5-Aminoimidazole-4-carboxyamide-1-β-D-ribofranoside stimulates the rat enhancer of split- and hairy-related protein-2 gene via atypical protein kinase C lambda.

abstract：:Two cDNA clones, pHPah1 and pHPah2, encoding polycyclic hydrocarbon-inducible forms of rabbit liver microsomal cytochrome P-450 were isolated and their nucleotide sequences were determined. The inserts of pHPah1 and pHPah2 contained open reading frames specifying the entire primary structures of cytochrome P-450s, con...

journal_title：Journal of biochemistry

authors： Kagawa N,Mihara K,Sato R

更新日期：1987-06-01 00:00:00

abstract：:Mitochondria play many critical roles in cells, not only by supplying energy, but also by supplying metabolites, buffering Ca2+ levels and regulating apoptosis. During oocyte maturation and subsequent embryo development, mitochondria change their morphology by membrane fusion and fission, and coordinately undergo mult...

journal_title：Journal of biochemistry

authors： Udagawa O,Ishihara N

更新日期：2020-03-01 00:00:00

abstract：:The direct ion-dipolar interactions between potassium ion (K(+)) and the two hydroxyl groups of the substrate are the most striking feature of the crystal structure of coenzyme B(12)-dependent diol dehydratase. We carried out density-functional-theory computations to determine whether K(+) can assist the 1,2-shift of ...

journal_title：Journal of biochemistry

authors： Toraya T,Yoshizawa K,Eda M,Yamabe T

更新日期：1999-10-01 00:00:00

abstract：:At the initiation of chromosomal DNA replication, DNA primases synthesize short RNA primers, which are subsequently elongated by DNA polymerases. To understand the structural basis for the primer synthesis by archaeal/eukaryotic-type primases, the gene of the DNA primase from hyperthermophilic archaeon Pyrococcus hori...

journal_title：Journal of biochemistry

authors： Ito N,Nureki O,Shirouzu M,Yokoyama S,Hanaoka F

更新日期：2001-12-01 00:00:00

abstract：:The in vivo effects of convulsant drugs (hydrazine and penicillamine) on the metabolism of gamma-aminobutyric acid (GABA) in subcellular fractions of mouse brain were studied. Both substances inhibited the activity of glutamic acid decarboxylase [EC 4.1.1.15] (GAD) in the synaptosomal fraction (nerve ending particles)...

journal_title：Journal of biochemistry

authors： Abe M,Matsuda M

更新日期：1977-07-01 00:00:00

abstract：:The stimulatory effects of glucose and lactate on protein synthesis by round spermatids (steps 1-8) were further studied. When the cells were incubated with lactate, the response of protein synthesis in round spermatids was closely related to the intracellular level of ATP. The ATP level in spermatids increased to 3.1...

journal_title：Journal of biochemistry

authors： Nakamura M,Hino A,Kato J

更新日期：1981-10-01 00:00:00

abstract：:1. New methods of preparing troponins from slow skeletal and cardiac muscle of the chicken have been developed. The electrophoretic mobilities of slow skeletal muscle troponin subunits were different from those of the corresponding fast skeletal muscle subunits. 2. A new method for determining the amount of divalent c...

journal_title：Journal of biochemistry

authors： Kohama K

更新日期：1979-09-01 00:00:00

abstract：:Triacylglycerol lipase activities in adrenals and testes of rats were measured and the properties of the enzymes were characterized. The enzymes were extracted from acetone-ether dried powder of the tissues and the crude extracts were applied to a heparin-Sepharose affinity column. The enzymes bound to the column were...

journal_title：Journal of biochemistry

authors： Murase T,Yamada N,Akanuma Y,Ohsawa N

更新日期：1982-09-01 00:00:00

abstract：:cAMP and Ca2(+)-independent histone kinase was generated from rat liver plasma membrane in an ionic strength-dependent manner by the action of an endogenous trypsin-like protease (Hashimoto, E. et al. (1986) FEBS Lett. 200, 63-66). In addition to the effect of ionic strength, this proteolytic activation of protein kin...

journal_title：Journal of biochemistry

authors： Hashimoto E,Yamamura H

更新日期：1989-12-01 00:00:00

abstract：:Chemical modification of gamma-carboxyglutamic acid (Gla) residues in human prothrombin to gamma-methyleneglutamic acid (gamma-MGlu) residues elicited a conformation similar, if not identical, to that of des-gamma-carboxy prothrombin or PIVKA-II, i.e., prothrombin molecules induced by vitamin K antagonists or vitamin ...

journal_title：Journal of biochemistry

authors： Sugo T,Watanabe K,Naraki T,Matsuda M

更新日期：1990-09-01 00:00:00

abstract：:We previously reported that caldesmon (CaD), together with tropomyosin (TM), effectively protects actin filaments from gelsolin, an actin-severing protein. To elucidate the structure/function relationship of CaD, we dissected the functional domain of CaD required for the protection. The basic C-terminal half of rat no...

journal_title：Journal of biochemistry

authors： Takiguchi K,Matsumura F

更新日期：2005-12-01 00:00:00

abstract：:Certain proteins utilize the high reactivity of radicals for catalysing chemically challenging reactions. These proteins contain or form a radical and therefore named 'radical enzymes'. Radicals are introduced by enzymes themselves or by (re)activating proteins called (re)activases. The X-ray structures of radical enz...

journal_title：Journal of biochemistry

authors： Shibata N,Toraya T

更新日期：2015-10-01 00:00:00

abstract：:Serum-free culture medium collected from primary monolayer cultures of human articular chondrocytes was found to inhibit human urokinase [EC 3.4.21.31] activity. Although chondrocyte culture medium contained a small amount of endothelial-type plasminogen activator inhibitor which could be demonstrated by reverse fibri...

journal_title：Journal of biochemistry

authors： Yamada H,Stephens RW,Nakagawa T,McNicol D

更新日期：1988-12-01 00:00:00

abstract：:The transcription and replication of influenza RNA can be studied in vitro by the reconstitution of functional ribonucleoprotein (RNP) complex from viral core proteins including the RNA polymerase (complex of three P protein subunits) and nucleoprotein (NP), and model templates. Here, two different core protein prepar...

journal_title：Journal of biochemistry

authors： Seong BL,Kobayashi M,Nagata K,Brownlee GG,Ishihama A

更新日期：1992-04-01 00:00:00

abstract：:Fluorescence titration curves of 2-[4'-iodoacetamido)anilino)naphthalene-6-sulfonic acid-labeled troponin (IAANS-labeled Tn) and troponin-1-anilinonaphthalene-8-sulfonic acid (Tn-ANS) complex indicated that the fluorescent moiety, IAANS or ANS, detects conformational change of troponin I (TnI) or Tn due to the Ca2+ bi...

journal_title：Journal of biochemistry

authors： Nishio T,Iio T

更新日期：1983-09-01 00:00:00

abstract：:We prepared a streptavidin-based neoglycoprotein which carries more than 140 GT1b oligosaccharides. GT1b oligosaccharides were covalently coupled to streptavidin by reductive amination, yielding a monomer form of streptavidin carrying 13 oligosaccharides. The monomer form of glycosylated streptavidin was polymerized w...

journal_title：Journal of biochemistry

authors： Hashimoto Y,Suzuki M,Crocker PR,Suzuki A

更新日期：1998-03-01 00:00:00

abstract：:Porcine adrenocortical lysosomes were characterized by differential centrifugation, acid hydrolase contents, latency of cathepsin D, release of bound acid hydrolases in soluble form, and isopycnic density gradient centrifugation. Cathepsins D and B, beta-N-acetylglucosaminidase, beta-galactosidase and arylsulphatase w...

journal_title：Journal of biochemistry

authors： Taguchi S,Watabe S,Kouyama H,Tatsunami S,Yago N

更新日期：1981-05-01 00:00:00

abstract：:Binding of native connectin (2,100 kDa fragment of alpha-connectin) to myosin filaments was investigated using a sedimentation technique and densitometric estimations of the separated proteins. In the presence of 60 mM KCl and 5 mM phosphate buffer, pH 7.0, as much as 1.5 mol of connectin was bound to 1 mol of myosin,...

journal_title：Journal of biochemistry

authors： Murayama T,Nakauchi Y,Kimura S,Maruyama K

更新日期：1989-02-01 00:00:00

abstract：:The three-dimensional structure of the purple intermediate of porcine kidney D-amino acid oxidase (DAO) was solved by cryo-X-ray crystallography; the purple intermediate is known to comprise a complex between the dehydrogenated product, an imino acid, and the reduced form of DAO. The crystalline purple intermediate wa...

journal_title：Journal of biochemistry

authors： Mizutani H,Miyahara I,Hirotsu K,Nishina Y,Shiga K,Setoyama C,Miura R

更新日期：2000-07-01 00:00:00

abstract：:OS-9 gene is frequently coamplified with CDK4 gene in human sarcomas. We isolated and characterized three isoforms of OS-9 cDNA found in a myeloid leukemia HL-60 cDNA library. Isoform 1 consisted of 2,700 bp, from which a 667 amino acid sequence was deduced and found to be identical with that of OS-9 cDNA from osteosa...

journal_title：Journal of biochemistry

authors： Kimura Y,Nakazawa M,Yamada M

更新日期：1998-05-01 00:00:00

abstract：:As a new strategy for drug discovery and development, I focus on drug re-profiling as a way to identify new treatments for diseases. In this strategy, the actions of existing medicines, whose safety and pharmacokinetic effects in humans have already been confirmed clinically and approved for use, are examined comprehe...

journal_title：Journal of biochemistry

authors： Mizushima T

更新日期：2011-05-01 00:00:00

abstract：:The internal ribosome entry site (IRES) is important for translation of hepatitis C virus (HCV) mRNA and has a unique RNA structure containing conserved domains I to IV. To investigate the function of domain II, we selected RNA aptamers that bind to domain II of HCV IRES by applying a simple and convenient selection m...

journal_title：Journal of biochemistry

authors： Kikuchi K,Umehara T,Fukuda K,Hwang J,Kuno A,Hasegawa T,Nishikawa S

更新日期：2003-03-01 00:00:00

abstract：:A large number of prokaryotic as well as eukaryotic proteins are produced with amino terminal propeptides. These amino-terminal extensions are essential for mediating proper folding of their corresponding proteins and are also termed as intramolecular chaperones. Though these propeptides are highly specific and unique...

journal_title：Journal of biochemistry

authors： Shinde U,Inouye M

更新日期：1994-04-01 00:00:00

abstract：:D-type cyclin-dependent kinases (Cdk4 and Cdk6) regulate the G1 to S phase progression of the mammalian cell cycle. It has been suggested that Cdk4 and Cdk6 may have distinct functions in vivo, even though they are indistinguishable biochemically. Here we show that although these Cdks phosphorylate multiple residues i...

journal_title：Journal of biochemistry

authors： Takaki T,Fukasawa K,Suzuki-Takahashi I,Semba K,Kitagawa M,Taya Y,Hirai H

更新日期：2005-03-01 00:00:00

abstract：:The physicochemical nature of an active fragment (T2A) of colicin E3 was examined together with those of its specific inhibitor, B, and the T2A-B complex under various conditions by means of CD and fluorescence spectroscopy. 1. T2A was mainly composed of unordered structure, although the molecule was folded into a rat...

journal_title：Journal of biochemistry

authors： Suzuki K,Ohno S,Imahori K

更新日期：1980-03-01 00:00:00

abstract：:A novel positively charged planar bilayer membrane was formed from a mixture containing 20% cationic lipid, 1,2-dioleoyloxy-3-(trimethylammonio)propane, and neutral phospholipid mixture of 56% phosphatidylethanolamine and 24% egg phosphatidylcholine. The basic properties of the bilayer were essentially the same as tho...

journal_title：Journal of biochemistry

authors： Anzai K,Masumi M,Kawasaki K,Kirino Y

更新日期：1993-10-01 00:00:00

abstract：:The asparagine-linked sugar chains of human follicle-stimulating hormone (hFSH) were liberated as radioactive oligosaccharides from the polypeptide moiety by hydrazinolysis followed by N-acetylation and NaB3H4 reduction. Ninety-five percent of the oligosaccharides were acidic and all were converted to a mixture of neu...

journal_title：Journal of biochemistry

authors： Renwick AG,Mizuochi T,Kochibe N,Kobata A

更新日期：1987-05-01 00:00:00

abstract：:An acid proteinase different from pepsin was purified from bullfrog (Rana catesbeiana) gastric mucosa by chromatography on hydroxyapatite, Q-Sepharose, Con A-Sepharose 4B, and Mono Q columns. Its molecular weight after purification was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 45 kDa...

journal_title：Journal of biochemistry

authors： Inokuchi T,Kobayashi K,Horiuchi S

更新日期：1994-01-01 00:00:00

abstract：:Photoaffinity labeling with [3H]cytochalasin B detects two D-glucose-sensitive proteins in the chicken embryo fibroblast (CEF) plasma membrane, which accumulate under conditions of glucose starvation and are probably involved in the glucose transport system (Pessin, J.E., et al. (1982) Proc. Natl. Acad. Sci. U.S. 79, ...

journal_title：Journal of biochemistry

authors： Ishii T,Tillotson LG,Isselbacher KJ

更新日期：1986-01-01 00:00:00

abstract：:The small, monomeric Ca2+-binding photoprotein, aequorin, emits blue light by an intramolecular reaction when mixed with Ca2+. The photoprotein is made up of coelenterazine and molecular oxygen, bound noncovalently to apoaequorin (apoprotein). The chemical steps leading to light emission, involving the oxidative degra...

journal_title：Journal of biochemistry

authors： Inouye S,Aoyama S,Miyata T,Tsuji FI,Sakaki Y

更新日期：1989-03-01 00:00:00