Purification and properties of 3-ketovalidoxylamine A C-N lyase from Flavobacterium saccharophilum.

abstract：:We have found that arachidonic (20 : 4) acid is indetectable in phosphatidylinositol and diacyglycerol extracted from Drosophila heads. After careful examinations of the lipid extraction processes and fatty acid detection system (gas-liquid chromatography), we excluded the possibility of the oxidation of polyunsaturat...

journal_title：Journal of biochemistry

authors： Yoshioka T,Inoue H,Kasama T,Seyama Y,Nakashima S,Nozawa Y,Hotta Y

更新日期：1985-09-01 00:00:00

abstract：:Partition of merocyanine dyes, which have a negative charge, onto photosynthetic membranes of chloroplasts and bacteria was analyzed by measuring the fluorescence intensity change, absorbance change, and amount of dye in the supernatant after centrifugation. The partition depended on the surface potential, which is a ...

journal_title：Journal of biochemistry

authors： Masamoto K,Matsuura K,Itoh S,Nishimura M

更新日期：1981-02-01 00:00:00

abstract：:p40(phox) of the phagocyte NADPH oxidase forms a complex with p67(phox) in cytosol, and coincidentally decreases in patients who lack p67(phox). Here we investigated the mode of translocation of p40(phox) to the membrane, its cytoskeletal localization on activation of the NADPH oxidase, and the dependency of its expre...

journal_title：Journal of biochemistry

authors： Tsunawaki S,Yoshikawa K

更新日期：2000-11-01 00:00:00

abstract：:8-Azido GTP (8-N3 GTP) was demonstrated to be polymerized into RNA by influenza virus-associated RNA polymerase at about one tenth the rate of GTP incorporation. The Km value for the azido analogue of GTP in primer-dependent RNA synthesis was 94 microM whereas Km for the natural substrate, GTP, was 6.7 microM. Upon ex...

journal_title：Journal of biochemistry

authors： Asano Y,Mizumoto K,Maruyama T,Ishihama A

更新日期：1995-03-01 00:00:00

abstract：:To investigate the function of the gamma-carboxyglutamic acid (Gla) residues of factor IXa in the activation of factor X, a new species of bovine factor IXa, designated "factor IXa beta'," and its corresponding Gla-domainless form, designated "Gla-domainless factor IXa beta'," were prepared under controlled conditions...

journal_title：Journal of biochemistry

authors： Morita T,Fukudome K,Miyata T,Iwanaga S

更新日期：1991-12-01 00:00:00

abstract：:A cyclodextrin glucanotransferase (CGTase) from Bacillus clarkii 7364 converts starch into gamma-cyclodextrin (gamma-CD) with high specificity. Comparison of the deduced amino acid sequence of this CGTase with those of other typical CGTases revealed that several amino acids are deleted or substituted with others at se...

journal_title：Journal of biochemistry

authors： Nakagawa Y,Takada M,Ogawa K,Hatada Y,Horikoshi K

更新日期：2006-09-01 00:00:00

abstract：:We have reported that Erbin expression was down-regulated in the Jurkat leukaemia T lymphocytes treated with the recombinant soluble tumour necrosis factor-related apoptosis-inducing ligand (rsTRAIL). Herein, we studied the expression and the regulation of Erbin and its binding partner, ErbB2, in the MCF-7 breast canc...

journal_title：Journal of biochemistry

authors： Liu N,Zhang J,Zhang J,Liu S,Liu Y,Zheng D

更新日期：2008-06-01 00:00:00

abstract：:Inhibitory PAS domain protein (IPAS) is a dual function protein acting as a transcriptional repressor and as a pro-apoptotic protein. Simultaneous dual-color single-molecule imaging of EGFP-IPAS coexpressed with Mit-TagRFP-T in living HeLa cells revealed that fraction of EGFP-IPAS was arrested in the nucleus and on mi...

journal_title：Journal of biochemistry

authors： Kasai S,Kajimoto S,Ito Y,Saito T,Yasumoto KI,Tokunaga M,Sakata-Sogawa K,Fukumura H,Sogawa K

更新日期：2017-03-01 00:00:00

abstract：:Do non-active-site residues participate in protein function in a more direct way than just by holding the static framework of the protein molecule? If so, how important are they? As a model to answer these questions, ATB17, which is a mutant of aspartate aminotransferase created by directed evolution, is an ideal syst...

journal_title：Journal of biochemistry

authors： Shimotohno A,Oue S,Yano T,Kuramitsu S,Kagamiyama H

更新日期：2001-06-01 00:00:00

abstract：:To improve our understanding of the physiological roles of parvalbumins, PA-1 (pI 4.78) and PA-2 (pI 4.97) parvalbumins were prepared from bullfrog skeletal muscle and their calcium binding properties were examined in a medium of constant ionic strength (I = 0.106, pH 6.80, at 20 degrees C) containing various concentr...

journal_title：Journal of biochemistry

authors： Ogawa Y,Tanokura M

更新日期：1986-01-01 00:00:00

abstract：:The immunological properties of acid proteinases from rat spleen, two types of cathepsin D and a cathepsin E-like enzyme, were examined. The rabbit antiserum was prepared against the major form of cathepsin D (cathepsin D-I) from rat spleen. The antiserum quantitatively precipitated the enzyme activity from the purifi...

journal_title：Journal of biochemistry

authors： Yamamoto K,Kamata O,Katsuda N,Kato K

更新日期：1980-02-01 00:00:00

abstract：:It is known that nucleoporins, a family of glycoproteins with N-acetylglucosamine that are found in nuclear pore complexes, are essential for nuclear import and export. A major component of the family, p62, was purified from a salt extract of rat liver nuclear envelopes by wheat germ agglutinin-Sepharose affinity chro...

journal_title：Journal of biochemistry

authors： Kita K,Omata S,Horigome T

更新日期：1993-03-01 00:00:00

abstract：:The fructose 2,6-bisphosphate concentrations in unwashed, washed, and leukocyte-free erythrocytes were compared. The concentration in washed red cells was 31 +/- 15 pmol per ml of cells (mean +/- S.D., n = 6). The concentration in unwashed erythrocytes was at least twofold higher, but the value in washed red cells was...

journal_title：Journal of biochemistry

authors： Moriyasu M,Yamamoto M,Hamasaki N

更新日期：1988-06-01 00:00:00

abstract：:Glutamine synthetase from a Gram-positive acid-fast bacterium, Mycobacterium smegmatis, was purified to homogeneity from cells grown with glycerol-bouillon medium. Electron micrographs of the enzyme revealed a dodecameric arrangement of its subunits in two superimposed hexagonal rings, similar to the structure of glut...

journal_title：Journal of biochemistry

authors： Kimura K,Yagi K,Matsuoka K

更新日期：1984-06-01 00:00:00

abstract：:The Basidiomycete ras gene possesses a pyrimidine-rich stretch (CT-motif) with a short (7 bases) mirror repeat in which its major transcription start point is contained. To analyze the tertiary structure induced by the CT/AG-biased sequence and its effect on gene expression in supercoiled plasmids in Escherichia coli,...

journal_title：Journal of biochemistry

authors： Yamazaki T,Hasebe T,Shouguchi J,Amano H,Kajiwara S,Shishido K

更新日期：1997-10-01 00:00:00

abstract：:The phi X174-DNA membrane complex was isolated from Escherichia coli infected with phi X174 am3 by isopycnic sucrose gradient centrifugation followed by zone electrophoresis. The phi X174 DNA-membrane complex banded at two positions, intermediate density membrane fraction and cytoplasmic membrane fraction, having bouy...

journal_title：Journal of biochemistry

authors： Azuma J,Morita J,Komano T

更新日期：1980-08-01 00:00:00

abstract：:Rat and mouse cDNAs for Zn-alpha 2-glycoprotein (Zn alpha 2gp) were isolated from liver libraries (lambda gt11) and compared with the human one. The lengths of cDNA inserts analyzed were 1,233 and 1,273 nucleotides for rat and mouse, respectively. The deduced amino acid sequences suggested that rat and mouse Zn alpha ...

journal_title：Journal of biochemistry

authors： Ueyama H,Naitoh H,Ohkubo I

更新日期：1994-09-01 00:00:00

abstract：:The oxidation of equivalent concentrations of phospholipids in homogeneous solution, in multilamellar liposomal suspension, and in rat liver homogenate was carried out under aerobic conditions at 37 degrees C in order to examine the biochemical fate of oxidized phospholipids. Rat liver phospholipids were extracted wit...

journal_title：Journal of biochemistry

authors： Kambayashi Y,Yamashita S,Niki E,Yamamoto Y

更新日期：1997-03-01 00:00:00

abstract：:The membrane protein syntaxin (originally named HPC-1) is involved in vesicle trafficking and required for neurotransmitter release at nerve terminals. The presence of syntaxin on target membranes is hypothesized to confer specificity to targeting and fusion via interactions with complementary vesicle-associated prote...

journal_title：Journal of biochemistry

authors： Watanabe T,Fujiwara T,Komazaki S,Yamaguchi K,Tajima O,Akagawa K

更新日期：1999-04-01 00:00:00

abstract：:The receptor-binding assay for cAMP was improved by using polyethylenimine-treated glass filters. A polyethylenimine-treated glass filter has high protein binding capacity. This high capacity allows an increase in the amount of protein per assay tube and the use of a crude preparation, such as a beef heart extract, as...

journal_title：Journal of biochemistry

authors： Takeda T,Kuno T,Shuntoh H,Tanaka C

更新日期：1989-02-01 00:00:00

abstract：:We have previously developed a simple gene transfection procedure mediated by cationic lipid vesicles for animal cells, in which a commercially available cationic surfactant, dimethyldioctadecyl ammonium bromide (DDAB), was used for making lipid vesicles. In the present study, we examined enhancement of transfection e...

journal_title：Journal of biochemistry

authors： You J,Kamihira M,Iijima S

更新日期：1999-06-01 00:00:00

abstract：:When the membrane fraction of Saccharomyces carlsbergensis was incubated with radiolabeled isopentenyl diphosphate in the presence of farnesyl diphosphate and Mg2+, phosphorylated and free long-chain polyprenols were formed. The reaction was inhibited by EDTA and heavy metal cations. A series of non-ionic detergents w...

journal_title：Journal of biochemistry

authors： Bukhtiyarov YE,Shabalin YA,Kulaev IS

更新日期：1993-06-01 00:00:00

abstract：:Chondroitin sulphate proteoglycan 4 (CSPG4) is a cell surface proteoglycan highly expressed by tumour, perivascular and oligodendrocyte cells and known to be involved cell adhesion and migration. This study showed that CSPG4 was present as a proteoglycan on the cell surface of two melanoma cell lines, MM200 and Me1007...

journal_title：Journal of biochemistry

authors： Tang F,Lord MS,Stallcup WB,Whitelock JM

更新日期：2018-05-01 00:00:00

abstract：:A large number of prokaryotic as well as eukaryotic proteins are produced with amino terminal propeptides. These amino-terminal extensions are essential for mediating proper folding of their corresponding proteins and are also termed as intramolecular chaperones. Though these propeptides are highly specific and unique...

journal_title：Journal of biochemistry

authors： Shinde U,Inouye M

更新日期：1994-04-01 00:00:00

abstract：:Erucic acid was incorporated into cardiac phosphatidylserine and hepatic and cardiac sphingomyelin of male Sprague-Dawley rat. The fatty acid compositions of mitochondrial and microsomal phospholipids were similar in both liver and heart. The effect of a low fat diet and a diet containing erucic acid on the fatty acid...

journal_title：Journal of biochemistry

authors： Ishinaga M,Sato J,Kitagawa Y,Sugimoto E,Kito M

更新日期：1982-07-01 00:00:00

abstract：:The ATP requirement for two steps of DNA replication, the synthesis and subsequent joining of Okazaki fragments, was investigated by using isolated HeLa cell nuclei. Among adenine nucleotides tested, high levels of dATP and ADP stimulated DNA synthesis. In the presence of high levels of ATP, the addition of high level...

journal_title：Journal of biochemistry

authors： Enomoto T,Tanuma S,Yamada MA

更新日期：1981-03-01 00:00:00

abstract：:Long interspersed nuclear element (LINE) is known to be transposed by reverse transcription using its RNA transcript. Recognition of the 3' stem-loop of LINE RNA by its reverse transcriptase (RT) is an important step of the retrotransposition. Our previous study revealed that the second G residue (G8) in the GGAUA loo...

journal_title：Journal of biochemistry

authors： Otsu M,Kajikawa M,Okada N,Kawai G

更新日期：2017-10-01 00:00:00

abstract：:Mammalian/mechanistic target of rapamycin (mTOR) is a highly conserved serine/threonine kinase that responds to environmental determinants such as growth factor concentration, nutrient availability, energy sufficiency and stress. mTOR forms two different complexes, called mTOR complex 1 (mTORC1) and mTORC2, and these ...

journal_title：Journal of biochemistry

authors： Hoshii T,Matsuda S,Hirao A

更新日期：2014-08-01 00:00:00

abstract：:We have developed a novel method for the preparation of 'recombinant proteoliposomes'. Membrane proteins were expressed on budded virus (BV) envelopes using baculovirus gene expression systems, and proteoliposomes were prepared by fusion of these viruses with liposomes. First, plasmid DNA containing the gene for the t...

journal_title：Journal of biochemistry

authors： Fukushima H,Mizutani M,Imamura K,Morino K,Kobayashi J,Okumura K,Tsumoto K,Yoshimura T

更新日期：2008-12-01 00:00:00

abstract：:Three forms of rat brain enolase (Fletcher, L., Rider, C.C., & Taylor, C.B. (1976) Biochim. Biophys. Acta 452, 245-252), which were separable by DEAE-cellulose chromatography (referred as enolase I, enolase II, and enolase III in order of the elution), were purified with a high yield by use of column chromatographies ...

journal_title：Journal of biochemistry

authors： Suzuki F,Umeda Y,Kato K

更新日期：1980-06-01 00:00:00