Actin-binding specificity of marine macrolide toxins, mycalolide B and kabiramide D.

abstract：:The bacterial opsins can be refolded to regenerate the chromophore by transfer from SDS to DMPC/CHAPS/SDS mixed micelles in the presence of retinal. A sequential refolding model has been proposed for bacterioopsin [Booth et al. (1995) Nature Struct. Biol. 2, 139-143]. However, the roles of DMPC and CHAPS in the refold...

journal_title：Journal of biochemistry

authors： Sugiyama Y,Mukohata Y

更新日期：1996-06-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the expression of phospholipid biosynthetic genes, including the INO1 gene (encoding inositol-1-phosphate synthase), is coordinately regulated by a cis-acting transcriptional element, UAS(INO) (inositol-sensitive upstream activating sequence). For this paper we studied the effect...

journal_title：Journal of biochemistry

authors： Kagiwada S,Zen R

更新日期：2003-04-01 00:00:00

abstract：:We have developed a novel method for the preparation of 'recombinant proteoliposomes'. Membrane proteins were expressed on budded virus (BV) envelopes using baculovirus gene expression systems, and proteoliposomes were prepared by fusion of these viruses with liposomes. First, plasmid DNA containing the gene for the t...

journal_title：Journal of biochemistry

authors： Fukushima H,Mizutani M,Imamura K,Morino K,Kobayashi J,Okumura K,Tsumoto K,Yoshimura T

更新日期：2008-12-01 00:00:00

abstract：:Two G-protein-coupled receptors (GPCRs) that couple with Gαq/11, B2 bradykinin (BK) receptor (B2R) and ATP/UTP receptor P2Y2 (P2Y2R), are ubiquitously expressed and responsible for vascular tone, inflammation, and pain. We analysed the cellular signalling of P2Y2Rs in cells that express B2Rs. B2R desensitization induc...

journal_title：Journal of biochemistry

authors： Yashima S,Shimazaki A,Mitoma J,Nakagawa T,Abe M,Yamada H,Higashi H

更新日期：2015-08-01 00:00:00

abstract：:Knockout mice lacking glycosyltransferases or sulfotransferases provide unequivocal evidence that the carbohydrate moieties of glycoproteins, glycolipids, and proteoglycans play essential roles in various biological phenomena such as development, the immune response, and tissue functions. Examples of abnormalities of ...

journal_title：Journal of biochemistry

authors： Muramatsu T

更新日期：2000-02-01 00:00:00

abstract：:The effects of 2'-substitutions of ATP on the substrate and inhibitor properties for RNA synthesis were studied in the poly(dAT)-dependent reaction of Escherichia coli RNA polymerase. In the presence of UTP, 2'-deoxy-2'-azidoadenosine 5'-triphosphate (AZTP) was incorporated into an acid-insoluble fraction at one-tenth...

journal_title：Journal of biochemistry

authors： Ishihama A,Enami M,Nishijima Y,Fukui T,Ohtsuka E,Ikehara M

更新日期：1980-03-01 00:00:00

abstract：:The incorporation of serine and ethanolamine into phospholipids in rabbit retinal subcellular fractions and in excised retinas was studied in vitro, and some enzymic properties of the incorporation of phospholipid bases by base exchange were examined in the microsomal fraction. The retina was found to have a higher ra...

journal_title：Journal of biochemistry

authors： Mizuno A

更新日期：1976-07-01 00:00:00

abstract：:Undecaprenyl diphosphate synthase catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall. This cis-type prenyltra...

journal_title：Journal of biochemistry

authors： Kharel Y,Zhang YW,Fujihashi M,Miki K,Koyama T

更新日期：2003-12-01 00:00:00

abstract：:Midkine (MK) is a 13-kDa heparin-binding growth/differentiation factor, and its interaction with heparan sulfate proteoglycan is important in promotion of neurite outgrowth. This study was performed to reveal the species of syndecans that interact with MK during embryogenesis of the central nervous system in the rat. ...

journal_title：Journal of biochemistry

authors： Nakanishi T,Kadomatsu K,Okamoto T,Ichihara-Tanaka K,Kojima T,Saito H,Tomoda Y,Muramatsu T

更新日期：1997-02-01 00:00:00

abstract：:The most abundant glycoprotein in the coelomic fluid of sea urchin Anthocidaris crassispina was purified and its subunit structure, molecular form in the native state, amino acid composition, and electron micrographic image were studied. The results showed that the protein in its native state was basically a tetramer ...

journal_title：Journal of biochemistry

authors： Giga Y,Ikai A

更新日期：1985-07-01 00:00:00

abstract：:Aberrant DNA methylation is a common form of epigenetic alterations and it has been proved to be closely related to many cancers, while its role in epidermal growth factor receptor (EGFR)-mutated non-small cell lung cancer (NSCLC) is not clear. This study focuses on the role of DNA methyltransferase 1 (DNMT1) in EGFR-...

journal_title：Journal of biochemistry

authors： Wu XY,Chen HC,Li WW,Yan JD,Lv RY

更新日期：2020-08-01 00:00:00

abstract：:Erucic acid was incorporated into cardiac phosphatidylserine and hepatic and cardiac sphingomyelin of male Sprague-Dawley rat. The fatty acid compositions of mitochondrial and microsomal phospholipids were similar in both liver and heart. The effect of a low fat diet and a diet containing erucic acid on the fatty acid...

journal_title：Journal of biochemistry

authors： Ishinaga M,Sato J,Kitagawa Y,Sugimoto E,Kito M

更新日期：1982-07-01 00:00:00

abstract：:Tetrahymena F-actin-binding protein, which induces bundling of Tetrahymena F-actin, was localized to a division furrow during cytokinesis. We report here the cloning and characterization of the gene and cDNA of a Tetrahymena F-actin-binding protein. The cDNA encodes a protein comprising 579 deduced amino acids with a ...

journal_title：Journal of biochemistry

authors： Watanabe A,Yonemura I,Gonda K,Numata O

更新日期：2000-01-01 00:00:00

abstract：:Changes in the fatty acid composition of choline glycerophospholipid (CGP) and ethanolamine glycerophospholipid (EGP) in the liver, heart, kidney, testis, spleen, and brain of rats were examined at various stages of streptozotocin-induced diabetes. Diabetes of prolonged duration (8 weeks) caused profound alterations i...

journal_title：Journal of biochemistry

authors： Hu Q,Ishii E,Nakagawa Y

更新日期：1994-03-01 00:00:00

abstract：:The absorption and fluorometric changes of the reaction mixture of luciferase-FMNH2 complex with O2 were re-examined. Rapid formation (k2(app) = 2.0 s-1 at [O2] = 120 micrometer) of an intermediate with a single absorption maximum at 380 nm within the range of 350-550 nm, and a weak fluorescence at 520 nm (less than o...

journal_title：Journal of biochemistry

authors： Ashizawa N,Nakamura T,Watanabe T

更新日期：1977-04-01 00:00:00

abstract：:Cytochromes b(561) are a family of transmembrane proteins found in most eukaryotic cells and contain two haem b prosthetic groups per molecule being coordinated with four His residues from four different transmembrane alpha-helices. Although cytochromes b(561) residing in the chromaffin vesicles has long been known to...

journal_title：Journal of biochemistry

authors： Nakanishi N,Takeuchi F,Tsubaki M

更新日期：2007-11-01 00:00:00

abstract：:The interaction of Pi with sarcoplasmic reticulum (SR) isolated from rabbit skeletal muscle was studied using bromocresol purple (BCP) as a probe and a dual-wavelength spectrophotometer. Two kinds of absorption-intensity changes controlled by a low concentration of Ca2+ (greater than 10(-6)M) were observed after addit...

journal_title：Journal of biochemistry

authors： Nakamaru Y,Nomura K

更新日期：1977-02-01 00:00:00

abstract：:To investigate the role of carboxyl groups of glucoamylases [EC 3.2.1.3] from a Rhizopus sp. (Gluc1 and Gluc2), the modification of Gluc1 and Gluc2 with a water-soluble carbodiimide, 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl)carbodiimide metho-p-toluenesulfonate (CMC), was studied. The inactivation of Gluc1 proceeded wi...

journal_title：Journal of biochemistry

authors： Iwama M,Ohtsuki R,Takahashi T,Irie M

更新日期：1984-08-01 00:00:00

abstract：:Inosine triphosphate (ITP) does not serve as a substrate for myosin light-chain kinase from gizzard muscle. That is to say, myosin light-chain is not phosphorylated in ITP media. Nevertheless, at pH 6.8, 1 mM or 5 mM ITP induces superprecipitation of skeletal acto-gizzard myosin. The ITP-induced superprecipitation occ...

journal_title：Journal of biochemistry

authors： Onishi H,Watanabe S

更新日期：1979-08-01 00:00:00

abstract：:The chromatin fraction of rat liver exhibited proteolytic activity caused by serine proteases, with maximal activity at pH 8 or 10. They were analyzed by affinity labeling with [3H]diisopropylfluorophosphate followed by SDS-polyacrylamide gel electrophoresis, and partially purified by gel filtration through Sepharose ...

journal_title：Journal of biochemistry

authors： Tsurugi K,Ogata K

更新日期：1982-11-01 00:00:00

abstract：:We previously reported that novel Mg(2+)-ATPases were induced in rat liver peroxisomes by clofibrate administration and that these activities consisted of at least two types of enzymes, N-ethylmaleimide (NEM)-sensitive and -resistant. Here we present evidence that neither of these major peroxisomal ATPases is associat...

journal_title：Journal of biochemistry

authors： Shimizu S,Imanaka T,Takano T,Ohkuma S

更新日期：1992-12-01 00:00:00

abstract：:The kinetics of alpha-crystallin reaggregation in the presence of calcium ions were investigated by monitoring changes in optical density at 320 nm. alpha-Crystallin showed a critical concentration. In the initial stages, reaggregation and deaggregation of alpha-crystallin may be essentially fully reversible in the pr...

journal_title：Journal of biochemistry

authors： Sano Y

更新日期：1980-09-01 00:00:00

abstract：:MutM protein, which removes the oxidatively damaged DNA base product, 8-oxoguanine (GO), has been crystallized by means of a hanging-drop vapor-diffusion procedure using polyethyleneglycol monomethylether 2000 as a precipitant in 2-(cyclohexylamino) ethanesulfonic acid (CHES) buffer, pH 9.8. The diffraction data deriv...

journal_title：Journal of biochemistry

authors： Sugahara M,Mikawa T,Kato R,Fukuyama K,Kumasaka T,Yamamoto M,Inoue Y,Kuramitsu S

更新日期：2000-01-01 00:00:00

abstract：:A major lipid class in the Harderian glands of the Mongolian gerbil was investigated. The IR and 1H-NMR spectra suggested that it was a wax-like compound. Fatty acids present were capric, lauric, myristic, palmitic, stearic, arachidic, and behenic acids in ratios of 3.0%, 16.3%, 16.5%, 29.5%, 5.1%, 16.4%, and 9.8%, re...

journal_title：Journal of biochemistry

authors： Otsuru O,Otsuka H,Kasama T,Seyama Y,Sakai T,Yohro T

更新日期：1983-12-01 00:00:00

abstract：:A high molecular weight protease inhibitor was purified from the egg white of Cuban crocodile (Crocodylus rhombifer). It inhibited the casein hydrolyzing activity of trypsin, subtilisin and papain. Its native molecular weight was 730,000 and it consisted of four subunits of equal molecular weight, each pair of which w...

journal_title：Journal of biochemistry

authors： Ikai A,Kitamoto T,Nishigai M

更新日期：1983-01-01 00:00:00

abstract：:We isolated and characterized an anti-idiotype monoclonal antibody (AR42.1) which is capable of mimicking a distinct and specific epitope of MUC-1 antigen. The cDNA sequences coding for the AR42.1 variable regions were determined. We found significant amino acid homology between complementary determining regions 3 (CD...

journal_title：Journal of biochemistry

authors： Luo D,Qi W,Ma J,Wang YJ,Wishart D

更新日期：2000-09-01 00:00:00

abstract：:20S proteasomes from higher eukaryotes have immunological functions rather than those from archibacteria or yeast. To clarify the mechanism of the sorting and production of antigen-presenting peptides, it is important and worthwhile to determine the structure of mammalian proteasomes using a third generation synchrotr...

journal_title：Journal of biochemistry

authors： Tomisugi Y,Unno M,Mizushima T,Morimoto Y,Tanahashi N,Tanaka K,Tsukihara T,Yasuoka N

更新日期：2000-06-01 00:00:00

abstract：:Calmodulin-dependent protein kinase IV (CaM-kinase IV) kinase was recently discovered in the rat brain by its activity to activate the inactive recombinant CaM-kinase IV expressed in Escherichia coli [Okuno, S. and Fujisawa, H. (1993) J. Biochem. 114, 167-170]. In the present study, CaM-kinase IV kinase was purified a...

journal_title：Journal of biochemistry

authors： Okuno S,Kitani T,Fujisawa H

更新日期：1994-10-01 00:00:00

abstract：:Two kinds of monoclonal antibodies (3B9 and SM1) against connectin, muscle elastic protein, reacted with both alpha- and beta-connectins. Immunofluorescence studies revealed that 3B9 stained both edges of the A band of chicken breast muscle myofibrils and remained as such upon stretching to a sarcomere length of 3.5 m...

journal_title：Journal of biochemistry

authors： Itoh Y,Suzuki T,Kimura S,Ohashi K,Higuchi H,Sawada H,Shimizu T,Shibata M,Maruyama K

更新日期：1988-10-01 00:00:00

abstract：:In order to characterize the mechanism for bilirubin transport in the liver, the uptake of bilirubin diglucuronide (BDG) into purified sinusoidal plasma membrane vesicles was investigated. BDG uptake was saturable, and was inhibited by sulfobromophthalein and unconjugated bilirubin, but was not affected by sodium taur...

journal_title：Journal of biochemistry

authors： Adachi Y,Roy-Chowdhury J,Roy-Chowdhury N,Kinne R,Tran T,Kobayashi H,Arias IM

更新日期：1990-05-01 00:00:00