Sex-related difference in vitamin D3 25-hydroxylase of rat liver microsomes.

abstract：:The incorporation of serine and ethanolamine into phospholipids in rabbit retinal subcellular fractions and in excised retinas was studied in vitro, and some enzymic properties of the incorporation of phospholipid bases by base exchange were examined in the microsomal fraction. The retina was found to have a higher ra...

journal_title：Journal of biochemistry

authors： Mizuno A

更新日期：1976-07-01 00:00:00

abstract：:NADP+-specific isocitrate dehydrogenase was found in several tissues of the pupa of the silkworm, Bombyx mori. This enzyme was highly purified from the whole bodies of pupae. This is the first isolation of the enzyme from insect materials. The purified enzyme gave a single protein band on polyacrylamide gel electropho...

journal_title：Journal of biochemistry

authors： Miake F,Torikata T,Koga K,Hayashi K

更新日期：1977-08-01 00:00:00

abstract：:The crystal structures of the GTP- and GDP-bound alpha subunits of heterotrimeric GTP-binding proteins were recently determined, and a conserved Thr residue in the G2 (linker 2) region of the alpha subunits, which corresponds to Thr182 in Gi2 alpha, was deduced to interact with the gamma-phosphate of GTP and Mg2+. To ...

journal_title：Journal of biochemistry

authors： Nishina H,Nimota K,Kukimoto I,Maehama T,Takahashi K,Hoshino S,Kanaho Y,Katada T

更新日期：1995-11-01 00:00:00

abstract：:Ca2+-activated neutral protease (CANP) usually requires mM Ca2+ for activation. The sensitivity of CANP to Ca2+ is greatly enhanced by passing it through a casein-Sepharose column in the presence of Ca2+ ions. This conversion is ascribed to autolysis of CANP. The converted enzyme required 40 microM Ca2+ for 50% activa...

journal_title：Journal of biochemistry

authors： Suzuki K,Tsuji S,Kubota S,Kimura Y,Imahori K

更新日期：1981-07-01 00:00:00

abstract：:We have studied the regio- and stereoselectivity of ring-hydroxylation and N-desisopropylation of S(-)- and R(+)-propranolol, using dog liver microsomes and the expressed dog CYP2D15 in insect cells. In dog liver microsomes, 4-hydroxylation was the preferred pathway in S(-)-propranolol oxidation, while N-desisopropyla...

journal_title：Journal of biochemistry

authors： Tasaki T,Iwata H,Kazusaka A,Fujita S

更新日期：1998-04-01 00:00:00

abstract：:Soybean Bowman-Birk inhibitor, a double-headed inhibitor of trypsin and alpha-chymotrypsin, was treated with cyanogen bromide and then pepsin to yield two inhibitory active fragments. Structural investigation showed that one of the fragments was derived from the trypsin inhibitory domain and the other from the chymotr...

journal_title：Journal of biochemistry

authors： Odani S,Ikenaka T

更新日期：1978-03-01 00:00:00

abstract：:The unfolded protein response is an adaptive stress response that responds to the imbalance between the entry of newly synthesized unfolded proteins and the inherent folding capacity in the endoplasmic reticulum (ER). Various environmental stresses and changes in physiological conditions can result in the accumulation...

journal_title：Journal of biochemistry

authors： Kohno K

更新日期：2010-01-01 00:00:00

abstract：:3-Ketovalidoxylamine A C-N lyase was purified about 900-fold from the cell-free extract of Flavobacterium saccharophilum by ammonium sulfate fractionation, column chromatography on CM cellulose and gel filtration on Sephacryl S-200. The purified enzyme was homogeneous as judged by sodium dodecyl sulfate (SDS) polyacry...

journal_title：Journal of biochemistry

authors： Takeuchi M,Asano N,Kameda Y,Matsui K

更新日期：1985-12-01 00:00:00

abstract：:1. Phospholipase D [EC 3.1.4.4] from Streptomyces hachijoensis was purified about 570-fold by column chromatography on DEAE-cellulose and Sephadex G-50 followed by isoelectric focusing. 2. The purified preparation was found to be homogeneous both by immunodiffusion and polyacrylamide disc gel electrophoresis. 3. The i...

journal_title：Journal of biochemistry

authors： Okawa Y,Yamaguchi T

更新日期：1975-08-01 00:00:00

abstract：:The circadian change of gluconeogenesis in the liver in vivo in fed rats was studied. Gluconeogenesis in the liver in vivo was determined by measuring the rate of synthesis of 14C-labeled blood glucose derived from a 14C-labeled substrate, such as lactate, pyruvate or an amino acid injected into nephrectomized rats. G...

journal_title：Journal of biochemistry

authors： Kida K,Nishio T,Yokozawa T,Nagai K,Matsuda H,Nakagawa H

更新日期：1980-10-01 00:00:00

abstract：:The role of HSDJ, a human homolog of bacterial DnaJ and yeast YDJ1p/MAS5, in mitochondrial protein import was examined. Recombinant HSDJ was purified and an antibody was prepared. HSDJ mRNA was heat-induced in cultured cells. In pulse-labeling and chase experiments using COS-7 cells, the endogenous HSDJ homolog was pr...

journal_title：Journal of biochemistry

authors： Kanazawa M,Terada K,Kato S,Mori M

更新日期：1997-05-01 00:00:00

abstract：:Staring from low molecular weight RNA obtained from rainbow trout (Salmo gairdnerii) liver, 5S ribosomal RNA (rRNA) was highly purified by successive chromatography on columns of DEAE-Sephadex A50 and Sephadex G100. Products of complete and partial digestions on this RNA with pancreatic ribonuclease (RNase A) [EC 3.1....

journal_title：Journal of biochemistry

authors： Komiya H,Takemura S

更新日期：1979-10-01 00:00:00

abstract：:We have cloned a chitinase cDNA (MpChi-1) from plantain (Musa paradisiacal L) using rapid amplification of cDNA ends (RACE) according to a sequence fragment which we had cloned using the suppression subtractive hybridization (SSH) technique. The MpChi-1 encodes a protein of 326 amino acids and belongs to acidic chitin...

journal_title：Journal of biochemistry

authors： Fan J,Wang H,Feng D,Liu B,Liu H,Wang J

更新日期：2007-11-01 00:00:00

abstract：:Inositol 1,4,5-trisphosphate (InsP3) releases Ca2+ from the non-mitochondrial Ca2+ store site of various types of cells. To study the mechanisms of the Ca2+ release from the store site, the effect of InsP3 on the passive Ca2+ release and influx, and the active Ca2+ uptake in the presence of oxalate, was examined using...

journal_title：Journal of biochemistry

authors： Hirata M,Kukita M,Sasaguri T,Suematsu E,Hashimoto T,Koga T

更新日期：1985-06-01 00:00:00

abstract：:A chitinase was purified from the culture filtrate of Streptomyces erythraeus (SE). The enzyme (SE chitinase) has a molecular weight of 30,000 and pI 3.7, and shows optimal activity at pH 5.0 with an optimal ionic strength of less than 0.2 M NaCl. SE chitinase could hydrolyze chitin and its derivatives, but could not ...

journal_title：Journal of biochemistry

authors： Hara S,Yamamura Y,Fujii Y,Mega T,Ikenaka T

更新日期：1989-03-01 00:00:00

abstract：:Knockout mice lacking glycosyltransferases or sulfotransferases provide unequivocal evidence that the carbohydrate moieties of glycoproteins, glycolipids, and proteoglycans play essential roles in various biological phenomena such as development, the immune response, and tissue functions. Examples of abnormalities of ...

journal_title：Journal of biochemistry

authors： Muramatsu T

更新日期：2000-02-01 00:00:00

abstract：:Connectin (titin) links the Z line to the myosin filament in sarcomeres of vertebrate skeletal muscle. An 800 kDa fragment of alpha-connectin runs from the Z line up to the N2 line region in the I band, and the following beta-connectin portion runs up to the edge of the M line on the myosin filament in chicken breast ...

journal_title：Journal of biochemistry

authors： Kawamura Y,Kume H,Itoh Y,Ohtsuka S,Kimura S,Maruyama K

更新日期：1995-01-01 00:00:00

abstract：:Chalcone synthase was purified to homogeneity by polyacrylamide gel electrophoresis from cell suspension cultures of carrot in which anthocyanin synthesis was induced by transferring the cells from a medium containing 2,4-dichlorophenoxy-acetic acid (2,4-D) to one lacking it. A molecular weight of 80,000-85,000 for th...

journal_title：Journal of biochemistry

authors： Ozeki Y,Sakano K,Komamine A,Tanaka Y,Noguchi H,Sankawa U,Suzuki T

更新日期：1985-07-01 00:00:00

abstract：:1. Native human hemoglobin A was digested with proteases after treatment with reduced or oxidized glutathione, cysteine, or cystine. 2. The treatment of hemoglobin with a 40-fold molar excess of reduced glutathione or cysteine at 37 degrees C for 4 enhanced its susceptibility to trypsin 4 or 6 times, respectively. How...

journal_title：Journal of biochemistry

authors： Kimura H,Murata H,Uematsu H

更新日期：1980-08-01 00:00:00

abstract：:The pH dependence of the binding constant of Ca2+ to a phospholipase A2 of Agkistrodon halys blomhoffii, in which the alpha-amino group had been selectively modified to an alpha-keto group, was studied at 25 degrees C and ionic strength 0.1 by the tryptophyl fluorescence method. The dependence was compared with the re...

journal_title：Journal of biochemistry

authors： Haruki H,Teshima K,Samejima Y,Kawauchi S,Ikeda K

更新日期：1986-01-01 00:00:00

abstract：:The stimulatory effects of glucose and lactate on protein synthesis by round spermatids (steps 1-8) were further studied. When the cells were incubated with lactate, the response of protein synthesis in round spermatids was closely related to the intracellular level of ATP. The ATP level in spermatids increased to 3.1...

journal_title：Journal of biochemistry

authors： Nakamura M,Hino A,Kato J

更新日期：1981-10-01 00:00:00

abstract：:Serine dehydrogenase from Escherichia coli is a homotetrameric enzyme belonging to the short-chain dehydrogenase/reductase (SDR) family. This enzyme catalyses the NADP(+)-dependent oxidation of serine to 2-aminomalonate semialdehyde. The enzyme shows a stereospecificity for β-(3S)-hydroxy acid as a substrate; however,...

journal_title：Journal of biochemistry

authors： Yamazawa R,Nakajima Y,Mushiake K,Yoshimoto T,Ito K

更新日期：2011-06-01 00:00:00

abstract：:Site-specific recognition and DNA-binding activity of p53 are crucial for its tumour suppressor function. Previous reports have shown that metal ions can affect the specific recognition and DNA-binding activity of p53DBD. Here we firstly report that magnesium ion can bind to the protein and influence its DNA-binding a...

journal_title：Journal of biochemistry

authors： Xue Y,Wang S,Feng X

更新日期：2009-07-01 00:00:00

abstract：:The effects of D-glucose anomers on the germination of dormant spores of Bacillus megaterium QM B1551 were studied, alpha-D-Glucose (1 mM) slightly initiated the germination of the dormant spores during 10 min incubation at 37 degrees C, while about 60% of the dormant spores became germinated with beta-D-glucose (1 mM...

journal_title：Journal of biochemistry

authors： Miwa I,Ishihara H,Fujii H,Nagano H,Okuda J

更新日期：1979-01-01 00:00:00

abstract：:Four distinctive DNA polymerases have been isolated from mature oocytes of the starfish, Asterina pectinifera, and characterized. The N-ethylmaleimide-resistant DNA polymerase activity was separated from the other three DNA polymerases by hydroxyapatite column chromatography. The enzyme, whose approximate sedimentatio...

journal_title：Journal of biochemistry

authors： Haraguchi T,Nagano H

更新日期：1983-03-01 00:00:00

abstract：:Tryptophan hydroxylase from mouse mastocytoma P-815 is activated by ethylenediaminetetraacetate (EDTA). The activation proceeds in a pH-, temperature-, and time-dependent manner and leads to a 30-fold higher activity at maximum. The optimal EDTA concentration is 10 microns. The activation requires solely EDTA with des...

journal_title：Journal of biochemistry

authors： Yanagisawa M,Hasegawa H,Ichiyama A

更新日期：1982-08-01 00:00:00

abstract：:A method for immobilizing oligosaccharides on a TLC plate for immunostaining has been developed. N-Glycolylneuraminic acid (NeuGc)-containing oligosaccharides derived from II3NeuGc-LacCer, IV3NeuGc-nLcOse4Cer, II3NeuGc-GgOse3Cer, and II3(NeuGc)2-LacCer by digestion with our newly isolated endoglycoceramidase (Ito, M. ...

journal_title：Journal of biochemistry

authors： Higashi H,Hirabayashi Y,Ito M,Yamagata T,Matsumoto M,Ueda S,Kato S

更新日期：1987-08-01 00:00:00

abstract：:The association constants of hen egg yolk riboflavin binding protein with 8-substituted riboflavins were measured using a fluorometric titration method in the range of 10-40 degrees C, and thermodynamic parameters were calculated using ordinary methods. The flavins used were riboflavins whose 8-methyl group was substi...

journal_title：Journal of biochemistry

authors： Matsui K,Sugimoto K,Kasai S

更新日期：1982-04-01 00:00:00

abstract：:Paranitrophenylphosphate (pNPP) induced fluorescence changes in fluorescence isothiocyanate (FITC)-labeled Na+,K(+)-ATPase preparations. The extents of changes were similar to those induced by acetylphosphate (AcP) accompanying accumulation of a K(+)-sensitive phosphoenzyme (E2P) and an ouabain bound phosphoenzyme in ...

journal_title：Journal of biochemistry

authors： Yamazaki A,Kaya S,Tsuda T,Araki Y,Hayashi Y,Taniguchi K

更新日期：1994-12-01 00:00:00

abstract：:Interferon stimulated gene factor 3 (ISGF3) is a trimeric transcription factor activated on treatment of cells with interferon-alpha and beta (type I IFNs). Upon stimulation, the regulatory subunits, p84/91 and p113, present in the cytoplasm are phosphorylated at specific tyrosine residues and assemble with the DNA bi...

journal_title：Journal of biochemistry

authors： Suhara W,Yoneyama M,Yonekawa H,Fujita T

更新日期：1996-02-01 00:00:00