Fructose 2,6-bisphosphate in human erythrocytes.

abstract：:Binding of native connectin (2,100 kDa fragment of alpha-connectin) to myosin filaments was investigated using a sedimentation technique and densitometric estimations of the separated proteins. In the presence of 60 mM KCl and 5 mM phosphate buffer, pH 7.0, as much as 1.5 mol of connectin was bound to 1 mol of myosin,...

journal_title：Journal of biochemistry

authors： Murayama T,Nakauchi Y,Kimura S,Maruyama K

更新日期：1989-02-01 00:00:00

abstract：:An actin-depolymerizing marine natural product, mycalolide B, and a related compound, kabiramide D, were labeled with biocytin, a biotin derivative, and used to specify target molecules in cultured rat 3Y1 fibroblasts. Mycalolide B exhibited the ability to bind to various intracellular proteins, probably through the M...

journal_title：Journal of biochemistry

authors： Wada S,Matsunaga S,Saito S,Fusetani N,Watabe S

更新日期：1998-05-01 00:00:00

abstract：:Tryptophan hydroxylase from mouse mastocytoma P-815 is activated by ethylenediaminetetraacetate (EDTA). The activation proceeds in a pH-, temperature-, and time-dependent manner and leads to a 30-fold higher activity at maximum. The optimal EDTA concentration is 10 microns. The activation requires solely EDTA with des...

journal_title：Journal of biochemistry

authors： Yanagisawa M,Hasegawa H,Ichiyama A

更新日期：1982-08-01 00:00:00

abstract：:The allosteric properties of AMP deaminase [EC 3.5.4.6] from chicken erythrocytes have been qualitatively and quantitatively accounted for by the concerted transition theory of Monod et al., on the assumption that this enzyme has different numbers of binding sites for each ligand. Theoretical curves yield a satisfacto...

journal_title：Journal of biochemistry

authors： Yoshino M,Kawamura Y,Ogasawara N

更新日期：1976-08-01 00:00:00

abstract：:Three forms of cytochrome P-450 of liver microsomes of 3-methylcholanthrene-treated Golden hamsters were purified and characterized as regards their catalytic activity toward aflatoxin B1-related hepatocarcinogenic mycotoxins. These include two major forms, designated as cytochrome P-450-AFB (P-450-I) and P-450-II, an...

journal_title：Journal of biochemistry

authors： Fukuhara M,Nohmi T,Mizokami K,Sunouchi M,Ishidate M Jr,Takanaka A

更新日期：1989-08-01 00:00:00

abstract：:LipB, the lipase activator protein of Pseudomonas aeruginosa TE3285, was overproduced in Escherichia coli, and purified 4.9-fold over the crude extract in the presence of SDS. The purified LipB reactivated the lipase from P. aeruginosa TE3285 denatured with guanidine hydrochloride, and its reactivation did not involve...

journal_title：Journal of biochemistry

authors： Shibata H,Kato H,Oda J

更新日期：1998-01-01 00:00:00

abstract：:Isolation and characterization of a fibrous protein component which might be associated with contractile ring of a dividing Tetrahymena cell were attempted by making use of coprecipitation of the protein with rabbit skeletal muscle myosin. The protein was purified to homogeneity by ammonium sulfate fractionation betwe...

journal_title：Journal of biochemistry

authors： Numata O,Yasuda T,Hirabayashi T,Watanabe Y

更新日期：1980-11-01 00:00:00

abstract：:Stem cells divide and undergo self-renewal depending on the signals received from the stem cell niche. This phenomenon is indispensable to maintain tissues and organs in individuals. However, not all the molecular factors and mechanisms of self-renewal are known. In our previous study, we reported that glycosylphospha...

journal_title：Journal of biochemistry

authors： Rikitake M,Matsuda A,Murata D,Dejima K,Nomura KH,Abbott KL,Mitani S,Nomura K

更新日期：2020-12-26 00:00:00

abstract：:Bromoacetamide derivatives having n-alkyl substituents (BrCH2CONH(CH2)nH, 1-n) and carboxyalkyl substituents (BrCH2CONH(CH2)nCOOH, 2-n) react with His-15 in lysozyme exclusively at N epsilon 2 at pH 5.5 and 40 degree C. Kinetic studies suggest that lysozyme has a small hydrophobic pocket that binds these reagents in t...

journal_title：Journal of biochemistry

authors： Yamada H,Uozumi F,Ishikawa A,Imoto T

更新日期：1984-02-01 00:00:00

abstract：:The binding of ADP to subfragment-1 was investigated by the gel filtration method. The amount of bound ADP was determined as a function of free ADP concentration. Linear Scatchard plots were obtained. The maximum binding number, 0.55 mole of ADP per 10(5) g of protein, and the dissociation constant, 1.6 x 10(-6) M, we...

journal_title：Journal of biochemistry

authors： Yoshida M,Morita F

更新日期：1975-05-01 00:00:00

abstract：:The effects of various compounds on ascorbate-2-sulfate sulfohydrolase and arylsulfatase (EC 3.1.6.1) activities in the copurified preparation from the liver of Charonia lampas were investigated. The former activity was competively inhibited by inorganic phosphate and sulfate. The latter was not affected by sulfate....

journal_title：Journal of biochemistry

authors： Hatanaka H,Ogawa Y,Egami F

更新日期：1975-04-01 00:00:00

abstract：:The readthrough of premature termination codon (PTC) by ribosome sometimes produces full-length proteins. We previously reported a readthrough of PTC of glycosyltransferase gene B4GALNT1 with hereditary spastic paraplegia (HSP). Here we featured the readthrough of B4GALNT1 of two mutants, M4 and M2 with PTC by immunob...

journal_title：Journal of biochemistry

authors： Yesmin F,Bhuiyan RH,Ohmi Y,Ohkawa Y,Tajima O,Okajima T,Furukawa K,Furukawa K

更新日期：2020-08-01 00:00:00

abstract：:Both rabbit liver microsomes and reconstituted system with purified cytochrome P-450 and cofactors enzymatically oxidized o-dithiane (1, 2-dithiane), 3-methyl-o-dithiane, thiane and 2-methylthiane to the corresponding mono-oxygenated products; sulfides or disulfides were oxidized to the corresponding sulfoxides or thi...

journal_title：Journal of biochemistry

authors： Fukushima D,Kim YH,Iyanagi T,Oae S

更新日期：1978-04-01 00:00:00

abstract：:A Ca2+-sensitive electrode was used for determination of the binding strength of Ca2+ to bovine alpha-lactalbumin in 60 mM Tris buffer (pH 7.8-8.5) in the presence of various concentrations of NaCl. The dependence of the apparent binding constant on the concentration of NaCl was consistent with competitive binding of ...

journal_title：Journal of biochemistry

authors： Hamano M,Nitta K,Kuwajima K,Sugai S

更新日期：1986-12-01 00:00:00

abstract：:The contribution of the P1' residue at the first reactive site of peanut protease inhibitor B-III to the inhibition was analyzed by replacement of the P1' Arg(11) with other amino acids (Arg, Ser, Ala, Leu, Phe, Asp) after selective modification of the second reactive site. The Arg derivative had the same trypsin inhi...

journal_title：Journal of biochemistry

authors： Kurokawa T,Hara S,Norioka S,Teshima T,Ikenaka T

更新日期：1987-03-01 00:00:00

abstract：:Pig heart phosphoprotein phosphatase [phosphoprotein phosphophydrolase, EC 3.1.3.16] of Mr 224,000 was dissociated by gel-filtration on Sephacryl S-300, into an active subunit (alpha subunit) of Mr 31,000 and inactive subunits of higher molecular weight in the presence of 6 M urea. After the removal of urea, these sub...

journal_title：Journal of biochemistry

authors： Imazu M,Imaoka T,Usui H,Kinohara N,Takeda M

更新日期：1981-09-01 00:00:00

abstract：:beta-Glycosidase from the extreme thermophilic archaeon Sulfolobus solfataricus is a tetrameric protein with a molecular mass of 240 kDa, stable in the presence of detergents, and with a maximal activity at temperatures above 95 degrees C. Understanding the structure-activity relationships of the enzyme under differen...

journal_title：Journal of biochemistry

authors： D'Auria S,Nucci R,Rossi M,Bertoli E,Tanfani F,Gryczynski I,Malak H,Lakowicz JR

更新日期：1999-09-01 00:00:00

abstract：:Sliding machines composed of F-actin and myosin or microtubules and kinesin or dynein convert the free energy of ATP hydrolysis into sliding movements and mechanical work. Development of optical microscopy with micromanipulation techniques has made possible direct observation of single events of sliding exhibited by s...

journal_title：Journal of biochemistry

authors： Oosawa F

更新日期：1995-11-01 00:00:00

abstract：:A comparative study of vertebrate ventricle tropomyosin has been carried out from the viewpoint of molecular evolution. The ventricles containing one-component tropomyosin were generally known, and in this paper those containing two components were also found in 8 species among mammals, reptiles, amphibia, and fish, b...

journal_title：Journal of biochemistry

authors： Yamane A,Hirabayashi T

更新日期：1985-05-01 00:00:00

abstract：:c-Abl non-receptor tyrosine kinase has been implicated in many cellular processes including cell differentiation, stress response and regulating gene transcription. The mechanism by which c-Abl is involved in the regulation of gene transcription remains to be elucidated. In this study, we investigated the functions of...

journal_title：Journal of biochemistry

authors： Jing Y,Wang M,Tang W,Qi T,Gu C,Hao S,Zeng X

更新日期：2007-05-01 00:00:00

abstract：:The sarcoplasmic reticulum (SR) membranes of rabbit skeletal muscle were allowed to react with N-(3-pyrene)maleimide (PMI) at pH 7 at 30 degrees C. The Ca(2+)-transporting activity of the SR membranes was reduced to 20% when PMI was bound to the extent of 1 mol/mol of Ca(2+)-transporting ATPase. The ATPase and the E-P...

journal_title：Journal of biochemistry

authors： Suzuki T,Kawakita M

更新日期：1993-08-01 00:00:00

abstract：:MutM protein, which removes the oxidatively damaged DNA base product, 8-oxoguanine (GO), has been crystallized by means of a hanging-drop vapor-diffusion procedure using polyethyleneglycol monomethylether 2000 as a precipitant in 2-(cyclohexylamino) ethanesulfonic acid (CHES) buffer, pH 9.8. The diffraction data deriv...

journal_title：Journal of biochemistry

authors： Sugahara M,Mikawa T,Kato R,Fukuyama K,Kumasaka T,Yamamoto M,Inoue Y,Kuramitsu S

更新日期：2000-01-01 00:00:00

abstract：:To study how collagen synthesis is regulated in developing chick embryonic skin, hydroxyproline synthesis, incorporation of proline, and translational activity and content of collagen mRNA in 12-, 15-, and 18-day skins were determined and compared with each other. Hydroxyproline synthesis in the 18-day skins was marke...

journal_title：Journal of biochemistry

authors： Ohyama K,Tokimitsu I,Tajima S

更新日期：1991-08-01 00:00:00

abstract：:Purothionin isolated from commercial wheat flour contained several components and two of them (A-I and A-II) were isolated in pure form by CM-52 column chromatography. Each component contained 45 amino acid residues with a 4 disulfide bonds. Purothionin A-II was digested with trypsin and thermolysin to isolate cystine...

journal_title：Journal of biochemistry

authors： Hase T,Matsubara H,Yoshizumi H

更新日期：1978-06-01 00:00:00

abstract：:When the competitive inhibitor benzeneboronic acid (BBA) forms a complex with alpha-chymotrypsin [EC 3.4.21.1] protons are released in the acidic pH region. The proton release can be measured by a difference potentiometric technique. The proton release is also observed in chymotrypsinogen A but not in TRCK-, DIP-, and...

journal_title：Journal of biochemistry

authors： Hanai K

更新日期：1976-01-01 00:00:00

abstract：:Mannan, a ligand for the mannose/N-acetylglucosamine (GlcNAc) receptor, induces suppression of oxygen consumption and increases glucose production in the perfused rat liver, and repeated infusion of mannan causes desensitization of the responses. In this study, we examined whether activation of Kupffer cells by endoto...

journal_title：Journal of biochemistry

authors： Kimura K,Hamada M,Moriyama M,Kannan Y,Shiota M,Sakurada K,Musashi M,Sugano T

更新日期：1996-09-01 00:00:00

abstract：:Several proteins in the mammalian endoplasmic reticulum are substrates for protein kinases. Many unidentified phosphoproteins from this compartment are described in the literature, and this prompted us to try to identify at least the more dominant ones. When solubilized bovine and murine microsomes were phosphorylated...

journal_title：Journal of biochemistry

authors： Janson IM,Ek B,Ek P

更新日期：1997-01-01 00:00:00

abstract：:Dimer formation of HIV-1 genomic RNA through its dimerization initiation site (DIS) is crucial to maintaining infectivity. Two types of dimers, the initially generated kissing-loop dimer and the subsequent product of the extended-duplex dimer, are formed in the stem-bulge-stem region with a loop including a self-compl...

journal_title：Journal of biochemistry

authors： Baba S,Takahashi K,Noguchi S,Takaku H,Koyanagi Y,Yamamoto N,Kawai G

更新日期：2005-11-01 00:00:00

abstract：:The stalk protein L12 is the only multiple component in 50S ribosomal subunit. In Escherichia coli, two L12 dimers bind to the C-terminal domain of L10 to form a pentameric complex, L10[(L12)(2)](2), while the recent X-ray crystallographic study and tandem MS analyses revealed the presence of a heptameric complex, L10...

journal_title：Journal of biochemistry

authors： Nomura T,Nakatsuchi M,Sugita D,Nomura M,Kaminishi T,Takemoto C,Shirouzu M,Miyoshi T,Yokoyama S,Hachimori A,Uchiumi T

更新日期：2008-11-01 00:00:00

abstract：:Cholecalciferol 25-hydroxylase was partially purified by polyethylene glycol fractionation and chromatographies on octylamino-Sepharose and hydroxylapatite columns starting from the liver microsomes of female rats, and compared with P-450cc25 purified from the liver microsomes of male rats (Hayashi, et al. (1986) J. B...

journal_title：Journal of biochemistry

authors： Hayashi S,Usui E,Okuda K

更新日期：1988-05-01 00:00:00