Selective cleavage and modification of the intersubunit disulfide bonds of bovine dopamine beta-monooxygenase: conversion of tetramer to active dimer.

abstract：:The asparagine-linked sugar chains of human follicle-stimulating hormone (hFSH) were liberated as radioactive oligosaccharides from the polypeptide moiety by hydrazinolysis followed by N-acetylation and NaB3H4 reduction. Ninety-five percent of the oligosaccharides were acidic and all were converted to a mixture of neu...

journal_title：Journal of biochemistry

authors： Renwick AG,Mizuochi T,Kochibe N,Kobata A

更新日期：1987-05-01 00:00:00

abstract：:Coenzyme A (CoASH) is an obligate cofactor for lipids undergoing beta-oxidation in peroxisomes. Although the peroxisomal membrane appears to be impermeable to CoASH, peroxisomes contain their own pool of CoASH. It is believed that CoASH enters peroxisomes as acyl-CoAs, but it is not known how this pool is regulated. T...

journal_title：Journal of biochemistry

authors： Reilly SJ,Tillander V,Ofman R,Alexson SE,Hunt MC

更新日期：2008-11-01 00:00:00

abstract：:Mitochondria are dynamic organelles that frequently divide and fuse together, resulting in the formation of intracellular tubular networks. In yeast and mammals, several factors including Drp1/Dnm1 and Mfn/Fzo1 are known to regulate mitochondrial morphology by controlling membrane fission or fusion. Here, we report th...

journal_title：Journal of biochemistry

authors： Ichishita R,Tanaka K,Sugiura Y,Sayano T,Mihara K,Oka T

更新日期：2008-04-01 00:00:00

abstract：:Studies were carried out on the delta 5-desaturation reaction in ergosterol biosynthesis with a particulate fraction of cell-free extract of yeast. A reduced pyridine nucleotide coenzyme and molecular oxygen were required for the reaction. It was shown that the enzyme activity is located in a fraction corresponding to...

journal_title：Journal of biochemistry

authors： Osumi T,Nishino T,Katsuki H

更新日期：1979-03-01 00:00:00

abstract：:3-O-Methyl hexosamine was found for the first time and characterized as one of the sugar components of oyster glycolipid. The alditol acetate of this sugar was identified as 3-O-methyl-N-acetylgalactosaminitol acetate by comparing its retention time on gas chromatography and mass spectrum with those of the authentic s...

journal_title：Journal of biochemistry

authors： Matsubara T,Hayashi A

更新日期：1978-04-01 00:00:00

abstract：:When compared with collagens isolated from animal sources, collagens and collagen-like (CL) proteins from non-animal sources are non-immunogenic and thus promising as biomedical materials. Recently, a CL protein, V-CL, was identified from a bacterial source, Streptococcus pyogenes. In this study, an acid-precipitation...

journal_title：Journal of biochemistry

authors： Ma L,Chai Y,Wu T,Wang M

更新日期：2017-09-01 00:00:00

abstract：:Anti-angiotensin I IgG in serum was measured by immune complex transfer enzyme immunoassays using angiotensin I conjugates prepared by two different methods. In the first method, angiotensin I was conjugated to dinitrophenyl bovine serum albumin and beta-D-galactosidase through covalent links. Anti-angiotensin I IgG i...

journal_title：Journal of biochemistry

authors： Kohno T,Yamaguchi K,Ishikawa E

更新日期：1990-11-01 00:00:00

abstract：:A spectrofluorometric method for sensitive determination of metalloendopeptidase activity has been developed by using a bimane-peptide containing a tryptophan residue, i.e. 1,7-dioxo-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-alpha]pyrazol-3-yl-methyl- thiomethylcarbonyl-phenylalanyl-tryptophanyl-leucine (Bim-SCH2CO-Phe-Trp-L...

journal_title：Journal of biochemistry

authors： Kajiwara K,Kumazaki T,Sato E,Kanaoka Y,Ishii S

更新日期：1991-09-01 00:00:00

abstract：:The mitochondrial regulatory region (mrr) located between the tRNAPhe and tRNAPro genes of mitochondrial DNA (mtDNA) is essential for regulation of replication and transcription of the mitochondrial genome. Polyadenylated short RNAs complementary to the L-strand of the mrr in human cells and similar RNAs (polyadenylat...

journal_title：Journal of biochemistry

authors： Nakamichi N,Rhoads DD,Hayashi JI,Kagawa Y,Matsumura T

更新日期：1998-03-01 00:00:00

abstract：:The gizzard tropomyosin molecule is composed of two subunits at 1:1 molar ratio. Possible composites of the tropomyosin molecule are two kinds of homodimer (one for each subunit), a heterodimer of two subunits, or a mixture of heterodimer and homodimer(s). We tried to evaluate the native subunit composition of gizzard...

journal_title：Journal of biochemistry

authors： Nakamura S,Sakurai T,Nonomura Y

更新日期：1991-05-01 00:00:00

abstract：:An actin-depolymerizing marine natural product, mycalolide B, and a related compound, kabiramide D, were labeled with biocytin, a biotin derivative, and used to specify target molecules in cultured rat 3Y1 fibroblasts. Mycalolide B exhibited the ability to bind to various intracellular proteins, probably through the M...

journal_title：Journal of biochemistry

authors： Wada S,Matsunaga S,Saito S,Fusetani N,Watabe S

更新日期：1998-05-01 00:00:00

abstract：:In this paper we speculate on the origin and early evolution of transition element enzymes. Iron, molybdenum, and zinc, the most abundant transition elements in seawater, presumably complexed with compounds accumulated in the primeval sea in the course of chemical evolution forming compounds with subsequently evolved ...

journal_title：Journal of biochemistry

authors： Egami F

更新日期：1975-06-01 00:00:00

abstract：:Cytolethal distending toxin (CDT) is one of the exotoxins produced by Actinobacillus actinomycetemcomitans, an agent of localized aggressive periodontitis. We constructed N-terminal deletion mutants of CdtA using an Escherichia coli expression system and found that ADelta19-47, with a deletion from Asn-19 to Pro-47, s...

journal_title：Journal of biochemistry

authors： Saiki K,Gomi T,Konishi K

更新日期：2004-09-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase (PEPC) [EC 4.1.1.31] has a highly conserved and unique sequence, 578-FHGRGGSIGRGGAP-591 (on Escherichia coli, PEPC), in which a GRGG motif is repeated twice with two intervening residues. Since previous chemical modification studies suggested the functional importance of arginine residu...

journal_title：Journal of biochemistry

authors： Yano M,Terada K,Umiji K,Izui K

更新日期：1995-06-01 00:00:00

abstract：:The relationship between structure, activity, and stability of the thermostable Bacillus stearothermophilus alpha-amylase was studied by site-directed mutagenesis of the three most conserved residues. Mutation of His-238 to Asp involved in Ca2+ and substrate binding reduced the specific activity and thermal stability,...

journal_title：Journal of biochemistry

authors： Vihinen M,Ollikka P,Niskanen J,Meyer P,Suominen I,Karp M,Holm L,Knowles J,Mäntsälä P

更新日期：1990-02-01 00:00:00

abstract：:The complete amino acid sequence of ribonuclease N1 (RNase N1), a guanine-specific ribonuclease from a fungus, Neurospora crassa, was determined by conventional protein sequencing, using peptide fragments obtained by tryptic digestion of cyanogen bromide-treated RNase N1 and by Staphylococcus aureus V8 protease digest...

journal_title：Journal of biochemistry

authors： Takahashi K

更新日期：1988-09-01 00:00:00

abstract：:Carboxypeptidase Y (CPY) inhibitor, I(C), a cytoplasmic inhibitor of vacuolar proteinases in yeast, Saccharomyces cerevisiae, was purified by means of a high-level expression system using a proteinase-deficient strain, BJ2168, and an expression vector with the promoter GAL1. The purified I(C) exists as a monomeric bet...

journal_title：Journal of biochemistry

authors： Mima J,Suzuki H,Takahashi M,Hayashi R

更新日期：2002-12-01 00:00:00

abstract：:We have previously reported that the amount of Apg-2, an Hsp110 family protein, decreases during apoptosis in Jurkat T cells. Since we hypothesized that Apg-2 would be cleaved by caspase-3 during apoptosis, a cleavage-site-directed antibody was raised against the carboxyl-terminus of the Apg-2 fragment that appears af...

journal_title：Journal of biochemistry

authors： Kato M,Nonaka T,Imajoh-Ohmi S

更新日期：2005-06-01 00:00:00

abstract：:Pork insulin was subjected to mercaptosuccinylation and then coupled to beta-D-galactosidase [EC 3.2.1.23] from Escherichia coli using N,N'-o-phenylenedimaleimide. The competitive binding of the conjugate and insulin to anti-insulin antibody was tested. Results showed that formation of an insulin-beta-D-galactosidase ...

journal_title：Journal of biochemistry

authors： Kato K,Hamaguchi Y,Fukui H,Ishikawa E

更新日期：1975-07-01 00:00:00

abstract：:The effects of various compounds on ascorbate-2-sulfate sulfohydrolase and arylsulfatase (EC 3.1.6.1) activities in the copurified preparation from the liver of Charonia lampas were investigated. The former activity was competively inhibited by inorganic phosphate and sulfate. The latter was not affected by sulfate....

journal_title：Journal of biochemistry

authors： Hatanaka H,Ogawa Y,Egami F

更新日期：1975-04-01 00:00:00

abstract：:Sodium- and potassium-activated adenosinetriphosphatase (Na+, K+-ATPase) purified from dog kidney outer medulla was examined by polyacrylamide gel electrophoresis and by photoaffinity labeling with N-(ouabain)-N'-(2-nitro-4-azidophenyl)-ethylenediamine (NAP-ouabain). The large subunit band (alpha-band) split into two ...

journal_title：Journal of biochemistry

authors： Ohta T,Kawamura M,Hasegawa T,Ishikura H,Nagano K

更新日期：1982-01-01 00:00:00

abstract：:The Escherichia coli cytochrome bo is a heme-copper terminal ubiquinol oxidase, and functions as a redox-driven proton pump. We applied pulse radiolysis technique for studying the one-electron reduction processes in the Cu(B)-deficient mutant, His333Ala. We found that the Cu(B) deficiency suppressed the heme b-to-heme...

journal_title：Journal of biochemistry

authors： Kobayashi K,Tagawa S,Mogi T

更新日期：2009-05-01 00:00:00

abstract：:Type A atrial natriuretic peptide (ANP) receptor was demonstrated to be present as a tetramer in the bovine adrenal cortex. Type A ANP receptor is composed of two functional domains, namely extracellular ANP-binding and cytoplasmic guanylate cyclase domains, and generally considered to be present as a single polypepti...

journal_title：Journal of biochemistry

authors： Iwata T,Uchida-Mizuno K,Katafuchi T,Ito T,Hagiwara H,Hirose S

更新日期：1991-07-01 00:00:00

abstract：:The denaturation of recombinant horse L-chain apoferritin (rLF), which is composed of 24 L-chain subunits, in acidic solution was studied. Using two rLF mutants, lacking four (Fer4) or eight (Fer8) N-terminal amino acid residues, the effect of N-terminal residues on the protein's stability was investigated. Of the two...

journal_title：Journal of biochemistry

authors： Yoshizawa K,Mishima Y,Park SY,Heddle JG,Tame JR,Iwahori K,Kobayashi M,Yamashita I

更新日期：2007-12-01 00:00:00

abstract：:Adult rat hepatocytes in primary culture cannot survive more than 2 days in the absence of calf serum. An extract of bovine pituitary gland had a similar effect to calf serum on the survival of hepatocytes, but its specific activity was 70 times that of calf serum. The survival factor was purified from bovine pituitar...

journal_title：Journal of biochemistry

authors： Asami O,Nakamura T,Mura T,Ichihara A

更新日期：1984-02-01 00:00:00

abstract：:Glycosphingolipids (GSLs), cell type-specific markers which change dramatically during ontogenesis and oncogenesis, have been implicated as playing major roles in cellular interactions and control of cell proliferation in multicellular organisms. These functional roles have been partially clarified through two types o...

journal_title：Journal of biochemistry

authors： Hakomori S,Igarashi Y

更新日期：1995-12-01 00:00:00

abstract：:The mitochondrion is an essential organelle for a wide range of cellular processes, including energy production, metabolism, signal transduction and cell death. To execute these functions, mitochondria regulate their size, number, morphology and distribution in cells via mitochondrial division and fusion. In addition,...

journal_title：Journal of biochemistry

authors： Murata D,Arai K,Iijima M,Sesaki H

更新日期：2020-03-01 00:00:00

abstract：:We purified dipeptidyl peptidase II (DPP II) to homogeneity from rat kidney and determined its physicochemical properties, including its molecular weight, substrate specificity, and partial amino acid sequence. Furthermore, we screened a rat kidney cDNA library, isolated the DPP II cDNA and determined its structure. T...

journal_title：Journal of biochemistry

authors： Araki H,Li Y,Yamamoto Y,Haneda M,Nishi K,Kikkawa R,Ohkubo I

更新日期：2001-02-01 00:00:00

abstract：:The heparin-induced self-aggregation behaviours of four repeat peptides (R1-R4) in an acidic solution (pH = 4.5) were investigated by fluorescence and circular dichroism (CD) measurements and compared with those in a neutral solution (pH = 7.5). In contrast with the self-aggregation-resistive behaviours of the R1 and ...

journal_title：Journal of biochemistry

authors： Mizushima F,Minoura K,Tomoo K,Sumida M,Taniguchi T,Ishida T

更新日期：2007-07-01 00:00:00

abstract：:Protein carbonyls are reported to increase in aging and in pathologies such as Alzheimer's disease and ischemic injury. Detailed study of this important issue has, however, been hampered by lack of an appropriate method to identify individual carbonylated proteins. We describe here an immunoblot method to identify ind...

journal_title：Journal of biochemistry

authors： Nakamura A,Goto S

更新日期：1996-04-01 00:00:00