Structural and functional characterization of haemocyanin from the anemone hermit crab Dardanus calidus.

abstract：:A radioimmunoassay for chicken calcitonin in chicken ultimobranchial glands was established utilizing a rabbit antiserum against eel calcitonin. This assay method, which is about 100 times as sensitive as the usual bioassay for hypocalcemic activity, was used for monitoring chicken calcitonin during its purification. ...

journal_title：Journal of biochemistry

authors： Homma T,Watanabe M,Hirose S,Kanai A,Kangawa K,Matsuo H

更新日期：1986-08-01 00:00:00

abstract：:The ATPase inhibitor from yeast, Saccharomyces cerevisiae, was found to strongly inhibit the ATPase activity when it was preincubated in an acidic pH region. This paper describes the conformational changes of the inhibitor in the acidic pH region, as studied by 1H NMR spectroscopy. In the pH range from 7.43 to 4.48, t...

journal_title：Journal of biochemistry

authors： Fujii S,Hashimoto T,Yoshida Y,Miura R,Yamano T,Tagawa K

更新日期：1983-01-01 00:00:00

abstract：:Autotaxin (ATX) is an ecto-enzyme responsible for lysophosphatidic acid (LPA) production in blood. ATX is present in various biological fluids such as cerebrospinal and seminal fluids and accounts for bulk LPA production in these fluids. ATX is a member of the nucleotide pyrophosphatase/phosphodiesterase (NPP) family ...

journal_title：Journal of biochemistry

authors： Nakanaga K,Hama K,Aoki J

更新日期：2010-07-01 00:00:00

abstract：:Site-specific recognition and DNA-binding activity of p53 are crucial for its tumour suppressor function. Previous reports have shown that metal ions can affect the specific recognition and DNA-binding activity of p53DBD. Here we firstly report that magnesium ion can bind to the protein and influence its DNA-binding a...

journal_title：Journal of biochemistry

authors： Xue Y,Wang S,Feng X

更新日期：2009-07-01 00:00:00

abstract：:We designed a simple procedure for the purification of peptidylarginine deiminase, which catalyzes the deimination of arginyl residues in protein, from rabbit skeletal muscle using substrate affinity chromatography. Of the immobilized substrate ligands tested, i.e. protamine and soybean trypsin inhibitor (Kunitz) (STI...

journal_title：Journal of biochemistry

authors： Takahara H,Okamoto H,Sugawara K

更新日期：1986-05-01 00:00:00

abstract：:The purpose of this study was to determine whether ATP-sensitive potassium channel (K(ATP) channel) activation generates oxygen free radicals in the rabbit heart. We assayed malondialdehyde (MDA) in rabbit heart slices in vitro as an indicator of oxygen free radical generation. The K(ATP) channel openers, pinacidil an...

journal_title：Journal of biochemistry

authors： Han J,Kim N,Park J,Seog DH,Joo H,Kim E

更新日期：2002-05-01 00:00:00

abstract：:Ornithine transcarbamylase (OTC) is one of the urea cycle enzymes. While the chicken is a uricotelic animal, it has measurable OTC activity in its kidney. OTC activity is highly variable within and between chicken breeds. Chicken OTC may have some physiological significance because of its significant activity in the k...

journal_title：Journal of biochemistry

authors： Shimogiri T,Kono M,Mannen H,Mizutani M,Tsuji S

更新日期：1998-11-01 00:00:00

abstract：:The present study demonstrates that the functional counter-receptors for E-selectin at the cell surface of Colo201 human colon cancer cells are localized in detergent-insoluble membrane microdomains (DIM). Following isolation of counter-receptors from whole cell lysates using E-selectin-coupled magnetic beads followed...

journal_title：Journal of biochemistry

authors： Suzuki C,Kojima N

更新日期：2007-07-01 00:00:00

abstract：:We recently described the cloning of putative human CMP-sialic acid transporter (hCST) cDNA [Ishida, N. et al. (1996) J. Biochem. 120, 1074-1078]. The hCST cDNA coded for a hydrophobic protein with an amino acid sequence showing a high degree of similarity (92% identity) to that of murine CMP-sialic acid transporter. ...

journal_title：Journal of biochemistry

authors： Ishida N,Ito M,Yoshioka S,Sun-Wada GH,Kawakita M

更新日期：1998-07-01 00:00:00

abstract：:Actin-activated ATPase activity of myosin from Physarum polycephalum decreases when it binds Ca2+ and increases when it loses Ca2+. This Ca-inhibition is observed with phosphorylated myosin [Kohama, K. (1990) Trend, Pharmacol. Sci. 11, 433-435]. The activity of dephosphorylated myosin remained at a low level both in t...

journal_title：Journal of biochemistry

authors： Kohama K,Kohno T,Okagaki T,Shimmen T

更新日期：1991-10-01 00:00:00

abstract：:Genomic clones encoding the Drosophila aldolase gene were isolated and the organization of the gene was determined. The protein-coding region spanning nearly 3.5 kb consists of five coding exons (exon 2, 3, 4 alpha, 4 beta, and 4 gamma). The insect exon 2 corresponds to exons 2 to 7 of vertebrate aldolase genes and th...

journal_title：Journal of biochemistry

authors： Kai T,Sugimoto Y,Kusakabe T,Zhang R,Koga K,Hori K

更新日期：1992-11-01 00:00:00

abstract：:Chorismate mutase of Brevibacterium flavum, a common enzyme in phenylalanine and tyrosine biosynthesis, was separted into two different component, A and B, with molecular weights of 250,000 and 25,000, respectively, by ammonium sulfate fractionation or gel-filtration. Both components were essential for the enzymatic a...

journal_title：Journal of biochemistry

authors： Shiio I,Sugimoto S

更新日期：1979-07-01 00:00:00

abstract：:Paramyosin is a myosin-binding protein characteristic of invertebrate animals, while troponin is a Ca2+-dependent regulator of muscle contraction. Both proteins are widely distributed in protostomes, while in deuterostomes, their distribution is limited; namely, presence of paramyosin and absence of troponin are commo...

journal_title：Journal of biochemistry

authors： Sonobe H,Obinata T,Minokawa T,Haruta T,Kawamura Y,Wakatsuki S,Sato N

更新日期：2016-12-01 00:00:00

abstract：:An acid proteinase different from pepsin was purified from bullfrog (Rana catesbeiana) gastric mucosa by chromatography on hydroxyapatite, Q-Sepharose, Con A-Sepharose 4B, and Mono Q columns. Its molecular weight after purification was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 45 kDa...

journal_title：Journal of biochemistry

authors： Inokuchi T,Kobayashi K,Horiuchi S

更新日期：1994-01-01 00:00:00

abstract：:Ectoenzyme release from rat liver and kidney by phosphatidylinositol (PI)-specific phospholipase C of Bacillus thuringiensis was studied. Alkaline phosphatase and 5'-nucleotidase were released from rat kidney slices to extents of up to 60% and 30%, respectively. Release of alkaline phosphatase was observed at lower am...

journal_title：Journal of biochemistry

authors： Taguchi R,Asahi Y,Ikezawa H

更新日期：1985-03-01 00:00:00

abstract：:Spectrophotometric studies revealed the irreversible photodissociation of reduced cytochrome oxidase-nitric oxide (NO) at 5 K. The dissociated NO recombined as the sample temperature was raised, and the half-recombination temperature was 65 K. The photodissociation at 5K was also confirmed by a change in the EPR spect...

journal_title：Journal of biochemistry

authors： Yoshida S,Hori H,Orii Y

更新日期：1980-12-01 00:00:00

abstract：:Transient and steady state kinetics were studied in the interactions of ATP with acto-H-meromyosin reconstituted from bovine arterial heavy-meromyosin (HMM) and rabbit skeletal muscle F-actin. The results showed that the rate of dissociation of the hybrid acto-HMM induced by ATP was slower than the rate of the fluores...

journal_title：Journal of biochemistry

authors： Takeuchi K,Tonomura Y

更新日期：1978-08-01 00:00:00

abstract：:The effect of synthetic polycations, polyallylamine, and polyethylenimine, on liposomes containing phosphatidylserine was investigated along with that of polylysine and divalent cations. The addition of polycations caused aggregation of sonicated vesicles composed of phosphatidylserine and phosphatidylcholine (molar r...

journal_title：Journal of biochemistry

authors： Oku N,Yamaguchi N,Yamaguchi N,Shibamoto S,Ito F,Nango M

更新日期：1986-10-01 00:00:00

abstract：:We expressed human myotonic dystrophy protein kinase (DMPK) in the fission yeast Schizosaccharomyces pombe, in which the overexpression of human DMPK affects cell growth and cell shape. The human DMPK protein has a leucine-rich domain at the N-terminus, a serine/threonine kinase domain in the middle, and a hydrophobic...

journal_title：Journal of biochemistry

authors： Sasagawa N,Kino Y,Takeshita Y,Oma Y,Ishiura S

更新日期：2003-10-01 00:00:00

abstract：:A 5'-N-ethylcarboxamido[3H]adenosine ([3H]NECA) binding site of mouse mastocytoma P815 cell membranes has been purified approximately 100-fold by affinity chromatography. This adenosine binding site, which has a similar specificity to that of the A2 adenosine receptor, was absorbed on NECA-linked Sepharose 6B and elut...

journal_title：Journal of biochemistry

authors： Nakata H

更新日期：1989-05-01 00:00:00

abstract：:The present study was undertaken in an attempt to clarify the pathway by which hyperosmotic stress induces HB-EGF gene expression in rat aortic smooth muscle cells (RASMC). Hyperosmotic stress induced by a high concentration of glucose or mannitol resulted in an increase in HB-EGF mRNA level in a dose- and time-depend...

journal_title：Journal of biochemistry

authors： Koh YH,Che W,Higashiyama S,Takahashi M,Miyamoto Y,Suzuki K,Taniguchi N

更新日期：2001-09-01 00:00:00

abstract：:It was previously found that rabbit serum contains a growth-inhibitory substance for a tumorigenic rat liver cell line RSV-BRL. In the present study, the growth inhibitor was purified from normal rabbit serum to show a homogeneous protein band with a molecular weight (Mr) of 56 k on SDS-polyacrylamide gel electrophore...

journal_title：Journal of biochemistry

authors： Kimura T,Miyazaki K,Mashima K,Yamashita J,Horio T,Kakuno T

更新日期：1991-09-01 00:00:00

abstract：:In this paper we speculate on the origin and early evolution of transition element enzymes. Iron, molybdenum, and zinc, the most abundant transition elements in seawater, presumably complexed with compounds accumulated in the primeval sea in the course of chemical evolution forming compounds with subsequently evolved ...

journal_title：Journal of biochemistry

authors： Egami F

更新日期：1975-06-01 00:00:00

abstract：:Paranitrophenylphosphate (pNPP) induced fluorescence changes in fluorescence isothiocyanate (FITC)-labeled Na+,K(+)-ATPase preparations. The extents of changes were similar to those induced by acetylphosphate (AcP) accompanying accumulation of a K(+)-sensitive phosphoenzyme (E2P) and an ouabain bound phosphoenzyme in ...

journal_title：Journal of biochemistry

authors： Yamazaki A,Kaya S,Tsuda T,Araki Y,Hayashi Y,Taniguchi K

更新日期：1994-12-01 00:00:00

abstract：:Reaction center of chromatophores of Rhodospirillum rubrum consists of three kinds of protein, H-, M-, and L-subunit, and is bound with many other kinds of protein to form a larger protein complex (PRU; photoreaction unit), which contains all the bacteriochlorophyll. In the present study, purified PRU was dissociated ...

journal_title：Journal of biochemistry

authors： Tanaka K,Kakuno T,Yamashita J,Horio T

更新日期：1983-12-01 00:00:00

abstract：:A synchronous enzyme-reaction system using water-soluble formazan and a non-enzymatic electron mediator was developed and applied to an enzyme immunoassay (EIA). The reaction system consists of four steps: (I) dephosphorylation of NADP(+) to produce NAD(+) by alkaline phosphatase (ALP), (II) reduction of NAD(+) to pro...

journal_title：Journal of biochemistry

authors： Inouye K,Ueno I,Yokoyama S,Sakaki T

更新日期：2002-01-01 00:00:00

abstract：:Histidinoalanine, a cross-linking component of connective tissue proteins, was detected in the acid hydrolysate of human urine. The concentrations in urines from newborn babies, children, and adults were 1.33 +/- 0.27, 0.77 +/- 0.23, and 0.89 +/- 0.33 nmol/mg creatinine, respectively. Possible origins of urinary histi...

journal_title：Journal of biochemistry

authors： Fujimoto D

更新日期：1986-09-01 00:00:00

abstract：:Recombinant horse L-apoferritin and its mutants were used to compare the reactivities of two different cysteinyl residues with 7-fluoro-4-sulfamoyl-2,1,3-benzoxadiazole (ABD-F), p-chloromercuribenzoic acid (PCMB), N-(9-acridinyl)maleimide (NAM), and 4-maleimido-2,2,6,6-tetramethylpiperidine-N-oxyl (NEM-TEMPO). ABD-F s...

journal_title：Journal of biochemistry

authors： Takeda S,Yamaki M,Ebina S,Nagayama K

更新日期：1995-02-01 00:00:00

abstract：:The DegS-DegU two-component regulatory system regulates many cellular events in Bacillus subtilis. Genes for DegSU constitutes an operon directed by the P1 promoter and downstream degU is autoregulated via the P3 promoter activated by phosphorylated DegU. In the Gram-positive bacteria, Spx plays a major role in the pr...

journal_title：Journal of biochemistry

authors： Shiwa Y,Yoshikawa H,Tanaka T,Ogura M

更新日期：2015-05-01 00:00:00

abstract：:Rat serum carboxylesterase [carboxylic ester hydrolase, EC 3.1.1.1] was purified by ammonium sulfate precipitation, chromatography on DEAE-cellulose, QAE Sephadex A-50 and brushite, and gel filtration on Sephadex G-200. This purified enzyme was shown to be a single protein band on slab gel electrophoresis and its fina...

journal_title：Journal of biochemistry

authors： Hashinotsume M,Higashino K,Hada T,Yamamura Y

更新日期：1978-12-01 00:00:00