Sliding and ATPase.

abstract：:The crystal structure of a ternary complex of meso-2,3-butanediol dehydrogenase with NAD+ and a competitive inhibitor, mercaptoethanol, has been determined at 1.7 A resolution by means of molecular replacement and refined to a final R-factor of 0.194. The overall structure is similar to those of the other short chain ...

journal_title：Journal of biochemistry

authors： Otagiri M,Kurisu G,Ui S,Takusagawa Y,Ohkuma M,Kudo T,Kusunoki M

更新日期：2001-02-01 00:00:00

abstract：:beta1,4-galactosyltransferase II (beta1,4GalT II) is one of the enzymes transferring galactose to the terminal N-acetylglucosamine of complex-type N-glycans. Previously, we have reported that beta1,4GalT II overexpression increased cisplatin-induced HeLa cell apoptosis. However, the mechanisms of its expression regula...

journal_title：Journal of biochemistry

authors： Zhou J,Wei Y,Liu D,Ge X,Zhou F,Jiang XY,Gu J

更新日期：2008-04-01 00:00:00

abstract：:We obtained two kinds of vesicle preparations which were of different density from the same gastric mucosae of hogs stimulated with food before slaughter. Both kinds contained H+,K+-ATPase. The light vesicle preparation differed from the heavy vesicle preparation as follows: the KCl permeability across the membrane of...

journal_title：Journal of biochemistry

authors： Asano S,Iino T,Tabuchi Y,Takeguchi N

更新日期：1988-04-01 00:00:00

abstract：:The yakC gene in Schizosaccharomyces pombe, which encodes yakC protein (YakC), a potential member of an aldo-keto reductase (AKR) family, was cloned and expressed in Escherichia coli cells. The recombinant YakC purified to homogeneity catalyzed the reduction of 2-nitrobenzaldehyde (k(cat), 44.1 s(-1), K(m), 0.185 +/- ...

journal_title：Journal of biochemistry

authors： Morita T,Huruta T,Ashiuchi M,Yagi T

更新日期：2002-10-01 00:00:00

abstract：:Covalently closed-circular, superhelical DNAs, including viral DNAs, bacterial plasmid DNAs, and bacteriophage replicative-form DNA, were treated with a small amount of Haemophilus gallinarum DNA-relaxing enzyme to generate incompletely relaxed DNA molecules. Each sample consisted of a set of closed-circular DNA molec...

journal_title：Journal of biochemistry

authors： Shishido K

更新日期：1979-09-01 00:00:00

abstract：:The interactions of substoichiometric TNP-ATP and F1-ATPase from Escherichia coli (EF1) were examined and compared with those in the case of mitochondrial F1-ATPase (MF1) and F1-ATPase from thermophilic Bacillus PS3 (TF1). EF1 hydrolyzed substoichiometric TNP-ATP faster than TF1 or MF1, although some 20% of the TNP-AT...

journal_title：Journal of biochemistry

authors： Muneyuki E,Hisabori T,Sasayama T,Mochizuki K,Yoshida M

更新日期：1996-11-01 00:00:00

abstract：:The kinetics of the hydrogen-deuterium exchange reaction in sarcoplasmic reticulum (SR) membranes isolated from rabbit skeletal muscle was followed by infrared absorption measurements. The exchange rate in SR was much lower than that in the soluble proteins reported so far. When adenylyl-imidophosphate (AMPPNP, an TP ...

journal_title：Journal of biochemistry

authors： Anzai K,Kirino Y,Shimizu H

更新日期：1981-08-01 00:00:00

abstract：:Mammalian/mechanistic target of rapamycin (mTOR) is a highly conserved serine/threonine kinase that responds to environmental determinants such as growth factor concentration, nutrient availability, energy sufficiency and stress. mTOR forms two different complexes, called mTOR complex 1 (mTORC1) and mTORC2, and these ...

journal_title：Journal of biochemistry

authors： Hoshii T,Matsuda S,Hirao A

更新日期：2014-08-01 00:00:00

abstract：:An open reading frame of 828 base pairs was found in the CHO1 gene region of Saccharomyces cerevisiae by nucleotide sequencing analysis. Its enhanced expression with the aid of the PHO5 regulatory sequence resulted in an overproduction of a protein with a molecular weight of approximately 30,000, which in turn was con...

journal_title：Journal of biochemistry

authors： Kiyono K,Miura K,Kushima Y,Hikiji T,Fukushima M,Shibuya I,Ohta A

更新日期：1987-11-01 00:00:00

abstract：:The most reactive single thiol group of rabbit skeletal muscle phosphofructokinase per protomer was modified with the following thiol reagents: iodoacetamide, iodoacetate, 2-hydroxyethyl disulfide, 3,3'-dithiodipropionate, and glutathione disulfide. As a result of the modification, there was increase in not only the a...

journal_title：Journal of biochemistry

authors： Nakajima Y,Nakamura K

更新日期：1991-02-01 00:00:00

abstract：:The specificity of highly purified rat liver cathepsin D was investigated by analyzing the digests of denatured proteins. At the P1 site, cathepsin D prefers hydrophobic residues except Ile and Val, that are branched at the beta-carbon. Strong and weak hydrophobicities are required at P1' and P2 sites, respectively. A...

journal_title：Journal of biochemistry

authors： Imoto T,Okazaki K,Koga H,Yamada H

更新日期：1987-03-01 00:00:00

abstract：:The epitopes of about 100 monoclonal antibodies against human type II DNA topoisomerase were mapped along the enzyme molecules. Although they were randomly and independently established, epitope sites were unevenly distributed the toward N-terminal or C-terminal region. We suggest that the central catalytic domain is ...

journal_title：Journal of biochemistry

authors： Sakaguchi A,Miyaike M,Kuroda K,Nozaki N,Tanaka M,Hibino M,Fujii Y,Kato S,Kikuchi A

更新日期：2002-09-01 00:00:00

abstract：:Mannan, a ligand for the mannose/N-acetylglucosamine (GlcNAc) receptor, induces suppression of oxygen consumption and increases glucose production in the perfused rat liver, and repeated infusion of mannan causes desensitization of the responses. In this study, we examined whether activation of Kupffer cells by endoto...

journal_title：Journal of biochemistry

authors： Kimura K,Hamada M,Moriyama M,Kannan Y,Shiota M,Sakurada K,Musashi M,Sugano T

更新日期：1996-09-01 00:00:00

abstract：:The steric course of the enoyl reduction catalyzed by fatty acid synthetase was investigated with the enzymes from bakers' yeast, rat liver and Brevibacterium ammoniagenes. The non-enzymic hydrogen-deuterium exchange of the methylene group of malonyl-CoA was studied by NMR spectroscopy. The half-life period of the met...

journal_title：Journal of biochemistry

authors： Saito K,Kawaguchi A,Seyama Y,Yamakawa T,Okuda S

更新日期：1981-12-01 00:00:00

abstract：:We isolated three genomic clones of rat P-450(11 beta) genes (CYP11B). Two of them corresponded to 11 beta-hydroxylase gene (CYP11B1) and aldosterone synthase gene (CYP11B2), respectively. The third one was a novel gene resembling both CYP11B1 and CYP11B2, and was named CYP11B3 gene (CYP11B3). CYP11B2 and CYP11B3 are ...

journal_title：Journal of biochemistry

authors： Nomura M,Morohashi K,Kirita S,Nonaka Y,Okamoto M,Nawata H,Omura T

更新日期：1993-02-01 00:00:00

abstract：:Corynebacterium diphtheriae, a pathogenic Gram-positive bacterium, contains sialic acids on its cell surface, but no genes related to sialic acid decoration or metabolism have been reported in C. diphtheriae. In the present study, we have identified a putative sialidase gene, nanH, from C. diphtheriae KCTC3075 and cha...

journal_title：Journal of biochemistry

authors： Kim S,Oh DB,Kwon O,Kang HA

更新日期：2010-04-01 00:00:00

abstract：:Upregulation and nuclear retention of the human histone demethylase LSD1 are correlated with aggressiveness and poor outcome of several cancer types, but the molecular mechanism of LSD1 nuclear import remains unclear. Here, we found that the N-terminal flexible region of LSD1 contains a nuclear localization signal (NL...

journal_title：Journal of biochemistry

authors： Jin Y,Kim TY,Kim MS,Kim MA,Park SH,Jang YK

更新日期：2014-12-01 00:00:00

abstract：:The STPR domain is a novel DNA-binding domain composed of repeats of 23 amino-acid-long peptide found in the fibroin-modulator-binding protein-1 (FMBP-1) of the silkworm Bombyx mori. Theoretical proteins having the STPR domain are highly conserved, particularly in vertebrates, but the functions are mostly unknown. In ...

journal_title：Journal of biochemistry

authors： Takiya S,Saito S,Yokoyama T,Matsumoto D,Aizawa T,Kamiya M,Demura M,Kawano K

更新日期：2009-07-01 00:00:00

abstract：:Nearly full-length cDNA clones for muscle-type and non-muscle-type aldolase mRNAs were cloned from lambda gt10 cDNA libraries constructed from skeletal muscle and liver mRNAs of lamprey (Entosphenus japonicus). The cDNA-M8 has 2,240 bp carrying an open reading frame of 1,089 bp which encodes 362 amino acids without th...

journal_title：Journal of biochemistry

authors： Zhang R,Yatsuki H,Kusakabe T,Iwabe N,Miyata T,Imai T,Yoshida M,Hori K

更新日期：1995-03-01 00:00:00

abstract：:The amino acid sequences of human histones have been investigated for studies of histone evolution. The whole histone was prepared from human spleen and was separated into 3 fractions, H4+H3+H2A, H2B, and H1, by our technique of CM-cellulose chromatography. The H2B fraction was further purified by Bio-Gel P-60 chromat...

journal_title：Journal of biochemistry

authors： Ohe Y,Hayashi H,Iwai K

更新日期：1979-02-01 00:00:00

abstract：:A simple and rapid purification method for human serum C-reactive protein (CRP) was developed. CRP was strongly adsorbed on a DEAE-cellulose column and was easily separated from other serum proteins. CRP was purified approximately 1,000-fold with a high yield (50%). The final preparation showed a single band as judged...

journal_title：Journal of biochemistry

authors： Tsujimoto M,Inoue K,Nojima S

更新日期：1983-11-01 00:00:00

abstract：:We isolated and characterized an anti-idiotype monoclonal antibody (AR42.1) which is capable of mimicking a distinct and specific epitope of MUC-1 antigen. The cDNA sequences coding for the AR42.1 variable regions were determined. We found significant amino acid homology between complementary determining regions 3 (CD...

journal_title：Journal of biochemistry

authors： Luo D,Qi W,Ma J,Wang YJ,Wishart D

更新日期：2000-09-01 00:00:00

abstract：:Manganese ion, like Mg2+, has been found to produce high biosynthetic activity of the unadenylylated form of glutamine synthetase obtained from Mycobacterium smegmatis, and the activity with each of these cations was decreased by the adenylylation of the enzyme. Further, the gamma-glutamyltransferase reaction was cata...

journal_title：Journal of biochemistry

authors： Matsuoka K,Kimura K

更新日期：1985-04-01 00:00:00

abstract：:The circadian change of gluconeogenesis in the liver in vivo in fed rats was studied. Gluconeogenesis in the liver in vivo was determined by measuring the rate of synthesis of 14C-labeled blood glucose derived from a 14C-labeled substrate, such as lactate, pyruvate or an amino acid injected into nephrectomized rats. G...

journal_title：Journal of biochemistry

authors： Kida K,Nishio T,Yokozawa T,Nagai K,Matsuda H,Nakagawa H

更新日期：1980-10-01 00:00:00

abstract：:Sporulation of Bacillus subtilis, growing exponentially in the presence of rapidly metabolizable nutrients, was induced by addition of decoyinine (an antibiotic inhibitor of GMP synthesis), and intracellular amounts of ppGpp were determined after 2 M formic acid extraction by polyethyleneimine (PEI)-cellulose thin-lay...

journal_title：Journal of biochemistry

authors： Ikehara K,Okamoto M,Sugae K

更新日期：1982-03-01 00:00:00

abstract：:It was previously found that rabbit serum contains a growth-inhibitory substance for a tumorigenic rat liver cell line RSV-BRL. In the present study, the growth inhibitor was purified from normal rabbit serum to show a homogeneous protein band with a molecular weight (Mr) of 56 k on SDS-polyacrylamide gel electrophore...

journal_title：Journal of biochemistry

authors： Kimura T,Miyazaki K,Mashima K,Yamashita J,Horio T,Kakuno T

更新日期：1991-09-01 00:00:00

abstract：:Paranitrophenylphosphate (pNPP) induced fluorescence changes in fluorescence isothiocyanate (FITC)-labeled Na+,K(+)-ATPase preparations. The extents of changes were similar to those induced by acetylphosphate (AcP) accompanying accumulation of a K(+)-sensitive phosphoenzyme (E2P) and an ouabain bound phosphoenzyme in ...

journal_title：Journal of biochemistry

authors： Yamazaki A,Kaya S,Tsuda T,Araki Y,Hayashi Y,Taniguchi K

更新日期：1994-12-01 00:00:00

abstract：:Using the methods of methylation/GLC, MS, and 1H-NMR analyses, a disaccharide structure of GalNAc alpha 1----2Fuc1---- was identified as one of the major structural units at non-reducing terminals of a highly branched and N-glycosidically linked carbohydrate on an allergenically active glycopeptide, Gp-2 (Mr ca. 8,000...

journal_title：Journal of biochemistry

authors： Shigeta S,Ono K,Ohta M,Kobayashi Y,Oka S

更新日期：1989-05-01 00:00:00

abstract：:We can detect phospholipase A activity in non-hemolyzed sheep erythrocytes, using dilauroylglycerophosphocholine as an exogenous substrate. Only substrates such as dilauroylglycerophosphocholine, which can be incorporated into membranes, could be hydrolyzed by the enzyme, egg phosphatidylcholine being only slightly se...

journal_title：Journal of biochemistry

authors： Tanaka Y,Eriomin VA,Kawamura Y,Inoue K,Nojima S

更新日期：1983-09-01 00:00:00

abstract：:Kinetic studies of the reduction of plastocyanin from spinach chloroplasts by ascorbate were carried out using a stopped flow apparatus. The reduction of spinach plastocyanin by ascorbate followed first-order kinetics in the reductant with a rate constant k = 415 M-1.S-1 [20 degrees C, 0.1 M NaCl, pH 8.0 (10 mM Tris-H...

journal_title：Journal of biochemistry

authors： Takabe T,Niwa S,Ishikawa H,Miyakawa M

更新日期：1980-01-01 00:00:00