Abstract:
:Anti-angiotensin I IgG in serum was measured by immune complex transfer enzyme immunoassays using angiotensin I conjugates prepared by two different methods. In the first method, angiotensin I was conjugated to dinitrophenyl bovine serum albumin and beta-D-galactosidase through covalent links. Anti-angiotensin I IgG in rabbit serum was reacted simultaneously with dinitrophenyl bovine serum albumin-angiotensin I conjugate and beta-D-galactosidase-angiotensin I conjugate, and the complex formed of the three components was trapped onto (anti-dinitrophenyl group) IgG-coated polystyrene balls. After washing, the complex was eluted from the polystyrene balls with dinitrophenyl-L-lysine and transferred to goat (anti-rabbit IgG) IgG-coated polystyrene balls. Beta-D-Galactosidase activity bound to (anti-rabbit IgG) IgG-coated polystyrene balls was assayed by fluorometry. In the second method, biotinylated angiotensin I was coupled with dinitrophenyl bovine serum albumin-avidin conjugate and Beta-D-galactosidase-avidin conjugate and substituted for the two conjugates in the first method. The detection limits of anti-angiotensin I IgG in serum were 10-30 ng/liter (0.2-0.6 pg/assay). These methods were 330 to 1,000-fold more sensitive and much less affected by serum effect than the conventional enzyme immunoassay, in which an angiotensin I-bovine serum albumin-coated polystyrene ball was incubated with anti-angiotensin I IgG in serum and, after washing, with (anti-rabbit IgG) Fab'-peroxidase conjugate. The first method was more sensitive than the second method, but the second method may be superior in applicability to the first method.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Kohno T,Yamaguchi K,Ishikawa Edoi
10.1093/oxfordjournals.jbchem.a123275subject
Has Abstractpub_date
1990-11-01 00:00:00pages
741-7issue
5eissn
0021-924Xissn
1756-2651journal_volume
108pub_type
杂志文章abstract::Oxidative activity of 9-anthraldehyde (9-AA) to 9-anthracenecarboxylic acid in monkey liver was mainly located in microsomes. The reaction required NADPH as an essential cofactor and was significantly inhibited by SKF 525-A, metyrapone, disulfiram, and CO, potent inhibitors of microsomal aldehyde oxygenase (MALDO). Tw...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1996-04-01 00:00:00
abstract::ISG15 is a ubiquitin-like protein that is upregulated on treatment with interferon. ISG15 is considered to be covalently conjugated to cellular proteins through a sequential reaction similar to that of the ubiquitin conjugation system consisting of E1/E2/E3 enzymes: UBE1L and UbcH8 have been reported to function as E1...
journal_title:Journal of biochemistry
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更新日期:1996-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1996-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1975-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135196
更新日期:1985-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133653
更新日期:1981-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122334
更新日期:1988-04-01 00:00:00
abstract::Formate dehydrogenase (EC 1.2.1.2) prepared from peas (Pisum sativum) was a two-subunit enzyme. The enzyme accelerated the formation of an NAD+-cyanide compound having an adsorption band at 330 nm. The enzyme was able to bind one NAD+ molecule per each subunit but only 1 mole of NAD+-cyanide compound was formed per ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a130792
更新日期:1975-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2001-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1998-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a022204
更新日期:1998-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132683
更新日期:1979-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2009-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1982-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
abstract::The nucleotide sequence of the mRNA for NADPH-cytochrome P-450 reductase from rabbit liver was determined from a full-length cDNA clone (pFP105). The clone contains 2,269 nucleotides complementary to rabbit liver reductase mRNA. The single open reading frame of 2,037 nucleotides codes for a 679-amino acid polypeptide ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a121807
更新日期:1986-10-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1980-12-01 00:00:00
abstract::Four flavonoids (i.e., baicalein, quercetin, quercetagetin, and myricetin), known to be inhibitors of HIV-reverse transcriptase, have been shown to be more or less inhibitory to the activities of various cellular DNA and RNA polymerases. The degree of the inhibition varied depending on the combination of the flavonoid...
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pub_type: 杂志文章
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更新日期:1990-10-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:
更新日期:1979-05-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1978-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131648
更新日期:1977-06-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1991-09-01 00:00:00