ATPase activities of the two cross-linked species of actin and myosin.

abstract：:pKY2289, a ColE1::Tn3 derivative, useful for direct selection of cells carrying a hybrid plasmid, was deleted of its mobility functions and parts of the transposon including the left inverted repeat. This deletion mutant, named pKY2700 expresses low levels of colicin E1 synthesis even in recA cells. A nitrosoguanidine...

journal_title：Journal of biochemistry

authors： Ozaki LS,Kimura A,Shimada K,Takagi Y

更新日期：1982-04-01 00:00:00

abstract：:Of the five HSV1 immediate-early proteins, infected-cell protein 22 (ICP22), the product of the Us1 gene, is a member whose function is less understood. In order to promote better understanding of the role of ICP22 in viral replication, mutation and fluorescence techniques were used to investigate the biochemical rela...

journal_title：Journal of biochemistry

authors： Cun W,Hong M,Liu LD,Dong CH,Luo J,Li QH

更新日期：2006-07-01 00:00:00

abstract：:A 45-60 kDa Gal/GalNAc-specific macrophage lectin was found to participate in the interaction between tumor cells and tumoricidal macrophages activated by an antitumor streptococcal preparation, OK-432, and in the tumoricidal activity of the activated macrophages. The binding between OK-432-elicited activated macropha...

journal_title：Journal of biochemistry

authors： Oda S,Sato M,Toyoshima S,Osawa T

更新日期：1989-06-01 00:00:00

abstract：:Class B scavenger receptor type I (SR-BI) is a phosphatidylserine (PS)-recognizing receptor of testicular Sertoli cells responsible for the phagocytosis of spermatogenic cells undergoing apoptosis. Here, we determined signal mediators that compose a signalling pathway for SR-BI-induced phagocytosis. Results of a yeast...

journal_title：Journal of biochemistry

authors： Osada Y,Sunatani T,Kim IS,Nakanishi Y,Shiratsuchi A

更新日期：2009-03-01 00:00:00

abstract：:We designed mutant lysozymes with N-glycosylation signal sequences (Asn48-Gly49-Thr-50 and Asn87-Ile88-Thr89) by substituting Asp to Asn at positions 48 and 87. When these mutant lysozymes were expressed by using yeast (Saccharomyces cerevisiae) in Burkholder minimum medium, N-glycosylation occurred in both lysozymes....

journal_title：Journal of biochemistry

authors： Ueda T,Iwashita H,Hashimoto Y,Imoto T

更新日期：1996-01-01 00:00:00

abstract：:Spo0A is both a positive and a negative transcriptional regulator which plays a very important role in sporulation initiation in Bacillus subtilis. Its N-terminal amino acid sequence is homologous to that of regulator proteins of the two-component regulatory systems involved in signal transduction in bacteria. Phospho...

journal_title：Journal of biochemistry

authors： Asayama M,Kobayashi Y

更新日期：1993-09-01 00:00:00

abstract：:The binding of ADP to subfragment-1 was investigated by the gel filtration method. The amount of bound ADP was determined as a function of free ADP concentration. Linear Scatchard plots were obtained. The maximum binding number, 0.55 mole of ADP per 10(5) g of protein, and the dissociation constant, 1.6 x 10(-6) M, we...

journal_title：Journal of biochemistry

authors： Yoshida M,Morita F

更新日期：1975-05-01 00:00:00

abstract：:The role of mannan chains in the formation and secretion of active acid phosphatase of yeast (Saccharomyces cerevisiae), a repressible cell surface mannoprotein, was studied in yeast protoplast systems by using tunicamycin at various temperatures. At 30 degrees C, tunicamycin-treated protoplasts did not produce active...

journal_title：Journal of biochemistry

authors： Mizunaga T,Izawa M,Ikeda K,Maruyama Y

更新日期：1988-02-01 00:00:00

abstract：:The flagellar motor is composed of the stator and the rotor, and the interaction between the stator and the rotor at the cytoplasmic region is believed to produce mechanical force for the rotation of flagella. The periplasmic region of the stator has been proposed to play an important role in assembly around and incor...

journal_title：Journal of biochemistry

authors： Hizukuri Y,Kojima S,Homma M

更新日期：2010-09-01 00:00:00

abstract：:Aggregatibacter actinomycetemcomitans is an oral pathogen for aggressive periodontitis, and encodes a triheme c-containing membrane-bound enzyme, quinol peroxidase (QPO) that catalyzes peroxidase activity using quinol in the respiratory chain. In the previous work, we have characterized recombinant QPO purified from t...

journal_title：Journal of biochemistry

authors： Abe T,Kawarai T,Takahashi Y,Konishi K

更新日期：2017-06-01 00:00:00

abstract：:The crystal structure of an adenylate kinase from an extremophilic archaeon Aeropyrum pernix was determined in complex with full ligands, ATP-Mg2+ and AMP, at a resolution of 2.0 Å. The protein forms a trimer as found for other adenylate kinases from archaea. Interestingly, the reacting three atoms, two phosphorus and...

journal_title：Journal of biochemistry

authors： Shibanuma Y,Nemoto N,Yamamoto N,Sampei GI,Kawai G

更新日期：2020-09-01 00:00:00

abstract：:Analysis of the quantitative precipitin reaction of adenylate kinase from porcine skeletal muscle with goat anti-adenylate kinase antiserum indicated that there are at least four antigenic determinants on the enzyme molecule. Porcine adenylate kinase was cleaved with cyanogen bromide, and four peptides were fractionat...

journal_title：Journal of biochemistry

authors： Sato N,Koyama Y,Shiokawa H

更新日期：1982-05-01 00:00:00

abstract：:Aggrecan, a large aggregating proteoglycan, is one of the major structural components of cartilage. Its core protein contains three glubular domains and two glycosaminoglycan-attachment domains. These domains play various roles to maintain cartilage structure and function. An N-terminal globular domain binds hyalurona...

journal_title：Journal of biochemistry

authors： Watanabe H,Yamada Y,Kimata K

更新日期：1998-10-01 00:00:00

abstract：:Four glycolipids (Fr. I, II, III, and IV) were isolated from whale brain and three (Fr. I, II, and III) of them were demonstrated to be galactosyl ceramide combined with fatty acid by ester linkage. Fr. IV was shown to be a monoalkyl monoacyl galactosyl glycerol. For determining the substituted position of the acyl gr...

journal_title：Journal of biochemistry

authors： Yasugi E,Saito E,Kasama T,Kojima H,Yamakawa T

更新日期：1982-04-01 00:00:00

abstract：:Four kinds of active sites of bacterial fatty acid synthetase were mapped on distinct regions within a subunit. Active sites were specifically labeled with radioactive substrates and active-site-directed inhibitors. Labeled enzymes were cleaved with proteases, and the fragments thus produced were identified with respe...

journal_title：Journal of biochemistry

authors： Morishima N,Ikai A

更新日期：1987-12-01 00:00:00

abstract：:We characterized the taurine transport system in human intestinal Caco-2 cells and showed that it is subject to adaptive regulation. The activity of taurine transport in Caco-2 cells was evaluated by means of an Na+- and Cl(-)-dependent high-affinity transport system, the characteristics of which were similar to those...

journal_title：Journal of biochemistry

authors： Satsu H,Watanabe H,Arai S,Shimizu M

更新日期：1997-06-01 00:00:00

abstract：:We have recently developed an in vitro yeast reconstituted translation system, which is capable of synthesizing long polypeptides. Utilizing the system, we examined the role of eIF5A and its hypusine modification in translating polyproline sequence within long open reading frames. We found that polyproline motif inser...

journal_title：Journal of biochemistry

authors： Abe T,Nagai R,Shimazaki S,Kondo S,Nishimura S,Sakaguchi Y,Suzuki T,Imataka H,Tomita K,Takeuchi-Tomita N

更新日期：2020-05-01 00:00:00

abstract：:Kinetic studies of the two beta-glucosidase isozymes, GB-1 and GB-2, which were from the culture filtrate of a phytopathogenic fungus Pyricularia oryzae, revaled that the latter isozyme was an allosteric protein with two substrate binding sites. The homotropic effects of o- and m-nitrophenyl-beta-D-glucosides on GB-2 ...

journal_title：Journal of biochemistry

authors： Hirayama T,Nagayama H,Matsuda K

更新日期：1980-04-01 00:00:00

abstract：:The molecular masses of two of the four DNA polymerase alpha-primase complex subunit peptides from various mammalian cells have been compared through the use of specific monoclonal antibodies. One monoclonal antibody (E4) binds to 77-kDa peptide from HeLa cells and cognate peptides from other mammalian cells (monkey, ...

journal_title：Journal of biochemistry

authors： Kozu T,Nikaido K,Yagura T

更新日期：1990-04-01 00:00:00

abstract：:The complete peptide map of purified recombinant human interleukin 5 (rhIL-5) was determined to verify its primary structure, glycosylation sites, and disulfide bonding structure. Each peptide fragment generated by Achromobacter protease I (API) digestion was purified and characterized by amino acid analysis and amino...

journal_title：Journal of biochemistry

authors： Minamitake Y,Kodama S,Katayama T,Adachi H,Tanaka S,Tsujimoto M

更新日期：1990-02-01 00:00:00

abstract：:Homogeneous indanol dehydrogenase from monkey liver catalyzed the reversible conversion of 3 alpha- or 20 alpha-hydroxy groups of several bile acids and 5 beta-pregnanes to the corresponding 3- or 20-ketosteroids. The kcat values for the steroids determined at pH 7.4 were low, but the kcat/Km values for the 3-ketoster...

journal_title：Journal of biochemistry

authors： Hara A,Nakagawa M,Taniguchi H,Sawada H

更新日期：1989-11-01 00:00:00

abstract：:Tyr225 in the active site of Escherichia coli aspartate aminotransferase (AspAT) was replaced by phenylalanine or arginine by site-directed mutagenesis. X-ray crystallographic analysis of Y225F AspAT showed that the benzene ring of Phe225 was situated at the same position as the phenol ring of Tyr225 in wild-type AspA...

journal_title：Journal of biochemistry

authors： Inoue K,Kuramitsu S,Okamoto A,Hirotsu K,Higuchi T,Morino Y,Kagamiyama H

更新日期：1991-04-01 00:00:00

abstract：:Yeast mitochondrial ATP synthase has three regulatory proteins, ATPase inhibitor, 9K protein, and 15K protein. The 9K protein binds directly to purified F1-ATPase, as does the ATPase inhibitor, but the 15K protein does not [Hashimoto, T. et al. (1987) J. Biochem. 102, 685-692]. In the present study, we found that 15K ...

journal_title：Journal of biochemistry

authors： Hashimoto T,Yoshida Y,Tagawa K

更新日期：1990-07-01 00:00:00

abstract：:The dephosphorylation of phospho-amino acids with alkaline phosphatase (AlPase) from calf intestine or Escherichia coli and the phosphorylation of bovine serum albumin (BSA) with epidermal growth factor (EGF) receptor kinase from human A431 epidermoid carcinoma cells were investigated by 31P NMR spectroscopy. The init...

journal_title：Journal of biochemistry

authors： Takahashi K,Shimidzu M,Shindo H,Kawamoto T,Nishi M,Matsumoto U,Taniguchi S

更新日期：1987-05-01 00:00:00

abstract：:1. The addition of palmitic acid or myristic acid to a medium containing delipidated fetal bovine serum resulted in severe inhibition of V79-R cell growth. The degree of inhibition by palmitic acid was concentration dependent. Simultaneous addition of oleic acid protected the cells from the inhibition by palmitic acid...

journal_title：Journal of biochemistry

authors： Urade R,Kito M

更新日期：1982-05-01 00:00:00

abstract：:Ca2+-binding of S-100 protein was studied using a Ca2+ electrode at pH 6.80. In the presence of 0.1 M KCl and 10 mM MgCl2 (ionic strength 0.13), Ca2+-binding to S-100 protein occurred in three steps with positive cooperativity. The numbers of bound Ca2+ ions in the three steps were 2, 2, and 4. The Ca2+-binding consta...

journal_title：Journal of biochemistry

authors： Matsuda S

更新日期：1988-12-01 00:00:00

abstract：:Glutamine synthetase from a Gram-positive acid-fast bacterium, Mycobacterium smegmatis, was purified to homogeneity from cells grown with glycerol-bouillon medium. Electron micrographs of the enzyme revealed a dodecameric arrangement of its subunits in two superimposed hexagonal rings, similar to the structure of glut...

journal_title：Journal of biochemistry

authors： Kimura K,Yagi K,Matsuoka K

更新日期：1984-06-01 00:00:00

abstract：:Human HsRad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination. We have characterized the self-assembly of HsRad51 by measuring the fluorescence resonance energy transfer from the fluorescein-labeled protein to the rhodamine-labeled protein. Self-as...

journal_title：Journal of biochemistry

authors： Yoshioka K,Yumoto-Yoshioka Y,Fleury F,Takahashi M

更新日期：2003-05-01 00:00:00

abstract：:Studies with crude preparations obtained from a cysteine auxotroph of Saccharomyces cerevisiae showed that O-acetylserine sulfhydrylase could be separated from O-acetylhomoserine sulfhydrylase by chromatography on a DEAE-cellulose column and centrifugation in a sucrose density gradient. On the basis of sedimentation d...

journal_title：Journal of biochemistry

authors： Yamagata S

更新日期：1980-11-01 00:00:00

abstract：:Large amounts of extra proteins, X (in the envelope fraction) and X' (in the cytoplasmic fraction) were detected by SDS-polyacrylamide gel electrophoresis when DNA of Escherichia coli was damaged. These two proteins had the same apparent molecular weight (appproximately 40,000) and were produced under identical condit...

journal_title：Journal of biochemistry

authors： Yoda K,Nagai K,Tamura G

更新日期：1977-05-01 00:00:00