Role of the basic C-terminal half of caldesmon in its regulation of F-actin: comparison between caldesmon and calponin.

abstract：:The X-ray diffraction pattern from chromatophore membranes of a photosynthetic bacterium, Rhodospirillum rubrum, indicates that a highly organized protein assembly exists in the membrane. The X-ray scatterer was solubilized from chromatophores by a mixture of cholate and deoxycholate. The basic component was identifie...

journal_title：Journal of biochemistry

authors： Kataoka M,Ueki T

更新日期：1981-01-01 00:00:00

abstract：:Ovomucoid-trypsin association complex was prepared by incubating chicken egg white ovomucoid with bovine trypsin. The reactivity of ovomucoid-trypsin complex was investigated by immunodiffusion, quantitative precipitation and enzyme-linked immunosorbent assay (ELISA). It was demonstrated that the association of trypsi...

journal_title：Journal of biochemistry

authors： Matsuda T,Watanabe K,Nakamura R

更新日期：1983-07-01 00:00:00

abstract：:Human colostral macrophages stimulated by opsonized zymosan or phorbol myristate acetate (PMA) released superoxide anions (O2-) and hydrogen peroxide (H2O2) with activities comparable to those of monocytes and about one-fourth of those of polymorphonuclear leukocytes (PMNL) of blood. The O2- -forming oxidase in the ma...

journal_title：Journal of biochemistry

authors： Tsuda H,Takeshige K,Shibata Y,Minakami S

更新日期：1984-05-01 00:00:00

abstract：:Corynebacterium diphtheriae, a pathogenic Gram-positive bacterium, contains sialic acids on its cell surface, but no genes related to sialic acid decoration or metabolism have been reported in C. diphtheriae. In the present study, we have identified a putative sialidase gene, nanH, from C. diphtheriae KCTC3075 and cha...

journal_title：Journal of biochemistry

authors： Kim S,Oh DB,Kwon O,Kang HA

更新日期：2010-04-01 00:00:00

abstract：:In the crude extract of matured eggs of the silkworm (Bombyx mori), proteolytic activity was detected only in the acidic pH region with maximal activity at pH 3.5, and this activity was maintained throughout the development. No appreciable activity was observed in the neutral to alkaline pH region either in matured eg...

journal_title：Journal of biochemistry

authors： Kageyama T,Takahashi SY,Takahashi K

更新日期：1981-09-01 00:00:00

abstract：:Expression of structural proteins of human immunodeficiency virus type 1 requires the direct interaction of the viral Rev trans-activator with its cis-acting RNA target sequence, the Rev response element (RRE). The originally defined 234-residue RRE, however, failed to show the full HIV-1 regulator of virion expressio...

journal_title：Journal of biochemistry

authors： Kimura T,Ohyama A

更新日期：1994-05-01 00:00:00

abstract：:The accumulation of unfolded proteins in the endoplasmic reticulum (ER) under ER stress conditions activates a series of homoeostatic responses collectively termed the unfolded protein response (UPR). The UPR is unique in which the molecular mechanisms it uses to transmit signals from the ER lumen to the nucleus are c...

journal_title：Journal of biochemistry

authors： Mori K

更新日期：2009-12-01 00:00:00

abstract：:Rat liver glucocorticoid receptor was partially purified and characterized for its hormone binding using selenite. Selenite at very low concentrations irreversibly inhibited the hormone binding. The concentration for half maximal inhibition was approximately 2.8 microM. The inhibition was restored by dithiothreitol. T...

journal_title：Journal of biochemistry

authors： Tashima Y,Terui M,Itoh H,Mizunuma H,Kobayashi R,Marumo F

更新日期：1989-03-01 00:00:00

abstract：:The amino acid sequences of ferredoxins I and II from a blue-green alga, Nostoc strain MAC were determined. This alga is able to grow autotrophically in the light or heterotrophically in the dark. Analyses of tryptic peptides of Cm-proteins by conventional methods including solid-phase Edman degradation gave the compl...

journal_title：Journal of biochemistry

authors： Hase T,Matsubara H,Hutber GN,Rogers LJ

更新日期：1982-11-01 00:00:00

abstract：:Papain [EC 3.4.22.2] polymerizes readily upon treatment with tetranitromethane (TNM) by forming intermolecular covalent linkages through its tyrosine residues (Tsukamoto, S. & Ohno, M. (1974) J. Biochem. 75, 1377-1380). Polymerization occurred optimally at pH 9.0 with S-sulfenylsulfonate papain. Circular dichroic spec...

journal_title：Journal of biochemistry

authors： Tsukamoto S,Ohno M

更新日期：1978-12-01 00:00:00

abstract：:Previously, we reported that proteasomes (large multi-protease complexes) are present in a latent state in a variety of eukaryotic cells, and can be activated by treatment with various compounds such as sodium dodecyl sulfate (SDS) or poly-lysine (Tanaka et al. (1988) J. Biol. Chem. 263, 16209-16217). In the present s...

journal_title：Journal of biochemistry

authors： Tanaka K,Yoshimura T,Ichihara A

更新日期：1989-09-01 00:00:00

abstract：:We isolated three genomic clones of rat P-450(11 beta) genes (CYP11B). Two of them corresponded to 11 beta-hydroxylase gene (CYP11B1) and aldosterone synthase gene (CYP11B2), respectively. The third one was a novel gene resembling both CYP11B1 and CYP11B2, and was named CYP11B3 gene (CYP11B3). CYP11B2 and CYP11B3 are ...

journal_title：Journal of biochemistry

authors： Nomura M,Morohashi K,Kirita S,Nonaka Y,Okamoto M,Nawata H,Omura T

更新日期：1993-02-01 00:00:00

abstract：:The amount of S-II, a protein specifically stimulating RNA polymerase II, was measured in various mouse organs by a micro complement fixation assay. The amount was almost the same in brain, liver, kidney, spleen, and Ehrlich ascites tumor cells on the basis of DNA, being about 3.8 micrograms/mg DNA, which corresponds ...

journal_title：Journal of biochemistry

authors： Tamura H,Sekimizu K,Obinata M,Natori S

更新日期：1980-11-01 00:00:00

abstract：:The kinetic specificities of BPN' and Carlsberg subtilisins [EC 3.4.21.14] were examined with various nucleus-substituted derivatives of Nalpha-acetylated aromatic amino acid methyl esters for mapping their hydrophobic binding sites in comparison with that of alpha-chymotrypsin. The Carlsberg enzyme was generally much...

journal_title：Journal of biochemistry

authors： Karasaki Y,Ohno M

更新日期：1978-09-01 00:00:00

abstract：:The sugar chain of bovine pancreatic ribonuclease B was released from the polypeptide moiety by hydrazinolysis and by endo-beta-N-acetylglucosaminidase H digestion, and reduced with NaB[3H]4 after N-acetylation. The radioactive oligosaccharide mixtures thus obtained were fractionated by Bio-Gel P-4 column chromatograp...

journal_title：Journal of biochemistry

authors： Liang CJ,Yamashita K,Kobata A

更新日期：1980-07-01 00:00:00

abstract：:We describe cross-linking conditions for myosin subfragment-1 and actin with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide, with which the compositions of cross-linked species can be altered. The specific Mg-ATPase activity of cross-linked subfragment-1 in these preparations was almost the same in spite of the diffe...

journal_title：Journal of biochemistry

authors： Yamamoto K

更新日期：1993-12-01 00:00:00

abstract：:Heptic fibrosis/cirrhosis is a common hepatic disease characterized by the hyper-accumulation of connective tissue components, and hepatic necrosis. Chronic alcohol ingestion, viral infection, and metabolic disorders are contributing factors and there has been no effective treatment. Hepatocyte growth factor (HGF), or...

journal_title：Journal of biochemistry

authors： Matsuda Y,Matsumoto K,Ichida T,Nakamura T

更新日期：1995-09-01 00:00:00

abstract：:The complete amino acid sequence of acylphosphatase from rabbit skeletal muscle has been elucidated by automatic Edman degradation of peptides obtained from staphylococcal protease and trypsin digestions. The enzyme consisted of a single polypeptide chain of 98 amino acid residues, lacking only histidine. Its amino (N...

journal_title：Journal of biochemistry

authors： Kizaki T,Takasawa T,Mizuno Y,Shiokawa H

更新日期：1985-04-01 00:00:00

abstract：:beta-D-Galactosidase has been purified to apparent homogeneity from rabbit spleen. The purification steps involved ammonium sulphate precipitation, DEAE-cellulose, concanavalin A-Sepharose, Sephadex G-200, and Sepharose 4B-(epsilon-aminocaproyl)-2-deoxy-beta-D-glucosylamine affinity chromatographies. In the DEAE-cellu...

journal_title：Journal of biochemistry

authors： Rodríguez-Berrocal FJ,Páez de la Cadena M,Cabezas JA,Pérez-González N

更新日期：1988-07-01 00:00:00

abstract：:Fluorescence changes of 1-anilino-8-naphthalenesulfonic acid and 3,3'-dipropyl-2,2'-thiadicarbocyanine during Ca transport by sarcoplasmic reticulum vesicles were studied. The fluorescence of both probes is enhanced corresponding to the rapid initial Ca uptake. The enhancement could be interpreted in terms of increase...

journal_title：Journal of biochemistry

authors： Ueno T,Sekine T

更新日期：1978-10-01 00:00:00

abstract：:Protein S, a vitamin K-dependent plasma protein having Gla-residues, increases the rate of inactivation of Factor Va by activated protein C by enhancing the binding of activated protein C to phospholipid [Walker, J.F. (1981) J. Biol. Chem. 256, 11128-11131]. The present study aimed at elucidating the effect of thrombi...

journal_title：Journal of biochemistry

authors： Suzuki K,Nishioka J,Hashimoto S

更新日期：1983-09-01 00:00:00

abstract：:Phospholipase A2 (PLA2) has been purified to homogeneity from human arthritic synovial fluid. The activity resolved into multiple peaks by preparative HPLC. The most abundant peak (A) was present in synovial fluid from patients with rheumatoid arthritis, osteoarthritis, and psoriatic arthritis. A second major peak (B)...

journal_title：Journal of biochemistry

authors： Seilhamer JJ,Plant S,Pruzanski W,Schilling J,Stefanski E,Vadas P,Johnson LK

更新日期：1989-07-01 00:00:00

abstract：:The vitamin A derivative, retinoic acid (RA) has various biological effects in mammalian cells and tissues. It is well known that RA induces differentiation of leukemia cells and inhibits cell growth. There are two pathways for RA action; one via RA nuclear receptors (RARs), and one via acylation of proteins by RA (re...

journal_title：Journal of biochemistry

authors： Kubo Y,Ohba T,Takahashi N

更新日期：2008-09-01 00:00:00

abstract：:Porcine adrenocortical lysosomes were characterized by differential centrifugation, acid hydrolase contents, latency of cathepsin D, release of bound acid hydrolases in soluble form, and isopycnic density gradient centrifugation. Cathepsins D and B, beta-N-acetylglucosaminidase, beta-galactosidase and arylsulphatase w...

journal_title：Journal of biochemistry

authors： Taguchi S,Watabe S,Kouyama H,Tatsunami S,Yago N

更新日期：1981-05-01 00:00:00

abstract：:Triacylglycerol lipase activities in adrenals and testes of rats were measured and the properties of the enzymes were characterized. The enzymes were extracted from acetone-ether dried powder of the tissues and the crude extracts were applied to a heparin-Sepharose affinity column. The enzymes bound to the column were...

journal_title：Journal of biochemistry

authors： Murase T,Yamada N,Akanuma Y,Ohsawa N

更新日期：1982-09-01 00:00:00

abstract：:Chain elongation of fusaric acid and related compounds in the presence of rat liver preparations was investigated by gas chromatography-mass spectrometry. The mitochondrial fraction catalyzed the elongation of the CoA esters of fusaric acid and 5-butyl-2-pyrimidinecarboxylic acid utilizing acetyl-CoA as a C2 donor. Th...

journal_title：Journal of biochemistry

authors： Mizugaki M,Sagi M,Yamanaka H,Takayama H,Ishibashi M,Miyazaki H

更新日期：1986-02-01 00:00:00

abstract：:Bindings of cobra venom phospholipases A2 to micelles of n-hexadecylphosphorylcholine were studied by the tryptophyl fluorescence method at 25 degrees C and ionic strength 0.1. The data were analyzed by assuming that the micellar surface has multiple binding sites for the enzyme and these sites are identical and mutua...

journal_title：Journal of biochemistry

authors： Teshima K,Ikeda K,Hamaguchi K,Hayashi K

更新日期：1983-07-01 00:00:00

abstract：:Chalcone synthase was purified to homogeneity by polyacrylamide gel electrophoresis from cell suspension cultures of carrot in which anthocyanin synthesis was induced by transferring the cells from a medium containing 2,4-dichlorophenoxy-acetic acid (2,4-D) to one lacking it. A molecular weight of 80,000-85,000 for th...

journal_title：Journal of biochemistry

authors： Ozeki Y,Sakano K,Komamine A,Tanaka Y,Noguchi H,Sankawa U,Suzuki T

更新日期：1985-07-01 00:00:00

abstract：:A cyclodextrin glucanotransferase (CGTase) from Bacillus clarkii 7364 converts starch into gamma-cyclodextrin (gamma-CD) with high specificity. Comparison of the deduced amino acid sequence of this CGTase with those of other typical CGTases revealed that several amino acids are deleted or substituted with others at se...

journal_title：Journal of biochemistry

authors： Nakagawa Y,Takada M,Ogawa K,Hatada Y,Horikoshi K

更新日期：2006-09-01 00:00:00

abstract：:Dupuytren's disease is a palmar fibromatosis associated with changes in fibroblast activity that also affect the metabolism of extracellular matrix components. In contrast to disease connected alterations in collagen and non-collagenous glycoproteins (mainly fibronectin), the metabolism of proteoglycans, being glycosa...

journal_title：Journal of biochemistry

authors： Koźma EM,Olczyk K,Wisowski G,Głowacki A,Bobiński R

更新日期：2005-04-01 00:00:00