Studies on the turnover rates of ornithine aminotransferase in Morris hepatoma 44 and host liver.

abstract：:Bovine renal brush-border membranes were solubilized by 1.6% sodium cholate. Na+/H(+)-antiporter was recovered in the supernatant after centrifugation at 160,000 x g for 1 h and was successfully reconstituted into proteoliposomes by a cholate-dialysis procedure. The reconstituted Na+/H(+)-antiporter showed a pH-gradie...

journal_title：Journal of biochemistry

authors： Yamashita T,Kawakita M

更新日期：1992-02-01 00:00:00

abstract：:Paramyosin is a myosin-binding protein characteristic of invertebrate animals, while troponin is a Ca2+-dependent regulator of muscle contraction. Both proteins are widely distributed in protostomes, while in deuterostomes, their distribution is limited; namely, presence of paramyosin and absence of troponin are commo...

journal_title：Journal of biochemistry

authors： Sonobe H,Obinata T,Minokawa T,Haruta T,Kawamura Y,Wakatsuki S,Sato N

更新日期：2016-12-01 00:00:00

abstract：:Escherichia coli RNase HII is composed of 198 amino acid residues. The enzyme has been overproduced in an insoluble form, purified in a urea-denatured form, and refolded with poor yield [M. Itaya (1990) Proc. Natl. Acad. Sci. USA 87, 8587-8591]. To facilitate the preparation of the enzyme in an amount sufficient for p...

journal_title：Journal of biochemistry

authors： Ohtani N,Haruki M,Muroya A,Morikawa M,Kanaya S

更新日期：2000-05-01 00:00:00

abstract：:The structure of a ferricytochrome c' extracted from Rhodospirillum rubrum has been determined at 6 A resolution by the X-ray crystallographic method. The crystals, obtained by dialyzing the protein solution against polyethylene glycol 4000, belong to the hexagonal space group P6(1). Two heavy atom derivatives were ob...

journal_title：Journal of biochemistry

authors： Yasui M,Harada S,Kai Y,Kasai N

更新日期：1985-07-01 00:00:00

abstract：:Kidney catalase [EC 1.11.1.6] was doubly labeled by injecting a mixture of [14C]-leucine and delta-amino[3H]levulinic acid (ALA) into normal and allyliso-(2-isopropyl-4-pentenoyl)urea (Sedormid)-treated rats. Shortly after the injection of a tracer dose or the precursors, catalase in microsomes showed the highest spec...

journal_title：Journal of biochemistry

authors： Higashi T,Kawamata F,Dogen M

更新日期：1976-08-01 00:00:00

abstract：:The patterns of myosin isozymes in embryonic and adult chicken pectoralis muscle were examined by electrophoresis in a non-denaturing gel system (pyrophosphate acrylamide gel electrophoresis), and both light chains and heavy chains of embryonic and adult myosin isozymes were compared. In pyrophosphate acrylamide gel e...

journal_title：Journal of biochemistry

authors： Takano-Ohmuro H,Obinata T,Masaki T,Mikawa T

更新日期：1982-04-01 00:00:00

abstract：:Methyl-coenzyme M reductase isoenzyme I from the methanogenic Archaeon, Methanobacterium thermoautotrophicum (strain Marburg), was crystallized by vapor diffusion methods. Crystal form M obtained with 2-methyl-2,4-pentanediol as the precipitant displayed space group P2(1), with unit cell parameters of a=83.2 A, b=117....

journal_title：Journal of biochemistry

authors： Shima S,Goubeaud M,Vinzenz D,Thauer RK,Ermler U

更新日期：1997-05-01 00:00:00

abstract：:It was found that the absorption spectrum of beta-naphthyl triphosphate is different from that of beta-naphthyl diphosphate in the range 290-335 nm. Thus, beta-naphthyl triphosphate hydrolysis by heavy meromyosin can be recorded continuously as a function of time by means of a spectrophotometer. By analyzing the time ...

journal_title：Journal of biochemistry

authors： Kuwajima T,Kagawa H,Asai H

更新日期：1975-11-01 00:00:00

abstract：:Matriptase is a type II transmembrane serine protease expressed abundantly in the epithelial cells and keratinocyte. It plays a key role in the establishment and maintenance of epithelial integrity. Matriptase is considered to be at the most upstream in cellular protease cascade. Activation of its zymogen is the most ...

journal_title：Journal of biochemistry

authors： Kojima K,Inouye K

更新日期：2011-08-01 00:00:00

abstract：:Bovine dopamine beta-monooxygenase is a tetramer consisting of two disulfide-linked dimers. To examine the role(s) of the intersubunit disulfide bonds in the protein structure and activity, the enzyme was treated with DTT at pH 7.5 and 25 degrees C under nondenaturing conditions. A 15-min incubation with 0.5 mM DTT se...

journal_title：Journal of biochemistry

authors： Ishida T,Narita M,Nozaki M,Horiike K

更新日期：1996-08-01 00:00:00

abstract：:CEL-III is a haemolytic lectin, which has two beta-trefoil domains (domains 1 and 2) and a beta-sheet-rich domain (domain 3). In domain 3 (residues 284-432), there is a hydrophobic region containing two alpha-helices (H8 and H9, residues 317-357) and a loop between them, in which alternate hydrophobic residues, especi...

journal_title：Journal of biochemistry

authors： Hisamatsu K,Tsuda N,Goda S,Hatakeyama T

更新日期：2008-01-01 00:00:00

abstract：:Cathepsin E (CE) is an intracellular aspartic proteinase that is exclusively expressed in cells of the gastrointestinal tracts, lymphoid tissues, urinary organs and red blood cells. However, the molecular mechanism by which CE is predominantly expressed in these cells remains unknown. Here, we report the identificatio...

journal_title：Journal of biochemistry

authors： Okamoto K,Okamoto Y,Kawakubo T,Iwata J,Yasuda Y,Tsukuba T,Yamamoto K

更新日期：2012-03-01 00:00:00

abstract：:Interleukin-1 (IL-1) is produced and released by various cells, including activated macrophages, and plays important roles in inflammation as well as immune responses. Since the precursor of IL-1 has no signal peptide, the mechanism of IL-1 release has been an enigma. To investigate the possibility of direct interacti...

journal_title：Journal of biochemistry

authors： Oku N,Saito N,Okada S,Watanabe N,Kobayashi Y

更新日期：1995-10-01 00:00:00

abstract：:1. Z-line constituents contributing to the formation of the contraction bands in chicken myofibrils were investigated by using the rabbit calcium-activated factor (CAF). 2. CAF digestion hampered the formation of the contraction bands and increased the amount of soluble proteins which are apparently derived from the Z...

journal_title：Journal of biochemistry

authors： Muguruma M,Muguruma Y,Fukazawa T

更新日期：1980-07-01 00:00:00

abstract：:Large amounts of extra proteins, X (in the envelope fraction) and X' (in the cytoplasmic fraction) were detected by SDS-polyacrylamide gel electrophoresis when DNA of Escherichia coli was damaged. These two proteins had the same apparent molecular weight (appproximately 40,000) and were produced under identical condit...

journal_title：Journal of biochemistry

authors： Yoda K,Nagai K,Tamura G

更新日期：1977-05-01 00:00:00

abstract：:A GTP-binding protein with an Mr of 24,000 was purified from a cholate extract of bovine brain membranes in addition to the previously reported alpha beta gamma-trimeric GTP-binding proteins (G proteins). Partial amino acid sequence analysis of the purified 24-kDa protein revealed that it was not identical to any of t...

journal_title：Journal of biochemistry

authors： Ohoka Y,Imai S,Kozasa T,Maehama T,Takahashi K,Kaziro Y,Ui M,Katada T

更新日期：1991-03-01 00:00:00

abstract：:Telomerase is an enzyme that catalyzes addition of telomeric repeat sequences to the 3'-termini of eukaryotic chromosome DNA. The catalytic core of telomerase consists of a protein component, telomerase reverse transcriptase (TERT), for the catalysis and an RNA component, telomerase RNA (TR), containing the template f...

journal_title：Journal of biochemistry

authors： Li X,Nishizuka H,Tsutsumi K,Imai Y,Kurihara Y,Uesugi S

更新日期：2007-05-01 00:00:00

abstract：:In porcine aortic smooth muscle cells, neuropeptide Y (NPY) stimulates mobilization of CA(2+) from intracellular store sites via Y1 receptors. However, it has been debated whether or not Ca(2+) mobilization by Y1 receptors depends on the generation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] following activation of...

journal_title：Journal of biochemistry

authors： Shigeri Y,Nakajima S,Fujimoto M

更新日期：1995-09-01 00:00:00

abstract：:A novel positively charged planar bilayer membrane was formed from a mixture containing 20% cationic lipid, 1,2-dioleoyloxy-3-(trimethylammonio)propane, and neutral phospholipid mixture of 56% phosphatidylethanolamine and 24% egg phosphatidylcholine. The basic properties of the bilayer were essentially the same as tho...

journal_title：Journal of biochemistry

authors： Anzai K,Masumi M,Kawasaki K,Kirino Y

更新日期：1993-10-01 00:00:00

abstract：:We purified dipeptidyl peptidase II (DPP II) to homogeneity from rat kidney and determined its physicochemical properties, including its molecular weight, substrate specificity, and partial amino acid sequence. Furthermore, we screened a rat kidney cDNA library, isolated the DPP II cDNA and determined its structure. T...

journal_title：Journal of biochemistry

authors： Araki H,Li Y,Yamamoto Y,Haneda M,Nishi K,Kikkawa R,Ohkubo I

更新日期：2001-02-01 00:00:00

abstract：:Among several intracellular protease tested, cathepsin H transformed leukotriene D4 to E4 with a release of glycine in a stoichiometric quantity. Under the optimal conditions the rate of leukotriene D4 transformation by cathepsin H was about 3% of the hydrolysis rate of alpha-N-benzoyl-DL-arginine-2-naphthylamide whic...

journal_title：Journal of biochemistry

authors： Yokota K,Shono F,Yamamoto S,Kominami E,Katunuma N

更新日期：1983-10-01 00:00:00

abstract：:The role of HSDJ, a human homolog of bacterial DnaJ and yeast YDJ1p/MAS5, in mitochondrial protein import was examined. Recombinant HSDJ was purified and an antibody was prepared. HSDJ mRNA was heat-induced in cultured cells. In pulse-labeling and chase experiments using COS-7 cells, the endogenous HSDJ homolog was pr...

journal_title：Journal of biochemistry

authors： Kanazawa M,Terada K,Kato S,Mori M

更新日期：1997-05-01 00:00:00

abstract：:The SH groups of glutamine synthetase [EC 6.3.1.2] from Bacillus stearothermophilus were modified with 5, 5'-dithiobis(2-nitrobenzoic acid) in order to determine the number of SH groups in the molecule as well as the effect of the modification on the enzyme activity. Three SH groups per subunit were detected after co...

journal_title：Journal of biochemistry

authors： Hachimori A,Takeda A,Nagaoka T,Suzuki H,Nosoh Y,Samejima T

更新日期：1975-12-01 00:00:00

abstract：:Murine interleukin-2 (MIL-2) cDNA deleted of 11 repeats of a CAG sequence, and that encoded from 33Met to 149Gln were inserted into an expression vector carrying an Escherichia coli tryptophan promoter and were expressed in E. coli, respectively. Recombinant MIL-2 deleted of 11 glutamine repeats (MIL-2(-Gln] supported...

journal_title：Journal of biochemistry

authors： Kashima N,Matsui H,Yamasaki S,Kondo N,Hamuro J

更新日期：1987-10-01 00:00:00

abstract：:By using commercially available anhydrous hydrazine in the gas-phase, mucin-type oligosaccharides were released from porcine gastric mucin (PGM) and bovine fetuin. The data indicated that a certain amount of the oligosaccharides from PGM were further degraded. Despite this, the HPLC elution profile of the anthranilic ...

journal_title：Journal of biochemistry

authors： Goso Y,Tsubokawa D,Ishihara K

更新日期：2009-06-01 00:00:00

abstract：:A new multifunctional protein kinase, which normally exists as an inactive form in the soluble fraction in mammalian tissues, attaches to membranes to exhibit full enzymatic activity. A low concentration of Ca2+ is absolutely necessary for this activation. This process is reversible. cAMP shows no effect. The active f...

journal_title：Journal of biochemistry

authors： Takai Y,Kishimoto A,Iwasa Y,Kawahara Y,Mori T,Nishizuka Y,Tamura A,Fujii T

更新日期：1979-08-01 00:00:00

abstract：:Although the depletion of reduced glutathione (GSH) has been observed in a variety of apoptotic systems, little is known about the mechanism of GSH depletion. In this study we used polarized MDCK cells to study the GSH flux during ricin-induced apoptosis. Here we report that the specific accumulation of GSH occurred i...

journal_title：Journal of biochemistry

authors： Oda T,Sadakata N,Komatsu N,Muramatsu T

更新日期：1999-10-01 00:00:00

abstract：:Myosin was isolated from leucocytes in horse arterial blood by the same procedures used for the isolation of myosin from skeletal muscle. The Ca2+-, EDTA-, and Mg2+-ATPase [EC 3.6.1.3] activities of the protein was 0.148, 0.147, and 0.001 mumoles/min/mg, respectively, in 0.5 M KCl at pH 7.0 and 25 degrees. The Ca2+-AT...

journal_title：Journal of biochemistry

authors： Takeuchi K,Shibata N,Senda N

更新日期：1975-07-01 00:00:00

abstract：:The FLI-1 transcription factor is a member of the ETS gene family, most closely related to ERG. In this study, the FLI-1 protein products were characterized using a specific monoclonal antibody previously developed against bacterially expressed protein. In the human T-cell line Jurkat, both isoforms of FLI-1, p51 and ...

journal_title：Journal of biochemistry

authors： Zhang XK,Watson DK

更新日期：2005-03-01 00:00:00

abstract：:Sialoglycoprotein which exhibits inhibitory activity for hemagglutination by Hemagglutinating Virus of Japan (HVJ, Sendai virus) was isolated from the membrane of bovine erythrocytes. Purification steps for this sialoglycoprotein included extraction with lithium diiodosalicylate, phenol partition, precipitation with e...

journal_title：Journal of biochemistry

authors： Suzuki Y,Suzuki T,Matsumoto M

更新日期：1983-06-01 00:00:00