Abstract:
:c-Abl non-receptor tyrosine kinase has been implicated in many cellular processes including cell differentiation, stress response and regulating gene transcription. The mechanism by which c-Abl is involved in the regulation of gene transcription remains to be elucidated. In this study, we investigated the functions of c-Abl in the activation of p21 promoter. Our results showed that overexpression of c-Abl tyrosine kinase activated p21 promoter and endogenous p21 transcription in U2OS cells. We found that p53 is involved in the activation of p21 promoter by c-Abl, and integrative structure of p53 is required for regulating p21 transcription. In addition, the chromatin immunoprecipitation study demonstrated that c-Abl and p53 can be recruited to the region containing p53 binding site of p21 promoter, and c-Abl increases the DNA binding activity of p53 to the p21 promoter. Furthermore, not only the activation of p21 promoter but also the recruitment to p21 promoter by c-Abl is dependent on the interaction between c-Abl and p53 protein.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Jing Y,Wang M,Tang W,Qi T,Gu C,Hao S,Zeng Xdoi
10.1093/jb/mvm068subject
Has Abstractpub_date
2007-05-01 00:00:00pages
621-6issue
5eissn
0021-924Xissn
1756-2651pii
mvm068journal_volume
141pub_type
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