Properties of light and heavy vesicles simultaneously prepared from hog gastric mucosae.


:We obtained two kinds of vesicle preparations which were of different density from the same gastric mucosae of hogs stimulated with food before slaughter. Both kinds contained H+,K+-ATPase. The light vesicle preparation differed from the heavy vesicle preparation as follows: the KCl permeability across the membrane of heavy vesicles was larger than that of light vesicles, the actin (46-kDa peptide on SDS-polyacrylamide gel) content of heavy vesicles was much higher than that of light vesicles, and the H+,K+-ATPase activity of heavy vesicles was less sensitive to a monoclonal antibody raised against light vesicles (HK2032) than that of light vesicles. Furthermore, there was a drastic difference in reactivity to SCH 28080, which is an H+,K+-ATPase-specific inhibitor and reacts competitively with the K+-high affinity site. SCH 28080 is more potent in light vesicles than in heavy vesicles. These results suggest that the conformation of H+,K+-ATPase changed during the translocation from tubulovesicles to the apical plasma membrane. On the other hand, H+,K+-ATPase activities in both vesicles had similar pH and [K+] dependences.


J Biochem


Journal of biochemistry


Asano S,Iino T,Tabuchi Y,Takeguchi N




Has Abstract


1988-04-01 00:00:00












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    abstract::Nitric oxide (NO) has diverse effects on immune responses and hepatic functions. In BNL CL.2 cells, the murine embryonic liver cells, inducible nitric oxide synthase (iNOS) mRNA expression appeared after 3 h of treatment with IFN-gamma and LPS. Interestingly, mRNA and protein expression of iNOS was down-regulated by s...

    journal_title:Journal of biochemistry

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    authors: Lee BS,Kim YM,Kang HS,Kim HM,Pyun KH,Choi I

    更新日期:2001-01-01 00:00:00

  • Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein.

    abstract::Hepatitis C virus (HCV) core protein has been suggested to play crucial roles in the pathogeneses of liver steatosis and hepatocellular carcinomas due to HCV infection. Intracellular HCV core protein is localized mainly in lipid droplets, in which the core protein should exert its significant biological/pathological f...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sato S,Fukasawa M,Yamakawa Y,Natsume T,Suzuki T,Shoji I,Aizaki H,Miyamura T,Nishijima M

    更新日期:2006-05-01 00:00:00

  • Ionic strength and pH effects on the rates of reduction of spinach plastocyanin by ascorbate.

    abstract::Kinetic studies of the reduction of plastocyanin from spinach chloroplasts by ascorbate were carried out using a stopped flow apparatus. The reduction of spinach plastocyanin by ascorbate followed first-order kinetics in the reductant with a rate constant k = 415 M-1.S-1 [20 degrees C, 0.1 M NaCl, pH 8.0 (10 mM Tris-H...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Takabe T,Niwa S,Ishikawa H,Miyakawa M

    更新日期:1980-01-01 00:00:00

  • Chemical modifications of ribonuclease U1.

    abstract::In order to obtain information on the nature of the amino acid residues involved in the activity of ribonuclease U1 [EC], various chemical modifications of the enzyme were carried out. RNase U1 was inactivated by reaction with iodoacetate at pH 5.5 with concomitant incorporation of 1 carboxymethyl group per mo...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hashimoto J,Takahashi K

    更新日期:1977-04-01 00:00:00

  • Identification of the roles of individual amino acid residues of the helix E of the major antenna of photosystem II (LHCII) by alanine scanning mutagenesis.

    abstract::The functions of the helix E (W97-F105), an amphiphilic lumenal 310 helix of the major antenna of photosystem II (LHCII), are still unidentified. To elucidate the roles of individual amino acid residue of the helix E, alanine scanning mutagenesis has been performed to mutate every residue of this domain to alanine. Th...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Liu C,Rao Y,Zhang L,Yang C

    更新日期:2014-10-01 00:00:00

  • A comparative study of high molecular weight urokinase and low molecular weight urokinase.

    abstract::Two forms of urokinase [EC] with molecular weights of 51,600 and 34,500 were purified from human urine. The specific activities of the high molecular weight urokinase (HMW-UK) and low molecular weight urokinase (LMW-UK) were 157,400 and 246,700 International Units (IU/mg), respectively. Purified HMW-UK was 9...

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    pub_type: 杂志文章


    authors: Nobuhara M,Sakamaki M,Ohnishi H,Suzuki Y

    更新日期:1981-07-01 00:00:00

  • Surface potential dependence of the distribution of charged dye molecules onto photosynthetic membranes.

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Masamoto K,Matsuura K,Itoh S,Nishimura M

    更新日期:1981-02-01 00:00:00

  • Chemical modification of the most reactive thiol group of rabbit skeletal muscle phosphofructokinase, to reduce its affinity toward substrate ATP and activating monovalent cations.

    abstract::The most reactive single thiol group of rabbit skeletal muscle phosphofructokinase per protomer was modified with the following thiol reagents: iodoacetamide, iodoacetate, 2-hydroxyethyl disulfide, 3,3'-dithiodipropionate, and glutathione disulfide. As a result of the modification, there was increase in not only the a...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nakajima Y,Nakamura K

    更新日期:1991-02-01 00:00:00

  • Isolation of a membrane protein from R rubrum chromatophores and its abnormal behavior in SDS-polyacrylamide gel electrophoresis due to a high binding capacity for SDS.

    abstract::A membrane protein insoluble in water was isolated by gel chromatography in the presence of 0.1% sodium dodecyl sulfate (SDS) from chromatophores of a photosynthetic bacterium, Rhodospirillum rubrum. This is one of the major membrane proteins of the chromatophore. The protein was found to bind about four grams of SDS ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Miyake J,Ochiai-Yanagi S,Kasumi T,Takagi T

    更新日期:1978-06-01 00:00:00

  • Modification of glucoamylases from Rhizopus sp. with 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl)carbodiimide.

    abstract::To investigate the role of carboxyl groups of glucoamylases [EC] from a Rhizopus sp. (Gluc1 and Gluc2), the modification of Gluc1 and Gluc2 with a water-soluble carbodiimide, 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl)carbodiimide metho-p-toluenesulfonate (CMC), was studied. The inactivation of Gluc1 proceeded wi...

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    pub_type: 杂志文章


    authors: Iwama M,Ohtsuki R,Takahashi T,Irie M

    更新日期:1984-08-01 00:00:00

  • Amino acid sequence of glutathione S-transferase a from guinea pig liver.

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kamei-Hayashi K,Oshino R,Hara S

    更新日期:1993-12-01 00:00:00

  • Mitochondrial targeting signal-induced conformational change and repression of the peroxisomal targeting signal of the precursor for rat liver serine:pyruvate/alanine:glyoxylate aminotransferase.

    abstract::In the rat liver, two mRNAs for serine:pyruvate (or alanine:glyoxylate) aminotransferase are generated from a single gene by alternative transcription initiation. The longer mRNA encodes a precursor of a mitochondrial enzyme that has a mitochondrial targeting signal at the N-terminus and is translocated into mitochond...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Oda T,Uchida C,Miura S

    更新日期:2000-04-01 00:00:00

  • Isolation of N omega-phosphoarginine hydrolase from rat liver and its physical properties.

    abstract::N omega-Phosphoarginine hydrolase from rat liver cytosol was purified to apparent homogeneity on SDS-PAGE, by employing column chromatographies on Sephadex G-75, DEAE-cellulose, QAE-Toyopearl, and glutathione-2-pyridyl-disulfide-Superose. One milligram protein of the final preparation released 4 mumol/min of inorganic...

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    pub_type: 杂志文章


    authors: Yokoyama K,Ohmori H,Kumon A

    更新日期:1993-02-01 00:00:00

  • Evidence for functional involvement of asparagine 67 in substrate recognition by snake venom phospholipases A2.

    abstract::Site-directed mutagenesis studies of recombinant Trimeresurus flavoviridis venom gland phospholipase A2 (PLA2) showed that the Asn residue at position 67 takes part in recognition of the substrate 2-arachidonoyl sn-glycero-3-phosphocholine in both monomeric and micellar states. The amount of arachidonate released from...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


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    更新日期:1997-11-01 00:00:00

  • Molecular cloning and in situ localization in the brain of rat sky receptor tyrosine kinase.

    abstract::We previously identified a human cDNA encoding a novel receptor tyrosine kinase, termed Sky, which is predominantly expressed in the brain and has a unique extracellular domain consisting of two immunoglobulin (Ig)-like and two fibronectin type III (FN III) motifs. In attempts to define the functional role of the Sky ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ohashi K,Honda S,Ichinomiya N,Nakamura T,Mizuno K

    更新日期:1995-06-01 00:00:00

  • Purification and characterization of methioninase from Pseudomonas putida.

    abstract::Methioninase of Pseudomonas putida was purified to homogeneity, as judged by polyacrylamide gel electrophoresis, with a specific activity 270-fold higher than that of the crude extract. 1. The purified enzyme had an S20,w of 8.37, a molecular weight of 160,000, and an isoelectric point of 5.6. 2. A break in the Arrhen...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ito S,Nakamura T,Eguchi Y

    更新日期:1976-06-01 00:00:00

  • N- and C-terminal fragments of a globular protein constructed by elongation of modules as a units associated for functional complementation.

    abstract::We have been interested in partially folded proteins with marginal stability and activity, because they have a potential to be mature proteins by artificial evolution. A module is defined as a contiguous peptide chain forming a compact region in a globular protein. Modules may be used as building blocks to create part...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tsuji T,Nagata T,Yanagawa H

    更新日期:2008-10-01 00:00:00

  • Molecular cloning and sequencing of a cDNA encoding alanine-glyoxylate aminotransferase 2 from rat kidney.

    abstract::Alanine-glyoxylate aminotransferase (AGT) 2 is a pyridoxal 5'-phosphate dependent, mitochondrial enzyme which, in the rat, is expressed at a high level in the kidney. The amino acid sequences of nine tryptic and seven CNBr peptides of the rat kidney AGT2 were determined. Three overlapping cDNAs encoding the AGT2 were ...

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    pub_type: 杂志文章


    authors: Lee IS,Muragaki Y,Ideguchi T,Hase T,Tsuji M,Ooshima A,Okuno E,Kido R

    更新日期:1995-04-01 00:00:00

  • Nucleotide-binding sites in V-type Na+-ATPase from Enterococcus hirae.

    abstract::Enterococcus hirae V-ATPase, in contrast to most V-type ATPases, is resistant to N-ethylmaleimide (NEM). Alignment of the amino acid sequences of NtpA suggests that the NEM-sensitive Cys of V-type ATPases is replaced by Ala in E. hirae V-ATPase. Consistent with this prediction, the V-ATPase became sensitive upon subst...

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    pub_type: 杂志文章


    authors: Murata T,Yoshikawa Y,Hosaka T,Takase K,Kakinuma Y,Yamato I,Kikuchi T

    更新日期:2002-11-01 00:00:00

  • Elevation of plasma membrane permeability on laser irradiation of extracellular latex particles.

    abstract::In this report, we describe a laser-latex combination system that enables membrane-impermeable molecules to penetrate cell membranes. Laser light (Q-switched Nd:YAG laser, 532.5 nm) was used to irradiate a mixture of commercial latex particles (blue dyed, 1 micro m in diameter) and mouse fibrosarcoma (Meth-A) cells. A...

    journal_title:Journal of biochemistry

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    authors: Umebayashi Y,Miyamoto Y,Wakita M,Kobayashi A,Nishisaka T

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  • Peptide bond synthesis catalyzed by subtilisin, papain, and pepsin.

    abstract::The peptide bond syntheses catalyzed by subtilisin BPN', papain [EC], and pepsin [EC] were studied comparatively at the optimum pH of the enzymes with the coupling system Cbz-(AA)n-OH + Leu-X leads to Cbz-(AA)n-Leu-X, in which AA = various amino acid residues, n = 1-3 and X = -NH2, -OEt, -OBut, -ODPM...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Morihara K,Oka T

    更新日期:1981-02-01 00:00:00

  • Interaction of mitochondrial aspartate aminotransferase with negatively charged lecithin liposomes.

    abstract::Several kinds of hydrophilic proteins were examined to determine their interaction with artificial liposomes. Mitochondrial aspartate aminotransferase (m-GOT) [EC], as well as cytochrome c, was found to interact strongly with negatively charged liposomes. In each case, an appreciable amount of the protein boun...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Furuya E,Yoshida Y,Tagawa K

    更新日期:1979-05-01 00:00:00

  • Purification of the most abundant protein in the coelomic fluid of a sea urchin which immunologically cross reacts with 23S glycoprotein in the sea urchin eggs.

    abstract::The most abundant glycoprotein in the coelomic fluid of sea urchin Anthocidaris crassispina was purified and its subunit structure, molecular form in the native state, amino acid composition, and electron micrographic image were studied. The results showed that the protein in its native state was basically a tetramer ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Giga Y,Ikai A

    更新日期:1985-07-01 00:00:00

  • Tyrosine-specific dephosphorylation-phosphorylation with alkaline phosphatases and epidermal growth factor receptor kinase as evidenced by 31P NMR spectroscopy.

    abstract::The dephosphorylation of phospho-amino acids with alkaline phosphatase (AlPase) from calf intestine or Escherichia coli and the phosphorylation of bovine serum albumin (BSA) with epidermal growth factor (EGF) receptor kinase from human A431 epidermoid carcinoma cells were investigated by 31P NMR spectroscopy. The init...

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    更新日期:1987-05-01 00:00:00

  • Deletion analysis of protein kinase Calpha reveals a novel regulatory segment.

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Rotenberg SA,Zhu J,Hansen H,Li XD,Sun XG,Michels CA,Riedel H

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  • A new Tetrahymena actin-binding protein is localized in the division furrow.

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


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    更新日期:1998-04-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Mabuchi I

    更新日期:1983-10-01 00:00:00

  • Studies on glycosphingolipids of fresh-water bivalves. VI. Isolation and chemical characterization of neutral glycosphingolipids from spermatozoa of the fresh-water bivalve, Hyriopsis schlegelii.

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sugita M,Yamamoto T,Masuda S,Itasaka O,Hori T

    更新日期:1981-11-01 00:00:00

  • Expression and imprinting status of human PEG8/IGF2AS, a paternally expressed antisense transcript from the IGF2 locus, in Wilms' tumors.

    abstract::A large imprinted gene cluster in human chromosome 11p15.5 has been implicated in Beckwith-Wiedemann syndrome and Wilms' tumor. We have identified a paternally expressed imprinted gene, PEG8/IGF2AS, in this locus. It is transcribed in the opposite direction to the IGF2 transcripts and some genomic regions are shared w...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Okutsu T,Kuroiwa Y,Kagitani F,Kai M,Aisaka K,Tsutsumi O,Kaneko Y,Yokomori K,Surani MA,Kohda T,Kaneko-Ishino T,Ishino F

    更新日期:2000-03-01 00:00:00

  • Sex-related difference in vitamin D3 25-hydroxylase of rat liver microsomes.

    abstract::Cholecalciferol 25-hydroxylase was partially purified by polyethylene glycol fractionation and chromatographies on octylamino-Sepharose and hydroxylapatite columns starting from the liver microsomes of female rats, and compared with P-450cc25 purified from the liver microsomes of male rats (Hayashi, et al. (1986) J. B...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hayashi S,Usui E,Okuda K

    更新日期:1988-05-01 00:00:00