Serine racemase is involved in d-aspartate biosynthesis.

abstract：:The 5'-AMP-activated protein kinase (AMPK) functions as a cellular energy sensor. 5-Aminoimidazole-4-carboxyamide-1-β-D-ribofranoside (AICAR) is a chemical activator of AMPK. In the liver, AICAR suppresses expression of thephosphoenolpyruvate carboxykinase(PEPCK) gene. The rat enhancer of split- and hairy-related prot...

journal_title：Journal of biochemistry

authors： Komatsu Y,Yanagisawa Y,Moriizumi M,Tsuchiya Y,Yokouchi H,Otsuka H,Aoyagi M,Tsukada A,Kanai Y,Haneishi A,Takagi K,Asano K,Ono M,Tanaka T,Tomita K,Yamada K

更新日期：2016-04-01 00:00:00

abstract：:Polynucleotide phosphorylase from Thermus thermophilus catalyzed the addition of short guanylyl blocks from GDP to the 3'-hydroxyl termini of oligonucleotide primers at low temperature in a simple reaction mixture. Polyguanylic acid formation was inhibited at 37 degrees C, but the addition of one or two guanylyl resid...

journal_title：Journal of biochemistry

authors： Kikuchi Y,Hirai K,Sakaguchi K

更新日期：1979-11-01 00:00:00

abstract：:A chromosomal gene, mlr6793, in Mesorhizobium loti was identified as the gene encoding 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid (FHMPC) dehydrogenase (dismutase) involved in the degradation pathway for pyridoxine (vitamin B(6)). The homogenously purified recombinant enzyme has a molecular mass of 59.1 kDa...

journal_title：Journal of biochemistry

authors： Yokochi N,Yoshikane Y,Matsumoto S,Fujisawa M,Ohnishi K,Yagi T

更新日期：2009-04-01 00:00:00

abstract：:Human platelet membrane glycoprotein IV (GPIV), one of the major glycoprotein components, was purified by successive affinity chromatographies on columns of Lens culinaris agglutinin-Sepharose and wheat germ agglutinin-Sepharose followed by preparative polyacrylamide gel electrophoresis in the presence of sodium dodec...

journal_title：Journal of biochemistry

authors： Tsuji T,Osawa T

更新日期：1986-10-01 00:00:00

abstract：:The prothrombin-converting activity of Factor Xa was enhanced by thrombin-stimulated Factor V-deficient platelets and supplementary extraneous Factor Va, and also by thrombin-stimulated normal human platelets. Both extraneous Factor Va and intra-platelet Factor Va were equally inactivated by a gamma-carboxyglutamic ac...

journal_title：Journal of biochemistry

authors： Suzuki K,Nishioka J,Matsuda M,Murayama H,Hashimoto S

更新日期：1984-08-01 00:00:00

abstract：:Transient and steady state kinetics were studied in the interactions of ATP with acto-H-meromyosin reconstituted from bovine arterial heavy-meromyosin (HMM) and rabbit skeletal muscle F-actin. The results showed that the rate of dissociation of the hybrid acto-HMM induced by ATP was slower than the rate of the fluores...

journal_title：Journal of biochemistry

authors： Takeuchi K,Tonomura Y

更新日期：1978-08-01 00:00:00

abstract：:Three alkaline DNases, A, B, and C, with preference for the digestion of double-stranded DNA (dsDNA) were partially purified from microplasmodia of Physarum polycephalum. They were very similar but differed in their isoelectric points. These were pH 5.8 for DNase A, 7.1 for DNase B, and 9.1 for DNase C. All three enzy...

journal_title：Journal of biochemistry

authors： Waterborg JH,Kuyper CM

更新日期：1980-02-01 00:00:00

abstract：:Previously, we demonstrated that the 2.6-kDa peptide extending from Arg177 to Tyr198 in subdomain 4 of rabbit skeletal actin bound to actin itself, inhibited the elongation of actin filament, and severed F-actin. The corresponding segment in actin, therefore, is thought to contain the most critical actin-actin contact...

journal_title：Journal of biochemistry

authors： Hori K,Morita F,Matsuzawa F,Aikawa S

更新日期：1995-12-01 00:00:00

abstract：:I-protein was mixed with myosin before or after myosin filaments were reconstituted. In both cases, I-protein seemed to accelerate the myosin assembly. The binding of I-protein to myosin filaments was tested by sedimentation experiments and SDS-polyacrylamide gel electrophoresis. In a low ionic strength solution at pH...

journal_title：Journal of biochemistry

authors： Ohashi K,Ishikawa K,Maruyama K

更新日期：1989-07-01 00:00:00

abstract：:We previously reported that DNAs directly applicable to restriction analyses and transformation of Escherichia coli can be extracted from fungi and yeasts by use of anhydrous hydrogen fluoride (HF) under a mild condition, 5 min at 0 degrees C [Oshiro, S., Katsura, N., Kitada, K., and Gunge, N. (1987) FEBS Lett. 220, 3...

journal_title：Journal of biochemistry

authors： Oshiro S,Katsura N

更新日期：1995-03-01 00:00:00

abstract：:Rhodopsin from the bovine rod outer segment contains a covalently linked carbohydrate moiety (Heller, J. & Lawrence, M.A. (1973) Biochemistry 9, 864--868). We studied the location of this carbohydrate moiety on the disk membrane by using ferritin-conjugated concanavalin A and concanavalin A labelled with fluorescein i...

journal_title：Journal of biochemistry

authors： Nakano T,Ikai A,Nishigai M,Noda H

更新日期：1979-05-01 00:00:00

abstract：:The electron transport system coupled to the oxidation of methylamine in Pseudomonas AM1 was investigated by reconstituting it from the highly purified components. A mixture of methylamine dehydrogenase, cytochrome cH and cytochrome c oxidase (= cytochrome aa3) actively oxidized methylamine (161 mol of O2 consumed/mol...

journal_title：Journal of biochemistry

authors： Fukumori Y,Yamanaka T

更新日期：1987-02-01 00:00:00

abstract：:Proton translocating ATPase (F0F1) from bovine heart mitochondria was reconstituted into planar phospholipid bilayers, and its electrogenicity was directly demonstrated. The F0F1 ATPase was solubilized using 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonic acid (CHAPS) as a detergent followed by sucrose densi...

journal_title：Journal of biochemistry

authors： Muneyuki E,Ohno K,Kagawa Y,Hirata H

更新日期：1987-12-01 00:00:00

abstract：:We previously reported that caldesmon (CaD), together with tropomyosin (TM), effectively protects actin filaments from gelsolin, an actin-severing protein. To elucidate the structure/function relationship of CaD, we dissected the functional domain of CaD required for the protection. The basic C-terminal half of rat no...

journal_title：Journal of biochemistry

authors： Takiguchi K,Matsumura F

更新日期：2005-12-01 00:00:00

abstract：:N-acylethanolamines (NAEs) are a class of bioactive lipid molecules in animal tissues, including the endocannabinoid anandamide and the anti-inflammatory substance N-palmitoylethanolamine. Enzymatic hydrolysis of NAEs is considered to be an important step to regulate their endogenous levels. Lysosomal NAE-hydrolysing ...

journal_title：Journal of biochemistry

authors： Wang J,Zhao LY,Uyama T,Tsuboi K,Wu XX,Kakehi Y,Ueda N

更新日期：2008-11-01 00:00:00

abstract：:To investigate the role of carboxyl groups of glucoamylases [EC 3.2.1.3] from a Rhizopus sp. (Gluc1 and Gluc2), the modification of Gluc1 and Gluc2 with a water-soluble carbodiimide, 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl)carbodiimide metho-p-toluenesulfonate (CMC), was studied. The inactivation of Gluc1 proceeded wi...

journal_title：Journal of biochemistry

authors： Iwama M,Ohtsuki R,Takahashi T,Irie M

更新日期：1984-08-01 00:00:00

abstract：:Midkine (MK) is a 13-kDa heparin-binding growth/differentiation factor, and its interaction with heparan sulfate proteoglycan is important in promotion of neurite outgrowth. This study was performed to reveal the species of syndecans that interact with MK during embryogenesis of the central nervous system in the rat. ...

journal_title：Journal of biochemistry

authors： Nakanishi T,Kadomatsu K,Okamoto T,Ichihara-Tanaka K,Kojima T,Saito H,Tomoda Y,Muramatsu T

更新日期：1997-02-01 00:00:00

abstract：:The conditions for the preparation of the ferricytochrome a-ferrocytochrome a3-carbon monoxide complex (a3+, a3(2)+CO) of cytochrome oxidase [EC 1.9.3.1] by the ferricyanide-reoxidation method and some properties of the prepared complex were studied. The addition of a small volume of concentrated ferricyanide solution...

journal_title：Journal of biochemistry

authors： Horie S,Watanabe T,Ave K

更新日期：1983-04-01 00:00:00

abstract：:Cyclic AMP receptor protein (CRP) plays a key role in the transcription regulation of many prokaryotic genes. Upon the binding of cyclic AMP, CRP is allosterically activated, binds to target DNA sites, and interacts with RNA polymerase. Although the protein-protein interaction between CRP and RNA polymerase is known t...

journal_title：Journal of biochemistry

authors： Lee TW,Won HS,Park SH,Kyogoku Y,Lee BJ

更新日期：2001-07-01 00:00:00

abstract：:The cytosol of human erythrocytes was found to contain a Ca2+-dependent thiol protease (calpain) and its specific inhibitor (calpastatin) by DEAE-cellulose chromatography at pH 8.0, although no proteolytic activity toward casein was detected in the unfractionated hemolysate. The protease required only 40 microM Ca2+ f...

journal_title：Journal of biochemistry

authors： Murakami T,Hatanaka M,Murachi T

更新日期：1981-12-01 00:00:00

abstract：:A full-length cDNA, which encodes a human placental fructose-6-phosphate,2-kinase/ fructose-2,6-bisphosphatase, was constructed and expressed in Escherichia coli. The expressed protein, purified to homogeneity, showed a molecular weight of 58,000 by gel electrophoresis under denaturing conditions, compared to the dedu...

journal_title：Journal of biochemistry

authors： Sakakibara R,Kato M,Okamura N,Nakagawa T,Komada Y,Tominaga N,Shimojo M,Fukasawa M

更新日期：1997-07-01 00:00:00

abstract：:ENO1-'lacZ fusions with various lengths of the ENO1 5'-flanking region were constructed on various types of yeast plasmid vectors. The fully expressed level of beta Gal directed by ENO1-'lacZ fusions differed depending on the type of vector, but on any type of vector, beta Gal activity was not greatly influenced by th...

journal_title：Journal of biochemistry

authors： Uemura H,Shiba T,Machida M,Matsui I,Jigami Y,Tanaka H

更新日期：1987-07-01 00:00:00

abstract：:beta-Glycosidase from the extreme thermophilic archaeon Sulfolobus solfataricus is a tetrameric protein with a molecular mass of 240 kDa, stable in the presence of detergents, and with a maximal activity at temperatures above 95 degrees C. Understanding the structure-activity relationships of the enzyme under differen...

journal_title：Journal of biochemistry

authors： D'Auria S,Nucci R,Rossi M,Bertoli E,Tanfani F,Gryczynski I,Malak H,Lakowicz JR

更新日期：1999-09-01 00:00:00

abstract：:The enzymatic properties of novel cysteine proteases D3-alpha and beta which were purified from germinating soybean cotyledons were investigated. The enzyme activities were exhibited in the presence of a thiol reagent, such as 2-mercaptoethanol, and apparently inhibited by E-64, a cysteine protease inhibitor. Hydrolyt...

journal_title：Journal of biochemistry

authors： Asano M,Suzuki S,Kawai M,Miwa T,Shibai H

更新日期：1999-08-01 00:00:00

abstract：:The reaction of D-amino acid oxidase [EC 1.4.3.3] (DAO) from porcine kidney with beta-cyano-D-alanine (D-BCNA) was studied. DAO was found to catalyze elimination of the cyano group as well as oxidation of D-BCNA. During the course of the reaction in the presence of excess oxygen, an intermediate was observed which exh...

journal_title：Journal of biochemistry

authors： Miura R,Shiga K,Miyake Y,Watari H,Yamano T

更新日期：1980-05-01 00:00:00

abstract：:Interaction between protein and phospholipid molecules in sarcoplasmic reticulum (SR) was studied by measurement of the conformational fluctuation of the protein using the kinetics of the hydrogen-deuterium exchange reaction. It was revealed that the state of SR ATPase undergoes a phase transition at about 18 degrees ...

journal_title：Journal of biochemistry

authors： Kirino Y,Anzai K,Shimizu H,Ohta S,Nakanishi M,Tsuboi M

更新日期：1977-10-01 00:00:00

abstract：:We studied the effect of Ca2+ on the reactivities of two SH groups of DTNB light chain (Cys 128 and Cys 157) using a fluorogenic thiol reagent. It was found that a Ca2+-induced change in reactivity occurred only with Cys 128 when the light chain was in an isolated state, whereas it occurred with both Cys 128 and Cys 1...

journal_title：Journal of biochemistry

authors： Yamamoto K,Honjo R,Sekine T

更新日期：1980-01-01 00:00:00

abstract：:The Escherichia coli phenylalanine-sensitive DAHP (3-deoxy-D-arabino-heptulosonate 7-phosphate) synthetase (aroG product) is one of the DAHP synthetase isozymes that catalyze the first committed step in the biosynthesis of aromatic amino acids and vitamins. Through target-directed mutagenesis of the cloned aroG on a p...

journal_title：Journal of biochemistry

authors： Ger YM,Chen SL,Chiang HJ,Shiuan D

更新日期：1994-11-01 00:00:00

abstract：:Treatment of Sendai virus with p-azidophenyl-6-chloro-6-deoxy-beta-D-glucopyranoside (APG) caused chemical modification of the viral envelope under UV irradiation, which did not affect the hemagglutinin activity of the virus but inhibited the hemolytic activity. Also, the transfer of phospholipid from the viral envelo...

journal_title：Journal of biochemistry

authors： Jizomoto H,Arita H,Sugita K,Kawanami J,Sato K,Kuriyama K

更新日期：1980-10-01 00:00:00

abstract：:Rat brain type II (beta) protein kinase C (PKC) was phosphorylated by rat lung casein kinase II (CK-II). Neither type I (gamma) nor type III (alpha) PKC was significantly phosphorylated by CK-II. CK-II incorporated 0.2-0.3 mol of phosphate into 1 mol of type II PKC. This phosphate was located at the single seryl resid...

journal_title：Journal of biochemistry

authors： Tominaga M,Kitagawa Y,Tanaka S,Kishimoto A

更新日期：1991-10-01 00:00:00