki S,Kumatori A,Yang D,Rusvai E,Suzuki N,Kikuchi H,Nakamura M

更新日期：2002-04-01 00:00:00

abstract：:A group B streptococcus, Streptococcus agalactiae type Ib, produces a high-molecular-weight polysaccharide consisting of the following pentasaccharide repeating unit: -->4)-[alpha-D-NeupNAc-(2-->3)-beta-D-Galp-(1-->3)-beta-D-GlcpNAc-(1-->3)]-beta-D-Galp-(1-->4)-beta-D-Glcp-(1-->. The type-specific capsular polysacchar...

journal_title：Journal of biochemistry

authors： Watanabe M,Miyake K,Yanae K,Kataoka Y,Koizumi S,Endo T,Ozaki A,Iijima S

更新日期：2002-02-01 00:00:00

abstract：:We sought to evaluate the mechanism(s) associated with pro matrix metalloprotease 2 (proMMP-2) activation in bovine pulmonary artery smooth muscle cells. Preincubation of cells with anti-TNFR1 antibody prevented tumour necrosis factor-α (TNF-α)-induced proMMP-2 activation and increase in membrane type 1 matrix metallo...

journal_title：Journal of biochemistry

authors： Roy S,Chakraborti T,Chowdhury A,Chakraborti S

更新日期：2013-03-01 00:00:00