Evaluation of conditions for release of mucin-type oligosaccharides from glycoproteins by hydrazine gas treatment.

abstract：:A sugar autoanalyzer was used on a preparative scale to resolve a gluco-oligosaccharide mixture. In this way the components of the following mixtures were resolved: O-alpha-D-glucopyranosyl-(1-3)-O-[alpha-D-glucopyranosyl-(1-6)]-D-glucose (1), O-alpha-D-glucopyranosyl-(1-6)-O-alpha-D-glucopyranosyl-(1-3)-D-glucose (2)...

journal_title：Journal of biochemistry

authors： Torii M,Alberto BP,Tanaka S,Tsumuraya Y,Misaki A,Sawai T

更新日期：1979-03-01 00:00:00

abstract：:The allosteric properties of AMP deaminase [EC 3.5.4.6] from chicken erythrocytes have been qualitatively and quantitatively accounted for by the concerted transition theory of Monod et al., on the assumption that this enzyme has different numbers of binding sites for each ligand. Theoretical curves yield a satisfacto...

journal_title：Journal of biochemistry

authors： Yoshino M,Kawamura Y,Ogasawara N

更新日期：1976-08-01 00:00:00

abstract：:It has been demonstrated that the GM2 ganglioside cannot be cleaved by exo-beta-N-acetylhexosaminidases isolated from molds or bacterial sources. Here, a novel GM2 ganglioside-degrading enzyme was found in cells of Nocardia sp. This enzyme releases free fatty acids from the GM2 ganglioside. The chemical structure of t...

journal_title：Journal of biochemistry

authors： Hirabayashi Y,Kimura M,Matsumoto M,Yamamoto K,Kadowaki S,Tochikura T

更新日期：1988-01-01 00:00:00

abstract：:Cilia and flagella of eukaryotic cells are evolutionarily conserved organelles with a microtubule-based axoneme as a scaffold. To fulfil their functions in cellular motility, sensory reception and developmental signalling, cilia contain unique proteins, such as receptors and ion channels. The assembly and maintenance ...

journal_title：Journal of biochemistry

authors： Nakayama K,Katoh Y

更新日期：2018-03-01 00:00:00

abstract：:The Escherichia coli strain carrying pTP 6-10 which was constructed in our previous work (Iwakura, M., et al. (1983) J. Biochem. 93, 927-930) produces more than 400-fold dihydrofolate reductase as compared with the strain without the plasmid. Dihydrofolate reductase was highly purified from the cell-free extract of th...

journal_title：Journal of biochemistry

authors： Iwakura M,Shimura Y,Sakai T,Tsuda K

更新日期：1983-09-01 00:00:00

abstract：:LipB, the lipase activator protein of Pseudomonas aeruginosa TE3285, was overproduced in Escherichia coli, and purified 4.9-fold over the crude extract in the presence of SDS. The purified LipB reactivated the lipase from P. aeruginosa TE3285 denatured with guanidine hydrochloride, and its reactivation did not involve...

journal_title：Journal of biochemistry

authors： Shibata H,Kato H,Oda J

更新日期：1998-01-01 00:00:00

abstract：:Serum-free culture medium collected from primary monolayer cultures of human articular chondrocytes was found to inhibit human urokinase [EC 3.4.21.31] activity. Although chondrocyte culture medium contained a small amount of endothelial-type plasminogen activator inhibitor which could be demonstrated by reverse fibri...

journal_title：Journal of biochemistry

authors： Yamada H,Stephens RW,Nakagawa T,McNicol D

更新日期：1988-12-01 00:00:00

abstract：:F-Actin was digested with alpha-chymotrypsin in 6 M urea, and two peptide fragments from subdomain 4 of actin molecule [Kabsch, W., Mannherz, H.G., Suck, D., Pai, E.F., & Holmes K.C. (1990) Nature 347, 37-44] were purified by reverse-phase HPLC and Sephadex G-50 gel filtration. The peptide fragments were identified as...

journal_title：Journal of biochemistry

authors： Hori K,Morita F

更新日期：1992-09-01 00:00:00

abstract：:1. The endogenous phosphorylation of mouse brain microsomes was studied using the technique of acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). 2. It was found that specific proteins and lipids in brain microsomes were phosphorylated by the terminal phosphate of ATP under appropriate con...

journal_title：Journal of biochemistry

authors： Satomi D

更新日期：1977-12-01 00:00:00

abstract：:To investigate a possible function of plasma albumin in partitioning organic anions into bile and urine, phenolsulfonphthalein (PSP) was administered intravenously and its in vivo fate was studied in normal and analbuminemic mutant rats (NAR). No significant change in the rate of PSP disappearance was observed in bila...

journal_title：Journal of biochemistry

authors： Inoue M,Koyama H,Nagase S,Morino Y

更新日期：1986-07-01 00:00:00

abstract：:Two distinct ferredoxins were purified from Rhodobacter capsulatus SB1003. Their complete amino acid sequences were determined by a combination of protease digestion, BrCN cleavage and Edman degradation. Ferredoxins I and II were composed of 64 and 111 amino acids, respectively, with molecular weights of 6,728 and 12,...

journal_title：Journal of biochemistry

authors： Saeki K,Suetsugu Y,Yao Y,Horio T,Marrs BL,Matsubara H

更新日期：1990-09-01 00:00:00

abstract：:We previously showed that fatty liver was easily induced in suncus by starvation and that the plasma level of apolipoprotein B (apo B) was very low. There are three possible explanations for the low level of apo B in the animals: low synthetic rate, low secretion rate, and rapid catabolism in the circulation of apo B....

journal_title：Journal of biochemistry

authors： Nagayoshi A,Matsuki N,Saito H,Tsukamoto K,Kaneko K,Wakashima M,Kinoshita M,Yamanaka M,Teramoto T

更新日期：1995-04-01 00:00:00

abstract：:A GTP-binding protein with an Mr of 24,000 was purified from a cholate extract of bovine brain membranes in addition to the previously reported alpha beta gamma-trimeric GTP-binding proteins (G proteins). Partial amino acid sequence analysis of the purified 24-kDa protein revealed that it was not identical to any of t...

journal_title：Journal of biochemistry

authors： Ohoka Y,Imai S,Kozasa T,Maehama T,Takahashi K,Kaziro Y,Ui M,Katada T

更新日期：1991-03-01 00:00:00

abstract：:We have detected in rat liver cytosol three enzymes (termed C-1, C-2, and C-3) which cleaved the RNA moiety of RNA-DNA hybrid. These enzymes were separated from each other by DEAE-Sephadex and Sephadex G-200 chromatography. C-1 and C-2 specifically act on the RNA moiety of RNA-DNA hybrid, while C-3 degrades single-str...

journal_title：Journal of biochemistry

authors： Tashiro F,Ueno Y

更新日期：1978-08-01 00:00:00

abstract：:The Ca2+-sensitive ATPase activity of rabbit skeletal myofibrils disappeared completely after treatment with a solution containing CDTA, a strong divalent cation chelator, at a low ionic strength. A gel electrophoretic study revealed that all troponin C and about half of myosin light chain 2 were removed from the myof...

journal_title：Journal of biochemistry

authors： Morimoto S,Ohtsuki I

更新日期：1987-02-01 00:00:00

abstract：:Glycogen phosphorylase (GP) is biologically active as a dimer of identical subunits. Each subunit has two distinct maltooligosaccharide binding sites: a storage site and a catalytic site. Our characterization of the properties of these sites suggested that GP activity consists of two activities: (i) binding to the gly...

journal_title：Journal of biochemistry

authors： Makino Y,Fujii Y,Taniguchi M

更新日期：2015-06-01 00:00:00

abstract：:Specific palindromic sequences in synthetic oligonucleotides are required to induce IFN and augment IFN-mediated natural killer activity. To study the mechanism of IFN induction by oligonucleotides containing palindromic sequences, we investigated the possible target molecules of the oligonucleotides. Oligo-1, a 30mer...

journal_title：Journal of biochemistry

authors： Kimura Y,Sonehara K,Kuramoto E,Makino T,Yamamoto S,Yamamoto T,Kataoka T,Tokunaga T

更新日期：1994-11-01 00:00:00

abstract：:Controlled chymotryptic digestion of the 165,000 dalton component of the M-line of rabbit skeletal muscle, followed by Biogel P-150 chromatography of the digest, has led to the isolation of a homogeneous 100,000 dalton species. This fragment was found, by both sedimentation equilibrium and gel filtration chromatograph...

journal_title：Journal of biochemistry

authors： Mani RS,Kay CM

更新日期：1979-12-01 00:00:00

abstract：:Despite the usefulness of the patch-clamp technique, its application to ion pumps and transporters in biomembranes is limited. We developed a novel method for determining the activity of a proton-pumping pyrophosphatase (H(+)-PPase) made of a single protein. We heterologously highly expressed the enzyme in Saccharomyc...

journal_title：Journal of biochemistry

authors： Nakanishi Y,Yabe I,Maeshima M

更新日期：2003-10-01 00:00:00

abstract：:The changes in the cellular concentrations of ATP, ADP, and AMP and in oxidative phosphorylation of mitochondria were investigated in the remaining liver of partially hepatectomized rabbits. The energy charge (defined as half of the average number of anhydride-bonded phosphate groups per adenosine moiety) of the liver...

journal_title：Journal of biochemistry

authors： Kamiyama Y,Ozawa K,Honjo I

更新日期：1976-10-01 00:00:00

abstract：:d-Aspartate is found in the nervous and reproductive system and participates in various physiological roles. While several lines of evidence suggest that this amino acid has an endogenous origin, the enzyme responsible for mammalian d-Asp biosynthesis has not yet been identified. We show that mammalian serine racemase...

journal_title：Journal of biochemistry

authors： Ito T,Hayashida M,Kobayashi S,Muto N,Hayashi A,Yoshimura T,Mori H

更新日期：2016-12-01 00:00:00

abstract：:Acetohydroxy acid synthetase [EC 4.1.3.18] of Neurospora crassa has been solubilized from a mitochondrial pellet fraction by sonic treatment in 1.0 M potassium phosphate buffer at pH 7.5 containing 10 mM MgSO4 and centrifugation at 180,000 X g for 20 min. The soluble enzyme thus obtained has a high specific activity a...

journal_title：Journal of biochemistry

authors： Kuwana H,Date M

更新日期：1975-01-01 00:00:00

abstract：:Daunomycin, an anti-neoplastic agent, is known to be sequestered by acidic organelles in normal and multidrug-resistant cells [Willingham, M.C., Cornwell, M.M., Cardarelli, C.O., Gottesman, M.M., & Pastan, I. (1986) Cancer Res. 46, 5941-5946]. We studied the mechanism of accumulation of daunomycin into acidic organell...

journal_title：Journal of biochemistry

authors： Moriyama Y,Manabe T,Yoshimori T,Tashiro Y,Futai M

更新日期：1994-02-01 00:00:00

abstract：:GM2 containing NeuGc was found to be a major ganglioside in the liver of most inbred strains of mouse we have examined so far. They were DBA/2, C3H/He, C57BL/6, BALB/c, and CBA mice. However, WHT/Ht mouse does not contain a detectable amount of GM2 (NeuGc) but has GM3 containing NeuGc as the major ganglioside in the l...

journal_title：Journal of biochemistry

authors： Hashimoto Y,Otsuka H,Sudo K,Suzuki K,Suzuki A,Yamakawa T

更新日期：1983-03-01 00:00:00

abstract：:The reactions of diol dehydratase with 3-unsaturated 1,2-diols and thioglycerol were investigated. Holodiol dehydratase underwent rapid and irreversible inactivation by either 3-butene-1,2-diol, 3-butyne-1,2-diol or thioglycerol without catalytic turnovers. In the inactivation, the Co-C bond of adenosylcobalamin under...

journal_title：Journal of biochemistry

authors： Toraya T,Tamura N,Watanabe T,Yamanishi M,Hieda N,Mori K

更新日期：2008-10-01 00:00:00

abstract：:We have studied the protein composition of the pectoralis superficialis muscle of genetically dystrophic (New Hampshire line 413) and normal control (line 412) chickens by one- and two-dimensional gel electrophoresis. A protein, referred to hereafter as the 30 kDa abnormal protein, was specifically detected in the aff...

journal_title：Journal of biochemistry

authors： Kobayashi R,Toyoshima I,Masamune O,Tashima Y

更新日期：1990-01-01 00:00:00

abstract：:The G72 gene is one of the most susceptible genes to schizophrenia and is contained exclusively in the genomes of primates. The product of the G72 gene modulates the activity of D-amino acid oxidase (DAO) and is a small protein prone to aggregate, which hampers its structural studies. In addition, lack of a known stru...

journal_title：Journal of biochemistry

authors： Kato Y,Fukui K

更新日期：2017-02-01 00:00:00

abstract：:Cytokine-induced neutrophil chemoattractant (CINC/Gro) is a chemotactic factor for neutrophils in the rat and a member of the CXC chemokine family. The refined three-dimensional structure of CINC/Gro was derived with the aid of heteronuclear magnetic resonance spectroscopy and a hybrid method of distance geometry and ...

journal_title：Journal of biochemistry

authors： Hanzawa H,Haruyama H,Konishi K,Watanabe K,Tsurufuji S

更新日期：1998-01-01 00:00:00

abstract：:A transition-state analog, gluconolactone, was found to partially quench the protein fluorescence of glucoamylase [EC 3.2.1.3] from Rhizopus niveus. The interaction between gluconolactone and the enzyme was studied statically and kinetically at pH 4.5 in terms of fluorescence change. The dissociation constant Kd of th...

journal_title：Journal of biochemistry

authors： Ohnishi M,Yamashita T,Hiromi K

更新日期：1977-01-01 00:00:00

abstract：:Two cultured cell lines, called Kan-R1 and Kan-R2, were established from rat hepatic cells by in vitro culture with a hepatocarcinogen, 3-methoxy-4-aminoazobenzene, and examined for the gene expression of cytochrome p450 (p450) isoforms, CYP1A1, CYP1A2, CYP2B1, CYP2B2, CYP3A1 and CYP3A2, by the RT-PCR method. It was r...

journal_title：Journal of biochemistry

authors： Degawa M,Namiki M,Yoshimoto N,Makino M,Iwamoto M,Nemoto K,Hashimoto Y

更新日期：2003-06-01 00:00:00