Regulation of activated protein C by thrombin-modified protein S.

abstract：:The formation of cholesta-7,24-dien-3 beta-ol and its activity as a substrate for the sterol side-chain methyltransferase in yeast have not previously been studied. Experiments with acetone-powder extracts of yeast showed that the sterol is formed from zymosterol by delta8-delta7 isomerization. However, direct convers...

journal_title：Journal of biochemistry

authors： Yabusaki Y,Nishino T,Ariga N,Katsuki H

更新日期：1979-06-01 00:00:00

abstract：:In order to obtain information on the nature of the amino acid residues involved in the activity of ribonuclease U1 [EC 3.1.4.8], various chemical modifications of the enzyme were carried out. RNase U1 was inactivated by reaction with iodoacetate at pH 5.5 with concomitant incorporation of 1 carboxymethyl group per mo...

journal_title：Journal of biochemistry

authors： Hashimoto J,Takahashi K

更新日期：1977-04-01 00:00:00

abstract：:Mitochondrial processing peptidase (MPP), a metalloendopeptidase consisting of alpha and beta subunits, specifically recognizes a large variety of mitochondrial precursor proteins and cleaves off the N-terminal basic matrix targeting signals (MTS). Basic residues in MTS and acidic sites in MPP are required for effecti...

journal_title：Journal of biochemistry

authors： Kitada S,Ito A

更新日期：2001-01-01 00:00:00

abstract：:A heat-labile, non-dialyzable factor(s) in soluble fractions from Escherichia coli strains and Bacillus subtilis was found to incorporate the radioactivity of [14C]glutamic acid into 95 degrees C CCl3COOH-insoluble fraction. Incorporation catalyzed by a partially purified factor from E. coli B required ATP, Mg2+, tRNA...

journal_title：Journal of biochemistry

authors： Hashizume S,Honjoh T,Shoji M

更新日期：1983-04-01 00:00:00

abstract：:The presence of a leucine zipper motif was recognized in the deduced amino acid sequences of human and rat renin-binding proteins (RnBPs) on cloning and sequence analysis of the RnBP cDNAs. The in vitro synthesized RnBPs, with the respective cDNAs, formed heterodimers with porcine renin and homodimers. On comparison o...

journal_title：Journal of biochemistry

authors： Inoue H,Takahashi S,Fukui K,Miyake Y

更新日期：1991-10-01 00:00:00

abstract：:Long non-coding RNA is an endogenous non-coding RNA that has currently been proved to be an important player in cancer cell biology. In the present study, we investigated the biological role of PHACTR2-AS1 in tongue squamous cell carcinoma (TSCC). PHACTR2-AS1 was preferentially localized in the cytoplasm, and was nota...

journal_title：Journal of biochemistry

authors： Yuan F,Miao Z,Chen W,Wu F,Wei C,Yong J,Xiao C

更新日期：2020-12-26 00:00:00

abstract：:To investigate a possible function of plasma albumin in partitioning organic anions into bile and urine, phenolsulfonphthalein (PSP) was administered intravenously and its in vivo fate was studied in normal and analbuminemic mutant rats (NAR). No significant change in the rate of PSP disappearance was observed in bila...

journal_title：Journal of biochemistry

authors： Inoue M,Koyama H,Nagase S,Morino Y

更新日期：1986-07-01 00:00:00

abstract：:Mild trypsinization of human erythrocyte stroma solubilized CR1 (complement receptor type 1, C3b/C4b receptor) without significant loss of decay-accelerating activity to C5 convertases on hemolytic intermediate cells (EAC 1-3b, P). The solubilized CR1 was purified using DEAE-Sephacel, C3-Sepharose, and anti-CR1-Sephar...

journal_title：Journal of biochemistry

authors： Sugita Y,Nakano Y,Tomita M

更新日期：1986-11-01 00:00:00

abstract：:A highly sensitive method was developed for quantitative analysis of phospholipid molecular species. Diradylglycerols prepared from phospholipids with phospholipase C were converted to the anthroyl-diradylglycerol derivatives, which could be separated into molecular species and sensitively quantified by reverse-phase ...

journal_title：Journal of biochemistry

authors： Takamura H,Kito M

更新日期：1991-03-01 00:00:00

abstract：:We recently described the cloning of putative human CMP-sialic acid transporter (hCST) cDNA [Ishida, N. et al. (1996) J. Biochem. 120, 1074-1078]. The hCST cDNA coded for a hydrophobic protein with an amino acid sequence showing a high degree of similarity (92% identity) to that of murine CMP-sialic acid transporter. ...

journal_title：Journal of biochemistry

authors： Ishida N,Ito M,Yoshioka S,Sun-Wada GH,Kawakita M

更新日期：1998-07-01 00:00:00

abstract：:The structure of polygalactosamine purified from the culture fluid of Aspergillus parasiticus AHU 7165 was studied. Partial acid hydrolysis of this polysaccharide, in which 55 to 65% of the monosaccharide residues were N-unsubstituted, gave a series of galactosamine oligosaccharides (dimer to hexamer) in a good yield....

journal_title：Journal of biochemistry

authors： Takada H,Araki Y,Ito E

更新日期：1981-04-01 00:00:00

abstract：:In the crude extract of matured eggs of the silkworm (Bombyx mori), proteolytic activity was detected only in the acidic pH region with maximal activity at pH 3.5, and this activity was maintained throughout the development. No appreciable activity was observed in the neutral to alkaline pH region either in matured eg...

journal_title：Journal of biochemistry

authors： Kageyama T,Takahashi SY,Takahashi K

更新日期：1981-09-01 00:00:00

abstract：:Apoprotein of electron-transferring flavoprotein (ETF) reacts with FAD as follows: A*<-->A, A+FAD<-->holoETF. Two different forms of apoETF (A* and A) convert into each other and only one of them, A, can associate with FAD [Sato, K. et al. (1991) J. Biochem. 109, 734-740]. In the present study, the reactions between a...

journal_title：Journal of biochemistry

authors： Sato K,Nishina Y,Shiga K

更新日期：1992-12-01 00:00:00

abstract：:Sialoglycoprotein which exhibits inhibitory activity for hemagglutination by Hemagglutinating Virus of Japan (HVJ, Sendai virus) was isolated from the membrane of bovine erythrocytes. Purification steps for this sialoglycoprotein included extraction with lithium diiodosalicylate, phenol partition, precipitation with e...

journal_title：Journal of biochemistry

authors： Suzuki Y,Suzuki T,Matsumoto M

更新日期：1983-06-01 00:00:00

abstract：:Genetic engineering strategies were applied to synthesize silk-like materials, [(GVPGV)(2)GG(GAGAGS)(3)AS](n). The primary structure of these materials represents the repetitive crystalline region of Bombyx mori silk fibroins incorporated with an elastic motif selected from animal elastin. The oligonucleotides were de...

journal_title：Journal of biochemistry

authors： Yao J,Asakura T

更新日期：2003-01-01 00:00:00

abstract：:The unfolded protein response is an adaptive stress response that responds to the imbalance between the entry of newly synthesized unfolded proteins and the inherent folding capacity in the endoplasmic reticulum (ER). Various environmental stresses and changes in physiological conditions can result in the accumulation...

journal_title：Journal of biochemistry

authors： Kohno K

更新日期：2010-01-01 00:00:00

abstract：:OsCnfU-1A is a chloroplast-type Nfu-like protein that consists of tandem repeats sharing high sequence homology. Domain I of this protein, but not domain II, has a C-X-X-C motif that is thought to assemble an iron-sulphur cluster. Herein we report the solution structure of OsCnfU-1A domain I (73-153). Although OsCnfU-...

journal_title：Journal of biochemistry

authors： Saio T,Kumeta H,Ogura K,Yokochi M,Asayama M,Katoh S,Katoh E,Teshima K,Inagaki F

更新日期：2007-07-01 00:00:00

abstract：:The cDNA of a novel protein, which contains the association domain of alpha isoform of calmodulin-dependent protein kinase II (CaM-kinase II alpha), was cloned from rat skeletal muscle. This protein, called alpha KAP, consisted of 200 amino acid residues with a molecular weight of 22,583. alpha KAP has a highly hydrop...

journal_title：Journal of biochemistry

authors： Sugai R,Takeuchi M,Okuno S,Fujisawa H

更新日期：1996-10-01 00:00:00

abstract：:The entire mitochondrial genome of Rana catesbeiana was cloned into a plasmid vector pBR322 at the unique BamHI site and the nucleotide sequences of the ND2 gene and of its flanking genes were determined. The ND2 gene was encoded by 1,033 base pairs and, as deduced from the nucleotide sequence, the ND2 product consist...

journal_title：Journal of biochemistry

authors： Fujii H,Shimada T,Goto Y,Okazaki T

更新日期：1988-03-01 00:00:00

abstract：:The mitogen-activated protein kinase (MAPK) signaling pathway, which is conserved from yeast to humans, is activated in response to a variety of extra- and intracellular stimuli, and plays key roles in multiple cellular processes, including proliferation, differentiation, and apoptosis. The MAPK pathway transmits its ...

journal_title：Journal of biochemistry

authors： Yoshioka K

更新日期：2004-06-01 00:00:00

abstract：:Vertebrate skeletal muscle alpha-tropomyosin polymerizes in a head-to-tail manner and binds cooperatively to actin. It has been postulated that the cooperative actin binding is governed by the strength of the head-to-tail interaction. In order to know the relationship between the head-to-tail affinity and actin bindin...

journal_title：Journal of biochemistry

authors： Sano K,Maeda K,Oda T,Maéda Y

更新日期：2000-06-01 00:00:00

abstract：:Ornithine transcarbamylase (OTC) is one of the urea cycle enzymes. While the chicken is a uricotelic animal, it has measurable OTC activity in its kidney. OTC activity is highly variable within and between chicken breeds. Chicken OTC may have some physiological significance because of its significant activity in the k...

journal_title：Journal of biochemistry

authors： Shimogiri T,Kono M,Mannen H,Mizutani M,Tsuji S

更新日期：1998-11-01 00:00:00

abstract：:The rates of synthesis and degradation of arginosuccinate synthetase in rat liver under various dietary conditions were determined. The relative rate of the enzyme synthesis in the livers of rats fed on 70% casein diet was 4.0 times greater than that for rats fed on 5% casein diet. The rate constants of degradation (K...

journal_title：Journal of biochemistry

authors： Tsuda M,Shikata Y,Katsunuma T

更新日期：1979-03-01 00:00:00

abstract：:We have previously reported that calpastatin, an endogenous inhibitory protein of calpain, is cleaved by a caspase-3-like protease during apoptosis in human Jurkat T cells [Kato, M. et al. (2000) J. Biochem. 127, 297-305]. In this study, we found that nonmuscle myosin heavy chain-A (NMHC-A) is cleaved during apoptosis...

journal_title：Journal of biochemistry

authors： Kato M,Fukuda H,Nonaka T,Imajoh-Ohmi S

更新日期：2005-02-01 00:00:00

abstract：:G-actin freed from exogenous ATP was added to the pieces of isolated acrosomal actin bundles from horseshoe crab sperm to form filaments as reported earlier (Tilney, L.G., Bonder, E.M., & DeRosier, D.J. (1981) J. Cell Biol. 90, 485-494). The growth of a filament was far more rapid at one end (the preferred end) than t...

journal_title：Journal of biochemistry

authors： Mabuchi I

更新日期：1983-10-01 00:00:00

abstract：:We previously reported that sera from various kinds of animals contain a protein(s) capable of inhibiting the growth of the non-malignant epithelial cell line derived from Buffalo rat liver (BRL). In the present study, a similar epithelial cell-specific growth inhibitor (EGI) was purified to homogeneity from an acid-e...

journal_title：Journal of biochemistry

authors： Huang W,Kimura T,Mashima K,Miyazaki K,Masaki H,Yamashita J,Horio T

更新日期：1986-09-01 00:00:00

abstract：:Activity-oligomeric assembly relationships using octaethylene glycol dodecyl ether (C12E8) solubilized pig gastric H/K-ATPase (unmodified H/K-ATPase) or H/K-ATPase modified with Fluorescein 5'-isothiocyanate (FITC-H/K-ATPase) were examined. The amount of oligomeric species in FITC-H/K-ATPase, which retained little H/K...

journal_title：Journal of biochemistry

authors： Abe K,Kaya S,Taniguchi K,Hayashi Y,Imagawa T,Kikumoto M,Oiwa K,Sakaguchi K

更新日期：2005-09-01 00:00:00

abstract：:Serum-free culture medium collected from primary monolayer cultures of human articular chondrocytes was found to inhibit human urokinase [EC 3.4.21.31] activity. Although chondrocyte culture medium contained a small amount of endothelial-type plasminogen activator inhibitor which could be demonstrated by reverse fibri...

journal_title：Journal of biochemistry

authors： Yamada H,Stephens RW,Nakagawa T,McNicol D

更新日期：1988-12-01 00:00:00

abstract：:The sugar chain of bovine pancreatic ribonuclease B was released from the polypeptide moiety by hydrazinolysis and by endo-beta-N-acetylglucosaminidase H digestion, and reduced with NaB[3H]4 after N-acetylation. The radioactive oligosaccharide mixtures thus obtained were fractionated by Bio-Gel P-4 column chromatograp...

journal_title：Journal of biochemistry

authors： Liang CJ,Yamashita K,Kobata A

更新日期：1980-07-01 00:00:00

abstract：:When the membrane fraction of Saccharomyces carlsbergensis was incubated with radiolabeled isopentenyl diphosphate in the presence of farnesyl diphosphate and Mg2+, phosphorylated and free long-chain polyprenols were formed. The reaction was inhibited by EDTA and heavy metal cations. A series of non-ionic detergents w...

journal_title：Journal of biochemistry

authors： Bukhtiyarov YE,Shabalin YA,Kulaev IS

更新日期：1993-06-01 00:00:00